Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006777

Physical Location & Seq

Genomic Coordinates Forward (+)

44445204 .. 44450896

Gene Structure 5693 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1314300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGCCTCAGGTGAGGATCATCGCGAAGAATTTCATGGACATGGTGGCCGCCCTGCCCGCCATGAAGCTCGACAAGCTATACGAGAATGCATTCATCTGCGAAGCAATTCTCAGGTCTCTGCCACCGTTGGCTAAGAAATATGTCTTGCAACTGTTGTGCATTGAAGTTCCGGTGACTGCCAAGTCAATGGAGGAGTGGGTGCTTCCTGATGGGGTCTCGAAGCATAGAGTTGCAATTGATCGGCTGATTCAATTGAGAATATTTACTGAAACTGTGGACAGGAAGAGGGAAACAACCTACACACTAAATCCTATATTTCAGACTAATCTCAAGAAGCTTCTGGTACACGGTGTAGTTTTGCCGAGAGAACCAATGCCTTCAAATATCACAGTGAGGCTCCCAAGCTTGGAGGATCTTCAGGCTTTTGCCCTAGAACAATGGGAGTGTTTCTTGCTGCAACTTATCAACTCATCTCAAAGTGAGAGGCCATCGAACATCAGTTCGTCTATGATGAAAACTTTCCAGCGGGGTCTTTTGAGTCAAAGGGATAGAGAAGCTCCAAGATTAACTGACAGTGGTTTCCAGTTCTTGTTGATGGATACAAACGCGCAACTCTGGTACATCATCAGAGAGTACATCTCTAACTCGGAGGAGCGTGGGCTAGATTCTGCAGATTTGATTTCTTTCCTGCTAGAACTTAGTTTTCATGTTACAGGCGAGGCATATAACATAAATACATTGAATGAGAGGCAGAAGAATATAATCAAGGATCTTGCAGATTTGGGACTGATCAAACTTCAGCAGGGAAGGAAAGACAGTTGGTTTATACCTACTGAATTAGCTACTAATCTATCAGTTAGCTTGACAGACTCATCATCGAGGAAACAGGGATTTGTTGTTGTGGAAACAAACTTCAGACTCTATGCCTACTCAACATCTAAATTACATTGTGAAATTTTACGTCTTTTCTCAAGGGTAGAATATCAACTTCCAAACCTTATCGTTGGAGCAATAACAAAAGAAAGTTTATATAGTGCTTTTGAAAATGGCATCACTGGAGAACAGATAATTTCTTTTCTTCAGCAGAATGCACATCCACGCGTTGCAGAGAGAGTACCATCCGTACCTGAGAATGTCACAGATCAGATTAGGCTGTGGGAAACAGATCTGAATAGGGTTGAGATGACTCCAGCTTATTATTACGATGAATTTCCAACGAGGGAACTCCTCGAGGGTGCGTCTGACTTTGCGCGAGGCTATAATGCTCTGCTGTGGGAGGATGTAAATTCGAAAAAGATGCGTTTAGTGGTCAAGGCAGAAAACCACGTGCACATGCGAGAATATCTTGGACGTCAAAGGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000438 GO:0000439 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016310 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070816 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 ko05203 map03022 map03420 map05203

Pfam Domains

Protein Families

Tfb2

Protein Analysis

455

Amino Acids

52.36

Weight (kDa)

6.24

Isoelectric Point (pI)

41.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tfb2	PF03849	14 - 366	8.6e-114	Transcription factor Tfb2
Tfb2_C	PF18307	381 - 449	5.7e-19	Transcription factor Tfb2 (p52) C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17020 AT4G17020 AT4G17020
fragaria_vesca	FvH4_3g09430
malus_domestica	MD05G1314300.v1.1 MD10G1295600.v1.1
prunus_persica	Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1 Prupe.4G044800_v2.0.a1
pyrus_communis	pycom05g29230 pycom10g24830
rosa_chinensis	RchiOBHm_Chr5g0008121
rosa_laevigata	RLG00000031537
rosa_multiflora	Rmu_sc0001626.1_g000005
rosa_roxburghii	Rroxscaffold_1G00068370
rosa_rugosa	Rorug04G0433400
rosa_samantha	Rh5AG061600 Rh5BG058300 Rh5CG068500 Rh5DG057600
rosa_wichuraiana	Rw5G005900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	181
AatII	GACGTC	1 cut(s)	1354
AbsI	CCTCGAGG	1 cut(s)	1229
AccII	CGCG	4 cut(s)	23, 608, 1101, 1252
AciI	CCGC	3 cut(s)	48, 57, 526
AclWI	GGATC	3 cut(s)	23, 420, 777
AcoI	YGGCCR	1 cut(s)	45
AcsI	RAATTY	4 cut(s)	28, 954, 1208, 1285
AcuI	CTGAAG	4 cut(s)	401, 782, 898, 1064
AcvI	CACGTG	1 cut(s)	1327
AcyI	GRCGYC	1 cut(s)	1351
AfaI	GTAC	5 cut(s)	345, 620, 635, 1116, 1125
AfiI	CCNNNNNNNGG	1 cut(s)	406
AflIII	ACRYGT	1 cut(s)	1099
AgsI	TTSAA	5 cut(s)	164, 251, 381, 742, 1043
AluBI	AGCT	8 cut(s)	67, 76, 337, 405, 557, 842, 861, 1193
AluI	AGCT	8 cut(s)	67, 76, 337, 405, 557, 842, 861, 1193
Alw21I	GWGCWC	1 cut(s)	1332
Alw26I	GTCTC	2 cut(s)	120, 220
Alw44I	GTGCAC	1 cut(s)	1328
AlwI	GGATC	3 cut(s)	23, 420, 777
Ama87I	CYCGRG	1 cut(s)	1229
AoxI	GGCC	2 cut(s)	45, 485
ApaLI	GTGCAC	1 cut(s)	1328
ApeKI	GCWGC	1 cut(s)	454
ApoI	RAATTY	4 cut(s)	28, 954, 1208, 1285
Asp700I	GAANNNNTTC	4 cut(s)	29, 89, 105, 518
AspLEI	GCGC	2 cut(s)	610, 1252
AsuHPI	GGTGA	2 cut(s)	22, 184
AsuII	TTCGAA	1 cut(s)	1289
AvaI	CYCGRG	1 cut(s)	1229
AxyI	CCTNAGG	1 cut(s)	6
BaeGI	GKGCMC	1 cut(s)	1332
BbrPI	CACGTG	1 cut(s)	1327
Bbv12I	GWGCWC	1 cut(s)	1332
BbvI	GCAGC	1 cut(s)	441
BccI	CCATC	4 cut(s)	203, 496, 589, 1126
BcgI	CGANNNNNNTGC	2 cut(s)	1279, 1313
BciVI	GTATCC	1 cut(s)	592
BclI	TGATCA	1 cut(s)	789
BcoDI	GTCTC	2 cut(s)	120, 220
BfaI	CTAG	3 cut(s)	431, 662, 692
BfmI	CTRYAG	1 cut(s)	669
BfuI	GTATCC	1 cut(s)	592
BglII	AGATCT	1 cut(s)	1165
BisI	GCNGC	2 cut(s)	48, 455
BlsI	GCNGC	2 cut(s)	49, 456
BmeT110I	CYCGRG	1 cut(s)	1229
BmiI	GGNNCC	1 cut(s)	398
BmsI	GCATC	2 cut(s)	1059, 1287
BplI	GAGNNNNNCTC	2 cut(s)	623, 655
BpmI	CTGGAG	2 cut(s)	1077, 1173
Bpu14I	TTCGAA	1 cut(s)	1289
BpuEI	CTTGAG	2 cut(s)	314, 955
BsaAI	YACGTR	1 cut(s)	1327
BsaHI	GRCGYC	1 cut(s)	1351
BsaI	GGTCTC	2 cut(s)	120, 220
BsaWI	WCCGGW	1 cut(s)	169
Bsc4I	CCNNNNNNNGG	1 cut(s)	406
Bse1I	ACTGG	2 cut(s)	583, 1060
Bse21I	CCTNAGG	1 cut(s)	6
BseGI	GGATG	3 cut(s)	1093, 1118, 1285
BseLI	CCNNNNNNNGG	1 cut(s)	406
BseMII	CTCAG	3 cut(s)	20, 124, 1119
BseNI	ACTGG	2 cut(s)	583, 1060
BseRI	GAGGAG	3 cut(s)	206, 665, 1217
BseSI	GKGCMC	1 cut(s)	1332
BseXI	GCAGC	1 cut(s)	441
Bsh1236I	CGCG	4 cut(s)	23, 608, 1101, 1252
BshFI	GGCC	2 cut(s)	47, 487
BsiHKAI	GWGCWC	1 cut(s)	1332
BsiHKCI	CYCGRG	1 cut(s)	1229
BsiSI	CCGG	1 cut(s)	170
BslFI	GGGAC	1 cut(s)	798
BslI	CCNNNNNNNGG	1 cut(s)	406
BsmAI	GTCTC	2 cut(s)	120, 220
BsmFI	GGGAC	1 cut(s)	798
BsmI	GAATGC	3 cut(s)	89, 91, 1093
BsnI	GGCC	2 cut(s)	47, 487
Bso31I	GGTCTC	2 cut(s)	120, 220
BsoBI	CYCGRG	1 cut(s)	1229
Bsp119I	TTCGAA	1 cut(s)	1289
Bsp1286I	GDGCHC	1 cut(s)	1332
Bsp143I	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
Bsp68I	TCGCGA	1 cut(s)	23
BspACI	CCGC	3 cut(s)	48, 57, 526
BspANI	GGCC	2 cut(s)	47, 487
BspCNI	CTCAG	3 cut(s)	19, 123, 1120
BspFNI	CGCG	4 cut(s)	23, 608, 1101, 1252
BspLI	GGNNCC	1 cut(s)	398
BspMAI	CTGCAG	1 cut(s)	673
BspPI	GGATC	3 cut(s)	23, 420, 777
BspT104I	TTCGAA	1 cut(s)	1289
BspTNI	GGTCTC	2 cut(s)	120, 220
BsrI	ACTGG	2 cut(s)	583, 1060
BssMI	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
BssNI	GRCGYC	1 cut(s)	1351
Bst4CI	ACNGT	7 cut(s)	126, 153, 274, 350, 391, 575, 818
Bst6I	CTCTTC	1 cut(s)	278
BstACI	GRCGYC	1 cut(s)	1351
BstBAI	YACGTR	1 cut(s)	1327
BstBI	TTCGAA	1 cut(s)	1289
BstC8I	GCNNGC	1 cut(s)	57
BstDEI	CTNAG	5 cut(s)	6, 110, 132, 698, 1128
BstF5I	GGATG	3 cut(s)	1093, 1118, 1285
BstFNI	CGCG	4 cut(s)	23, 608, 1101, 1252
BstHHI	GCGC	2 cut(s)	610, 1252
BstKTI	GATC	7 cut(s)	18, 241, 415, 772, 792, 1144, 1168
BstMAI	GTCTC	2 cut(s)	120, 220
BstMBI	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 73
BstNSI	RCATGY	1 cut(s)	1336
BstSFI	CTRYAG	1 cut(s)	669
BstSLI	GKGCMC	1 cut(s)	1332
BstUI	CGCG	4 cut(s)	23, 608, 1101, 1252
BstV1I	GCAGC	1 cut(s)	441
BstX2I	RGATCY	3 cut(s)	412, 769, 1165
BstXI	CCANNNNNNTGG	1 cut(s)	187
BstYI	RGATCY	3 cut(s)	412, 769, 1165
Bsu36I	CCTNAGG	1 cut(s)	6
BsuI	GTATCC	1 cut(s)	592
BsuRI	GGCC	2 cut(s)	47, 487
BtgZI	GCGATG	1 cut(s)	4
BtsCI	GGATG	3 cut(s)	1093, 1118, 1285
BtsIMutI	CAGTG	3 cut(s)	396, 580, 1053
BtuMI	TCGCGA	1 cut(s)	23
Cac8I	GCNNGC	1 cut(s)	57
CfoI	GCGC	2 cut(s)	610, 1252
CseI	GACGC	1 cut(s)	1227
Csp6I	GTAC	5 cut(s)	344, 619, 634, 1115, 1124
CviAII	CATG	5 cut(s)	34, 40, 61, 707, 1333
CviQI	GTAC	5 cut(s)	344, 619, 634, 1115, 1124
DdeI	CTNAG	5 cut(s)	6, 110, 132, 698, 1128
DpnI	GATC	7 cut(s)	17, 240, 414, 771, 791, 1143, 1167
DpnII	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
DrdI	GACNNNNNNGTC	1 cut(s)	181
DseDI	GACNNNNNNGTC	1 cut(s)	181
EaeI	YGGCCR	1 cut(s)	45
Eam1104I	CTCTTC	1 cut(s)	278
EarI	CTCTTC	1 cut(s)	278
Eco31I	GGTCTC	2 cut(s)	120, 220
Eco57I	CTGAAG	4 cut(s)	401, 782, 898, 1064
Eco72I	CACGTG	1 cut(s)	1327
Eco81I	CCTNAGG	1 cut(s)	6
Eco88I	CYCGRG	1 cut(s)	1229
EcoT22I	ATGCAT	1 cut(s)	91
FaeI	CATG	5 cut(s)	37, 43, 64, 710, 1336
FaqI	GGGAC	1 cut(s)	798
FatI	CATG	5 cut(s)	33, 39, 60, 706, 1332
FauI	CCCGC	2 cut(s)	64, 519
FbaI	TGATCA	1 cut(s)	789
Fnu4HI	GCNGC	2 cut(s)	48, 455
FokI	GGATG	3 cut(s)	1080, 1105, 1292
Fsp4HI	GCNGC	2 cut(s)	48, 455
FspBI	CTAG	3 cut(s)	431, 662, 692
GlaI	GCGC	2 cut(s)	609, 1251
GluI	GCNGC	2 cut(s)	48, 455
GsuI	CTGGAG	2 cut(s)	1077, 1173
HaeIII	GGCC	2 cut(s)	47, 487
HapII	CCGG	1 cut(s)	170
HgaI	GACGC	1 cut(s)	1227
HhaI	GCGC	2 cut(s)	610, 1252
Hin1I	GRCGYC	1 cut(s)	1351
Hin1II	CATG	5 cut(s)	37, 43, 64, 710, 1336
Hin6I	GCGC	2 cut(s)	608, 1250
HinP1I	GCGC	2 cut(s)	608, 1250
HindIII	AAGCTT	2 cut(s)	335, 403
HinfI	GANTC	6 cut(s)	247, 538, 665, 869, 918, 1186
HpaII	CCGG	1 cut(s)	170
HphI	GGTGA	2 cut(s)	22, 184
Hpy166II	GTNNAC	3 cut(s)	277, 346, 1330
Hpy188I	TCNGA	7 cut(s)	321, 629, 649, 917, 1146, 1170, 1243
Hpy188III	TCNNGA	4 cut(s)	22, 206, 217, 331
Hpy8I	GTNNAC	3 cut(s)	277, 346, 1330
HpyAV	CCTTC	2 cut(s)	387, 801
HpyCH4III	ACNGT	7 cut(s)	126, 153, 274, 350, 391, 575, 818
HpyCH4IV	ACGT	3 cut(s)	961, 1326, 1351
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 73
HpyF3I	CTNAG	5 cut(s)	6, 110, 132, 698, 1128
HpySE526I	ACGT	3 cut(s)	961, 1326, 1351
Hsp92I	GRCGYC	1 cut(s)	1351
Hsp92II	CATG	5 cut(s)	37, 43, 64, 710, 1336
HspAI	GCGC	2 cut(s)	608, 1250
Ksp22I	TGATCA	1 cut(s)	789
Kzo9I	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
LmnI	GCTCC	4 cut(s)	402, 562, 652, 1007
Lsp1109I	GCAGC	1 cut(s)	441
LweI	GCATC	2 cut(s)	1059, 1287
MaeI	CTAG	3 cut(s)	431, 662, 692
MaeII	ACGT	3 cut(s)	961, 1326, 1351
MaeIII	GTNAC	3 cut(s)	172, 709, 1135
MalI	GATC	7 cut(s)	17, 240, 414, 771, 791, 1143, 1167
MboI	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
MboII	GAAGA	5 cut(s)	37, 295, 407, 766, 1070
MfeI	CAATTG	2 cut(s)	234, 251
MflI	RGATCY	3 cut(s)	412, 769, 1165
MhlI	GDGCHC	1 cut(s)	1332
MluI	ACGCGT	1 cut(s)	1099
MlyI	GAGTC	4 cut(s)	547, 863, 912, 1180
MmeI	TCCRAC	2 cut(s)	985, 1238
Mph1103I	ATGCAT	1 cut(s)	91
MroXI	GAANNNNTTC	4 cut(s)	29, 89, 105, 518
MseI	TTAA	1 cut(s)	566
MslI	CAYNNNNRTG	1 cut(s)	38
MspA1I	CMGCKG	1 cut(s)	526
MspI	CCGG	1 cut(s)	170
MunI	CAATTG	2 cut(s)	234, 251
Mva1269I	GAATGC	3 cut(s)	89, 91, 1093
MvnI	CGCG	4 cut(s)	23, 608, 1101, 1252
MwoI	GCNNNNNNNGC	2 cut(s)	56, 73
NdeII	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
NlaIII	CATG	5 cut(s)	37, 43, 64, 710, 1336
NlaIV	GGNNCC	1 cut(s)	398
NmeAIII	GCCGAG	1 cut(s)	387
NmuCI	GTSAC	2 cut(s)	172, 1135
NruI	TCGCGA	1 cut(s)	23
NsiI	ATGCAT	1 cut(s)	91
NspI	RCATGY	1 cut(s)	1336
NspV	TTCGAA	1 cut(s)	1289
PaeR7I	CTCGAG	1 cut(s)	1229
PctI	GAATGC	3 cut(s)	89, 91, 1093
PdmI	GAANNNNTTC	4 cut(s)	29, 89, 105, 518
PfeI	GAWTC	2 cut(s)	247, 665
PkrI	GCNGC	2 cut(s)	49, 456
PleI	GAGTC	4 cut(s)	546, 863, 912, 1180
PmaCI	CACGTG	1 cut(s)	1327
PmlI	CACGTG	1 cut(s)	1327
PpsI	GAGTC	4 cut(s)	546, 863, 912, 1180
Ppu21I	YACGTR	1 cut(s)	1327
PspCI	CACGTG	1 cut(s)	1327
PspN4I	GGNNCC	1 cut(s)	398
PspXI	VCTCGAGB	1 cut(s)	1229
PstI	CTGCAG	1 cut(s)	673
PsuI	RGATCY	3 cut(s)	412, 769, 1165
RruI	TCGCGA	1 cut(s)	23
RsaI	GTAC	5 cut(s)	345, 620, 635, 1116, 1125
RsaNI	GTAC	5 cut(s)	344, 619, 634, 1115, 1124
RseI	CAYNNNNRTG	1 cut(s)	38
SaqAI	TTAA	1 cut(s)	566
SatI	GCNGC	2 cut(s)	48, 455
Sau3AI	GATC	7 cut(s)	15, 238, 412, 769, 789, 1141, 1165
SchI	GAGTC	4 cut(s)	547, 863, 912, 1180
SduI	GDGCHC	1 cut(s)	1332
SfaNI	GCATC	2 cut(s)	1059, 1287
SfcI	CTRYAG	1 cut(s)	669
Sfr274I	CTCGAG	1 cut(s)	1229
SfuI	TTCGAA	1 cut(s)	1289
SlaI	CTCGAG	1 cut(s)	1229
SmiMI	CAYNNNNRTG	1 cut(s)	38
SmlI	CTYRAG	3 cut(s)	329, 970, 1229
SmoI	CTYRAG	3 cut(s)	329, 970, 1229
SsiI	CCGC	3 cut(s)	48, 57, 526
SspI	AATATT	1 cut(s)	261
SspMI	CTAG	3 cut(s)	431, 662, 692
TaaI	ACNGT	7 cut(s)	126, 153, 274, 350, 391, 575, 818
TaiI	ACGT	3 cut(s)	964, 1329, 1354
TaqI	TCGA	6 cut(s)	69, 218, 491, 878, 1230, 1289
TatI	WGTACW	1 cut(s)	633
TauI	GCSGC	1 cut(s)	50
TfiI	GAWTC	2 cut(s)	247, 665
Tru1I	TTAA	1 cut(s)	566
Tru9I	TTAA	1 cut(s)	566
TscAI	CASTG	3 cut(s)	396, 580, 1060
TseFI	GTSAC	2 cut(s)	172, 1135
TseI	GCWGC	1 cut(s)	454
Tsp45I	GTSAC	2 cut(s)	172, 1135
TspDTI	ATGAA	6 cut(s)	22, 77, 82, 527, 695, 1221
TspGWI	ACGGA	1 cut(s)	1111
TspRI	CASTG	3 cut(s)	396, 580, 1060
VneI	GTGCAC	1 cut(s)	1328
XapI	RAATTY	4 cut(s)	28, 954, 1208, 1285
XceI	RCATGY	1 cut(s)	1336
XhoI	CTCGAG	1 cut(s)	1229
XmnI	GAANNNNTTC	4 cut(s)	29, 89, 105, 518
XspI	CTAG	3 cut(s)	431, 662, 692
ZraI	GACGTC	1 cut(s)	1352
Zsp2I	ATGCAT	1 cut(s)	91

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.