pycom10g25460

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011830
Physical Location & Seq
Forward (+)
27102643 .. 27104040
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25460.1

Sequence Viewer

Length: 1017 bp
ATGTCTTGGTTAGCTTCTCTGAAGATCGTCCTGATTTCGAGCGGCTTTGTGGCGTGGGCTCTGGCCATGAAGATCTCCTTTCCGATGGCGGCGGAGTTCGTAGCCTCCAACTTTTCGGTCATGTGCTCCTCATTCCGATTCTTGCTCCGCCCTCCGTACCTCTACTTCCTCGTCAACGGCATCATCATCACCATCGCCGCTTCCTCCAGATTCGACGGCTACGGCAGGTCGCCTCAGCCGAAGCTTCAACCGGAGTCGGAGGTGAGCGTTTCGGAGCCAGCGCCGGTTTACGCGCCGATGTATCGAGAGCCGAAGGTTCATAAGCTGACGTCTGCGGTTGTGATCAAACCGGGGTCGGAGGCGGTTTACGAACCGAGGTATCGAGAGCCTGAGGTTGTTCATGAGTCGACGTCCGCGTTTGTGAGCAATGGCTACGTAGAAGAAGCAAAGCCTGAGGTGGCTCAGAATAAAGATGATCGCAAATACGAAGATAAGCTTGTGATCTGGCCGCCGAAGAGTATGGCGGAGTCCGATGAGATTGCTTTGCCCGTGAAGAGACCTCTAGTTTCGGCGAGATACAGTCACCGGAAACCGGCTAGATCCAGTCCTGAAGGTGGGAGGCCTTTGAGAGTGGCGCAGCCAAAACAGTACGACACGCTGGAGAGCACGTGGAAGGCGATAACGGAAGGTCGGTCAAATACGTTGAGCAGGCGAATGGAGAAGTCCGAGACGTTCCATAACCGCGGCTGTCAGTTAAACGTTGACTTGACGTCGGCGGTGGACACGCCAGTGATGGCGAAGGAAGAGACGTTTAAGGACCGAAGCAACCAGAAACTGAGAGGTGCAGCCGCCGTGGATGGAAGTGGAGGGAGGCTGAGGAAAGAGCCGTCGCTGAGTCAGGACGAGTTGAACCGCCGAGCGGAAGAGTTCATAAACAAGTTCAATGAGGAAATGAGGCTGCAGAGACAAAAGTCTTTGAATCAGTACATGAAGATGGTCAACCCTGGAAGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

339

Amino Acids

38.14

Weight (kDa)

9.36

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 41 - 71 2.6e-13 Domain of unknown function (DUF4408)
DUF761 PF05553 300 - 334 8.9e-15 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61260
fragaria_vesca FvH4_3g04160
malus_domestica MD05G1324300.v1.1 MD10G1302100.v1.1
prunus_persica Prupe.4G035700_v2.0.a1
pyrus_communis pycom05g29850 pycom10g25460
rosa_chinensis RchiOBHm_Chr5g0006611
rosa_laevigata RLG00000031406
rosa_multiflora Rmu_co8099104.1_g000001 Rmu_sc0001972.1_g000005 Rmu_sc0011154.1_g000002
rosa_roxburghii Rroxscaffold_1G00069400
rosa_rugosa Rorug04G0423100
rosa_samantha Rh5AG050500 Rh5BG049600 Rh5CG058100 Rh5DG049000
rosa_wichuraiana Rw5G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 332, 413, 773
Acc36I ACCTGC 1 cut(s) 216
AccBSI CCGCTC 2 cut(s) 42, 920
AccI GTMKAC 1 cut(s) 407
AccII CGCG 3 cut(s) 293, 416, 744
AclI AACGTT 1 cut(s) 759
AclWI GGATC 1 cut(s) 594
AcoI YGGCCR 2 cut(s) 63, 506
AcuI CTGAAG 2 cut(s) 41, 630
AcvI CACGTG 1 cut(s) 669
AcyI GRCGYC 3 cut(s) 329, 410, 770
AfaI GTAC 3 cut(s) 158, 650, 986
AfiI CCNNNNNNNGG 3 cut(s) 592, 614, 919
AgsI TTSAA 4 cut(s) 248, 910, 943, 979
AhdI GACNNNNNGTC 1 cut(s) 769
AjnI CCWGG 1 cut(s) 1003
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 4 cut(s) 14, 244, 325, 496
AluI AGCT 4 cut(s) 14, 244, 325, 496
Alw21I GWGCWC 2 cut(s) 128, 668
Alw26I GTCTC 4 cut(s) 550, 722, 800, 958
AlwI GGATC 1 cut(s) 594
AlwNI CAGNNNCTG 1 cut(s) 835
AoxI GGCC 3 cut(s) 63, 506, 620
ApeKI GCWGC 3 cut(s) 637, 845, 958
AspLEI GCGC 3 cut(s) 283, 295, 637
AspS9I GGNCC 1 cut(s) 817
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 3 cut(s) 181, 274, 575
AvaII GGWCC 1 cut(s) 817
AxyI CCTNAGG 2 cut(s) 390, 453
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BalI TGGCCA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbrPI CACGTG 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 128, 668
BbvCI CCTCAGC 2 cut(s) 234, 875
BbvI GCAGC 3 cut(s) 649, 857, 945
BccI CCATC 5 cut(s) 79, 200, 787, 851, 988
BceAI ACGGC 5 cut(s) 193, 232, 238, 836, 871
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 1005
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 4 cut(s) 550, 722, 800, 958
BfaI CTAG 2 cut(s) 563, 597
BfmI CTRYAG 1 cut(s) 959
BfoI RGCGCY 1 cut(s) 284
BfuAI ACCTGC 1 cut(s) 216
BglII AGATCT 1 cut(s) 72
BisI GCNGC 9 cut(s) 43, 90, 198, 509, 638, 745, 846, 849, 959
BlsI GCNGC 9 cut(s) 44, 91, 199, 510, 639, 746, 847, 850, 960
Bme1390I CCNGG 2 cut(s) 351, 1005
Bme18I GGWCC 1 cut(s) 817
BmeRI GACNNNNNGTC 1 cut(s) 769
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 2 cut(s) 351, 1005
BmsI GCATC 1 cut(s) 189
BoxI GACNNNNGTC 1 cut(s) 970
BpmI CTGGAG 2 cut(s) 190, 680
Bpu10I CCTNAGC 2 cut(s) 234, 875
BpuMI CCSGG 1 cut(s) 351
BsaAI YACGTR 2 cut(s) 436, 669
BsaHI GRCGYC 3 cut(s) 329, 410, 770
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 5 cut(s) 350, 374, 742, 852, 1003
BsaWI WCCGGW 2 cut(s) 250, 585
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 3 cut(s) 592, 614, 919
Bse118I RCCGGY 2 cut(s) 283, 592
Bse1I ACTGG 2 cut(s) 603, 788
Bse21I CCTNAGG 2 cut(s) 390, 453
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 1005
BseDI CCNNGG 5 cut(s) 350, 374, 742, 852, 1003
BseGI GGATG 1 cut(s) 862
BseLI CCNNNNNNNGG 3 cut(s) 592, 614, 919
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 7 cut(s) 248, 381, 444, 476, 827, 866, 884
BseNI ACTGG 2 cut(s) 603, 788
BseRI GAGGAG 1 cut(s) 118
BseXI GCAGC 3 cut(s) 649, 857, 945
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 3 cut(s) 293, 416, 744
BshFI GGCC 3 cut(s) 65, 508, 622
BsiHKAI GWGCWC 2 cut(s) 128, 668
BsiSI CCGG 5 cut(s) 251, 284, 350, 586, 593
BslI CCNNNNNNNGG 3 cut(s) 592, 614, 919
BsmAI GTCTC 4 cut(s) 550, 722, 800, 958
BsmBI CGTCTC 2 cut(s) 722, 800
BsnI GGCC 3 cut(s) 65, 508, 622
Bso31I GGTCTC 1 cut(s) 550
Bsp1286I GDGCHC 3 cut(s) 61, 128, 668
Bsp143I GATC 6 cut(s) 24, 72, 342, 475, 501, 599
BspANI GGCC 3 cut(s) 65, 508, 622
BspCNI CTCAG 7 cut(s) 247, 382, 445, 475, 828, 867, 885
BspFNI CGCG 3 cut(s) 293, 416, 744
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 276
BspMAI CTGCAG 1 cut(s) 963
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 1 cut(s) 594
BspTNI GGTCTC 1 cut(s) 550
BsrBI CCGCTC 2 cut(s) 42, 920
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 2 cut(s) 283, 592
BsrI ACTGG 2 cut(s) 603, 788
BssAI RCCGGY 2 cut(s) 283, 592
BssECI CCNNGG 5 cut(s) 350, 374, 742, 852, 1003
BssMI GATC 6 cut(s) 24, 72, 342, 475, 501, 599
BssNI GRCGYC 3 cut(s) 329, 410, 770
Bst2UI CCWGG 1 cut(s) 1005
Bst4CI ACNGT 2 cut(s) 581, 648
Bst6I CTCTTC 4 cut(s) 509, 548, 798, 918
BstACI GRCGYC 3 cut(s) 329, 410, 770
BstBAI YACGTR 2 cut(s) 436, 669
BstC8I GCNNGC 2 cut(s) 279, 710
BstDEI CTNAG 7 cut(s) 234, 390, 453, 462, 836, 875, 893
BstDSI CCRYGG 2 cut(s) 742, 852
BstF5I GGATG 1 cut(s) 862
BstFNI CGCG 3 cut(s) 293, 416, 744
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 3 cut(s) 283, 295, 637
BstKTI GATC 6 cut(s) 27, 75, 345, 478, 504, 602
BstMAI GTCTC 4 cut(s) 550, 722, 800, 958
BstMBI GATC 6 cut(s) 24, 72, 342, 475, 501, 599
BstNI CCWGG 1 cut(s) 1005
BstPAI GACNNNNGTC 1 cut(s) 970
BstSCI CCNGG 2 cut(s) 349, 1003
BstSFI CTRYAG 1 cut(s) 959
BstSNI TACGTA 1 cut(s) 436
BstUI CGCG 3 cut(s) 293, 416, 744
BstV1I GCAGC 3 cut(s) 649, 857, 945
BstX2I RGATCY 2 cut(s) 72, 599
BstYI RGATCY 2 cut(s) 72, 599
Bsu36I CCTNAGG 2 cut(s) 390, 453
BsuRI GGCC 3 cut(s) 65, 508, 622
BtgI CCRYGG 2 cut(s) 742, 852
BtgZI GCGATG 1 cut(s) 178
BtsCI GGATG 1 cut(s) 862
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 279, 710
CaiI CAGNNNCTG 1 cut(s) 835
CciI TCATGA 1 cut(s) 400
CfoI GCGC 3 cut(s) 283, 295, 637
Cfr10I RCCGGY 2 cut(s) 283, 592
Cfr13I GGNCC 1 cut(s) 817
Cfr42I CCGCGG 1 cut(s) 745
Csp6I GTAC 3 cut(s) 157, 649, 985
CviAII CATG 4 cut(s) 67, 121, 401, 988
CviQI GTAC 3 cut(s) 157, 649, 985
DdeI CTNAG 7 cut(s) 234, 390, 453, 462, 836, 875, 893
DpnI GATC 6 cut(s) 26, 74, 344, 477, 503, 601
DpnII GATC 6 cut(s) 24, 72, 342, 475, 501, 599
DriI GACNNNNNGTC 1 cut(s) 769
EaeI YGGCCR 2 cut(s) 63, 506
Eam1104I CTCTTC 4 cut(s) 509, 548, 798, 918
Eam1105I GACNNNNNGTC 1 cut(s) 769
EarI CTCTTC 4 cut(s) 509, 548, 798, 918
EciI GGCGGA 3 cut(s) 107, 137, 539
Eco105I TACGTA 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 2 cut(s) 41, 630
Eco72I CACGTG 1 cut(s) 669
Eco81I CCTNAGG 2 cut(s) 390, 453
EcoRII CCWGG 1 cut(s) 1003
EcoT38I GRGCYC 1 cut(s) 61
Esp3I CGTCTC 2 cut(s) 722, 800
FaeI CATG 4 cut(s) 70, 124, 404, 991
FaiI YATR 8 cut(s) 68, 122, 321, 402, 521, 738, 932, 989
FalI AAGNNNNNCTT 4 cut(s) 62, 94, 480, 512
FatI CATG 4 cut(s) 66, 120, 400, 987
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 9 cut(s) 43, 90, 198, 509, 638, 745, 846, 849, 959
FokI GGATG 1 cut(s) 869
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 9 cut(s) 43, 90, 198, 509, 638, 745, 846, 849, 959
FspBI CTAG 2 cut(s) 563, 597
GlaI GCGC 3 cut(s) 282, 294, 636
GluI GCNGC 9 cut(s) 43, 90, 198, 509, 638, 745, 846, 849, 959
GsuI CTGGAG 2 cut(s) 190, 680
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 3 cut(s) 65, 508, 622
HapII CCGG 5 cut(s) 251, 284, 350, 586, 593
HhaI GCGC 3 cut(s) 283, 295, 637
Hin1I GRCGYC 3 cut(s) 329, 410, 770
Hin1II CATG 4 cut(s) 70, 124, 404, 991
Hin6I GCGC 3 cut(s) 281, 293, 635
HinP1I GCGC 3 cut(s) 281, 293, 635
HincII GTYRAC 4 cut(s) 175, 408, 763, 1000
HindII GTYRAC 4 cut(s) 175, 408, 763, 1000
HindIII AAGCTT 2 cut(s) 242, 494
HinfI GANTC 7 cut(s) 138, 210, 254, 404, 527, 895, 979
HpaII CCGG 5 cut(s) 251, 284, 350, 586, 593
HphI GGTGA 3 cut(s) 181, 274, 575
Hpy166II GTNNAC 7 cut(s) 175, 289, 367, 408, 763, 781, 1000
Hpy188I TCNGA 9 cut(s) 21, 84, 137, 259, 274, 358, 465, 532, 727
Hpy188III TCNNGA 7 cut(s) 31, 207, 305, 383, 401, 608, 899
Hpy8I GTNNAC 7 cut(s) 175, 289, 367, 408, 763, 781, 1000
Hpy99I CGWCG 4 cut(s) 218, 412, 775, 892
HpyAV CCTTC 5 cut(s) 307, 605, 667, 680, 793
HpyCH4III ACNGT 2 cut(s) 581, 648
HpyCH4IV ACGT 9 cut(s) 329, 410, 435, 668, 701, 731, 759, 770, 809
HpyCH4V TGCA 2 cut(s) 845, 961
HpyF3I CTNAG 7 cut(s) 234, 390, 453, 462, 836, 875, 893
HpySE526I ACGT 9 cut(s) 329, 410, 435, 668, 701, 731, 759, 770, 809
Hsp92I GRCGYC 3 cut(s) 329, 410, 770
Hsp92II CATG 4 cut(s) 70, 124, 404, 991
HspAI GCGC 3 cut(s) 281, 293, 635
Ksp22I TGATCA 1 cut(s) 342
KspI CCGCGG 1 cut(s) 745
Kzo9I GATC 6 cut(s) 24, 72, 342, 475, 501, 599
LmnI GCTCC 3 cut(s) 131, 150, 274
Lsp1109I GCAGC 3 cut(s) 649, 857, 945
LweI GCATC 1 cut(s) 189
MaeI CTAG 2 cut(s) 563, 597
MaeII ACGT 9 cut(s) 329, 410, 435, 668, 701, 731, 759, 770, 809
MaeIII GTNAC 1 cut(s) 581
MalI GATC 6 cut(s) 26, 74, 344, 477, 503, 601
MbiI CCGCTC 2 cut(s) 42, 920
MboI GATC 6 cut(s) 24, 72, 342, 475, 501, 599
MboII GAAGA 9 cut(s) 34, 82, 452, 500, 526, 565, 815, 935, 1003
MflI RGATCY 2 cut(s) 72, 599
MhlI GDGCHC 3 cut(s) 61, 128, 668
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 4 cut(s) 263, 413, 536, 904
MmeI TCCRAC 3 cut(s) 132, 237, 336
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 2 cut(s) 755, 813
MslI CAYNNNNRTG 2 cut(s) 788, 992
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 744
MspI CCGG 5 cut(s) 251, 284, 350, 586, 593
MspR9I CCNGG 2 cut(s) 351, 1005
MvaI CCWGG 1 cut(s) 1005
MvnI CGCG 3 cut(s) 293, 416, 744
NciI CCSGG 1 cut(s) 351
NdeII GATC 6 cut(s) 24, 72, 342, 475, 501, 599
NlaIII CATG 4 cut(s) 70, 124, 404, 991
NlaIV GGNNCC 1 cut(s) 276
NmeAIII GCCGAG 1 cut(s) 941
NmuCI GTSAC 1 cut(s) 581
OliI CACNNNNGTG 1 cut(s) 788
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 622
PcsI WCGNNNNNNNCGW 3 cut(s) 236, 413, 674
PfeI GAWTC 3 cut(s) 138, 210, 979
PkrI GCNGC 9 cut(s) 44, 91, 199, 510, 639, 746, 847, 850, 960
PleI GAGTC 4 cut(s) 262, 412, 535, 903
PmaCI CACGTG 1 cut(s) 669
PmlI CACGTG 1 cut(s) 669
PpsI GAGTC 4 cut(s) 262, 412, 535, 903
Ppu21I YACGTR 2 cut(s) 436, 669
PshAI GACNNNNGTC 1 cut(s) 970
Psp1406I AACGTT 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 1003
PspCI CACGTG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 1003
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 817
PstI CTGCAG 1 cut(s) 963
PstNI CAGNNNCTG 1 cut(s) 835
PsuI RGATCY 2 cut(s) 72, 599
RsaI GTAC 3 cut(s) 158, 650, 986
RsaNI GTAC 3 cut(s) 157, 649, 985
RseI CAYNNNNRTG 2 cut(s) 788, 992
SacII CCGCGG 1 cut(s) 745
SalI GTCGAC 1 cut(s) 406
SaqAI TTAA 2 cut(s) 755, 813
SatI GCNGC 9 cut(s) 43, 90, 198, 509, 638, 745, 846, 849, 959
Sau3AI GATC 6 cut(s) 24, 72, 342, 475, 501, 599
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 4 cut(s) 263, 413, 536, 904
ScrFI CCNGG 2 cut(s) 351, 1005
SduI GDGCHC 3 cut(s) 61, 128, 668
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 959
Sfr303I CCGCGG 1 cut(s) 745
SgrBI CCGCGG 1 cut(s) 745
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 788, 992
SnaBI TACGTA 1 cut(s) 436
SseBI AGGCCT 1 cut(s) 622
SspMI CTAG 2 cut(s) 563, 597
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 349, 1003
TaaI ACNGT 2 cut(s) 581, 648
TaiI ACGT 9 cut(s) 332, 413, 438, 671, 704, 734, 762, 773, 812
TaqI TCGA 5 cut(s) 38, 213, 304, 382, 407
TaqII GACCGA 3 cut(s) 106, 681, 834
TatI WGTACW 1 cut(s) 984
TauI GCSGC 6 cut(s) 45, 92, 200, 511, 747, 851
TfiI GAWTC 3 cut(s) 138, 210, 979
Tru1I TTAA 2 cut(s) 755, 813
Tru9I TTAA 2 cut(s) 755, 813
TscAI CASTG 1 cut(s) 795
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 3 cut(s) 637, 845, 958
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 5 cut(s) 83, 308, 389, 919, 1004
TspGWI ACGGA 2 cut(s) 144, 698
TspRI CASTG 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 2 cut(s) 563, 597
ZraI GACGTC 3 cut(s) 330, 411, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.