MD05G1324300.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011830
Physical Location & Seq
Forward (+)
45110314 .. 45112379
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324300.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTCTTGGTTAGCTTCTCTGAAGATTGTCCTAATATCGAGTGGCGTCGTGGCGTTGGCTCTGGCCATGAAGCTCTCTGTTCCGATGGCGACGGAGTTCGTAGCATCTCACTTGCCGGTCATGTGCTCCTACTTCCGATCCTTGTTCCGTCCTCCGTACCTCTACGTCCTTATCAACTGCATCATCATCACCATCGCCGCTTCCTCCAGGTTCAATGGCCACGGCAGGTCGCCTCAGCCGAAGCTTCAACCGGAGTCGGAGGCGAGCGTTTCGGATCCTCCGGCGGTTTACGAGTCAATGTATCGAGAGTCGGAGGTTGTTCATGAGCTGAAGTATGCGGTTGTGATCAAACCGGAGTCGGAGGCCATTTACGAACCGAGGTATCGAGAGCCTGAGGTTGTTCATGAGCTGACATCGGAGTCGGAGGTGGTTTCCGAACAGAGGTATCGAGAGCCTGAGGTTGTTCATGAGCTGACATCGGAGTCGGAGGTGGTTTCCGAACCGAGGTATCGAGAGCCTGAGGTTGTTTATGAGCTGACGTCTGCGGTTGTGATCAATGGATATGTAGAAGAAGCAGAGCCTGAGGCGGCTGAGATTAAAGATGATCGTAAATACGAAGACAAGCTTGTGATCTTGCCGCCGAAGAGTATGTTGGAGTCCGACGAGATTGCTTTGCCGGTGGAGAAACCTCCAGTTTCGGCGAGATTCAGTCACCGGAAACCGGCCAGGTCCATTCCTGAAGGTGGTAGGCCGTTGAGGGTGGCGAAGCCGAAACGGAACGACACGCTGGAGAACACGTGGAAGGCGATAACGGAAGGTCGGTCGATGCCGTTGAGCAGGCACACGAAGAAGACCGAGACGTTGCCGAATCACGGCCGTCAGTTAAAAGTTGACTTGACGTCGGCAGTGGACACGTCAGTGATGGTGAAGGCAGAGACGTTTAAGGAACCAACCAACCAAAGCCTGACAACTGTCGCCACCGGGGATGGAAGTGGAGGGAGGCTGAGGAAAGAGCCGTCGCTGAGTCAGGACGAGTTGAACCGCCAAGTGGAGGCGTTCATAAACAAGTTCAACGATGAAATGAGGATGCAGAGACAGCAGTCTTTGGATCAGTACAAGAAGATGGTCAACGGTGGAAGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

382

Amino Acids

42.95

Weight (kDa)

5.85

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 39 - 71 1.1e-14 Domain of unknown function (DUF4408)
DUF761 PF05553 343 - 377 1.3e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61260
fragaria_vesca FvH4_3g04160
malus_domestica MD05G1324300.v1.1 MD10G1302100.v1.1
prunus_persica Prupe.4G035700_v2.0.a1
pyrus_communis pycom05g29850 pycom10g25460
rosa_chinensis RchiOBHm_Chr5g0006611
rosa_laevigata RLG00000031406
rosa_multiflora Rmu_co8099104.1_g000001 Rmu_sc0001972.1_g000005 Rmu_sc0011154.1_g000002
rosa_roxburghii Rroxscaffold_1G00069400
rosa_rugosa Rorug04G0423100
rosa_samantha Rh5AG050500 Rh5BG049600 Rh5CG058100 Rh5DG049000
rosa_wichuraiana Rw5G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 418, 481
AatII GACGTC 2 cut(s) 542, 902
Acc36I ACCTGC 1 cut(s) 216
AciI CCGC 7 cut(s) 198, 284, 338, 545, 587, 638, 1042
AclWI GGATC 4 cut(s) 132, 269, 282, 1116
AcoI YGGCCR 4 cut(s) 63, 217, 723, 874
AcuI CTGAAG 3 cut(s) 41, 350, 759
AcvI CACGTG 1 cut(s) 798
AcyI GRCGYC 3 cut(s) 45, 539, 899
AfaI GTAC 2 cut(s) 158, 1115
AfiI CCNNNNNNNGG 7 cut(s) 441, 504, 721, 743, 872, 1048, 1051
AflIII ACRYGT 2 cut(s) 795, 912
AgsI TTSAA 4 cut(s) 214, 248, 1039, 1072
AhdI GACNNNNNGTC 1 cut(s) 898
AjiI CACGTC 1 cut(s) 915
AjnI CCWGG 2 cut(s) 206, 725
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AleI CACNNNNGTG 1 cut(s) 917
AluBI AGCT 8 cut(s) 14, 73, 244, 328, 409, 472, 535, 625
AluI AGCT 8 cut(s) 14, 73, 244, 328, 409, 472, 535, 625
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 3 cut(s) 851, 929, 1087
AlwI GGATC 4 cut(s) 132, 269, 282, 1116
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 6 cut(s) 63, 217, 363, 723, 749, 874
AspS9I GGNCC 1 cut(s) 729
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 3 cut(s) 181, 704, 937
AvaII GGWCC 1 cut(s) 729
AxyI CCTNAGG 4 cut(s) 393, 456, 519, 582
BaeI ACNNNNGTAYC 6 cut(s) 365, 398, 428, 461, 491, 524
BalI TGGCCA 2 cut(s) 65, 219
BamHI GGATCC 1 cut(s) 274
BbrPI CACGTG 1 cut(s) 798
BbsI GAAGAC 2 cut(s) 624, 857
Bbv12I GWGCWC 1 cut(s) 128
BbvCI CCTCAGC 2 cut(s) 234, 1004
BccI CCATC 5 cut(s) 79, 200, 916, 980, 1117
BceAI ACGGC 6 cut(s) 238, 736, 814, 861, 889, 1000
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BciT130I CCWGG 2 cut(s) 208, 727
BclI TGATCA 2 cut(s) 345, 552
BcnI CCSGG 1 cut(s) 982
BcoDI GTCTC 3 cut(s) 851, 929, 1087
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 3 cut(s) 198, 588, 638
BlsI GCNGC 3 cut(s) 199, 589, 639
Bme1390I CCNGG 3 cut(s) 208, 727, 982
Bme18I GGWCC 1 cut(s) 729
BmeRI GACNNNNNGTC 1 cut(s) 898
BmgBI CACGTC 1 cut(s) 915
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 2 cut(s) 276, 948
BmrFI CCNGG 3 cut(s) 208, 727, 982
BmsI GCATC 4 cut(s) 113, 189, 816, 1077
BoxI GACNNNNGTC 2 cut(s) 971, 1099
BpiI GAAGAC 2 cut(s) 624, 857
BpmI CTGGAG 3 cut(s) 190, 675, 809
Bpu10I CCTNAGC 2 cut(s) 234, 1004
BpuMI CCSGG 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 798
BsaHI GRCGYC 3 cut(s) 45, 539, 899
BsaJI CCNNGG 4 cut(s) 220, 377, 503, 981
BsaWI WCCGGW 3 cut(s) 250, 352, 714
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 7 cut(s) 441, 504, 721, 743, 872, 1048, 1051
Bse118I RCCGGY 3 cut(s) 115, 676, 721
Bse1I ACTGG 1 cut(s) 692
Bse21I CCTNAGG 4 cut(s) 393, 456, 519, 582
BseBI CCWGG 2 cut(s) 208, 727
BseDI CCNNGG 4 cut(s) 220, 377, 503, 981
BseGI GGATG 2 cut(s) 991, 1092
BseLI CCNNNNNNNGG 7 cut(s) 441, 504, 721, 743, 872, 1048, 1051
BseMII CTCAG 8 cut(s) 248, 384, 447, 510, 573, 582, 995, 1013
BseNI ACTGG 1 cut(s) 692
BseX3I CGGCCG 1 cut(s) 874
Bsh1285I CGRYCG 2 cut(s) 824, 877
BshFI GGCC 6 cut(s) 65, 219, 365, 725, 751, 876
BsiEI CGRYCG 2 cut(s) 824, 877
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 8 cut(s) 116, 251, 281, 353, 677, 715, 722, 981
BslI CCNNNNNNNGG 7 cut(s) 441, 504, 721, 743, 872, 1048, 1051
BsmAI GTCTC 3 cut(s) 851, 929, 1087
BsmBI CGTCTC 2 cut(s) 851, 929
BsnI GGCC 6 cut(s) 65, 219, 365, 725, 751, 876
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
BspACI CCGC 7 cut(s) 198, 284, 338, 545, 587, 638, 1042
BspANI GGCC 6 cut(s) 65, 219, 365, 725, 751, 876
BspCNI CTCAG 8 cut(s) 247, 385, 448, 511, 574, 583, 996, 1014
BspHI TCATGA 3 cut(s) 322, 403, 466
BspLI GGNNCC 2 cut(s) 276, 948
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 4 cut(s) 132, 269, 282, 1116
BsrFI RCCGGY 3 cut(s) 115, 676, 721
BsrI ACTGG 1 cut(s) 692
BssAI RCCGGY 3 cut(s) 115, 676, 721
BssECI CCNNGG 4 cut(s) 220, 377, 503, 981
BssMI GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
BssNI GRCGYC 3 cut(s) 45, 539, 899
Bst2UI CCWGG 2 cut(s) 208, 727
Bst4CI ACNGT 2 cut(s) 973, 1133
Bst6I CTCTTC 1 cut(s) 638
BstACI GRCGYC 3 cut(s) 45, 539, 899
BstBAI YACGTR 1 cut(s) 798
BstC8I GCNNGC 2 cut(s) 265, 839
BstDEI CTNAG 8 cut(s) 234, 393, 456, 519, 582, 591, 1004, 1022
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 2 cut(s) 991, 1092
BstKTI GATC 7 cut(s) 140, 277, 348, 555, 607, 633, 1111
BstMAI GTCTC 3 cut(s) 851, 929, 1087
BstMBI GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
BstMCI CGRYCG 2 cut(s) 824, 877
BstMWI GCNNNNNNNGC 1 cut(s) 1096
BstNI CCWGG 2 cut(s) 208, 727
BstPAI GACNNNNGTC 2 cut(s) 971, 1099
BstSCI CCNGG 3 cut(s) 206, 725, 980
BstV2I GAAGAC 2 cut(s) 624, 857
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BstZI CGGCCG 1 cut(s) 874
Bsu36I CCTNAGG 4 cut(s) 393, 456, 519, 582
BsuRI GGCC 6 cut(s) 65, 219, 365, 725, 751, 876
BtgI CCRYGG 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 178
BtrI CACGTC 1 cut(s) 915
BtsCI GGATG 2 cut(s) 991, 1092
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 2 cut(s) 912, 924
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 265, 839
CaiI CAGNNNCTG 1 cut(s) 581
CciI TCATGA 3 cut(s) 322, 403, 466
Cfr10I RCCGGY 3 cut(s) 115, 676, 721
Cfr13I GGNCC 1 cut(s) 729
CseI GACGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 157, 1114
CviAII CATG 5 cut(s) 67, 121, 323, 404, 467
CviQI GTAC 2 cut(s) 157, 1114
DdeI CTNAG 8 cut(s) 234, 393, 456, 519, 582, 591, 1004, 1022
DpnI GATC 7 cut(s) 139, 276, 347, 554, 606, 632, 1110
DpnII GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
DrdI GACNNNNNNGTC 2 cut(s) 418, 481
DriI GACNNNNNGTC 1 cut(s) 898
DseDI GACNNNNNNGTC 2 cut(s) 418, 481
EaeI YGGCCR 4 cut(s) 63, 217, 723, 874
EagI CGGCCG 1 cut(s) 874
Eam1104I CTCTTC 1 cut(s) 638
Eam1105I GACNNNNNGTC 1 cut(s) 898
EarI CTCTTC 1 cut(s) 638
EclXI CGGCCG 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 729
Eco52I CGGCCG 1 cut(s) 874
Eco57I CTGAAG 3 cut(s) 41, 350, 759
Eco72I CACGTG 1 cut(s) 798
Eco81I CCTNAGG 4 cut(s) 393, 456, 519, 582
EcoRII CCWGG 2 cut(s) 206, 725
Esp3I CGTCTC 2 cut(s) 851, 929
FaeI CATG 5 cut(s) 70, 124, 326, 407, 470
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 5 cut(s) 66, 120, 322, 403, 466
FbaI TGATCA 2 cut(s) 345, 552
Fnu4HI GCNGC 3 cut(s) 198, 588, 638
FokI GGATG 2 cut(s) 998, 1099
Fsp4HI GCNGC 3 cut(s) 198, 588, 638
GluI GCNGC 3 cut(s) 198, 588, 638
GsuI CTGGAG 3 cut(s) 190, 675, 809
HaeIII GGCC 6 cut(s) 65, 219, 365, 725, 751, 876
HapII CCGG 8 cut(s) 116, 251, 281, 353, 677, 715, 722, 981
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 3 cut(s) 45, 539, 899
Hin1II CATG 5 cut(s) 70, 124, 326, 407, 470
HincII GTYRAC 2 cut(s) 892, 1129
HindII GTYRAC 2 cut(s) 892, 1129
HindIII AAGCTT 2 cut(s) 242, 623
HpaII CCGG 8 cut(s) 116, 251, 281, 353, 677, 715, 722, 981
HphI GGTGA 3 cut(s) 181, 704, 937
Hpy166II GTNNAC 4 cut(s) 289, 892, 910, 1129
Hpy188III TCNNGA 9 cut(s) 305, 323, 386, 404, 449, 467, 512, 737, 1028
Hpy8I GTNNAC 4 cut(s) 289, 892, 910, 1129
Hpy99I CGWCG 5 cut(s) 50, 94, 665, 904, 1021
HpyAV CCTTC 4 cut(s) 734, 796, 809, 922
HpyCH4III ACNGT 2 cut(s) 973, 1133
HpyCH4IV ACGT 7 cut(s) 165, 539, 797, 860, 899, 914, 938
HpyCH4V TGCA 2 cut(s) 180, 1090
HpyF10VI GCNNNNNNNGC 1 cut(s) 1096
HpyF3I CTNAG 8 cut(s) 234, 393, 456, 519, 582, 591, 1004, 1022
HpySE526I ACGT 7 cut(s) 165, 539, 797, 860, 899, 914, 938
Hsp92I GRCGYC 3 cut(s) 45, 539, 899
Hsp92II CATG 5 cut(s) 70, 124, 326, 407, 470
Ksp22I TGATCA 2 cut(s) 345, 552
Kzo9I GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
LmnI GCTCC 1 cut(s) 131
LweI GCATC 4 cut(s) 113, 189, 816, 1077
MaeII ACGT 7 cut(s) 165, 539, 797, 860, 899, 914, 938
MaeIII GTNAC 1 cut(s) 710
MalI GATC 7 cut(s) 139, 276, 347, 554, 606, 632, 1110
MboI GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
MboII GAAGA 7 cut(s) 34, 581, 629, 655, 859, 862, 1132
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 128
MlsI TGGCCA 2 cut(s) 65, 219
MluNI TGGCCA 2 cut(s) 65, 219
MlyI GAGTC 8 cut(s) 263, 302, 317, 365, 428, 491, 665, 1033
MmeI TCCRAC 7 cut(s) 237, 291, 339, 402, 465, 633, 684
Mox20I TGGCCA 2 cut(s) 65, 219
MscI TGGCCA 2 cut(s) 65, 219
MseI TTAA 3 cut(s) 597, 884, 942
MslI CAYNNNNRTG 1 cut(s) 917
Msp20I TGGCCA 2 cut(s) 65, 219
MspI CCGG 8 cut(s) 116, 251, 281, 353, 677, 715, 722, 981
MspR9I CCNGG 3 cut(s) 208, 727, 982
MvaI CCWGG 2 cut(s) 208, 727
MwoI GCNNNNNNNGC 1 cut(s) 1096
NciI CCSGG 1 cut(s) 982
NdeII GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
NlaIII CATG 5 cut(s) 70, 124, 326, 407, 470
NlaIV GGNNCC 2 cut(s) 276, 948
NmuCI GTSAC 1 cut(s) 710
OliI CACNNNNGTG 1 cut(s) 917
PagI TCATGA 3 cut(s) 322, 403, 466
PcsI WCGNNNNNNNCGW 2 cut(s) 236, 803
PfeI GAWTC 2 cut(s) 705, 868
PkrI GCNGC 3 cut(s) 199, 589, 639
PleI GAGTC 8 cut(s) 262, 301, 316, 364, 427, 490, 664, 1032
PmaCI CACGTG 1 cut(s) 798
PmlI CACGTG 1 cut(s) 798
PpsI GAGTC 8 cut(s) 262, 301, 316, 364, 427, 490, 664, 1032
Ppu21I YACGTR 1 cut(s) 798
PshAI GACNNNNGTC 2 cut(s) 971, 1099
Psp6I CCWGG 2 cut(s) 206, 725
PspCI CACGTG 1 cut(s) 798
PspGI CCWGG 2 cut(s) 206, 725
PspN4I GGNNCC 2 cut(s) 276, 948
PspPI GGNCC 1 cut(s) 729
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 2 cut(s) 158, 1115
RsaNI GTAC 2 cut(s) 157, 1114
RseI CAYNNNNRTG 1 cut(s) 917
SaqAI TTAA 3 cut(s) 597, 884, 942
SatI GCNGC 3 cut(s) 198, 588, 638
Sau3AI GATC 7 cut(s) 137, 274, 345, 552, 604, 630, 1108
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 8 cut(s) 263, 302, 317, 365, 428, 491, 665, 1033
ScrFI CCNGG 3 cut(s) 208, 727, 982
SduI GDGCHC 1 cut(s) 128
SfaNI GCATC 4 cut(s) 113, 189, 816, 1077
SinI GGWCC 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 917
SsiI CCGC 7 cut(s) 198, 284, 338, 545, 587, 638, 1042
StyD4I CCNGG 3 cut(s) 206, 725, 980
TaaI ACNGT 2 cut(s) 973, 1133
TaiI ACGT 7 cut(s) 168, 542, 800, 863, 902, 917, 941
TaqI TCGA 6 cut(s) 38, 304, 385, 448, 511, 824
TaqII GACCGA 2 cut(s) 810, 869
TatI WGTACW 1 cut(s) 1113
TauI GCSGC 3 cut(s) 200, 590, 640
TfiI GAWTC 2 cut(s) 705, 868
Tru1I TTAA 3 cut(s) 597, 884, 942
Tru9I TTAA 3 cut(s) 597, 884, 942
TscAI CASTG 2 cut(s) 912, 924
TseFI GTSAC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 6 cut(s) 83, 311, 392, 455, 1048, 1092
TspGWI ACGGA 5 cut(s) 107, 137, 144, 790, 827
TspRI CASTG 2 cut(s) 912, 924
VpaK11BI GGWCC 1 cut(s) 729
ZraI GACGTC 2 cut(s) 540, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.