MD05G1343600.v1.1

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011158
Physical Location & Seq
Reverse (-)
46357102 .. 46359379
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1343600.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGCTCAGGCAGTCGAAGAATGGTACAAGCAGATGCCTGTGATCACCCGCTCTTACCTCACCGCAGCTGTTGTCACCAACATCGGTTGCTCCCTCGACATAATCTCTCCTCACAATCTGTACTTGAACCCTAATCTAGTGGTCAAGCAGTATCAGTTCTGGCGCCTCGTTACTAATTTTCTTTACTTCCGGAAAATGGACTTGGATTTCATGTTCCACATGTTCTTTCTTGCTCGGTACTGCAAGCTTCTCGAAGAGAACTCTTTCCGAGGACGGACTGCTGATTTCTTTTACATGCTCTTATTTGGTGCCGCTGTGTTGACTGGGATAGTTCTGGTTGGAGGGATGATACCTCAATTGTCTGCGTCGTTTGCAAAGATTATATTCCTCAGCAACTCGCTAACATTCATGATGGTCTACGTGTGGAGCAAGCAAAATCCATTCATTCATATGAGCTTCTTGGGCCTCTTTACCTTCACAGCAGCTTACCTACCTTGGGTTCTTCTGACGTTCTCTGTCTTTGTTGGAGCTAGCCCGTGGGTGGATCTTTTGGGAATGATTGCCGGTCATGCCTATTACTTTCTGGAAGATGTGTACCCACGAATGACAGGTCGCCGGCCCTTGAGAACCCCTTCTTTTATCAAAGCACTGTTTGCGGATGACCCTGTTGTGGTGGCACGACCAGGAAATGTGAGGTTTGCACCACCACCCGTGGAGGAACTTCACCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

245

Amino Acids

28.08

Weight (kDa)

8.83

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 12 - 212 8.5e-62 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 162, 308
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 417
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 4 cut(s) 49, 63, 312, 656
AclWI GGATC 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 4 cut(s) 26, 122, 239, 596
AfiI CCNNNNNNNGG 4 cut(s) 196, 496, 541, 670
AflIII ACRYGT 2 cut(s) 219, 420
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 68, 247, 456, 485, 530
AluI AGCT 5 cut(s) 68, 247, 456, 485, 530
AlwI GGATC 1 cut(s) 552
Aor13HI TCCGGA 1 cut(s) 189
AoxI GGCC 2 cut(s) 463, 617
ApeKI GCWGC 2 cut(s) 65, 482
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
Asp700I GAANNNNTTC 2 cut(s) 263, 631
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 463, 618
AsuHPI GGTGA 4 cut(s) 37, 52, 67, 716
AsuNHI GCTAGC 1 cut(s) 530
BanI GGYRCC 2 cut(s) 162, 308
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 2 cut(s) 77, 494
BccI CCATC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 1 cut(s) 684
BclI TGATCA 1 cut(s) 42
BfaI CTAG 2 cut(s) 137, 531
BfoI RGCGCY 1 cut(s) 166
BisI GCNGC 3 cut(s) 66, 312, 483
BlsI GCNGC 3 cut(s) 67, 313, 484
Bme1390I CCNGG 1 cut(s) 684
BmgT120I GGNCC 2 cut(s) 463, 618
BmiI GGNNCC 2 cut(s) 164, 310
BmrFI CCNGG 1 cut(s) 684
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 24
BmtI GCTAGC 1 cut(s) 534
BmuI ACTGGG 1 cut(s) 333
Bpu10I CCTNAGC 2 cut(s) 6, 389
BpuEI CTTGAG 1 cut(s) 643
BsaAI YACGTR 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 4 cut(s) 268, 494, 536, 711
BsaWI WCCGGW 1 cut(s) 189
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 496, 541, 670
Bse118I RCCGGY 2 cut(s) 563, 615
Bse1I ACTGG 1 cut(s) 328
BseAI TCCGGA 1 cut(s) 189
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 4 cut(s) 268, 494, 536, 711
BseGI GGATG 2 cut(s) 351, 664
BseLI CCNNNNNNNGG 4 cut(s) 196, 496, 541, 670
BseMII CTCAG 2 cut(s) 20, 403
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 77, 494
BshFI GGCC 2 cut(s) 465, 619
BshNI GGYRCC 2 cut(s) 162, 308
BsiSI CCGG 3 cut(s) 190, 564, 616
BslI CCNNNNNNNGG 4 cut(s) 196, 496, 541, 670
BsnI GGCC 2 cut(s) 465, 619
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 42, 544
BspACI CCGC 4 cut(s) 49, 63, 312, 656
BspANI GGCC 2 cut(s) 465, 619
BspCNI CTCAG 2 cut(s) 19, 402
BspEI TCCGGA 1 cut(s) 189
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 164, 310
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 1 cut(s) 552
BspT107I GGYRCC 2 cut(s) 162, 308
BsrBI CCGCTC 1 cut(s) 51
BsrFI RCCGGY 2 cut(s) 563, 615
BsrI ACTGG 1 cut(s) 328
BssAI RCCGGY 2 cut(s) 563, 615
BssECI CCNNGG 4 cut(s) 268, 494, 536, 711
BssMI GATC 2 cut(s) 42, 544
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 1 cut(s) 651
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 4 cut(s) 245, 431, 532, 617
BstDEI CTNAG 2 cut(s) 6, 389
BstDSI CCRYGG 2 cut(s) 536, 711
BstF5I GGATG 2 cut(s) 351, 664
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 2 cut(s) 45, 547
BstMBI GATC 2 cut(s) 42, 544
BstMWI GCNNNNNNNGC 4 cut(s) 371, 462, 569, 653
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 2 cut(s) 223, 298
BstSCI CCNGG 1 cut(s) 682
BstV1I GCAGC 2 cut(s) 77, 494
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BsuRI GGCC 2 cut(s) 465, 619
BtgI CCRYGG 2 cut(s) 536, 711
BtsCI GGATG 2 cut(s) 351, 664
BtsIMutI CAGTG 1 cut(s) 647
Cac8I GCNNGC 4 cut(s) 245, 431, 532, 617
CciI TCATGA 1 cut(s) 408
CfoI GCGC 1 cut(s) 165
Cfr10I RCCGGY 2 cut(s) 563, 615
Cfr13I GGNCC 2 cut(s) 463, 618
CseI GACGC 1 cut(s) 354
Csp6I GTAC 4 cut(s) 25, 121, 238, 595
CviAII CATG 5 cut(s) 211, 220, 295, 409, 569
CviJI RGCY 9 cut(s) 5, 68, 247, 456, 465, 485, 530, 534, 619
CviKI_1 RGCY 9 cut(s) 5, 68, 247, 456, 465, 485, 530, 534, 619
CviQI GTAC 4 cut(s) 25, 121, 238, 595
DdeI CTNAG 2 cut(s) 6, 389
DinI GGCGCC 1 cut(s) 164
DpnI GATC 2 cut(s) 44, 546
DpnII GATC 2 cut(s) 42, 544
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 494
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 5 cut(s) 214, 223, 298, 412, 572
FaiI YATR 9 cut(s) 101, 212, 221, 296, 383, 410, 450, 452, 570
FatI CATG 5 cut(s) 210, 219, 294, 408, 568
FauI CCCGC 1 cut(s) 56
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 66, 312, 483
FokI GGATG 2 cut(s) 358, 671
Fsp4HI GCNGC 3 cut(s) 66, 312, 483
FspBI CTAG 2 cut(s) 137, 531
GlaI GCGC 1 cut(s) 164
GluI GCNGC 3 cut(s) 66, 312, 483
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 2 cut(s) 465, 619
HapII CCGG 3 cut(s) 190, 564, 616
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 5 cut(s) 214, 223, 298, 412, 572
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 245
HpaII CCGG 3 cut(s) 190, 564, 616
HphI GGTGA 4 cut(s) 37, 52, 67, 716
Hpy166II GTNNAC 3 cut(s) 321, 418, 595
Hpy188I TCNGA 2 cut(s) 269, 507
Hpy188III TCNNGA 4 cut(s) 190, 251, 409, 584
Hpy8I GTNNAC 3 cut(s) 321, 418, 595
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 2 cut(s) 484, 642
HpyCH4III ACNGT 1 cut(s) 651
HpyCH4IV ACGT 2 cut(s) 420, 509
HpyCH4V TGCA 3 cut(s) 243, 374, 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 371, 462, 569, 653
HpyF3I CTNAG 2 cut(s) 6, 389
HpySE526I ACGT 2 cut(s) 420, 509
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 5 cut(s) 214, 223, 298, 412, 572
HspAI GCGC 1 cut(s) 163
KasI GGCGCC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 189
KroI GCCGGC 1 cut(s) 615
KroNI GCCGGC 1 cut(s) 617
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 2 cut(s) 42, 544
LmnI GCTCC 3 cut(s) 95, 426, 527
Lsp1109I GCAGC 2 cut(s) 77, 494
LweI GCATC 1 cut(s) 24
MaeI CTAG 2 cut(s) 137, 531
MaeII ACGT 2 cut(s) 420, 509
MaeIII GTNAC 2 cut(s) 73, 169
MalI GATC 2 cut(s) 44, 546
MbiI CCGCTC 1 cut(s) 51
MboI GATC 2 cut(s) 42, 544
MboII GAAGA 4 cut(s) 29, 266, 494, 599
MfeI CAATTG 1 cut(s) 356
MflI RGATCY 1 cut(s) 544
MluCI AATT 2 cut(s) 175, 356
Mly113I GGCGCC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 319, 505
MroI TCCGGA 1 cut(s) 189
MroNI GCCGGC 1 cut(s) 615
MroXI GAANNNNTTC 2 cut(s) 263, 631
MseI TTAA 1 cut(s) 733
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 68, 314
MspI CCGG 3 cut(s) 190, 564, 616
MspR9I CCNGG 1 cut(s) 684
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 4 cut(s) 371, 462, 569, 653
NaeI GCCGGC 1 cut(s) 617
NarI GGCGCC 1 cut(s) 163
NdeI CATATG 1 cut(s) 450
NdeII GATC 2 cut(s) 42, 544
NgoMIV GCCGGC 1 cut(s) 615
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 5 cut(s) 214, 223, 298, 412, 572
NlaIV GGNNCC 2 cut(s) 164, 310
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 2 cut(s) 223, 298
PagI TCATGA 1 cut(s) 408
PciI ACATGT 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 617
PdmI GAANNNNTTC 2 cut(s) 263, 631
PkrI GCNGC 3 cut(s) 67, 313, 484
PluTI GGCGCC 1 cut(s) 166
Ppu21I YACGTR 1 cut(s) 421
PscI ACATGT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 2 cut(s) 164, 310
PspPI GGNCC 2 cut(s) 463, 618
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 4 cut(s) 26, 122, 239, 596
RsaNI GTAC 4 cut(s) 25, 121, 238, 595
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 3 cut(s) 66, 312, 483
Sau3AI GATC 2 cut(s) 42, 544
Sau96I GGNCC 2 cut(s) 463, 618
ScrFI CCNGG 1 cut(s) 684
SfaNI GCATC 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 2 cut(s) 175, 356
SsiI CCGC 4 cut(s) 49, 63, 312, 656
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 2 cut(s) 137, 531
StyD4I CCNGG 1 cut(s) 682
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 1 cut(s) 651
TaiI ACGT 2 cut(s) 423, 512
TaqI TCGA 3 cut(s) 15, 96, 252
TasI AATT 2 cut(s) 175, 356
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TscAI CASTG 1 cut(s) 654
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 65, 482
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 4 cut(s) 199, 397, 433, 437
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 1 cut(s) 654
XceI RCATGY 2 cut(s) 223, 298
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 2 cut(s) 263, 631
XspI CTAG 2 cut(s) 137, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.