MD05G1314500.v1.1

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014685
Physical Location & Seq
Forward (+)
44451757 .. 44454049
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1314500.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCGGCAACAATTGCAAGAATCTGCCTGCAGTCACTGCTGAAAGCTATGAACTCACTTTTGGGAATGGTTGGAATTGCAATGATTATGTATGGCCTTTGGATGCTTAGAGTTTGGCTGAGGGACTCTGACGGTTCGCCCCTTGACCATCATAGTGCTGTTCCTCCATGGTTTATATGCACTTCTCTTGGCGCCGGTTTTACCGTGTGCATAATAACATGTATAGGTCACGTTGCTGCAAAGTTTACGCATGGCTGTTGCCTCTCTTTTTATATGTTGATCATGTTTTTGCTGCTTCTATTGGAGTTTGCAGCGGCTGCAGATATACTTTTAAATTCAGACTGGGAAAAGGATTTACCATATGACCGGACAGGAAAGTTCAGTGATTTCAAAGATTTTGTCGAGTCGAAATTGGACATATTTCAGTGGATAGGCTTGTTCATCGTCTTAGCACAGGGTTTATCAATCGTACTAGCCACAGCTCTAAGGTCTGCAGGGCCAAACCAAAGATCCAGCTACGATAGCGATGAAGAGTGTCCCCCGGAGAGACTCCCGCTCATAAATCCTAATGGTCAACCTGCCAAGAACAGTAACTGGAATTCAATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

204

Amino Acids

22.51

Weight (kDa)

5.75

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 13 - 115 3.1e-11 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 191
AccBSI CCGCTC 1 cut(s) 556
AciI CCGC 3 cut(s) 5, 314, 554
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 2 cut(s) 334, 598
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 471
AflIII ACRYGT 1 cut(s) 218
AgsI TTSAA 2 cut(s) 391, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 3 cut(s) 47, 482, 516
AluI AGCT 3 cut(s) 47, 482, 516
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 1 cut(s) 504
AlwNI CAGNNNCTG 3 cut(s) 37, 317, 594
AoxI GGCC 2 cut(s) 94, 497
ApeKI GCWGC 4 cut(s) 236, 292, 311, 317
ApoI RAATTY 2 cut(s) 334, 598
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 542
BanI GGYRCC 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 4 cut(s) 223, 279, 304, 323
BccI CCATC 1 cut(s) 156
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 541
BfaI CTAG 1 cut(s) 473
BfmI CTRYAG 3 cut(s) 29, 318, 492
BfoI RGCGCY 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 586
BisI GCNGC 6 cut(s) 6, 237, 293, 312, 315, 318
BlsI GCNGC 6 cut(s) 7, 238, 294, 313, 316, 319
Bme1390I CCNGG 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 352
Bpu10I CCTNAGC 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 167, 540
BsaWI WCCGGW 1 cut(s) 366
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 347, 599
Bse3DI GCAATG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 167, 540
BseGI GGATG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 2 cut(s) 347, 599
BseXI GCAGC 4 cut(s) 223, 279, 304, 323
BshFI GGCC 2 cut(s) 96, 499
BshNI GGYRCC 1 cut(s) 191
BsiSI CCGG 3 cut(s) 195, 367, 542
BslFI GGGAC 2 cut(s) 137, 522
BsmAI GTCTC 1 cut(s) 541
BsmFI GGGAC 2 cut(s) 137, 522
BsnI GGCC 2 cut(s) 96, 499
Bsp143I GATC 2 cut(s) 279, 509
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 3 cut(s) 5, 314, 554
BspANI GGCC 2 cut(s) 96, 499
BspCNI CTCAG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 3 cut(s) 33, 322, 496
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 2 cut(s) 347, 599
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 167, 540
BssMI GATC 2 cut(s) 279, 509
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 167
Bst4CI ACNGT 3 cut(s) 134, 205, 590
Bst6I CTCTTC 1 cut(s) 525
BstACI GRCGYC 1 cut(s) 192
BstAPI GCANNNNNTGC 3 cut(s) 14, 37, 317
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 4 cut(s) 107, 119, 448, 485
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 2 cut(s) 282, 512
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 2 cut(s) 279, 509
BstMWI GCNNNNNNNGC 4 cut(s) 14, 37, 317, 522
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 3 cut(s) 29, 318, 492
BstV1I GCAGC 4 cut(s) 223, 279, 304, 323
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BsuRI GGCC 2 cut(s) 96, 499
BtgI CCRYGG 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 3 cut(s) 35, 388, 431
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 1 cut(s) 29
CaiI CAGNNNCTG 3 cut(s) 37, 317, 594
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 1 cut(s) 470
CviAII CATG 4 cut(s) 168, 219, 251, 283
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 4 cut(s) 107, 119, 448, 485
DinI GGCGCC 1 cut(s) 193
DpnI GATC 2 cut(s) 281, 511
DpnII GATC 2 cut(s) 279, 509
DraI TTTAAA 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 167
EgeI GGCGCC 1 cut(s) 193
EheI GGCGCC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 4 cut(s) 171, 222, 254, 286
FaqI GGGAC 2 cut(s) 137, 522
FatI CATG 4 cut(s) 167, 218, 250, 282
FauI CCCGC 1 cut(s) 561
FauNDI CATATG 1 cut(s) 361
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 6, 237, 293, 312, 315, 318
FokI GGATG 1 cut(s) 115
Fsp4HI GCNGC 6 cut(s) 6, 237, 293, 312, 315, 318
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 193
GluI GCNGC 6 cut(s) 6, 237, 293, 312, 315, 318
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 2 cut(s) 96, 499
HapII CCGG 3 cut(s) 195, 367, 542
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 171, 222, 254, 286
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 575
HindII GTYRAC 1 cut(s) 575
HinfI GANTC 4 cut(s) 21, 125, 404, 549
HpaII CCGG 3 cut(s) 195, 367, 542
Hpy166II GTNNAC 2 cut(s) 246, 575
Hpy188I TCNGA 2 cut(s) 130, 340
Hpy8I GTNNAC 2 cut(s) 246, 575
HpyCH4III ACNGT 3 cut(s) 134, 205, 590
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 9 cut(s) 17, 31, 80, 180, 210, 239, 311, 320, 494
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 37, 317, 522
HpyF3I CTNAG 4 cut(s) 107, 119, 448, 485
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 171, 222, 254, 286
HspAI GCGC 1 cut(s) 192
KasI GGCGCC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 2 cut(s) 279, 509
Lsp1109I GCAGC 4 cut(s) 223, 279, 304, 323
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 33, 227, 590
MalI GATC 2 cut(s) 281, 511
MbiI CCGCTC 1 cut(s) 556
MboI GATC 2 cut(s) 279, 509
MboII GAAGA 1 cut(s) 542
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 509
MluCI AATT 5 cut(s) 12, 75, 334, 410, 598
Mly113I GGCGCC 1 cut(s) 192
MlyI GAGTC 3 cut(s) 119, 413, 543
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 3 cut(s) 114, 174, 272
MseI TTAA 1 cut(s) 332
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 3 cut(s) 195, 367, 542
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 1 cut(s) 12
MwoI GCNNNNNNNGC 4 cut(s) 14, 37, 317, 522
NarI GGCGCC 1 cut(s) 192
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 1 cut(s) 167
NdeI CATATG 1 cut(s) 361
NdeII GATC 2 cut(s) 279, 509
NlaIII CATG 4 cut(s) 171, 222, 254, 286
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 2 cut(s) 33, 227
NspI RCATGY 1 cut(s) 222
PciI ACATGT 1 cut(s) 218
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 6 cut(s) 7, 238, 294, 313, 316, 319
PleI GAGTC 3 cut(s) 119, 412, 543
PluTI GGCGCC 1 cut(s) 195
PpsI GAGTC 3 cut(s) 119, 412, 543
PscI ACATGT 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 1 cut(s) 497
PstI CTGCAG 3 cut(s) 33, 322, 496
PstNI CAGNNNCTG 3 cut(s) 37, 317, 594
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 1 cut(s) 332
SatI GCNGC 6 cut(s) 6, 237, 293, 312, 315, 318
Sau3AI GATC 2 cut(s) 279, 509
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 3 cut(s) 119, 413, 543
ScrFI CCNGG 1 cut(s) 542
SetI ASST 7 cut(s) 49, 229, 234, 484, 491, 518, 580
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 3 cut(s) 29, 318, 492
SfoI GGCGCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 153
Sse9I AATT 5 cut(s) 12, 75, 334, 410, 598
SsiI CCGC 3 cut(s) 5, 314, 554
SspDI GGCGCC 1 cut(s) 191
SspMI CTAG 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 3 cut(s) 134, 205, 590
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 402, 407
TasI AATT 5 cut(s) 12, 75, 334, 410, 598
TauI GCSGC 2 cut(s) 8, 317
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 332
Tru9I TTAA 1 cut(s) 332
TscAI CASTG 3 cut(s) 42, 388, 431
TseFI GTSAC 2 cut(s) 33, 227
TseI GCWGC 4 cut(s) 236, 292, 311, 317
Tsp45I GTSAC 2 cut(s) 33, 227
TspDTI ATGAA 3 cut(s) 65, 430, 543
TspRI CASTG 3 cut(s) 42, 388, 431
XapI RAATTY 2 cut(s) 334, 598
XceI RCATGY 1 cut(s) 222
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.