MD10G1281100.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006679
Physical Location & Seq
Reverse (-)
37180790 .. 37184568
3779 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1281100.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGATTATTACAGTTTTAACTCAAAATCTTCATTTGGGTCGGATTTCTCGAGCTTGTTCGACCCTTTTAAGCATGACGTAAGTGGGTTCGGCGGGGCTTTGCGAGGCGGCGCTGCGGCGAAACTGCCTCACTCTCTTGTTTTGGACAGTGAGAAAGGCGAGCTTGTTAAGGCTCCGGCGAGAGTGGGGAAAAAAGAGCTGCCTGAGGCCAAGGCTTTGTTGGCTTTGAAGAATCACAGTGAGGCTGAAAGGAGGAGGAGAGAGAGAATCAATGCACATCTCTCTACACTTCGCGGTCTGGTGCCCTGCACTGAGAAGATGGACAAAGCCACATTACTTGCAGCAGTTATCAGCCAAGTGAAAGAACTGAAGAAGGATGCCCTAGAATCCAGTAAGGGGTTTCTCATCCCAGTTGATGCCGATGAAGTGAAAATCGAACCCTATGTTGCGGCAGGGGATGGAACCATATCTGTGAAGGCATCCGTCTGCTGTGAATACCGGTCTGAGCTTCTCTCTGACCTAAGAGAAGCCCTCGACTCCCTCCACTTAAAAATTGTGAAAGCAGAAATAGCGACCTTGGGAAACCGAGTGAAAAATGTATTCGTTCTCACCAGCTCCAAACCGGGAAGCAATGATTCTGATGCTGAAGCATACGAACTTCTTGCAAGTTCGGTTCACCAGGTCCTGAGTTCCATCCTCGATAAAGCATCGGCCTCACCAGAATACTCGCCAAGAACAACGCTGCCGAGCAAAAGGCGAAGGCTCTCTTACTTCGATAAAATCGATACCTCGAGCTCATCCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

269

Amino Acids

29.08

Weight (kDa)

8.6

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 77 - 123 2.2e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 301
AccII CGCG 1 cut(s) 294
AciI CCGC 5 cut(s) 93, 108, 116, 294, 449
AcuI CTGAAG 2 cut(s) 389, 666
AfiI CCNNNNNNNGG 1 cut(s) 396
AgeI ACCGGT 1 cut(s) 498
AgsI TTSAA 1 cut(s) 229
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 6 cut(s) 54, 163, 199, 508, 615, 795
AluI AGCT 6 cut(s) 54, 163, 199, 508, 615, 795
Alw21I GWGCWC 1 cut(s) 797
AlwNI CAGNNNCTG 1 cut(s) 685
Ama87I CYCGRG 2 cut(s) 49, 790
AoxI GGCC 2 cut(s) 207, 711
ApeKI GCWGC 4 cut(s) 113, 199, 341, 742
AsiGI ACCGGT 1 cut(s) 498
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 3 cut(s) 601, 668, 708
AvaI CYCGRG 2 cut(s) 49, 790
AvaII GGWCC 1 cut(s) 682
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 797
BauI CACGAG 1 cut(s) 802
Bbv12I GWGCWC 1 cut(s) 797
BbvI GCAGC 4 cut(s) 100, 186, 353, 729
BccI CCATC 3 cut(s) 313, 452, 701
BcgI CGANNNNNNTGC 2 cut(s) 644, 678
BciT130I CCWGG 1 cut(s) 680
BcnI CCSGG 1 cut(s) 624
BfaI CTAG 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 7 cut(s) 109, 114, 117, 200, 342, 450, 743
BlsI GCNGC 7 cut(s) 110, 115, 118, 201, 343, 451, 744
Bme1390I CCNGG 2 cut(s) 624, 680
Bme18I GGWCC 1 cut(s) 682
BmeT110I CYCGRG 2 cut(s) 49, 790
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 3 cut(s) 174, 303, 463
BmrFI CCNGG 2 cut(s) 624, 680
BmrI ACTGGG 1 cut(s) 404
BmsI GCATC 5 cut(s) 367, 406, 488, 631, 716
BmuI ACTGGG 1 cut(s) 404
BplI GAGNNNNNCTC 4 cut(s) 497, 529, 516, 548
BpuMI CCSGG 1 cut(s) 624
Bsa29I ATCGAT 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 210, 576
BsaWI WCCGGW 1 cut(s) 498
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 2 cut(s) 390, 410
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 210, 576
BseGI GGATG 6 cut(s) 382, 405, 463, 479, 693, 797
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 4 cut(s) 195, 303, 495, 677
BseNI ACTGG 2 cut(s) 390, 410
BseRI GAGGAG 2 cut(s) 268, 271
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 4 cut(s) 100, 186, 353, 729
BsgI GTGCAG 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 209, 713
BshNI GGYRCC 1 cut(s) 301
BshTI ACCGGT 1 cut(s) 498
BshVI ATCGAT 1 cut(s) 783
BsiHKAI GWGCWC 1 cut(s) 797
BsiHKCI CYCGRG 2 cut(s) 49, 790
BsiSI CCGG 3 cut(s) 176, 499, 623
BslI CCNNNNNNNGG 1 cut(s) 396
BsnI GGCC 2 cut(s) 209, 713
BsoBI CYCGRG 2 cut(s) 49, 790
Bsp1286I GDGCHC 2 cut(s) 306, 797
BspACI CCGC 5 cut(s) 93, 108, 116, 294, 449
BspANI GGCC 2 cut(s) 209, 713
BspCNI CTCAG 4 cut(s) 196, 304, 496, 678
BspDI ATCGAT 1 cut(s) 783
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 174, 303, 463
BspT107I GGYRCC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 2 cut(s) 390, 410
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 210, 576
BssSI CACGAG 1 cut(s) 802
BssT1I CCWWGG 2 cut(s) 210, 576
Bst2BI CACGAG 1 cut(s) 802
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 14, 149, 239
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 5 cut(s) 204, 312, 504, 521, 686
BstF5I GGATG 6 cut(s) 382, 405, 463, 479, 693, 797
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 221, 569
BstNI CCWGG 1 cut(s) 680
BstSCI CCNGG 2 cut(s) 622, 678
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 4 cut(s) 100, 186, 353, 729
Bsu15I ATCGAT 1 cut(s) 783
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 2 cut(s) 209, 713
BsuTUI ATCGAT 1 cut(s) 783
BtsCI GGATG 6 cut(s) 382, 405, 463, 479, 693, 797
BtsIMutI CAGTG 3 cut(s) 154, 244, 309
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 1 cut(s) 685
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 1 cut(s) 682
ClaI ATCGAT 1 cut(s) 783
CsiI ACCWGGT 1 cut(s) 678
CspAI ACCGGT 1 cut(s) 498
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 1 cut(s) 74
DdeI CTNAG 5 cut(s) 204, 312, 504, 521, 686
Ecl136II GAGCTC 1 cut(s) 795
Eco130I CCWWGG 2 cut(s) 210, 576
Eco24I GRGCYC 1 cut(s) 797
Eco47I GGWCC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 795
Eco57I CTGAAG 2 cut(s) 389, 666
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 2 cut(s) 49, 790
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 2 cut(s) 210, 576
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 2 cut(s) 210, 576
FaeI CATG 1 cut(s) 77
FaiI YATR 4 cut(s) 75, 444, 467, 652
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 751, 783
FatI CATG 1 cut(s) 73
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 7 cut(s) 109, 114, 117, 200, 342, 450, 743
FokI GGATG 6 cut(s) 389, 392, 466, 470, 680, 784
FriOI GRGCYC 1 cut(s) 797
Fsp4HI GCNGC 7 cut(s) 109, 114, 117, 200, 342, 450, 743
FspBI CTAG 1 cut(s) 383
GlaI GCGC 1 cut(s) 112
GluI GCNGC 7 cut(s) 109, 114, 117, 200, 342, 450, 743
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 2 cut(s) 209, 713
HapII CCGG 3 cut(s) 176, 499, 623
HhaI GCGC 1 cut(s) 113
Hin1II CATG 1 cut(s) 77
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HinfI GANTC 5 cut(s) 232, 267, 386, 536, 635
HpaII CCGG 3 cut(s) 176, 499, 623
HphI GGTGA 3 cut(s) 601, 668, 708
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 4 cut(s) 43, 505, 517, 640
Hpy188III TCNNGA 3 cut(s) 49, 685, 804
Hpy8I GTNNAC 1 cut(s) 676
HpyAV CCTTC 3 cut(s) 367, 469, 753
HpyCH4III ACNGT 3 cut(s) 14, 149, 239
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 4 cut(s) 275, 309, 341, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 569
HpyF3I CTNAG 5 cut(s) 204, 312, 504, 521, 686
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 77
HspAI GCGC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 178, 620
Lsp1109I GCAGC 4 cut(s) 100, 186, 353, 729
LweI GCATC 5 cut(s) 367, 406, 488, 631, 716
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 78
MboII GAAGA 4 cut(s) 21, 241, 328, 382
MhlI GDGCHC 2 cut(s) 306, 797
MluCI AATT 1 cut(s) 552
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 21
MseI TTAA 4 cut(s) 18, 69, 168, 548
MslI CAYNNNNRTG 2 cut(s) 470, 802
MspI CCGG 3 cut(s) 176, 499, 623
MspR9I CCNGG 2 cut(s) 624, 680
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 221, 569
NciI CCSGG 1 cut(s) 624
NlaIII CATG 1 cut(s) 77
NlaIV GGNNCC 3 cut(s) 174, 303, 463
NmeAIII GCCGAG 1 cut(s) 771
PaeR7I CTCGAG 2 cut(s) 49, 790
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 780
PfeI GAWTC 4 cut(s) 232, 267, 386, 635
PinAI ACCGGT 1 cut(s) 498
PkrI GCNGC 7 cut(s) 110, 115, 118, 201, 343, 451, 744
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 682
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 3 cut(s) 174, 303, 463
PspPI GGNCC 1 cut(s) 682
PspPPI RGGWCCY 1 cut(s) 682
PspXI VCTCGAGB 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 685
RseI CAYNNNNRTG 2 cut(s) 470, 802
SacI GAGCTC 1 cut(s) 797
SaqAI TTAA 4 cut(s) 18, 69, 168, 548
SatI GCNGC 7 cut(s) 109, 114, 117, 200, 342, 450, 743
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 624, 680
SduI GDGCHC 2 cut(s) 306, 797
SexAI ACCWGGT 1 cut(s) 678
SfaNI GCATC 5 cut(s) 367, 406, 488, 631, 716
Sfr274I CTCGAG 2 cut(s) 49, 790
SinI GGWCC 1 cut(s) 682
SlaI CTCGAG 2 cut(s) 49, 790
SmiMI CAYNNNNRTG 2 cut(s) 470, 802
SmlI CTYRAG 2 cut(s) 49, 790
SmoI CTYRAG 2 cut(s) 49, 790
Sse9I AATT 1 cut(s) 552
SsiI CCGC 5 cut(s) 93, 108, 116, 294, 449
SspMI CTAG 1 cut(s) 383
SstI GAGCTC 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 622, 678
StyI CCWWGG 2 cut(s) 210, 576
TaaI ACNGT 3 cut(s) 14, 149, 239
TaiI ACGT 1 cut(s) 81
TaqI TCGA 8 cut(s) 50, 60, 435, 534, 699, 774, 783, 791
TasI AATT 1 cut(s) 552
TauI GCSGC 3 cut(s) 111, 119, 452
TfiI GAWTC 4 cut(s) 232, 267, 386, 635
Tru1I TTAA 4 cut(s) 18, 69, 168, 548
Tru9I TTAA 4 cut(s) 18, 69, 168, 548
TscAI CASTG 3 cut(s) 154, 244, 316
TseI GCWGC 4 cut(s) 113, 199, 341, 742
TspDTI ATGAA 2 cut(s) 21, 438
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 3 cut(s) 154, 244, 316
VpaK11BI GGWCC 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XhoI CTCGAG 2 cut(s) 49, 790
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.