MD05G1302400.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006679
Physical Location & Seq
Reverse (-)
43483961 .. 43487790
3830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1302400.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGACTTTTACGGTTTTAACTCCAAATCTTCATTTAATCCGAGCTTATTCGACCCTTTTAAGCATGGCGCAAGTGGGTTCGGCGGGGCTTTGCAGGGCGGTGCTGCGGCGGCTCTGCCTCACTCACTGGTTTTGGACAGTGAGAAAGGCGAGCTCGTTAAGGCTCCTGCGAGAGTGGGGAAAAAAGGGGTGTCTGAGGCTAAGGCTTTGGCGGCTTTGAAGAATCACAGTGAGGCCGAAAGGAGGAGGAGAGAGAGAATCAATGCACATCTGTCTACGCTTCGCGGTCTCGTGCCCTGCACTGAAAAGATGGACAAAGCCGCGTTACTTGCTGCAGTTATCAGCCAAGTGAAGGAACTGAAGAAGGATGCTCTAGAATCCAGTAAGGGGTTTCTCATCCCAGTTGATGACGATGAAGTGAAAGTTGAACCCTATGTCATTGGGGCAGGGGATGGAACCATATCTGTGAAGGCATCCGTCTGCTGTGAATACCGGTCTGAGCATCTCTCTGACCTAAGAGAAGCCCTCGACTCCCTCCACTTAAAAATGGTGAAGGCGGAAATAGCGACTTTGGGAAACCGAGTGAAAAATGTATTCGTTCTCACCAGCTTCAAAAAGGACAGCAACGATGCCAATACTGAAGCATACGAACTTCTTGCAAGTTCGGTTCACCAGGTCCTGAGTTCCGTCCTCGATAAGGCTTCGCCCTCACCAGAATACTCCCCGAGAACAACGCTGCCGAGCAAAAGGCAAAGGCTCTCTTACTTCGATAAAATCGATACCTCGAGCTCATCCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

267

Amino Acids

28.73

Weight (kDa)

8.61

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 74 - 120 2.7e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 275
AccII CGCG 2 cut(s) 285, 323
AciI CCGC 8 cut(s) 84, 99, 107, 110, 212, 285, 321, 557
AcuI CTGAAG 2 cut(s) 380, 660
AfiI CCNNNNNNNGG 4 cut(s) 243, 352, 387, 697
AgeI ACCGGT 1 cut(s) 492
AgsI TTSAA 3 cut(s) 220, 428, 613
AjnI CCWGG 1 cut(s) 672
AluBI AGCT 4 cut(s) 45, 154, 609, 789
AluI AGCT 4 cut(s) 45, 154, 609, 789
Alw21I GWGCWC 2 cut(s) 156, 791
Alw26I GTCTC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 679
Ama87I CYCGRG 2 cut(s) 724, 784
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 104, 332, 736
AsiGI ACCGGT 1 cut(s) 492
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 4 cut(s) 562, 595, 662, 702
AvaI CYCGRG 2 cut(s) 724, 784
AvaII GGWCC 1 cut(s) 676
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 156, 791
BauI CACGAG 2 cut(s) 290, 796
Bbv12I GWGCWC 2 cut(s) 156, 791
BbvI GCAGC 3 cut(s) 91, 319, 723
BccI CCATC 2 cut(s) 304, 446
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 1 cut(s) 674
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 7 cut(s) 105, 108, 111, 213, 321, 333, 737
BlsI GCNGC 7 cut(s) 106, 109, 112, 214, 322, 334, 738
Bme1390I CCNGG 1 cut(s) 674
Bme18I GGWCC 1 cut(s) 676
BmeT110I CYCGRG 2 cut(s) 724, 784
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 2 cut(s) 165, 457
BmrFI CCNGG 1 cut(s) 674
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 4 cut(s) 358, 482, 511, 619
BmuI ACTGGG 1 cut(s) 395
BplI GAGNNNNNCTC 4 cut(s) 491, 523, 510, 542
Bpu10I CCTNAGC 1 cut(s) 201
Bsa29I ATCGAT 1 cut(s) 777
BsaI GGTCTC 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 492
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 352, 387, 697
Bse118I RCCGGY 1 cut(s) 492
Bse1I ACTGG 3 cut(s) 132, 381, 401
BseBI CCWGG 1 cut(s) 674
BseCI ATCGAT 1 cut(s) 777
BseGI GGATG 5 cut(s) 373, 396, 457, 473, 791
BseLI CCNNNNNNNGG 4 cut(s) 243, 352, 387, 697
BseMII CTCAG 3 cut(s) 186, 489, 671
BseNI ACTGG 3 cut(s) 132, 381, 401
BseRI GAGGAG 2 cut(s) 259, 262
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 3 cut(s) 91, 319, 723
BsgI GTGCAG 1 cut(s) 283
Bsh1236I CGCG 2 cut(s) 285, 323
BshFI GGCC 1 cut(s) 236
BshTI ACCGGT 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 2 cut(s) 156, 791
BsiHKCI CYCGRG 2 cut(s) 724, 784
BsiSI CCGG 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 243, 352, 387, 697
BsmAI GTCTC 1 cut(s) 293
BsnI GGCC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 2 cut(s) 724, 784
Bsp1286I GDGCHC 3 cut(s) 156, 297, 791
BspACI CCGC 8 cut(s) 84, 99, 107, 110, 212, 285, 321, 557
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 3 cut(s) 187, 490, 672
BspDI ATCGAT 1 cut(s) 777
BspFNI CGCG 2 cut(s) 285, 323
BspLI GGNNCC 2 cut(s) 165, 457
BspMAI CTGCAG 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 492
BsrI ACTGG 3 cut(s) 132, 381, 401
BssAI RCCGGY 1 cut(s) 492
BssSI CACGAG 2 cut(s) 290, 796
Bst2BI CACGAG 2 cut(s) 290, 796
Bst2UI CCWGG 1 cut(s) 674
Bst4CI ACNGT 3 cut(s) 14, 140, 230
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 5 cut(s) 195, 201, 498, 515, 680
BstENI CCTNNNNNAGG 1 cut(s) 695
BstF5I GGATG 5 cut(s) 373, 396, 457, 473, 791
BstFNI CGCG 2 cut(s) 285, 323
BstHHI GCGC 1 cut(s) 71
BstMAI GTCTC 1 cut(s) 293
BstMWI GCNNNNNNNGC 4 cut(s) 110, 212, 329, 563
BstNI CCWGG 1 cut(s) 674
BstSCI CCNGG 1 cut(s) 672
BstSFI CTRYAG 1 cut(s) 333
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 2 cut(s) 285, 323
BstV1I GCAGC 3 cut(s) 91, 319, 723
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 1 cut(s) 236
BsuTUI ATCGAT 1 cut(s) 777
BtsCI GGATG 5 cut(s) 373, 396, 457, 473, 791
BtsIMutI CAGTG 4 cut(s) 125, 145, 235, 300
Cac8I GCNNGC 1 cut(s) 152
CaiI CAGNNNCTG 1 cut(s) 679
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 676
ClaI ATCGAT 1 cut(s) 777
CsiI ACCWGGT 1 cut(s) 672
CspAI ACCGGT 1 cut(s) 492
CviAII CATG 1 cut(s) 65
DdeI CTNAG 5 cut(s) 195, 201, 498, 515, 680
EciI GGCGGA 1 cut(s) 572
Ecl136II GAGCTC 2 cut(s) 154, 789
Eco24I GRGCYC 2 cut(s) 156, 791
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 676
Eco53kI GAGCTC 2 cut(s) 154, 789
Eco57I CTGAAG 2 cut(s) 380, 660
Eco88I CYCGRG 2 cut(s) 724, 784
EcoICRI GAGCTC 2 cut(s) 154, 789
EcoNI CCTNNNNNAGG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 672
EcoT38I GRGCYC 2 cut(s) 156, 791
FaeI CATG 1 cut(s) 68
FaiI YATR 4 cut(s) 66, 435, 461, 646
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FatI CATG 1 cut(s) 64
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 7 cut(s) 105, 108, 111, 213, 321, 333, 737
FokI GGATG 5 cut(s) 380, 383, 460, 464, 778
FriOI GRGCYC 2 cut(s) 156, 791
Fsp4HI GCNGC 7 cut(s) 105, 108, 111, 213, 321, 333, 737
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 70
GluI GCNGC 7 cut(s) 105, 108, 111, 213, 321, 333, 737
HaeIII GGCC 1 cut(s) 236
HapII CCGG 1 cut(s) 493
HhaI GCGC 1 cut(s) 71
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HinfI GANTC 4 cut(s) 223, 258, 377, 530
HpaII CCGG 1 cut(s) 493
HphI GGTGA 4 cut(s) 562, 595, 662, 702
Hpy166II GTNNAC 2 cut(s) 276, 670
Hpy188I TCNGA 4 cut(s) 42, 196, 499, 511
Hpy188III TCNNGA 3 cut(s) 374, 679, 798
Hpy8I GTNNAC 2 cut(s) 276, 670
HpyAV CCTTC 4 cut(s) 346, 358, 463, 547
HpyCH4III ACNGT 3 cut(s) 14, 140, 230
HpyCH4V TGCA 5 cut(s) 94, 266, 300, 335, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 212, 329, 563
HpyF3I CTNAG 5 cut(s) 195, 201, 498, 515, 680
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 3 cut(s) 91, 319, 723
LweI GCATC 4 cut(s) 358, 482, 511, 619
MabI ACCWGGT 1 cut(s) 672
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 324
MboII GAAGA 3 cut(s) 21, 232, 373
MhlI GDGCHC 3 cut(s) 156, 297, 791
MlyI GAGTC 1 cut(s) 524
MseI TTAA 5 cut(s) 18, 36, 60, 159, 542
MslI CAYNNNNRTG 2 cut(s) 464, 796
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 674
MvaI CCWGG 1 cut(s) 674
MvnI CGCG 2 cut(s) 285, 323
MwoI GCNNNNNNNGC 4 cut(s) 110, 212, 329, 563
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 2 cut(s) 165, 457
NmeAIII GCCGAG 1 cut(s) 765
PaeR7I CTCGAG 1 cut(s) 784
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PfeI GAWTC 3 cut(s) 223, 258, 377
PinAI ACCGGT 1 cut(s) 492
PkrI GCNGC 7 cut(s) 106, 109, 112, 214, 322, 334, 738
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PpuMI RGGWCCY 1 cut(s) 676
Psp124BI GAGCTC 2 cut(s) 156, 791
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 1 cut(s) 672
PspGI CCWGG 1 cut(s) 672
PspN4I GGNNCC 2 cut(s) 165, 457
PspPI GGNCC 1 cut(s) 676
PspPPI RGGWCCY 1 cut(s) 676
PspXI VCTCGAGB 1 cut(s) 784
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 679
RseI CAYNNNNRTG 2 cut(s) 464, 796
SacI GAGCTC 2 cut(s) 156, 791
SaqAI TTAA 5 cut(s) 18, 36, 60, 159, 542
SatI GCNGC 7 cut(s) 105, 108, 111, 213, 321, 333, 737
Sau96I GGNCC 1 cut(s) 676
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 674
SduI GDGCHC 3 cut(s) 156, 297, 791
SetI ASST 7 cut(s) 47, 156, 516, 611, 678, 785, 791
SexAI ACCWGGT 1 cut(s) 672
SfaNI GCATC 4 cut(s) 358, 482, 511, 619
SfcI CTRYAG 1 cut(s) 333
Sfr274I CTCGAG 1 cut(s) 784
SinI GGWCC 1 cut(s) 676
SlaI CTCGAG 1 cut(s) 784
SmiMI CAYNNNNRTG 2 cut(s) 464, 796
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SsiI CCGC 8 cut(s) 84, 99, 107, 110, 212, 285, 321, 557
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 2 cut(s) 156, 791
StyD4I CCNGG 1 cut(s) 672
TaaI ACNGT 3 cut(s) 14, 140, 230
TaqI TCGA 6 cut(s) 51, 528, 693, 768, 777, 785
TauI GCSGC 4 cut(s) 110, 113, 215, 323
TfiI GAWTC 3 cut(s) 223, 258, 377
Tru1I TTAA 5 cut(s) 18, 36, 60, 159, 542
Tru9I TTAA 5 cut(s) 18, 36, 60, 159, 542
TscAI CASTG 4 cut(s) 132, 145, 235, 307
TseI GCWGC 3 cut(s) 104, 332, 736
TspDTI ATGAA 2 cut(s) 21, 429
TspGWI ACGGA 2 cut(s) 466, 676
TspRI CASTG 4 cut(s) 132, 145, 235, 307
VpaK11BI GGWCC 1 cut(s) 676
XagI CCTNNNNNAGG 1 cut(s) 695
XbaI TCTAGA 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 784
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.