pycom05g29850

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011830
Physical Location & Seq
Forward (+)
30200952 .. 30203022
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29850.1

Sequence Viewer

Length: 1227 bp
ATGCAATTACGTTTCTCTCAAACGACCACCGCTCAGTGCTCACATCTCCAATTCCCAGTTTCCCACTCACTCTCTGTCTTAAACACTCACCTCCGCAATCACCGTAATCAATTAAAATCAAACTTGATTAAGATCTTAATTAACCAAAAAATGTCTTGGTTAGCTTCTCTGAAGATTGTTCTAATCTCGAGTGGCGTCGTGGCGTTGGCTCTGGCCATGAAGCTCTTTGTTCCGATGGCGACGGAGTTCGTAGCATCTCACGTGCCGGTCATGTGCTCCTCCTTCCTATCCTTGTTCCGTCCTCCGTACCTCTACGTCCTCATCAACTGCATCATCATCACCATCGCCGCTTCCTCCAGGTTCCAAGGCAGGTCGCATCAGCCGAAGCTTCAACCGGAGTCGGAGGCGAGCGTTTCAGAACCTCCGGCGGTTTACGAGCCGGTGTATAGAGAGCCGGAGGTTGTTCATGAGCTGAAGTCTGCGGTTGTGATCAAACCGGAGTCGGAGGCGGTTTACGAACCGAGGTATCGAGAGCCTGAGGTTGTCCATGAGCTGAAACCGGAGTCGGAGGTGGTTTCCGAACCGAGGTATCGAGAGCCTGAGGTTGTTTATGAGCTGACATCTGCGGTTGTGTTCAATGAGTATGTAGAAGAAGCAGAGCCTGAGGCGGCTGAGATTGACGATGGTCGTGAATACGAAGACAAGCTTGTGATCTTGCCGCCGAAGAGTATGGTGGAGTCCGATGCGATCACTTTGCCGGTGGAGAAACCTCCAGTTTCGGCGAGATTCAGTCACCGGAAACCGGCTAGGTCTATTCCTGAAGGTGGGAAGTCGTTGAGGGTGGCGAAGCCGAAACGGAACGACACGCTGGAGAACACGTGGAAAGCGATAACGGAAGGTCGGTCGATGCCGTTGAGCAGGCACACGAAGAAGTCCGAGACGTTGCCAAATCACGGCCGTCAGTTAAAAGTTGACTTGACGTCGGCAGTGGACACGTCAGTGATGGTGAAGGCAGAGACGGTTAAGGAACCAACCAACCAAAGCCTGACAGCTGTCGCCGCCGGGGATGGAAGTGGAGGGAGGCTGAGGAAAGAGCCGTCGCTGAGTCAGGACGACTTGAACCGCCAAGTGGAAGCGTTCATAAACAAGTTCAATGAGGAAATGAGGATGCAGAGACAACAGTCTTTGGATCACTACAAGAAGATGGTCAACGGTGGAAGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

409

Amino Acids

45.85

Weight (kDa)

7.24

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 89 - 121 2.1e-14 Domain of unknown function (DUF4408)
DUF761 PF05553 370 - 404 6.1e-14 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61260
fragaria_vesca FvH4_3g04160
malus_domestica MD05G1324300.v1.1 MD10G1302100.v1.1
prunus_persica Prupe.4G035700_v2.0.a1
pyrus_communis pycom05g29850 pycom10g25460
rosa_chinensis RchiOBHm_Chr5g0006611
rosa_laevigata RLG00000031406
rosa_multiflora Rmu_co8099104.1_g000001 Rmu_sc0001972.1_g000005 Rmu_sc0011154.1_g000002
rosa_roxburghii Rroxscaffold_1G00069400
rosa_rugosa Rorug04G0423100
rosa_samantha Rh5AG050500 Rh5BG049600 Rh5CG058100 Rh5DG049000
rosa_wichuraiana Rw5G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 983
Acc36I ACCTGC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 32
AclWI GGATC 1 cut(s) 1197
AcoI YGGCCR 2 cut(s) 213, 955
AcuI CTGAAG 3 cut(s) 191, 494, 840
AcvI CACGTG 2 cut(s) 262, 879
AcyI GRCGYC 2 cut(s) 195, 980
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 5 cut(s) 585, 802, 824, 953, 1129
AflIII ACRYGT 2 cut(s) 876, 993
AgsI TTSAA 4 cut(s) 392, 637, 1120, 1153
AhdI GACNNNNNGTC 1 cut(s) 979
AjiI CACGTC 1 cut(s) 996
AjnI CCWGG 1 cut(s) 356
AleI CACNNNNGTG 1 cut(s) 998
AluBI AGCT 8 cut(s) 164, 223, 388, 472, 553, 616, 706, 1052
AluI AGCT 8 cut(s) 164, 223, 388, 472, 553, 616, 706, 1052
Alw21I GWGCWC 2 cut(s) 41, 278
Alw26I GTCTC 3 cut(s) 932, 1010, 1168
AlwI GGATC 1 cut(s) 1197
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 2 cut(s) 213, 955
AsuC2I CCSGG 1 cut(s) 1063
AsuHPI GGTGA 5 cut(s) 80, 92, 331, 785, 1018
AvaI CYCGRG 1 cut(s) 187
AxyI CCTNAGG 3 cut(s) 537, 600, 663
BaeI ACNNNNGTAYC 4 cut(s) 509, 542, 572, 605
BalI TGGCCA 1 cut(s) 215
BbrPI CACGTG 2 cut(s) 262, 879
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 41, 278
BbvCI CCTCAGC 1 cut(s) 1085
BccI CCATC 6 cut(s) 229, 350, 677, 997, 1061, 1198
BceAI ACGGC 4 cut(s) 895, 942, 970, 1081
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 1063
BcoDI GTCTC 3 cut(s) 932, 1010, 1168
BfaI CTAG 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 360
BglII AGATCT 1 cut(s) 132
BisI GCNGC 4 cut(s) 348, 669, 719, 1059
BlsI GCNGC 4 cut(s) 349, 670, 720, 1060
Bme1390I CCNGG 2 cut(s) 358, 1063
BmeRI GACNNNNNGTC 1 cut(s) 979
BmeT110I CYCGRG 1 cut(s) 187
BmgBI CACGTC 1 cut(s) 996
BmiI GGNNCC 2 cut(s) 362, 1029
BmrFI CCNGG 2 cut(s) 358, 1063
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 6 cut(s) 263, 339, 385, 733, 897, 1158
BmuI ACTGGG 1 cut(s) 50
BoxI GACNNNNGTC 3 cut(s) 684, 1052, 1180
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 3 cut(s) 340, 756, 890
Bpu10I CCTNAGC 1 cut(s) 1085
BpuMI CCSGG 1 cut(s) 1063
BsaAI YACGTR 2 cut(s) 262, 879
BsaBI GATNNNNATC 1 cut(s) 131
BsaHI GRCGYC 2 cut(s) 195, 980
BsaJI CCNNGG 4 cut(s) 364, 521, 584, 1062
BsaWI WCCGGW 4 cut(s) 394, 496, 559, 795
BsaXI ACNNNNNCTCC 2 cut(s) 863, 893
Bsc4I CCNNNNNNNGG 5 cut(s) 585, 802, 824, 953, 1129
Bse118I RCCGGY 4 cut(s) 265, 439, 757, 802
Bse1I ACTGG 2 cut(s) 56, 773
Bse21I CCTNAGG 3 cut(s) 537, 600, 663
Bse8I GATNNNNATC 1 cut(s) 131
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 4 cut(s) 364, 521, 584, 1062
BseGI GGATG 2 cut(s) 1072, 1173
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 5 cut(s) 585, 802, 824, 953, 1129
BseMII CTCAG 7 cut(s) 47, 528, 591, 654, 663, 1076, 1094
BseNI ACTGG 2 cut(s) 56, 773
BseRI GAGGAG 1 cut(s) 268
BseX3I CGGCCG 1 cut(s) 955
Bsh1285I CGRYCG 2 cut(s) 905, 958
BshFI GGCC 2 cut(s) 215, 957
BsiEI CGRYCG 2 cut(s) 905, 958
BsiHKAI GWGCWC 2 cut(s) 41, 278
BsiHKCI CYCGRG 1 cut(s) 187
BslI CCNNNNNNNGG 5 cut(s) 585, 802, 824, 953, 1129
BsmAI GTCTC 3 cut(s) 932, 1010, 1168
BsmBI CGTCTC 2 cut(s) 932, 1010
BsnI GGCC 2 cut(s) 215, 957
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 41, 278
Bsp143I GATC 5 cut(s) 132, 489, 711, 747, 1189
BspANI GGCC 2 cut(s) 215, 957
BspCNI CTCAG 7 cut(s) 46, 529, 592, 655, 664, 1077, 1095
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 362, 1029
BspMI ACCTGC 1 cut(s) 360
BspPI GGATC 1 cut(s) 1197
BsrBI CCGCTC 1 cut(s) 32
BsrFI RCCGGY 4 cut(s) 265, 439, 757, 802
BsrI ACTGG 2 cut(s) 56, 773
BssAI RCCGGY 4 cut(s) 265, 439, 757, 802
BssECI CCNNGG 4 cut(s) 364, 521, 584, 1062
BssMI GATC 5 cut(s) 132, 489, 711, 747, 1189
BssNI GRCGYC 2 cut(s) 195, 980
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 4 cut(s) 104, 1021, 1182, 1214
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 2 cut(s) 195, 980
BstBAI YACGTR 2 cut(s) 262, 879
BstC8I GCNNGC 2 cut(s) 409, 920
BstDEI CTNAG 7 cut(s) 33, 537, 600, 663, 672, 1085, 1103
BstF5I GGATG 2 cut(s) 1072, 1173
BstKTI GATC 5 cut(s) 135, 492, 714, 750, 1192
BstMAI GTCTC 3 cut(s) 932, 1010, 1168
BstMBI GATC 5 cut(s) 132, 489, 711, 747, 1189
BstMCI CGRYCG 2 cut(s) 905, 958
BstMWI GCNNNNNNNGC 1 cut(s) 1058
BstNI CCWGG 1 cut(s) 358
BstPAI GACNNNNGTC 3 cut(s) 684, 1052, 1180
BstSCI CCNGG 2 cut(s) 356, 1061
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BstZI CGGCCG 1 cut(s) 955
Bsu36I CCTNAGG 3 cut(s) 537, 600, 663
BsuRI GGCC 2 cut(s) 215, 957
BtgZI GCGATG 1 cut(s) 328
BtrI CACGTC 1 cut(s) 996
BtsCI GGATG 2 cut(s) 1072, 1173
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 3 cut(s) 41, 993, 1005
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 409, 920
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 466
Cfr10I RCCGGY 4 cut(s) 265, 439, 757, 802
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 217, 271, 467, 548
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 7 cut(s) 33, 537, 600, 663, 672, 1085, 1103
DpnI GATC 5 cut(s) 134, 491, 713, 749, 1191
DpnII GATC 5 cut(s) 132, 489, 711, 747, 1189
DriI GACNNNNNGTC 1 cut(s) 979
EaeI YGGCCR 2 cut(s) 213, 955
EagI CGGCCG 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 719
Eam1105I GACNNNNNGTC 1 cut(s) 979
EarI CTCTTC 1 cut(s) 719
EclXI CGGCCG 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 364
Eco52I CGGCCG 1 cut(s) 955
Eco57I CTGAAG 3 cut(s) 191, 494, 840
Eco72I CACGTG 2 cut(s) 262, 879
Eco81I CCTNAGG 3 cut(s) 537, 600, 663
Eco88I CYCGRG 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 2 cut(s) 932, 1010
FaeI CATG 4 cut(s) 220, 274, 470, 551
FaiI YATR 9 cut(s) 218, 272, 447, 468, 549, 612, 645, 731, 1142
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 4 cut(s) 216, 270, 466, 547
FbaI TGATCA 1 cut(s) 489
Fnu4HI GCNGC 4 cut(s) 348, 669, 719, 1059
FokI GGATG 2 cut(s) 1079, 1180
Fsp4HI GCNGC 4 cut(s) 348, 669, 719, 1059
FspBI CTAG 1 cut(s) 807
GluI GCNGC 4 cut(s) 348, 669, 719, 1059
GsuI CTGGAG 3 cut(s) 340, 756, 890
HaeIII GGCC 2 cut(s) 215, 957
HgaI GACGC 1 cut(s) 184
Hin1I GRCGYC 2 cut(s) 195, 980
Hin1II CATG 4 cut(s) 220, 274, 470, 551
HincII GTYRAC 2 cut(s) 973, 1210
HindII GTYRAC 2 cut(s) 973, 1210
HindIII AAGCTT 2 cut(s) 386, 704
HinfI GANTC 6 cut(s) 398, 500, 563, 737, 786, 1105
HphI GGTGA 5 cut(s) 80, 92, 331, 785, 1018
Hpy166II GTNNAC 5 cut(s) 433, 514, 973, 991, 1210
Hpy188I TCNGA 9 cut(s) 171, 234, 403, 418, 505, 568, 580, 742, 937
Hpy188III TCNNGA 7 cut(s) 187, 467, 530, 593, 689, 818, 1109
Hpy8I GTNNAC 5 cut(s) 433, 514, 973, 991, 1210
Hpy99I CGWCG 4 cut(s) 200, 244, 985, 1102
HpyAV CCTTC 4 cut(s) 292, 815, 890, 1003
HpyCH4III ACNGT 4 cut(s) 104, 1021, 1182, 1214
HpyCH4IV ACGT 7 cut(s) 10, 261, 315, 878, 941, 980, 995
HpyCH4V TGCA 3 cut(s) 4, 330, 1171
HpyF10VI GCNNNNNNNGC 1 cut(s) 1058
HpyF3I CTNAG 7 cut(s) 33, 537, 600, 663, 672, 1085, 1103
HpySE526I ACGT 7 cut(s) 10, 261, 315, 878, 941, 980, 995
Hsp92I GRCGYC 2 cut(s) 195, 980
Hsp92II CATG 4 cut(s) 220, 274, 470, 551
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 5 cut(s) 132, 489, 711, 747, 1189
LmnI GCTCC 1 cut(s) 281
LweI GCATC 6 cut(s) 263, 339, 385, 733, 897, 1158
MaeI CTAG 1 cut(s) 807
MaeII ACGT 7 cut(s) 10, 261, 315, 878, 941, 980, 995
MaeIII GTNAC 1 cut(s) 791
MalI GATC 5 cut(s) 134, 491, 713, 749, 1191
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 132, 489, 711, 747, 1189
MboII GAAGA 6 cut(s) 184, 662, 710, 736, 940, 1213
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 41, 278
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 4 cut(s) 5, 50, 110, 138
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 5 cut(s) 407, 509, 572, 746, 1114
MmeI TCCRAC 3 cut(s) 381, 483, 546
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 7 cut(s) 80, 113, 129, 137, 141, 965, 1023
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 1052
MspR9I CCNGG 2 cut(s) 358, 1063
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 1058
NciI CCSGG 1 cut(s) 1063
NdeII GATC 5 cut(s) 132, 489, 711, 747, 1189
NlaIII CATG 4 cut(s) 220, 274, 470, 551
NlaIV GGNNCC 2 cut(s) 362, 1029
NmuCI GTSAC 1 cut(s) 791
OliI CACNNNNGTG 1 cut(s) 998
PacI TTAATTAA 1 cut(s) 141
PaeR7I CTCGAG 1 cut(s) 187
PagI TCATGA 1 cut(s) 466
PcsI WCGNNNNNNNCGW 2 cut(s) 380, 884
PfeI GAWTC 1 cut(s) 786
PkrI GCNGC 4 cut(s) 349, 670, 720, 1060
PleI GAGTC 5 cut(s) 406, 508, 571, 745, 1113
PmaCI CACGTG 2 cut(s) 262, 879
PmlI CACGTG 2 cut(s) 262, 879
PpsI GAGTC 5 cut(s) 406, 508, 571, 745, 1113
Ppu21I YACGTR 2 cut(s) 262, 879
PshAI GACNNNNGTC 3 cut(s) 684, 1052, 1180
Psp6I CCWGG 1 cut(s) 356
PspCI CACGTG 2 cut(s) 262, 879
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 362, 1029
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 998
SaqAI TTAA 7 cut(s) 80, 113, 129, 137, 141, 965, 1023
SatI GCNGC 4 cut(s) 348, 669, 719, 1059
Sau3AI GATC 5 cut(s) 132, 489, 711, 747, 1189
SchI GAGTC 5 cut(s) 407, 509, 572, 746, 1114
ScrFI CCNGG 2 cut(s) 358, 1063
SduI GDGCHC 2 cut(s) 41, 278
SfaNI GCATC 6 cut(s) 263, 339, 385, 733, 897, 1158
Sfr274I CTCGAG 1 cut(s) 187
SlaI CTCGAG 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 4 cut(s) 5, 50, 110, 138
SspMI CTAG 1 cut(s) 807
StyD4I CCNGG 2 cut(s) 356, 1061
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 4 cut(s) 104, 1021, 1182, 1214
TaiI ACGT 7 cut(s) 13, 264, 318, 881, 944, 983, 998
TaqI TCGA 4 cut(s) 188, 529, 592, 905
TaqII GACCGA 1 cut(s) 891
TasI AATT 4 cut(s) 5, 50, 110, 138
TauI GCSGC 4 cut(s) 350, 671, 721, 1061
TfiI GAWTC 1 cut(s) 786
Tru1I TTAA 7 cut(s) 80, 113, 129, 137, 141, 965, 1023
Tru9I TTAA 7 cut(s) 80, 113, 129, 137, 141, 965, 1023
TscAI CASTG 3 cut(s) 41, 993, 1005
TseFI GTSAC 1 cut(s) 791
Tsp45I GTSAC 1 cut(s) 791
TspDTI ATGAA 3 cut(s) 233, 455, 1129
TspGWI ACGGA 5 cut(s) 257, 287, 294, 871, 908
TspRI CASTG 3 cut(s) 41, 993, 1005
XhoI CTCGAG 1 cut(s) 187
XspI CTAG 1 cut(s) 807
ZraI GACGTC 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.