MD10G1302100.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011830
Physical Location & Seq
Forward (+)
38903045 .. 38904936
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1302100.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGTCTTGGTTAGCTTCTCTGAAGATTGTCCTGATTTTGAGCGGCTTTGTGGCGTGGGCTCTGGCCATGAAGATCTCCGTTCCGATGGCGGCGGAGTTCGTAGCCTCTAACTTTTCGGTCATGTGCTCCTCATTCCGATTCTTGCTCCGCCCTCCGTACCTCTACGTCCTCGTCAACGGCATCATCATCACCATCGCCGCTTCCTCCAGATTCGACGGCCACGGCAGGTCGCCTCAGCCGAAGCTTCAACCGGAGTCGGAGGTGAGCGTTTCGGATCCGGCGGTGGTTTACGCGCCGATGTATCGAGAGCCGAAGGTTCATAAGCTGACGTCTGCGGTTGTGATCAAACCGGGGTCGGAGGCGGTTTACCAACCGAGGTATCGAGAGCCGGAGGTTGTTCATGAGCCGACGTCTGCGTTTGTGAGCAATGGCTACGTAGAAGAAGCAAAGCCTGCGGTGGCTGAGAATAAAGATGATCGCGAATACGAAGATAAGCTTGTGATCTCGCCGCCGAAGAGTATGGTGGAGTCCGATGATATCGCTTTGCCGGTGGAGAAACCTCTAGTTTCGGCGAGACACAGTCGCCGGAAACCGGCTAGATCCAGTCGCCGTAAACCGGCTAGATCCAGTCCTGAAGGTGGGGAGCCTTTGATAGTGGTGAAGCCAAAGCAGTACGACACGCTGGAGAGCACGTGGAAGGCGATAACGGAAGGTCGGTCGAATACGTTGAGCAGGCGCATGGAGAAGTCCGAGACGTTGCAGAACTGTGGCCGTCAGTTAAACGTTGACTTGACGTCGGCAGGGGACACGCCAGTGATGGCGAAGGCAGAGACGTTTAAGGACCGAAGCAACCAGAAACTGAGACGTGCAGCCGCCGTGGATGGAAGTGGAGGGAGGCTGAGGAAAGAGCCGTCGCTGAGTCAGGACGAGTTGAACCGCCGAGTGGAAGAGTTCATAAACAAGTTCAATGAGGAAATGAGGCTGCAGAGACAAGAGTCTTTGAATCAGTACATGAAAATGGTCAACCGTGGAAGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

347

Amino Acids

38.81

Weight (kDa)

9.46

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 41 - 71 2.1e-14 Domain of unknown function (DUF4408)
DUF761 PF05553 308 - 343 2.4e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61260
fragaria_vesca FvH4_3g04160
malus_domestica MD05G1324300.v1.1 MD10G1302100.v1.1
prunus_persica Prupe.4G035700_v2.0.a1
pyrus_communis pycom05g29850 pycom10g25460
rosa_chinensis RchiOBHm_Chr5g0006611
rosa_laevigata RLG00000031406
rosa_multiflora Rmu_co8099104.1_g000001 Rmu_sc0001972.1_g000005 Rmu_sc0011154.1_g000002
rosa_roxburghii Rroxscaffold_1G00069400
rosa_rugosa Rorug04G0423100
rosa_samantha Rh5AG050500 Rh5BG049600 Rh5CG058100 Rh5DG049000
rosa_wichuraiana Rw5G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 332, 413, 797
Acc36I ACCTGC 1 cut(s) 216
AccBSI CCGCTC 1 cut(s) 42
AccII CGCG 2 cut(s) 293, 480
AclI AACGTT 1 cut(s) 783
AclWI GGATC 4 cut(s) 269, 282, 594, 618
AcoI YGGCCR 3 cut(s) 63, 217, 769
AcuI CTGAAG 2 cut(s) 41, 654
AcvI CACGTG 1 cut(s) 693
AcyI GRCGYC 3 cut(s) 329, 410, 794
AfaI GTAC 3 cut(s) 158, 674, 1010
AfiI CCNNNNNNNGG 4 cut(s) 592, 616, 638, 943
AgsI TTSAA 4 cut(s) 248, 934, 967, 1003
AhdI GACNNNNNGTC 1 cut(s) 793
AjiI CACGTC 1 cut(s) 866
AleI CACNNNNGTG 1 cut(s) 812
AluBI AGCT 4 cut(s) 14, 244, 325, 496
AluI AGCT 4 cut(s) 14, 244, 325, 496
Alw21I GWGCWC 2 cut(s) 128, 692
Alw26I GTCTC 5 cut(s) 568, 746, 824, 856, 982
AlwI GGATC 4 cut(s) 269, 282, 594, 618
AlwNI CAGNNNCTG 1 cut(s) 859
AoxI GGCC 3 cut(s) 63, 217, 769
ApeKI GCWGC 2 cut(s) 869, 982
AspLEI GCGC 2 cut(s) 295, 738
AspS9I GGNCC 1 cut(s) 841
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 3 cut(s) 181, 274, 670
AvaII GGWCC 1 cut(s) 841
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 61
BbrPI CACGTG 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 128, 692
BbvCI CCTCAGC 2 cut(s) 234, 899
BbvI GCAGC 2 cut(s) 881, 969
BccI CCATC 4 cut(s) 79, 200, 811, 875
BceAI ACGGC 7 cut(s) 193, 232, 238, 594, 756, 860, 895
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 5 cut(s) 568, 746, 824, 856, 982
BfaI CTAG 3 cut(s) 563, 597, 621
BfmI CTRYAG 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 216
BglII AGATCT 1 cut(s) 72
BisI GCNGC 7 cut(s) 43, 90, 198, 509, 870, 873, 983
BlsI GCNGC 7 cut(s) 44, 91, 199, 510, 871, 874, 984
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 841
BmeRI GACNNNNNGTC 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 866
BmgT120I GGNCC 1 cut(s) 841
BmiI GGNNCC 2 cut(s) 276, 645
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 189
BoxI GACNNNNGTC 1 cut(s) 994
BpmI CTGGAG 2 cut(s) 190, 704
Bpu10I CCTNAGC 2 cut(s) 234, 899
BpuMI CCSGG 1 cut(s) 351
BsaAI YACGTR 2 cut(s) 436, 693
BsaHI GRCGYC 3 cut(s) 329, 410, 794
BsaJI CCNNGG 5 cut(s) 220, 350, 374, 876, 1027
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 4 cut(s) 592, 616, 638, 943
Bse118I RCCGGY 3 cut(s) 547, 592, 616
Bse1I ACTGG 3 cut(s) 603, 627, 812
Bse3DI GCAATG 1 cut(s) 433
BseDI CCNNGG 5 cut(s) 220, 350, 374, 876, 1027
BseGI GGATG 1 cut(s) 886
BseLI CCNNNNNNNGG 4 cut(s) 592, 616, 638, 943
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 5 cut(s) 248, 453, 851, 890, 908
BseNI ACTGG 3 cut(s) 603, 627, 812
BseRI GAGGAG 1 cut(s) 118
BseXI GCAGC 2 cut(s) 881, 969
BsgI GTGCAG 1 cut(s) 888
Bsh1236I CGCG 2 cut(s) 293, 480
Bsh1285I CGRYCG 1 cut(s) 719
BshFI GGCC 3 cut(s) 65, 219, 771
BsiEI CGRYCG 1 cut(s) 719
BsiHKAI GWGCWC 2 cut(s) 128, 692
BsiSI CCGG 8 cut(s) 251, 278, 350, 389, 548, 586, 593, 617
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 4 cut(s) 592, 616, 638, 943
BsmAI GTCTC 5 cut(s) 568, 746, 824, 856, 982
BsmBI CGTCTC 3 cut(s) 746, 824, 856
BsmFI GGGAC 1 cut(s) 818
BsnI GGCC 3 cut(s) 65, 219, 771
Bsp1286I GDGCHC 3 cut(s) 61, 128, 692
Bsp143I GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
Bsp68I TCGCGA 1 cut(s) 480
BspANI GGCC 3 cut(s) 65, 219, 771
BspCNI CTCAG 5 cut(s) 247, 454, 852, 891, 909
BspFNI CGCG 2 cut(s) 293, 480
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 276, 645
BspMAI CTGCAG 1 cut(s) 987
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 4 cut(s) 269, 282, 594, 618
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 3 cut(s) 547, 592, 616
BsrI ACTGG 3 cut(s) 603, 627, 812
BssAI RCCGGY 3 cut(s) 547, 592, 616
BssECI CCNNGG 5 cut(s) 220, 350, 374, 876, 1027
BssMI GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
BssNI GRCGYC 3 cut(s) 329, 410, 794
Bst4CI ACNGT 3 cut(s) 581, 767, 1028
Bst6I CTCTTC 2 cut(s) 509, 942
BstACI GRCGYC 3 cut(s) 329, 410, 794
BstAPI GCANNNNNTGC 1 cut(s) 452
BstBAI YACGTR 2 cut(s) 436, 693
BstC8I GCNNGC 2 cut(s) 453, 734
BstDEI CTNAG 5 cut(s) 234, 462, 860, 899, 917
BstDSI CCRYGG 3 cut(s) 220, 876, 1027
BstF5I GGATG 1 cut(s) 886
BstFNI CGCG 2 cut(s) 293, 480
BstHHI GCGC 2 cut(s) 295, 738
BstKTI GATC 7 cut(s) 75, 277, 345, 478, 504, 602, 626
BstMAI GTCTC 5 cut(s) 568, 746, 824, 856, 982
BstMBI GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
BstMCI CGRYCG 1 cut(s) 719
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstPAI GACNNNNGTC 1 cut(s) 994
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 983
BstSNI TACGTA 1 cut(s) 436
BstUI CGCG 2 cut(s) 293, 480
BstV1I GCAGC 2 cut(s) 881, 969
BstX2I RGATCY 4 cut(s) 72, 274, 599, 623
BstYI RGATCY 4 cut(s) 72, 274, 599, 623
BsuRI GGCC 3 cut(s) 65, 219, 771
BtgI CCRYGG 3 cut(s) 220, 876, 1027
BtgZI GCGATG 1 cut(s) 178
BtrI CACGTC 1 cut(s) 866
BtsCI GGATG 1 cut(s) 886
BtsIMutI CAGTG 1 cut(s) 819
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 453, 734
CaiI CAGNNNCTG 1 cut(s) 859
CciI TCATGA 1 cut(s) 400
CfoI GCGC 2 cut(s) 295, 738
Cfr10I RCCGGY 3 cut(s) 547, 592, 616
Cfr13I GGNCC 1 cut(s) 841
Csp6I GTAC 3 cut(s) 157, 673, 1009
CviAII CATG 5 cut(s) 67, 121, 401, 739, 1012
CviQI GTAC 3 cut(s) 157, 673, 1009
DdeI CTNAG 5 cut(s) 234, 462, 860, 899, 917
DpnI GATC 7 cut(s) 74, 276, 344, 477, 503, 601, 625
DpnII GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
DriI GACNNNNNGTC 1 cut(s) 793
EaeI YGGCCR 3 cut(s) 63, 217, 769
Eam1104I CTCTTC 2 cut(s) 509, 942
Eam1105I GACNNNNNGTC 1 cut(s) 793
EarI CTCTTC 2 cut(s) 509, 942
EciI GGCGGA 2 cut(s) 107, 137
Eco105I TACGTA 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 841
Eco57I CTGAAG 2 cut(s) 41, 654
Eco72I CACGTG 1 cut(s) 693
EcoRV GATATC 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 61
Esp3I CGTCTC 3 cut(s) 746, 824, 856
FaeI CATG 5 cut(s) 70, 124, 404, 742, 1015
FaiI YATR 8 cut(s) 68, 122, 321, 402, 521, 740, 956, 1013
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 1 cut(s) 818
FatI CATG 5 cut(s) 66, 120, 400, 738, 1011
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 7 cut(s) 43, 90, 198, 509, 870, 873, 983
FokI GGATG 1 cut(s) 893
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 7 cut(s) 43, 90, 198, 509, 870, 873, 983
FspBI CTAG 3 cut(s) 563, 597, 621
GlaI GCGC 2 cut(s) 294, 737
GluI GCNGC 7 cut(s) 43, 90, 198, 509, 870, 873, 983
GsuI CTGGAG 2 cut(s) 190, 704
HaeIII GGCC 3 cut(s) 65, 219, 771
HapII CCGG 8 cut(s) 251, 278, 350, 389, 548, 586, 593, 617
HhaI GCGC 2 cut(s) 295, 738
Hin1I GRCGYC 3 cut(s) 329, 410, 794
Hin1II CATG 5 cut(s) 70, 124, 404, 742, 1015
Hin6I GCGC 2 cut(s) 293, 736
HinP1I GCGC 2 cut(s) 293, 736
HincII GTYRAC 3 cut(s) 175, 787, 1024
HindII GTYRAC 3 cut(s) 175, 787, 1024
HindIII AAGCTT 2 cut(s) 242, 494
HinfI GANTC 7 cut(s) 138, 210, 254, 527, 919, 995, 1003
HpaII CCGG 8 cut(s) 251, 278, 350, 389, 548, 586, 593, 617
HphI GGTGA 3 cut(s) 181, 274, 670
Hpy166II GTNNAC 6 cut(s) 175, 289, 367, 614, 787, 1024
Hpy188I TCNGA 8 cut(s) 21, 84, 137, 259, 274, 358, 532, 751
Hpy188III TCNNGA 8 cut(s) 31, 207, 305, 383, 401, 479, 632, 923
Hpy8I GTNNAC 6 cut(s) 175, 289, 367, 614, 787, 1024
Hpy99I CGWCG 4 cut(s) 218, 412, 799, 916
HpyAV CCTTC 5 cut(s) 307, 629, 691, 704, 817
HpyCH4III ACNGT 3 cut(s) 581, 767, 1028
HpyCH4V TGCA 3 cut(s) 760, 869, 985
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 5 cut(s) 234, 462, 860, 899, 917
Hsp92I GRCGYC 3 cut(s) 329, 410, 794
Hsp92II CATG 5 cut(s) 70, 124, 404, 742, 1015
HspAI GCGC 2 cut(s) 293, 736
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
LmnI GCTCC 3 cut(s) 131, 150, 643
Lsp1109I GCAGC 2 cut(s) 881, 969
LweI GCATC 1 cut(s) 189
MaeI CTAG 3 cut(s) 563, 597, 621
MalI GATC 7 cut(s) 74, 276, 344, 477, 503, 601, 625
MbiI CCGCTC 1 cut(s) 42
MboI GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
MboII GAAGA 6 cut(s) 34, 82, 452, 500, 526, 959
MflI RGATCY 4 cut(s) 72, 274, 599, 623
MhlI GDGCHC 3 cut(s) 61, 128, 692
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 4 cut(s) 263, 536, 928, 1004
MmeI TCCRAC 2 cut(s) 237, 336
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 2 cut(s) 779, 837
MslI CAYNNNNRTG 2 cut(s) 812, 1016
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 8 cut(s) 251, 278, 350, 389, 548, 586, 593, 617
MspR9I CCNGG 1 cut(s) 351
MvnI CGCG 2 cut(s) 293, 480
MwoI GCNNNNNNNGC 1 cut(s) 452
NciI CCSGG 1 cut(s) 351
NdeII GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
NlaIII CATG 5 cut(s) 70, 124, 404, 742, 1015
NlaIV GGNNCC 2 cut(s) 276, 645
NmeAIII GCCGAG 1 cut(s) 965
NruI TCGCGA 1 cut(s) 480
OliI CACNNNNGTG 1 cut(s) 812
PagI TCATGA 1 cut(s) 400
PcsI WCGNNNNNNNCGW 2 cut(s) 236, 698
PfeI GAWTC 3 cut(s) 138, 210, 1003
PflFI GACNNNGTC 1 cut(s) 579
PkrI GCNGC 7 cut(s) 44, 91, 199, 510, 871, 874, 984
PleI GAGTC 4 cut(s) 262, 535, 927, 1003
PmaCI CACGTG 1 cut(s) 693
PmlI CACGTG 1 cut(s) 693
PpsI GAGTC 4 cut(s) 262, 535, 927, 1003
Ppu21I YACGTR 2 cut(s) 436, 693
PshAI GACNNNNGTC 1 cut(s) 994
Psp1406I AACGTT 1 cut(s) 783
PspCI CACGTG 1 cut(s) 693
PspN4I GGNNCC 2 cut(s) 276, 645
PspPI GGNCC 1 cut(s) 841
PstI CTGCAG 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 859
PsuI RGATCY 4 cut(s) 72, 274, 599, 623
PsyI GACNNNGTC 1 cut(s) 579
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 3 cut(s) 158, 674, 1010
RsaNI GTAC 3 cut(s) 157, 673, 1009
RseI CAYNNNNRTG 2 cut(s) 812, 1016
SaqAI TTAA 2 cut(s) 779, 837
SatI GCNGC 7 cut(s) 43, 90, 198, 509, 870, 873, 983
Sau3AI GATC 7 cut(s) 72, 274, 342, 475, 501, 599, 623
Sau96I GGNCC 1 cut(s) 841
SchI GAGTC 4 cut(s) 263, 536, 928, 1004
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 3 cut(s) 61, 128, 692
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 983
SinI GGWCC 1 cut(s) 841
SmiMI CAYNNNNRTG 2 cut(s) 812, 1016
SnaBI TACGTA 1 cut(s) 436
SspMI CTAG 3 cut(s) 563, 597, 621
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 3 cut(s) 581, 767, 1028
TaqI TCGA 4 cut(s) 213, 304, 382, 719
TaqII GACCGA 3 cut(s) 106, 705, 858
TatI WGTACW 1 cut(s) 1008
TauI GCSGC 5 cut(s) 45, 92, 200, 511, 875
TfiI GAWTC 3 cut(s) 138, 210, 1003
Tru1I TTAA 2 cut(s) 779, 837
Tru9I TTAA 2 cut(s) 779, 837
TscAI CASTG 1 cut(s) 819
TseI GCWGC 2 cut(s) 869, 982
TspDTI ATGAA 5 cut(s) 83, 308, 389, 943, 1028
TspGWI ACGGA 3 cut(s) 67, 144, 722
TspRI CASTG 1 cut(s) 819
Tth111I GACNNNGTC 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 841
XspI CTAG 3 cut(s) 563, 597, 621
ZraI GACGTC 3 cut(s) 330, 411, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.