MD05G1349300.v1.1

Belongs to the peroxisomal membrane protein PXMP2 4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011859
Physical Location & Seq
Forward (+)
46731197 .. 46733787
2591 bp
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UTR
Exon/CDS
Intron
MD05G1349300.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGGGTCGATTGCAAAGAAGGGGTTGCAGCAGTACTTGCTTCAGCTTCAGCATCATCCGCTCAGAACCAAGGCAATTACAGCGGGCGTTTTGTCGGCCTTCAGTGACATAGTGTCTCAGAAGCTCACTGGCATTCAGAAACTTCAGATAAGACGGCTTCTTCTCAAAGTGCTTCTTGGAGCTGTTTATCTTGGACCATTTGGGCATTTCCTGCATACAATACTAGATAAGATTTTCAAGGGGAAGAAGGATTCAAAAACAGTAGCAAAGAAGGTATTGGTGGAGCAGCTGACGGCTTCTCCATGGAATAACTTGGTTTTCATGCTTTACTATGGCTTGGTTGTGGAGGGAAGACCTTGGGTGCAAGTGAAAAGTAAAATTAAGTCGGACTACCCAAAAGTGCAATACACATCGTGGACGTTCTGGCCTGTTCTGGGGTGGATAAATCACCAGTATGTGCCACTTCACCTCCGCGTCATCTTCCACAGCTTGGTCGCTTTTGGCTGGGGAATTTTCCTAAATTTACAGGCAAGATCTGTGGCACTACCAAAGGCCAAGTGA

Protein Analysis

187

Amino Acids

21.17

Weight (kDa)

10.34

Isoelectric Point (pI)

27.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpv17_PMP22 PF04117 114 - 174 1.5e-21 Mpv17 / PMP22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04470 AT4G04470
fragaria_vesca FvH4_3g01800 FvH4_3g01800
malus_domestica MD05G1349300.v1.1 MD10G1323900.v1.1
prunus_persica Prupe.4G018300_v2.0.a1 Prupe.4G018300_v2.0.a1
pyrus_communis pycom05g31690 pycom10g27430
rosa_chinensis RchiOBHm_Chr5g0002631
rosa_laevigata RLG00000031048
rosa_multiflora Rmu_sc0009084.1_g000010
rosa_roxburghii Rroxscaffold_1G00073230
rosa_rugosa Rorug04G0397900
rosa_samantha Rh5AG021300 Rh5CG023400 Rh5DG022400
rosa_wichuraiana Rw5G001970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 490
AccBSI CCGCTC 1 cut(s) 61
AccII CGCG 1 cut(s) 474
AciI CCGC 3 cut(s) 59, 83, 472
AcsI RAATTY 2 cut(s) 510, 520
AcuI CTGAAG 4 cut(s) 26, 32, 85, 128
AdeI CACNNNGTG 1 cut(s) 414
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 2 cut(s) 434, 490
AgsI TTSAA 2 cut(s) 238, 255
AhdI GACNNNNNGTC 1 cut(s) 112
AluBI AGCT 5 cut(s) 46, 124, 182, 289, 489
AluI AGCT 5 cut(s) 46, 124, 182, 289, 489
Alw26I GTCTC 1 cut(s) 120
AoxI GGCC 3 cut(s) 96, 425, 552
ApeKI GCWGC 2 cut(s) 28, 286
ApoI RAATTY 2 cut(s) 510, 520
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 440, 458
AvaII GGWCC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 358
BbvI GCAGC 2 cut(s) 40, 298
BceAI ACGGC 2 cut(s) 170, 309
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 1 cut(s) 224
BglII AGATCT 1 cut(s) 533
BisI GCNGC 2 cut(s) 29, 287
BlsI GCNGC 2 cut(s) 30, 288
BmcAI AGTACT 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 194
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 194
BmsI GCATC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 69, 302, 356
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 453, 483
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 490
Bse1I ACTGG 2 cut(s) 133, 451
BseDI CCNNGG 3 cut(s) 69, 302, 356
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 434, 490
BseMII CTCAG 2 cut(s) 76, 131
BseNI ACTGG 2 cut(s) 133, 451
BseXI GCAGC 2 cut(s) 40, 298
BseYI CCCAGC 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 98, 427, 554
BslI CCNNNNNNNGG 2 cut(s) 434, 490
BsmAI GTCTC 1 cut(s) 120
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 3 cut(s) 98, 427, 554
Bsp143I GATC 1 cut(s) 533
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 3 cut(s) 59, 83, 472
BspANI GGCC 3 cut(s) 98, 427, 554
BspCNI CTCAG 2 cut(s) 75, 130
BspFNI CGCG 1 cut(s) 474
BsrBI CCGCTC 1 cut(s) 61
BsrI ACTGG 2 cut(s) 133, 451
BssECI CCNNGG 3 cut(s) 69, 302, 356
BssMI GATC 1 cut(s) 533
BssT1I CCWWGG 3 cut(s) 69, 302, 356
Bst4CI ACNGT 1 cut(s) 262
BstAPI GCANNNNNTGC 2 cut(s) 37, 211
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 62, 117
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 1 cut(s) 536
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 1 cut(s) 533
BstMWI GCNNNNNNNGC 4 cut(s) 37, 58, 80, 211
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 2 cut(s) 40, 298
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
BsuRI GGCC 3 cut(s) 98, 427, 554
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 109, 126
Cac8I GCNNGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 1 cut(s) 463
Csp6I GTAC 1 cut(s) 34
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 2 cut(s) 303, 322
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 62, 117
DpnI GATC 1 cut(s) 535
DpnII GATC 1 cut(s) 533
DraIII CACNNNGTG 1 cut(s) 414
DriI GACNNNNNGTC 1 cut(s) 112
Eam1105I GACNNNNNGTC 1 cut(s) 112
Eco130I CCWWGG 3 cut(s) 69, 302, 356
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 4 cut(s) 26, 32, 85, 128
EcoT14I CCWWGG 3 cut(s) 69, 302, 356
ErhI CCWWGG 3 cut(s) 69, 302, 356
FaeI CATG 2 cut(s) 306, 325
FaiI YATR 6 cut(s) 110, 216, 304, 323, 333, 456
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 2 cut(s) 302, 321
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 29, 287
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 29, 287
FspBI CTAG 1 cut(s) 224
GluI GCNGC 2 cut(s) 29, 287
GsaI CCCAGC 1 cut(s) 508
HaeIII GGCC 3 cut(s) 98, 427, 554
HgaI GACGC 1 cut(s) 463
Hin1II CATG 2 cut(s) 306, 325
HinfI GANTC 1 cut(s) 251
HphI GGTGA 2 cut(s) 440, 458
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 5 cut(s) 65, 120, 138, 147, 388
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 4 cut(s) 13, 109, 241, 265
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 14, 28, 214, 364, 403
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 58, 80, 211
HpyF3I CTNAG 2 cut(s) 62, 117
HpySE526I ACGT 1 cut(s) 419
Hsp92II CATG 2 cut(s) 306, 325
Kzo9I GATC 1 cut(s) 533
LmnI GCTCC 2 cut(s) 179, 283
LpnPI CCDG 8 cut(s) 114, 224, 409, 419, 441, 464, 490, 512
Lsp1109I GCAGC 2 cut(s) 40, 298
LweI GCATC 1 cut(s) 61
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 535
MbiI CCGCTC 1 cut(s) 61
MboI GATC 1 cut(s) 533
MboII GAAGA 4 cut(s) 152, 256, 363, 472
MflI RGATCY 1 cut(s) 533
MluCI AATT 4 cut(s) 75, 378, 510, 520
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 2 cut(s) 340, 479
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 453
MspA1I CMGCKG 2 cut(s) 83, 289
Mva1269I GAATGC 1 cut(s) 132
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 4 cut(s) 37, 58, 80, 211
NcoI CCATGG 1 cut(s) 302
NdeII GATC 1 cut(s) 533
NlaIII CATG 2 cut(s) 306, 325
NmuCI GTSAC 1 cut(s) 104
PctI GAATGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 2 cut(s) 30, 288
PspFI CCCAGC 1 cut(s) 504
PspPI GGNCC 1 cut(s) 194
PsuI RGATCY 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 453
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 29, 287
Sau3AI GATC 1 cut(s) 533
Sau96I GGNCC 1 cut(s) 194
ScaI AGTACT 1 cut(s) 35
SetI ASST 9 cut(s) 48, 126, 184, 276, 291, 358, 422, 471, 491
SfaNI GCATC 1 cut(s) 61
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 453
Sse9I AATT 4 cut(s) 75, 378, 510, 520
SsiI CCGC 3 cut(s) 59, 83, 472
SspMI CTAG 1 cut(s) 224
StyI CCWWGG 3 cut(s) 69, 302, 356
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 1 cut(s) 422
TaqI TCGA 1 cut(s) 8
TasI AATT 4 cut(s) 75, 378, 510, 520
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 109, 133
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 2 cut(s) 28, 286
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 310
TspRI CASTG 2 cut(s) 109, 133
Van91I CCANNNNNTGG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 2 cut(s) 510, 520
XspI CTAG 1 cut(s) 224
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.