Rw5G021990

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000908
Physical Location & Seq
Forward (+)
30018965 .. 30030347
11383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021990.1

Sequence Viewer

Length: 1221 bp
ATGACCTGGCATTTTAATTCCATCATTTTTCTCATAGCCAGAAGTGAACTCTTTGCTAATTGGTGCATGGTAGAATACATATTTTTCGTAGAGACTCCTGTTATTATCTCGGGATATGATCTTTATGCTTATTATAATTTGGGATTCAGGTATCATGTCCCCCCCCCCCCATTGTATTCTACAGTTTCCCACACTCCCACAAAGACACCACGACACAGAGACACCCCGACACCCCGACACCTCCCCTCGCCGTCTCTCCTTTCCATGGGCCTCCCCTTCTCAGCCCCTCTCTTCGACCGAGTTGTTTCCACTGCAAATAACGGTGGGTTTACAAGCATCAAAACAAAGAATCTATCAACACCTAAGGATCTCTCTGCATATGATGGTTTAGAGCTGCGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACAGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCAGATGCGCCACCATTTGACCCAAGCAATATGTGTCACTGCAGGCAATTTTCTACTGCCACTTTTTCTCACTCATTCTTTCACTTTAAGATTTTATTCCATCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGAGAAGAGGTTGCTCAAATTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATACCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTACGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCCTGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

406

Amino Acids

45.87

Weight (kDa)

8.38

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 98 - 198 7.2e-28 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 135, 423
AccI GTMKAC 1 cut(s) 1113
AciI CCGC 1 cut(s) 856
AclWI GGATC 2 cut(s) 375, 755
AcsI RAATTY 2 cut(s) 719, 921
AfaI GTAC 3 cut(s) 510, 783, 1212
AfiI CCNNNNNNNGG 2 cut(s) 265, 1138
AflIII ACRYGT 1 cut(s) 784
AgsI TTSAA 6 cut(s) 680, 738, 862, 892, 898, 1168
AjnI CCWGG 3 cut(s) 5, 773, 820
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AluBI AGCT 2 cut(s) 394, 727
AluI AGCT 2 cut(s) 394, 727
Alw26I GTCTC 5 cut(s) 86, 213, 258, 811, 990
AlwI GGATC 2 cut(s) 375, 755
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 4 cut(s) 268, 533, 809, 941
ApeKI GCWGC 2 cut(s) 394, 1034
ApoI RAATTY 2 cut(s) 719, 921
AspLEI GCGC 2 cut(s) 399, 568
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 419, 458
AvaI CYCGRG 1 cut(s) 109
AxyI CCTNAGG 1 cut(s) 363
BbvI GCAGC 2 cut(s) 381, 1021
BccI CCATC 6 cut(s) 29, 377, 404, 666, 680, 1166
BceAI ACGGC 2 cut(s) 235, 527
BciT130I CCWGG 3 cut(s) 7, 775, 822
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 5 cut(s) 86, 213, 258, 811, 990
BfmI CTRYAG 3 cut(s) 180, 598, 795
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 976
BisI GCNGC 2 cut(s) 395, 1035
BlsI GCNGC 2 cut(s) 396, 1036
Bme1390I CCNGG 3 cut(s) 7, 775, 822
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 963
BmrFI CCNGG 3 cut(s) 7, 775, 822
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 2 cut(s) 345, 553
BmuI ACTGGG 1 cut(s) 725
BplI GAGNNNNNCTC 2 cut(s) 963, 995
BpmI CTGGAG 1 cut(s) 990
BsaAI YACGTR 1 cut(s) 787
BsaI GGTCTC 1 cut(s) 811
BsaJI CCNNGG 3 cut(s) 264, 773, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 1138
Bse1I ACTGG 4 cut(s) 496, 731, 1063, 1214
Bse21I CCTNAGG 1 cut(s) 363
BseBI CCWGG 3 cut(s) 7, 775, 822
BseDI CCNNGG 3 cut(s) 264, 773, 991
BseGI GGATG 1 cut(s) 1177
BseLI CCNNNNNNNGG 2 cut(s) 265, 1138
BseMII CTCAG 4 cut(s) 294, 804, 948, 1125
BseNI ACTGG 4 cut(s) 496, 731, 1063, 1214
BseRI GAGGAG 1 cut(s) 974
BseXI GCAGC 2 cut(s) 381, 1021
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 4 cut(s) 270, 535, 811, 943
BsiEI CGRYCG 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 265, 1138
BsmAI GTCTC 5 cut(s) 86, 213, 258, 811, 990
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 4 cut(s) 270, 535, 811, 943
Bso31I GGTCTC 1 cut(s) 811
BsoBI CYCGRG 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 5 cut(s) 118, 367, 760, 829, 976
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 1 cut(s) 856
BspANI GGCC 4 cut(s) 270, 535, 811, 943
BspCNI CTCAG 4 cut(s) 293, 805, 949, 1126
BspLI GGNNCC 1 cut(s) 963
BspMAI CTGCAG 2 cut(s) 602, 799
BspPI GGATC 2 cut(s) 375, 755
BspTNI GGTCTC 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 4 cut(s) 496, 731, 1063, 1214
BssECI CCNNGG 3 cut(s) 264, 773, 991
BssMI GATC 5 cut(s) 118, 367, 760, 829, 976
BssT1I CCWWGG 2 cut(s) 264, 991
Bst2UI CCWGG 3 cut(s) 7, 775, 822
Bst4CI ACNGT 7 cut(s) 184, 323, 460, 487, 508, 556, 1111
Bst6I CTCTTC 2 cut(s) 296, 1006
BstAUI TGTACA 1 cut(s) 781
BstBAI YACGTR 1 cut(s) 787
BstC8I GCNNGC 4 cut(s) 475, 602, 966, 1100
BstDEI CTNAG 7 cut(s) 280, 363, 813, 833, 957, 1134, 1195
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 1177
BstHHI GCGC 2 cut(s) 399, 568
BstKTI GATC 5 cut(s) 121, 370, 763, 832, 979
BstMAI GTCTC 5 cut(s) 86, 213, 258, 811, 990
BstMBI GATC 5 cut(s) 118, 367, 760, 829, 976
BstMCI CGRYCG 1 cut(s) 298
BstNI CCWGG 3 cut(s) 7, 775, 822
BstSCI CCNGG 3 cut(s) 5, 773, 820
BstSFI CTRYAG 3 cut(s) 180, 598, 795
BstV1I GCAGC 2 cut(s) 381, 1021
BstX2I RGATCY 3 cut(s) 367, 760, 976
BstYI RGATCY 3 cut(s) 367, 760, 976
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 270, 535, 811, 943
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 1 cut(s) 1177
BtsI GCAGTG 2 cut(s) 309, 595
BtsIMutI CAGTG 4 cut(s) 309, 483, 503, 595
Cac8I GCNNGC 4 cut(s) 475, 602, 966, 1100
CfoI GCGC 2 cut(s) 399, 568
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 509, 782, 1211
CviAII CATG 3 cut(s) 67, 155, 265
CviQI GTAC 3 cut(s) 509, 782, 1211
DdeI CTNAG 7 cut(s) 280, 363, 813, 833, 957, 1134, 1195
DpnI GATC 5 cut(s) 120, 369, 762, 831, 978
DpnII GATC 5 cut(s) 118, 367, 760, 829, 976
DraI TTTAAA 1 cut(s) 1159
Eam1104I CTCTTC 2 cut(s) 296, 1006
EarI CTCTTC 2 cut(s) 296, 1006
Eco130I CCWWGG 2 cut(s) 264, 991
Eco147I AGGCCT 2 cut(s) 535, 943
Eco31I GGTCTC 1 cut(s) 811
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 921
EcoRII CCWGG 3 cut(s) 5, 773, 820
EcoT14I CCWWGG 2 cut(s) 264, 991
ErhI CCWWGG 2 cut(s) 264, 991
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 3 cut(s) 70, 158, 268
FaqI GGGAC 1 cut(s) 143
FatI CATG 3 cut(s) 66, 154, 264
FauNDI CATATG 1 cut(s) 379
FblI GTMKAC 1 cut(s) 1113
Fnu4HI GCNGC 2 cut(s) 395, 1035
FokI GGATG 1 cut(s) 1184
Fsp4HI GCNGC 2 cut(s) 395, 1035
GlaI GCGC 2 cut(s) 398, 567
GluI GCNGC 2 cut(s) 395, 1035
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 4 cut(s) 270, 535, 811, 943
HhaI GCGC 2 cut(s) 399, 568
Hin1II CATG 3 cut(s) 70, 158, 268
Hin6I GCGC 2 cut(s) 397, 566
HinP1I GCGC 2 cut(s) 397, 566
HinfI GANTC 3 cut(s) 94, 144, 349
HphI GGTGA 2 cut(s) 419, 458
Hpy166II GTNNAC 6 cut(s) 47, 330, 784, 839, 1114, 1153
Hpy188I TCNGA 3 cut(s) 438, 562, 756
Hpy188III TCNNGA 3 cut(s) 111, 883, 911
Hpy8I GTNNAC 6 cut(s) 47, 330, 784, 839, 1114, 1153
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 5 cut(s) 286, 707, 892, 1132, 1162
HpyCH4III ACNGT 7 cut(s) 184, 323, 460, 487, 508, 556, 1111
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 8 cut(s) 66, 314, 377, 548, 600, 797, 950, 1037
HpyF3I CTNAG 7 cut(s) 280, 363, 813, 833, 957, 1134, 1195
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 3 cut(s) 70, 158, 268
HspAI GCGC 2 cut(s) 397, 566
Kzo9I GATC 5 cut(s) 118, 367, 760, 829, 976
LmnI GCTCC 3 cut(s) 732, 961, 971
Lsp1109I GCAGC 2 cut(s) 381, 1021
LweI GCATC 2 cut(s) 345, 553
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 3 cut(s) 464, 593, 802
MalI GATC 5 cut(s) 120, 369, 762, 831, 978
MboI GATC 5 cut(s) 118, 367, 760, 829, 976
MboII GAAGA 3 cut(s) 283, 516, 1023
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 3 cut(s) 367, 760, 976
MlyI GAGTC 1 cut(s) 88
MseI TTAA 8 cut(s) 15, 402, 645, 723, 905, 953, 1077, 1158
MspR9I CCNGG 3 cut(s) 7, 775, 822
MunI CAATTG 1 cut(s) 933
MvaI CCWGG 3 cut(s) 7, 775, 822
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 379
NdeII GATC 5 cut(s) 118, 367, 760, 829, 976
NlaIII CATG 3 cut(s) 70, 158, 268
NlaIV GGNNCC 1 cut(s) 963
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 1 cut(s) 593
PceI AGGCCT 2 cut(s) 535, 943
PfeI GAWTC 2 cut(s) 144, 349
PkrI GCNGC 2 cut(s) 396, 1036
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 787
PsiI TTATAA 2 cut(s) 135, 423
Psp6I CCWGG 3 cut(s) 5, 773, 820
PspGI CCWGG 3 cut(s) 5, 773, 820
PspN4I GGNNCC 1 cut(s) 963
PspPI GGNCC 1 cut(s) 268
PstI CTGCAG 2 cut(s) 602, 799
PsuI RGATCY 3 cut(s) 367, 760, 976
RsaI GTAC 3 cut(s) 510, 783, 1212
RsaNI GTAC 3 cut(s) 509, 782, 1211
SaqAI TTAA 8 cut(s) 15, 402, 645, 723, 905, 953, 1077, 1158
SatI GCNGC 2 cut(s) 395, 1035
Sau3AI GATC 5 cut(s) 118, 367, 760, 829, 976
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 3 cut(s) 7, 775, 822
SfaNI GCATC 2 cut(s) 345, 553
SfcI CTRYAG 3 cut(s) 180, 598, 795
SseBI AGGCCT 2 cut(s) 535, 943
SsiI CCGC 1 cut(s) 856
StuI AGGCCT 2 cut(s) 535, 943
StyD4I CCNGG 3 cut(s) 5, 773, 820
StyI CCWWGG 2 cut(s) 264, 991
TaaI ACNGT 7 cut(s) 184, 323, 460, 487, 508, 556, 1111
TaiI ACGT 1 cut(s) 789
TaqI TCGA 1 cut(s) 294
TaqII GACCGA 1 cut(s) 312
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 2 cut(s) 144, 349
Tru1I TTAA 8 cut(s) 15, 402, 645, 723, 905, 953, 1077, 1158
Tru9I TTAA 8 cut(s) 15, 402, 645, 723, 905, 953, 1077, 1158
TscAI CASTG 4 cut(s) 316, 490, 503, 602
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 2 cut(s) 394, 1034
Tsp45I GTSAC 1 cut(s) 593
TspRI CASTG 4 cut(s) 316, 490, 503, 602
XapI RAATTY 2 cut(s) 719, 921
XmiI GTMKAC 1 cut(s) 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.