Rw3G027770

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000908
Physical Location & Seq
Reverse (-)
40080729 .. 40090082
9354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027770.1

Sequence Viewer

Length: 1248 bp
ATGCTGCCTTCCCTCTTCTCTCCCATTGAAACAAATTGGTTTGCACTTAACGACACCTCTCATACGACGCTATACTGTTTTCCGTGGTGTGAATCATTTCTCATTATTGAGACGACGGTTGTAAAATTTTCGTCTTCTAATATGAATTCAACCAACGGGGCAAAGCTGAAAAACGAAAAGAAAGGTAGAGGCTTTTCTCAAGTGGATACGAGGATTCCATTCTCACTAGTTCCACTTTACTTTTCCCCATTATTGTTCTTTAGGCCATGGAGAACGACGCGGTGGATCTGTTGGGTCGGGAGAGAAACTGGTCGGGTGGGATATGGGGTGCGGGAGGAGTGCGGAGGAGAGGGAGGCGGTGGAGTTGTTTCCACTGCAAATAACGGTGGGTTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTACGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACAGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCAGATGCGCCACCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCCCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATGCCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTGCGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCATGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

415

Amino Acids

46.27

Weight (kDa)

6.1

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 121 - 246 1.2e-32 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 260 - 359 6e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AccI GTMKAC 1 cut(s) 1140
AccII CGCG 1 cut(s) 280
AciI CCGC 5 cut(s) 280, 331, 342, 357, 862
AclWI GGATC 4 cut(s) 293, 438, 761, 983
AcsI RAATTY 5 cut(s) 125, 145, 725, 933, 1050
AfaI GTAC 3 cut(s) 573, 789, 1239
AfiI CCNNNNNNNGG 1 cut(s) 1165
AflIII ACRYGT 1 cut(s) 790
AgsI TTSAA 8 cut(s) 29, 150, 686, 744, 868, 898, 904, 1195
AhlI ACTAGT 1 cut(s) 226
AjnI CCWGG 2 cut(s) 779, 826
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 4 cut(s) 166, 457, 667, 733
AluI AGCT 4 cut(s) 166, 457, 667, 733
Alw26I GTCTC 3 cut(s) 104, 817, 1002
AlwI GGATC 4 cut(s) 293, 438, 761, 983
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 4 cut(s) 263, 596, 815, 953
ApeKI GCWGC 3 cut(s) 4, 664, 1061
ApoI RAATTY 5 cut(s) 125, 145, 725, 933, 1050
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 631
AsuHPI GGTGA 2 cut(s) 482, 521
AvaI CYCGRG 1 cut(s) 643
AxyI CCTNAGG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 676, 1048
BccI CCATC 4 cut(s) 440, 467, 686, 1193
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 2 cut(s) 781, 828
BciVI GTATCC 2 cut(s) 199, 511
BcoDI GTCTC 3 cut(s) 104, 817, 1002
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 2 cut(s) 662, 801
BfuI GTATCC 2 cut(s) 199, 511
BisI GCNGC 3 cut(s) 5, 665, 1062
BlsI GCNGC 3 cut(s) 6, 666, 1063
Bme1390I CCNGG 2 cut(s) 781, 828
BmeT110I CYCGRG 1 cut(s) 643
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 2 cut(s) 781, 828
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 2 cut(s) 408, 616
BmuI ACTGGG 1 cut(s) 731
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpmI CTGGAG 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 793
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 4 cut(s) 83, 266, 779, 1003
Bsc4I CCNNNNNNNGG 1 cut(s) 1165
Bse1I ACTGG 5 cut(s) 313, 559, 737, 1090, 1241
Bse21I CCTNAGG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 781, 828
BseDI CCNNGG 4 cut(s) 83, 266, 779, 1003
BseGI GGATG 1 cut(s) 1204
BseLI CCNNNNNNNGG 1 cut(s) 1165
BseMII CTCAG 4 cut(s) 417, 810, 960, 1152
BseNI ACTGG 5 cut(s) 313, 559, 737, 1090, 1241
BseRI GAGGAG 3 cut(s) 350, 360, 986
BseXI GCAGC 2 cut(s) 676, 1048
Bsh1236I CGCG 1 cut(s) 280
BshFI GGCC 4 cut(s) 265, 598, 817, 955
BsiHKCI CYCGRG 1 cut(s) 643
BslI CCNNNNNNNGG 1 cut(s) 1165
BsmAI GTCTC 3 cut(s) 104, 817, 1002
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 4 cut(s) 265, 598, 817, 955
Bso31I GGTCTC 1 cut(s) 817
BsoBI CYCGRG 1 cut(s) 643
Bsp1407I TGTACA 1 cut(s) 787
Bsp143I GATC 5 cut(s) 285, 430, 766, 835, 988
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 5 cut(s) 280, 331, 342, 357, 862
BspANI GGCC 4 cut(s) 265, 598, 817, 955
BspCNI CTCAG 4 cut(s) 418, 811, 961, 1153
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 975
BspMAI CTGCAG 2 cut(s) 666, 805
BspPI GGATC 4 cut(s) 293, 438, 761, 983
BspTNI GGTCTC 1 cut(s) 817
BsrGI TGTACA 1 cut(s) 787
BsrI ACTGG 5 cut(s) 313, 559, 737, 1090, 1241
BssECI CCNNGG 4 cut(s) 83, 266, 779, 1003
BssMI GATC 5 cut(s) 285, 430, 766, 835, 988
BssT1I CCWWGG 2 cut(s) 266, 1003
Bst2UI CCWGG 2 cut(s) 781, 828
Bst4CI ACNGT 8 cut(s) 77, 118, 386, 523, 550, 571, 619, 1138
Bst6I CTCTTC 2 cut(s) 20, 1032
BstAUI TGTACA 1 cut(s) 787
BstBAI YACGTR 1 cut(s) 793
BstC8I GCNNGC 3 cut(s) 538, 978, 1127
BstDEI CTNAG 6 cut(s) 426, 819, 839, 969, 1161, 1222
BstDSI CCRYGG 2 cut(s) 83, 266
BstF5I GGATG 1 cut(s) 1204
BstFNI CGCG 1 cut(s) 280
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 5 cut(s) 288, 433, 769, 838, 991
BstMAI GTCTC 3 cut(s) 104, 817, 1002
BstMBI GATC 5 cut(s) 285, 430, 766, 835, 988
BstNI CCWGG 2 cut(s) 781, 828
BstSCI CCNGG 2 cut(s) 779, 826
BstSFI CTRYAG 2 cut(s) 662, 801
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 2 cut(s) 676, 1048
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 4 cut(s) 285, 430, 766, 988
BstYI RGATCY 4 cut(s) 285, 430, 766, 988
Bsu36I CCTNAGG 1 cut(s) 426
BsuI GTATCC 2 cut(s) 199, 511
BsuRI GGCC 4 cut(s) 265, 598, 817, 955
BtgI CCRYGG 2 cut(s) 83, 266
BtgZI GCGATG 1 cut(s) 1188
BtsCI GGATG 1 cut(s) 1204
BtsI GCAGTG 2 cut(s) 372, 659
BtsIMutI CAGTG 4 cut(s) 372, 546, 566, 659
Cac8I GCNNGC 3 cut(s) 538, 978, 1127
CfoI GCGC 1 cut(s) 631
CseI GACGC 2 cut(s) 76, 286
Csp6I GTAC 3 cut(s) 572, 788, 1238
CviAII CATG 2 cut(s) 267, 1235
CviQI GTAC 3 cut(s) 572, 788, 1238
DdeI CTNAG 6 cut(s) 426, 819, 839, 969, 1161, 1222
DpnI GATC 5 cut(s) 287, 432, 768, 837, 990
DpnII GATC 5 cut(s) 285, 430, 766, 835, 988
DraI TTTAAA 1 cut(s) 1186
Eam1104I CTCTTC 2 cut(s) 20, 1032
EarI CTCTTC 2 cut(s) 20, 1032
Eco130I CCWWGG 2 cut(s) 266, 1003
Eco147I AGGCCT 2 cut(s) 598, 955
Eco31I GGTCTC 1 cut(s) 817
Eco32I GATATC 1 cut(s) 1029
Eco81I CCTNAGG 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 643
EcoRI GAATTC 2 cut(s) 145, 933
EcoRII CCWGG 2 cut(s) 779, 826
EcoRV GATATC 1 cut(s) 1029
EcoT14I CCWWGG 2 cut(s) 266, 1003
ErhI CCWWGG 2 cut(s) 266, 1003
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 2 cut(s) 270, 1238
FatI CATG 2 cut(s) 266, 1234
FauI CCCGC 1 cut(s) 324
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 1 cut(s) 1140
Fnu4HI GCNGC 3 cut(s) 5, 665, 1062
FokI GGATG 1 cut(s) 1211
Fsp4HI GCNGC 3 cut(s) 5, 665, 1062
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 630
GluI GCNGC 3 cut(s) 5, 665, 1062
GsuI CTGGAG 1 cut(s) 1002
HaeIII GGCC 4 cut(s) 265, 598, 817, 955
HgaI GACGC 2 cut(s) 76, 286
HhaI GCGC 1 cut(s) 631
Hin1II CATG 2 cut(s) 270, 1238
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HinfI GANTC 3 cut(s) 92, 214, 412
HphI GGTGA 2 cut(s) 482, 521
Hpy166II GTNNAC 5 cut(s) 393, 790, 845, 1141, 1180
Hpy188I TCNGA 4 cut(s) 404, 501, 625, 762
Hpy188III TCNNGA 4 cut(s) 298, 889, 923, 1032
Hpy8I GTNNAC 5 cut(s) 393, 790, 845, 1141, 1180
Hpy99I CGWCG 4 cut(s) 70, 118, 280, 483
HpyAV CCTTC 5 cut(s) 18, 713, 898, 1159, 1189
HpyCH4III ACNGT 8 cut(s) 77, 118, 386, 523, 550, 571, 619, 1138
HpyCH4IV ACGT 1 cut(s) 792
HpyCH4V TGCA 8 cut(s) 44, 377, 440, 611, 664, 803, 962, 1064
HpyF3I CTNAG 6 cut(s) 426, 819, 839, 969, 1161, 1222
HpySE526I ACGT 1 cut(s) 792
Hsp92II CATG 2 cut(s) 270, 1238
HspAI GCGC 1 cut(s) 629
Kzo9I GATC 5 cut(s) 285, 430, 766, 835, 988
LmnI GCTCC 3 cut(s) 738, 973, 983
Lsp1109I GCAGC 2 cut(s) 676, 1048
LweI GCATC 2 cut(s) 408, 616
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 792
MaeIII GTNAC 3 cut(s) 527, 657, 808
MalI GATC 5 cut(s) 287, 432, 768, 837, 990
MboI GATC 5 cut(s) 285, 430, 766, 835, 988
MboII GAAGA 4 cut(s) 7, 126, 579, 1049
MfeI CAATTG 1 cut(s) 945
MflI RGATCY 4 cut(s) 285, 430, 766, 988
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 7 cut(s) 48, 465, 729, 917, 965, 1104, 1185
MspR9I CCNGG 2 cut(s) 781, 828
MunI CAATTG 1 cut(s) 945
MvaI CCWGG 2 cut(s) 781, 828
MvnI CGCG 1 cut(s) 280
NcoI CCATGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 442
NdeII GATC 5 cut(s) 285, 430, 766, 835, 988
NlaIII CATG 2 cut(s) 270, 1238
NlaIV GGNNCC 1 cut(s) 975
NmeAIII GCCGAG 1 cut(s) 793
NmuCI GTSAC 1 cut(s) 657
PceI AGGCCT 2 cut(s) 598, 955
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 3 cut(s) 92, 214, 412
PkrI GCNGC 3 cut(s) 6, 666, 1063
Ppu21I YACGTR 1 cut(s) 793
PsiI TTATAA 1 cut(s) 486
Psp6I CCWGG 2 cut(s) 779, 826
PspGI CCWGG 2 cut(s) 779, 826
PspN4I GGNNCC 1 cut(s) 975
PstI CTGCAG 2 cut(s) 666, 805
PsuI RGATCY 4 cut(s) 285, 430, 766, 988
RsaI GTAC 3 cut(s) 573, 789, 1239
RsaNI GTAC 3 cut(s) 572, 788, 1238
SaqAI TTAA 7 cut(s) 48, 465, 729, 917, 965, 1104, 1185
SatI GCNGC 3 cut(s) 5, 665, 1062
Sau3AI GATC 5 cut(s) 285, 430, 766, 835, 988
ScrFI CCNGG 2 cut(s) 781, 828
SfaNI GCATC 2 cut(s) 408, 616
SfcI CTRYAG 2 cut(s) 662, 801
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SpeI ACTAGT 1 cut(s) 226
SseBI AGGCCT 2 cut(s) 598, 955
SsiI CCGC 5 cut(s) 280, 331, 342, 357, 862
SspI AATATT 1 cut(s) 652
SspMI CTAG 1 cut(s) 227
StuI AGGCCT 2 cut(s) 598, 955
StyD4I CCNGG 2 cut(s) 779, 826
StyI CCWWGG 2 cut(s) 266, 1003
TaaI ACNGT 8 cut(s) 77, 118, 386, 523, 550, 571, 619, 1138
TaiI ACGT 1 cut(s) 795
TatI WGTACW 1 cut(s) 787
TfiI GAWTC 3 cut(s) 92, 214, 412
Tru1I TTAA 7 cut(s) 48, 465, 729, 917, 965, 1104, 1185
Tru9I TTAA 7 cut(s) 48, 465, 729, 917, 965, 1104, 1185
TscAI CASTG 4 cut(s) 379, 553, 566, 666
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 3 cut(s) 4, 664, 1061
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 1 cut(s) 158
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 4 cut(s) 379, 553, 566, 666
XapI RAATTY 5 cut(s) 125, 145, 725, 933, 1050
XmiI GTMKAC 1 cut(s) 1140
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.