Rroxscaffold_1G00068380

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014685
Physical Location & Seq
Forward (+)
89584697 .. 89586090
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00068380.1

Sequence Viewer

Length: 675 bp
ATGGCGAAAATCGCAAGAGTCCTTCTGCAGTCACTGCTTAAAGCAATGAATTCGATTTTGGGACTGCTTGGAATTGCATTGATTCTCTATGGTCTTTGGATGGTTAGAGTTTGGGAGAGGGACATGGATGGCTCATCATCCTTTGATCAATATACTACTGCTCCATGGGTTATCTGCACTTTTATTGGGGCTGGTGTTACCTTGTGCATAATAACAGGCATAGGTCATCTTGCTGCAAACTGTGGCAGTGGTTGTTGCCTCTCTTGTTATGCGTTGATTATCTGTATGCTTCTTCTATTGGAGACTGCAATGGCTGCAGATATACTCTTAAATTCTGACTGGGAGAAGGATCTCCCCTATGACCCTACTGGAAAGTTCAATGATTTCAGAGATTTTGTGGAGTCAAACTTCGACATGTTCAAGTGGATAGGCTTGCTGATCATCTTAGCTCAGAAATGGTTTAGACCTGTTGACAAAGTTAATGATCTCCAGGGTTTTTCGATCTTATTGGCCACGGCTTTAAGGTCGATAGGGCCAGAGCAAGTACCAAACTGTGACAGTGATGAAGAGTATGCCCCAGAAAGAGTCCCACTCATGAACCATATCGTTCAGCCACCAGAATACATTGTTGGTGCCAAGAACAGCAGCAACTGGAATGTAAGAATTCACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

224

Amino Acids

24.97

Weight (kDa)

4.89

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 114 1.1e-11 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 632
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 3 cut(s) 49, 331, 663
AfaI GTAC 1 cut(s) 546
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 379, 421
AjnI CCWGG 1 cut(s) 489
AjuI GAANNNNNNNTTGG 4 cut(s) 41, 73, 612, 644
AluBI AGCT 1 cut(s) 449
AluI AGCT 1 cut(s) 449
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 2 cut(s) 34, 651
AoxI GGCC 2 cut(s) 510, 533
ApeKI GCWGC 3 cut(s) 233, 314, 645
ApoI RAATTY 3 cut(s) 49, 331, 663
AspS9I GGNCC 1 cut(s) 533
BalI TGGCCA 1 cut(s) 512
BanI GGYRCC 1 cut(s) 632
BbvI GCAGC 3 cut(s) 220, 301, 657
BccI CCATC 2 cut(s) 94, 122
BceAI ACGGC 1 cut(s) 531
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BciT130I CCWGG 1 cut(s) 491
BclI TGATCA 2 cut(s) 145, 438
BcoDI GTCTC 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 26, 315
BisI GCNGC 3 cut(s) 234, 315, 646
BlsI GCNGC 3 cut(s) 235, 316, 647
Bme1390I CCNGG 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 1 cut(s) 491
BmrI ACTGGG 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 576, 608
BpmI CTGGAG 1 cut(s) 473
BsaJI CCNNGG 3 cut(s) 164, 490, 513
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse1I ACTGG 3 cut(s) 344, 373, 656
Bse3DI GCAATG 2 cut(s) 51, 315
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 3 cut(s) 164, 490, 513
BseGI GGATG 3 cut(s) 105, 133, 137
BseMI GCAATG 2 cut(s) 51, 315
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 3 cut(s) 344, 373, 656
BseXI GCAGC 3 cut(s) 220, 301, 657
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 2 cut(s) 512, 535
BshNI GGYRCC 1 cut(s) 632
BslFI GGGAC 3 cut(s) 75, 134, 572
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 3 cut(s) 75, 134, 572
BsnI GGCC 2 cut(s) 512, 535
Bsp143I GATC 5 cut(s) 145, 349, 438, 484, 501
Bsp19I CCATGG 1 cut(s) 164
BspANI GGCC 2 cut(s) 512, 535
BspCNI CTCAG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 1 cut(s) 634
BspMAI CTGCAG 2 cut(s) 30, 319
BspPI GGATC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 632
BsrDI GCAATG 2 cut(s) 51, 315
BsrI ACTGG 3 cut(s) 344, 373, 656
BssECI CCNNGG 3 cut(s) 164, 490, 513
BssMI GATC 5 cut(s) 145, 349, 438, 484, 501
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 3 cut(s) 242, 554, 560
Bst6I CTCTTC 1 cut(s) 561
BstAPI GCANNNNNTGC 2 cut(s) 34, 314
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 445, 450
BstDSI CCRYGG 2 cut(s) 164, 513
BstF5I GGATG 3 cut(s) 105, 133, 137
BstKTI GATC 5 cut(s) 148, 352, 441, 487, 504
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 145, 349, 438, 484, 501
BstMWI GCNNNNNNNGC 3 cut(s) 11, 34, 314
BstNI CCWGG 1 cut(s) 491
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 2 cut(s) 26, 315
BstV1I GCAGC 3 cut(s) 220, 301, 657
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 2 cut(s) 512, 535
BtgI CCRYGG 2 cut(s) 164, 513
BtsCI GGATG 3 cut(s) 105, 133, 137
BtsI GCAGTG 2 cut(s) 32, 253
BtsIMutI CAGTG 3 cut(s) 32, 253, 565
Cac8I GCNNGC 1 cut(s) 434
CaiI CAGNNNCTG 2 cut(s) 34, 651
CciI TCATGA 1 cut(s) 594
Cfr13I GGNCC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 545
CviAII CATG 4 cut(s) 124, 165, 415, 595
CviJI RGCY 9 cut(s) 132, 191, 314, 432, 449, 512, 518, 535, 613
CviKI_1 RGCY 9 cut(s) 132, 191, 314, 432, 449, 512, 518, 535, 613
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 2 cut(s) 445, 450
DpnI GATC 5 cut(s) 147, 351, 440, 486, 503
DpnII GATC 5 cut(s) 145, 349, 438, 484, 501
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 164
EcoRI GAATTC 2 cut(s) 49, 663
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 4 cut(s) 127, 168, 418, 598
FaqI GGGAC 3 cut(s) 75, 134, 572
FatI CATG 4 cut(s) 123, 164, 414, 594
FbaI TGATCA 2 cut(s) 145, 438
Fnu4HI GCNGC 3 cut(s) 234, 315, 646
FokI GGATG 3 cut(s) 112, 124, 140
Fsp4HI GCNGC 3 cut(s) 234, 315, 646
GluI GCNGC 3 cut(s) 234, 315, 646
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 2 cut(s) 512, 535
Hin1II CATG 4 cut(s) 127, 168, 418, 598
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HinfI GANTC 4 cut(s) 18, 82, 401, 585
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 3 cut(s) 337, 389, 453
Hpy188III TCNNGA 1 cut(s) 595
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 32, 340
HpyCH4III ACNGT 3 cut(s) 242, 554, 560
HpyCH4V TGCA 7 cut(s) 28, 77, 177, 207, 236, 308, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 34, 314
HpyF3I CTNAG 2 cut(s) 445, 450
Hsp92II CATG 4 cut(s) 127, 168, 418, 598
Ksp22I TGATCA 2 cut(s) 145, 438
Kzo9I GATC 5 cut(s) 145, 349, 438, 484, 501
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 3 cut(s) 220, 301, 657
MaeIII GTNAC 3 cut(s) 30, 196, 554
MalI GATC 5 cut(s) 147, 351, 440, 486, 503
MboI GATC 5 cut(s) 145, 349, 438, 484, 501
MboII GAAGA 2 cut(s) 284, 578
MflI RGATCY 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 4 cut(s) 49, 72, 331, 663
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 3 cut(s) 27, 410, 594
MnlI CCTC 2 cut(s) 111, 269
Mox20I TGGCCA 1 cut(s) 512
MscI TGGCCA 1 cut(s) 512
MseI TTAA 4 cut(s) 39, 329, 480, 521
Msp20I TGGCCA 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 491
MwoI GCNNNNNNNGC 3 cut(s) 11, 34, 314
NcoI CCATGG 1 cut(s) 164
NdeII GATC 5 cut(s) 145, 349, 438, 484, 501
NlaIII CATG 4 cut(s) 127, 168, 418, 598
NlaIV GGNNCC 1 cut(s) 634
NmuCI GTSAC 2 cut(s) 30, 554
NspI RCATGY 1 cut(s) 418
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 414
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 235, 316, 647
PleI GAGTC 3 cut(s) 26, 409, 593
PpsI GAGTC 3 cut(s) 26, 409, 593
PscI ACATGT 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 489
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 1 cut(s) 533
PstI CTGCAG 2 cut(s) 30, 319
PstNI CAGNNNCTG 2 cut(s) 34, 651
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
SaqAI TTAA 4 cut(s) 39, 329, 480, 521
SatI GCNGC 3 cut(s) 234, 315, 646
Sau3AI GATC 5 cut(s) 145, 349, 438, 484, 501
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 3 cut(s) 27, 410, 594
ScrFI CCNGG 1 cut(s) 491
SetI ASST 5 cut(s) 203, 226, 451, 469, 527
SfcI CTRYAG 2 cut(s) 26, 315
Sse9I AATT 4 cut(s) 49, 72, 331, 663
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 3 cut(s) 242, 554, 560
TaqI TCGA 4 cut(s) 53, 411, 500, 527
TasI AATT 4 cut(s) 49, 72, 331, 663
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 4 cut(s) 39, 329, 480, 521
Tru9I TTAA 4 cut(s) 39, 329, 480, 521
TscAI CASTG 3 cut(s) 39, 253, 565
TseFI GTSAC 2 cut(s) 30, 554
TseI GCWGC 3 cut(s) 233, 314, 645
Tsp45I GTSAC 2 cut(s) 30, 554
TspDTI ATGAA 3 cut(s) 62, 579, 611
TspRI CASTG 3 cut(s) 39, 253, 565
XapI RAATTY 3 cut(s) 49, 331, 663
XceI RCATGY 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.