Rh5DG057400

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014685
Physical Location & Seq
Reverse (-)
4887179 .. 4891585
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG057400.1

Sequence Viewer

Length: 636 bp
ATGGCGAAAATCGCAAGAGTCCTTCTGCAGTCACTGCTTAAAGCAATGAATTCGATTTTGGGACTGCTTGGAATTGCATTGATTCTCTATGGTCTTTGGATGGTTAGAGTTTGGGAGAGGGACATGGATAGCTCATCATCCTTTGATCAATATACTACTGCTCCATGGGTTATCTGCACTTTTATTGGGGCTGGTGTTACCTTGTGCATAATAACAGGGATAGGTCATCTTGCTGCAAACTGTGGCAGTGGTTGTTGCCTGTCTTGTTATGCTTTGATTATCTGTATGCTTCTTCTATTGGAGACTGCAATGGCTGCAGATATACTCTTAAATTCTGACTGGGAGAAGGATCTCCCCTATGACCCTACTGGAAAGTTCAATGATTTCAGAGATTTTGTGGAGTCAAACTTCGACATGTTCAAGTGGATAGGCCTGCTGATCATCTTAGCTCAGGGTTTTTCGATCTTATTGGCCACGGCTTTAAGGTCGATAGGGCCAGAGCAAGTATCAAACTGTGACAGTGATGAAGAGTATGCCCCAACAAGAGTCCCACTCATGAACCATATCGTTCAGCCACCAGAATACATTGTTGGTGCCAAGAATAGCAGTAACTGGAATGTAAGAATTCACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

211

Amino Acids

23.33

Weight (kDa)

4.82

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 113 2.9e-11 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 593
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 3 cut(s) 49, 331, 624
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 379, 421
AjuI GAANNNNNNNTTGG 4 cut(s) 41, 73, 573, 605
AluBI AGCT 2 cut(s) 132, 449
AluI AGCT 2 cut(s) 132, 449
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 2 cut(s) 34, 612
AoxI GGCC 3 cut(s) 430, 471, 494
ApeKI GCWGC 2 cut(s) 233, 314
ApoI RAATTY 3 cut(s) 49, 331, 624
AspS9I GGNCC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 593
BbvI GCAGC 2 cut(s) 220, 301
BccI CCATC 1 cut(s) 94
BceAI ACGGC 1 cut(s) 492
BcgI CGANNNNNNTGC 2 cut(s) 33, 67
BclI TGATCA 2 cut(s) 145, 438
BcoDI GTCTC 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 26, 315
BisI GCNGC 2 cut(s) 234, 315
BlsI GCNGC 2 cut(s) 235, 316
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 595
BmrI ACTGGG 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 537, 569
Bpu10I CCTNAGC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 164, 474
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse1I ACTGG 3 cut(s) 344, 373, 617
Bse3DI GCAATG 2 cut(s) 51, 315
BseDI CCNNGG 2 cut(s) 164, 474
BseGI GGATG 2 cut(s) 105, 137
BseMI GCAATG 2 cut(s) 51, 315
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 3 cut(s) 344, 373, 617
BseXI GCAGC 2 cut(s) 220, 301
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 3 cut(s) 432, 473, 496
BshNI GGYRCC 1 cut(s) 593
BslFI GGGAC 3 cut(s) 75, 134, 533
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 3 cut(s) 75, 134, 533
BsnI GGCC 3 cut(s) 432, 473, 496
Bsp143I GATC 4 cut(s) 145, 349, 438, 462
Bsp19I CCATGG 1 cut(s) 164
BspANI GGCC 3 cut(s) 432, 473, 496
BspCNI CTCAG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 1 cut(s) 595
BspMAI CTGCAG 2 cut(s) 30, 319
BspPI GGATC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 593
BsrDI GCAATG 2 cut(s) 51, 315
BsrI ACTGG 3 cut(s) 344, 373, 617
BssECI CCNNGG 2 cut(s) 164, 474
BssMI GATC 4 cut(s) 145, 349, 438, 462
BssT1I CCWWGG 1 cut(s) 164
Bst4CI ACNGT 3 cut(s) 242, 515, 521
Bst6I CTCTTC 1 cut(s) 522
BstAPI GCANNNNNTGC 2 cut(s) 34, 314
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 445, 450
BstDSI CCRYGG 2 cut(s) 164, 474
BstF5I GGATG 2 cut(s) 105, 137
BstKTI GATC 4 cut(s) 148, 352, 441, 465
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 4 cut(s) 145, 349, 438, 462
BstMWI GCNNNNNNNGC 3 cut(s) 11, 34, 314
BstNSI RCATGY 1 cut(s) 418
BstSFI CTRYAG 2 cut(s) 26, 315
BstV1I GCAGC 2 cut(s) 220, 301
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 3 cut(s) 432, 473, 496
BtgI CCRYGG 2 cut(s) 164, 474
BtsCI GGATG 2 cut(s) 105, 137
BtsI GCAGTG 2 cut(s) 32, 253
BtsIMutI CAGTG 3 cut(s) 32, 253, 526
Cac8I GCNNGC 1 cut(s) 434
CaiI CAGNNNCTG 2 cut(s) 34, 612
CciI TCATGA 1 cut(s) 555
Cfr13I GGNCC 1 cut(s) 494
CviAII CATG 4 cut(s) 124, 165, 415, 556
CviJI RGCY 9 cut(s) 132, 191, 314, 432, 449, 473, 479, 496, 574
CviKI_1 RGCY 9 cut(s) 132, 191, 314, 432, 449, 473, 479, 496, 574
DdeI CTNAG 2 cut(s) 445, 450
DpnI GATC 4 cut(s) 147, 351, 440, 464
DpnII GATC 4 cut(s) 145, 349, 438, 462
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 432
EcoRI GAATTC 2 cut(s) 49, 624
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 4 cut(s) 127, 168, 418, 559
FaqI GGGAC 3 cut(s) 75, 134, 533
FatI CATG 4 cut(s) 123, 164, 414, 555
FbaI TGATCA 2 cut(s) 145, 438
Fnu4HI GCNGC 2 cut(s) 234, 315
FokI GGATG 2 cut(s) 112, 124
Fsp4HI GCNGC 2 cut(s) 234, 315
GluI GCNGC 2 cut(s) 234, 315
HaeIII GGCC 3 cut(s) 432, 473, 496
Hin1II CATG 4 cut(s) 127, 168, 418, 559
HinfI GANTC 4 cut(s) 18, 82, 401, 546
Hpy188I TCNGA 2 cut(s) 337, 389
Hpy188III TCNNGA 1 cut(s) 556
HpyAV CCTTC 2 cut(s) 32, 340
HpyCH4III ACNGT 3 cut(s) 242, 515, 521
HpyCH4V TGCA 7 cut(s) 28, 77, 177, 207, 236, 308, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 34, 314
HpyF3I CTNAG 2 cut(s) 445, 450
Hsp92II CATG 4 cut(s) 127, 168, 418, 559
Ksp22I TGATCA 2 cut(s) 145, 438
Kzo9I GATC 4 cut(s) 145, 349, 438, 462
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 2 cut(s) 220, 301
MaeIII GTNAC 4 cut(s) 30, 196, 515, 608
MalI GATC 4 cut(s) 147, 351, 440, 464
MboI GATC 4 cut(s) 145, 349, 438, 462
MboII GAAGA 2 cut(s) 284, 539
MflI RGATCY 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 4 cut(s) 49, 72, 331, 624
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 3 cut(s) 27, 410, 555
MnlI CCTC 1 cut(s) 111
Mox20I TGGCCA 1 cut(s) 473
MscI TGGCCA 1 cut(s) 473
MseI TTAA 3 cut(s) 39, 329, 482
Msp20I TGGCCA 1 cut(s) 473
MwoI GCNNNNNNNGC 3 cut(s) 11, 34, 314
NcoI CCATGG 1 cut(s) 164
NdeII GATC 4 cut(s) 145, 349, 438, 462
NlaIII CATG 4 cut(s) 127, 168, 418, 559
NlaIV GGNNCC 1 cut(s) 595
NmuCI GTSAC 2 cut(s) 30, 515
NspI RCATGY 1 cut(s) 418
PagI TCATGA 1 cut(s) 555
PceI AGGCCT 1 cut(s) 432
PciI ACATGT 1 cut(s) 414
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 235, 316
PleI GAGTC 3 cut(s) 26, 409, 554
PpsI GAGTC 3 cut(s) 26, 409, 554
PscI ACATGT 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 595
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 2 cut(s) 30, 319
PstNI CAGNNNCTG 2 cut(s) 34, 612
PsuI RGATCY 1 cut(s) 349
SaqAI TTAA 3 cut(s) 39, 329, 482
SatI GCNGC 2 cut(s) 234, 315
Sau3AI GATC 4 cut(s) 145, 349, 438, 462
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 3 cut(s) 27, 410, 555
SetI ASST 5 cut(s) 134, 203, 226, 451, 488
SfcI CTRYAG 2 cut(s) 26, 315
Sse9I AATT 4 cut(s) 49, 72, 331, 624
SseBI AGGCCT 1 cut(s) 432
StuI AGGCCT 1 cut(s) 432
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 3 cut(s) 242, 515, 521
TaqI TCGA 4 cut(s) 53, 411, 461, 488
TasI AATT 4 cut(s) 49, 72, 331, 624
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 3 cut(s) 39, 329, 482
Tru9I TTAA 3 cut(s) 39, 329, 482
TscAI CASTG 3 cut(s) 39, 253, 526
TseFI GTSAC 2 cut(s) 30, 515
TseI GCWGC 2 cut(s) 233, 314
Tsp45I GTSAC 2 cut(s) 30, 515
TspDTI ATGAA 3 cut(s) 62, 540, 572
TspRI CASTG 3 cut(s) 39, 253, 526
XapI RAATTY 3 cut(s) 49, 331, 624
XceI RCATGY 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.