Rorug04G0404600

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011158
Physical Location & Seq
Forward (+)
56891371 .. 56892355
985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0404600.1

Sequence Viewer

Length: 720 bp
ATGATTGTGAATCGTCAAACTAGTAATAATGACGACTTGTATGGAGTAATTGTGAATAGTGAGGAAGAAGCATACAACCTCTACTGTGAGTATGGTGCTAGAATGGGGTTTAGTGTTCGGATAATGCAGAGGAGGAAAAGCAACAACGTTGTGAGACAAGTTAATTATGCATGTTCAAAAGAAGATTTTAAACTAGATAGTGATCCTTCAGAAGTGAAAAAGGCTGATAGGTTGGACACTAGAACTGGCTGCACAGCAAAAATTCGATTTGGATTGCAAGATAATAATGTATGGAAAGCAACTCTCTTTGTTCCTGAACACAACCACAAGCTTGCAGAGCCAGAAGAAAGACCATTTTTGCGCTCCAATCGAAAAGTGTTAGAAGCTCATAAGGGGGTGATTAGATCAATGAAAAACGCAGAAGAAGTTGGGGGATCTCAAAATGTTGGATTTACAAAGACTGATTGTTACAATTTTGTAAGCAGAGAGAGAATGATTATGCTAGAAGCAGGGGATGCTCAAAGCTTGATCAATTTATTTAAGAAAAAGCAAATTGAAGATCCTATGTTTTTCTACACCGTGCAACGAAATAACATAACCACATCATACTTGGGGTATGGTGCTAGCCATTTATCTGGCTTCTTGAACTCAATGTTTGTTGGACTGCAGGCTAATGCTTCTTCTAGACTCTCAGCTTCAAAACTTTCTGACAACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

239

Amino Acids

27.18

Weight (kDa)

8.96

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 27 - 112 1.1e-19 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 147
AclWI GGATC 3 cut(s) 197, 442, 554
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 192
AgsI TTSAA 4 cut(s) 177, 557, 646, 699
AhlI ACTAGT 1 cut(s) 20
AluBI AGCT 4 cut(s) 331, 386, 525, 695
AluI AGCT 4 cut(s) 331, 386, 525, 695
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 3 cut(s) 197, 442, 554
ApeKI GCWGC 1 cut(s) 249
ApoI RAATTY 1 cut(s) 261
AspLEI GCGC 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 409
AsuNHI GCTAGC 1 cut(s) 623
BbvI GCAGC 1 cut(s) 236
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 148
BcuI ACTAGT 1 cut(s) 20
BfaI CTAG 7 cut(s) 21, 99, 194, 240, 503, 624, 684
BfmI CTRYAG 1 cut(s) 665
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
BmsI GCATC 1 cut(s) 505
BmtI GCTAGC 1 cut(s) 627
BsaBI GATNNNNATC 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bse1I ACTGG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 201
BseGI GGATG 1 cut(s) 520
BseJI GATNNNNATC 1 cut(s) 201
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 1 cut(s) 235
BsmAI GTCTC 1 cut(s) 148
Bsp143I GATC 5 cut(s) 202, 404, 434, 528, 559
BspCNI CTCAG 1 cut(s) 704
BspMAI CTGCAG 1 cut(s) 669
BspOI GCTAGC 1 cut(s) 627
BspPI GGATC 3 cut(s) 197, 442, 554
BsrI ACTGG 1 cut(s) 250
BssMI GATC 5 cut(s) 202, 404, 434, 528, 559
Bst4CI ACNGT 2 cut(s) 86, 580
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 333, 625, 669
BstDEI CTNAG 2 cut(s) 691, 717
BstF5I GGATG 1 cut(s) 520
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 5 cut(s) 205, 407, 437, 531, 562
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 5 cut(s) 202, 404, 434, 528, 559
BstMWI GCNNNNNNNGC 2 cut(s) 337, 515
BstNSI RCATGY 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 665
BstV1I GCAGC 1 cut(s) 236
BstX2I RGATCY 2 cut(s) 434, 559
BstXI CCANNNNNNTGG 1 cut(s) 635
BstYI RGATCY 2 cut(s) 434, 559
BtsCI GGATG 1 cut(s) 520
Cac8I GCNNGC 3 cut(s) 333, 625, 669
CfoI GCGC 1 cut(s) 363
CviAII CATG 1 cut(s) 171
DdeI CTNAG 2 cut(s) 691, 717
DpnI GATC 5 cut(s) 204, 406, 436, 530, 561
DpnII GATC 5 cut(s) 202, 404, 434, 528, 559
DraI TTTAAA 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 172
FaeI CATG 1 cut(s) 174
FatI CATG 1 cut(s) 170
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 7 cut(s) 21, 99, 194, 240, 503, 624, 684
GlaI GCGC 1 cut(s) 362
GluI GCNGC 1 cut(s) 250
HhaI GCGC 1 cut(s) 363
Hin1II CATG 1 cut(s) 174
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 329, 523
HinfI GANTC 2 cut(s) 10, 687
HphI GGTGA 1 cut(s) 409
Hpy188I TCNGA 3 cut(s) 120, 211, 709
Hpy188III TCNNGA 3 cut(s) 314, 643, 684
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 2 cut(s) 86, 580
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 7 cut(s) 127, 170, 252, 277, 335, 583, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 515
HpyF3I CTNAG 2 cut(s) 691, 717
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 174
HspAI GCGC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 5 cut(s) 202, 404, 434, 528, 559
LmnI GCTCC 1 cut(s) 368
LpnPI CCDG 6 cut(s) 231, 327, 354, 495, 621, 653
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 505
MaeI CTAG 7 cut(s) 21, 99, 194, 240, 503, 624, 684
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 467
MalI GATC 5 cut(s) 204, 406, 436, 530, 561
MboI GATC 5 cut(s) 202, 404, 434, 528, 559
MboII GAAGA 6 cut(s) 77, 194, 356, 434, 569, 672
MflI RGATCY 2 cut(s) 434, 559
MluCI AATT 6 cut(s) 48, 163, 261, 472, 532, 552
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 3 cut(s) 213, 427, 640
MnlI CCTC 4 cut(s) 55, 89, 123, 126
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 3 cut(s) 162, 189, 540
MwoI GCNNNNNNNGC 2 cut(s) 337, 515
NdeII GATC 5 cut(s) 202, 404, 434, 528, 559
NheI GCTAGC 1 cut(s) 623
NlaIII CATG 1 cut(s) 174
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 251
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 147
PstI CTGCAG 1 cut(s) 669
PsuI RGATCY 2 cut(s) 434, 559
SaqAI TTAA 3 cut(s) 162, 189, 540
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 5 cut(s) 202, 404, 434, 528, 559
SchI GAGTC 1 cut(s) 681
SetI ASST 7 cut(s) 81, 150, 233, 333, 388, 527, 697
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 665
SpeI ACTAGT 1 cut(s) 20
Sse9I AATT 6 cut(s) 48, 163, 261, 472, 532, 552
SspMI CTAG 7 cut(s) 21, 99, 194, 240, 503, 624, 684
TaaI ACNGT 2 cut(s) 86, 580
TaiI ACGT 1 cut(s) 150
TaqI TCGA 2 cut(s) 265, 370
TasI AATT 6 cut(s) 48, 163, 261, 472, 532, 552
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 3 cut(s) 162, 189, 540
Tru9I TTAA 3 cut(s) 162, 189, 540
TseI GCWGC 1 cut(s) 249
TspDTI ATGAA 1 cut(s) 425
XapI RAATTY 1 cut(s) 261
XbaI TCTAGA 1 cut(s) 683
XceI RCATGY 1 cut(s) 174
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XspI CTAG 7 cut(s) 21, 99, 194, 240, 503, 624, 684
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.