Rh5AG031500

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011158
Physical Location & Seq
Forward (+)
2193257 .. 2195619
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG031500.1

Sequence Viewer

Length: 570 bp
ATGGCTCAAGCAGTTGAAGAATGGTACAAGCAGATGCCGGTGATCACCAGATGGTATCTCACCGCCGCTGTCGTCACCAGTATCGGTTGCTCCCTCGATATAATCTCGCCTCACAATCTGTACTTGAACCCTATACTAGTGGTTAAGCAGTATCAACTATGGCGCCTCATCACTAATTTCCTGTACTTTCGAAAGATAGACTTGGATTTTCTGTTCCACATGTTCTTCCTTGCTCGGTACTGCAAGCTTCTTGAAGAGAACTCATTCAGGGGAAGGACGGCTGATTTCTTTTACATGCTCTTATTCGGTGCGTCTGTGTTGACTGGGATAGTTCTGGTTGGAGGAATGATACCTTATTTGTCAGAGTCATTTGCAAAGATTATATTCCTTAGCAACTCGCTAACATTCATGATGGTCTATGTGTGGAGCAAGCAAAACCCTTTCATCCATATGAGTTTCTTGGGACTTTTTAATTTCACCGCAGCCTACCTACCATGGTTTCTTCTAGGCTTCTCTGTCCTTGTCGGGGCAAGCGCTTGGGTGGATCTCCTGGTATGTTATTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

189

Amino Acids

22.08

Weight (kDa)

8.4

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 12 - 184 1.9e-50 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AciI CCGC 3 cut(s) 63, 66, 480
AclWI GGATC 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 4 cut(s) 26, 122, 185, 239
AfeI AGCGCT 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 526
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 3 cut(s) 17, 127, 254
AhlI ACTAGT 1 cut(s) 136
AjnI CCWGG 1 cut(s) 549
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 1 cut(s) 247
AluI AGCT 1 cut(s) 247
AlwI GGATC 1 cut(s) 552
Aor51HI AGCGCT 1 cut(s) 535
ApeKI GCWGC 1 cut(s) 482
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 165, 536
AsuHPI GGTGA 5 cut(s) 37, 52, 52, 67, 469
AsuII TTCGAA 1 cut(s) 190
BanI GGYRCC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 494
BccI CCATC 2 cut(s) 45, 406
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 551
BclI TGATCA 1 cut(s) 42
BcuI ACTAGT 1 cut(s) 136
BfaI CTAG 2 cut(s) 137, 506
BfoI RGCGCY 2 cut(s) 166, 537
BisI GCNGC 2 cut(s) 66, 483
BlsI GCNGC 2 cut(s) 67, 484
Bme1390I CCNGG 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 551
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 78, 328
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 526
BseNI ACTGG 2 cut(s) 78, 328
BseXI GCAGC 1 cut(s) 494
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 477
Bsp119I TTCGAA 1 cut(s) 190
Bsp143I GATC 2 cut(s) 42, 544
Bsp19I CCATGG 1 cut(s) 494
BspACI CCGC 3 cut(s) 63, 66, 480
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 190
BspT107I GGYRCC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 2 cut(s) 78, 328
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 2 cut(s) 42, 544
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 245, 431, 532
BstDEI CTNAG 1 cut(s) 389
BstDSI CCRYGG 1 cut(s) 494
BstF5I GGATG 1 cut(s) 444
BstH2I RGCGCY 2 cut(s) 166, 537
BstHHI GCGC 2 cut(s) 165, 536
BstKTI GATC 2 cut(s) 45, 547
BstMBI GATC 2 cut(s) 42, 544
BstNI CCWGG 1 cut(s) 551
BstNSI RCATGY 2 cut(s) 223, 298
BstSCI CCNGG 1 cut(s) 549
BstV1I GCAGC 1 cut(s) 494
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BtgI CCRYGG 1 cut(s) 494
BtsCI GGATG 1 cut(s) 444
Cac8I GCNNGC 3 cut(s) 245, 431, 532
CciI TCATGA 1 cut(s) 408
CfoI GCGC 2 cut(s) 165, 536
Cfr10I RCCGGY 1 cut(s) 37
CseI GACGC 1 cut(s) 300
Csp6I GTAC 4 cut(s) 25, 121, 184, 238
CviAII CATG 4 cut(s) 220, 295, 409, 495
CviJI RGCY 5 cut(s) 5, 247, 281, 485, 510
CviKI_1 RGCY 5 cut(s) 5, 247, 281, 485, 510
CviQI GTAC 4 cut(s) 25, 121, 184, 238
DdeI CTNAG 1 cut(s) 389
DinI GGCGCC 1 cut(s) 164
DpnI GATC 2 cut(s) 44, 546
DpnII GATC 2 cut(s) 42, 544
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 494
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 4 cut(s) 223, 298, 412, 498
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 1 cut(s) 477
FatI CATG 4 cut(s) 219, 294, 408, 494
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 66, 483
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 66, 483
FspBI CTAG 2 cut(s) 137, 506
GlaI GCGC 2 cut(s) 164, 535
GluI GCNGC 2 cut(s) 66, 483
HaeII RGCGCY 2 cut(s) 166, 537
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 300
HhaI GCGC 2 cut(s) 165, 536
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 4 cut(s) 223, 298, 412, 498
Hin6I GCGC 2 cut(s) 163, 534
HinP1I GCGC 2 cut(s) 163, 534
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 38
HphI GGTGA 5 cut(s) 37, 52, 52, 67, 469
Hpy166II GTNNAC 1 cut(s) 321
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 2 cut(s) 251, 409
Hpy8I GTNNAC 1 cut(s) 321
HpyAV CCTTC 1 cut(s) 267
HpyCH4V TGCA 2 cut(s) 243, 374
HpyF3I CTNAG 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 4 cut(s) 223, 298, 412, 498
HspAI GCGC 2 cut(s) 163, 534
KasI GGCGCC 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 2 cut(s) 42, 544
LmnI GCTCC 2 cut(s) 95, 426
LpnPI CCDG 9 cut(s) 51, 61, 91, 194, 253, 309, 320, 536, 563
Lsp1109I GCAGC 1 cut(s) 494
LweI GCATC 1 cut(s) 24
MaeI CTAG 2 cut(s) 137, 506
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 44, 546
MboI GATC 2 cut(s) 42, 544
MboII GAAGA 4 cut(s) 29, 217, 266, 494
MflI RGATCY 1 cut(s) 544
MluCI AATT 2 cut(s) 175, 472
Mly113I GGCGCC 1 cut(s) 163
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 4 cut(s) 104, 120, 176, 335
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 3 cut(s) 144, 471, 568
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 551
NarI GGCGCC 1 cut(s) 163
NcoI CCATGG 1 cut(s) 494
NdeI CATATG 1 cut(s) 450
NdeII GATC 2 cut(s) 42, 544
NlaIII CATG 4 cut(s) 223, 298, 412, 498
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 2 cut(s) 223, 298
NspV TTCGAA 1 cut(s) 190
PagI TCATGA 1 cut(s) 408
PciI ACATGT 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 67, 484
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 373
PscI ACATGT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 544
RsaI GTAC 4 cut(s) 26, 122, 185, 239
RsaNI GTAC 4 cut(s) 25, 121, 184, 238
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 3 cut(s) 144, 471, 568
SatI GCNGC 2 cut(s) 66, 483
Sau3AI GATC 2 cut(s) 42, 544
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 551
SetI ASST 3 cut(s) 249, 355, 492
SfaNI GCATC 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SpeI ACTAGT 1 cut(s) 136
Sse9I AATT 2 cut(s) 175, 472
SsiI CCGC 3 cut(s) 63, 66, 480
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 2 cut(s) 137, 506
StyD4I CCNGG 1 cut(s) 549
StyI CCWWGG 1 cut(s) 494
TaqI TCGA 2 cut(s) 96, 190
TasI AATT 2 cut(s) 175, 472
TatI WGTACW 2 cut(s) 120, 183
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 144, 471, 568
Tru9I TTAA 3 cut(s) 144, 471, 568
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 397, 433
XceI RCATGY 2 cut(s) 223, 298
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 137, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.