MD05G1349400.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014683
Physical Location & Seq
Forward (+)
46736118 .. 46737389
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1349400.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGCAACGTTTCAAGCTTCAAGAATCTTTTGCTTGATGGATTCACATAATCAAATCCGTTTTCCTAAAGGAGTAGTAATTAAAGCCACCGCCAATAATACACCCAAATTCCATGTGAGTCCAACTTCTCCTCCTCCCAAACTCCCAACCAGAAGTTTGATCCAGAATTTGGAAAATGACCGTTTCAACTTTCCAATCAACACCAACAATACTAGCCATAGTTCTGATGATCCAACGGTGATTCCTGAGCTGTACGCAATCATGGATACCATTGCAGACAGAGTGGAGATGCACAAAAACATCGGAGCTCAGCGTGACAACTGGAACCATCTCCTTTTAATCTCCATCAACGCAATCACTCTCACTGCTGCAACCATGGCGGGACTAGCTGCAGCTGCTAGGGCTATTTCTACTGCAAAAGCACCCTTTATGGCTCTCAAGCTATCTTCCACTCTTCTCTACGTATCCGCCGCCGCCATGCTGGCGGTGACGAACAAAATCCAACCGTCCCAGCTCGCTGAGGAGCAGCGGAACGGGGCAAGGCTGTTCAAACAGCTCCATGCACAGATTCAAACAACCCTATCTCTCAACAATAACAGTGTGAGTAAAATTAATGTGAATGAAACAATAGAGAAAGTGTTGGCATTGGACAGGGCTTACCCTCTTCCTTTGTTAGGTGAAATGCTTGACAAGTTCCCGAAAACTGTGGAGCCCACAGTGTGGTGGCCAAGAGTACAGAATCATCAAATTAGGGTTCAGGGGGTTGAAGGTAATTTAGTACATGGAAATAATGGGTGGAGTGAGAAGTTGGAAGGGGAGATGAGAGAGGTTGCTAGGGCTTTGAAGAGCAAGGATGTGCCTGAGTACTTGAGATTGACTGAAATAGCCTTGAAAGTCAACAAAGTTTTGGCTATTTCTGGTCCTATTTTAACTGCCCTTGCAGCATTGGGGTCTGTTTTTGTGGGTTCAAGTAGCAATCTTGGGGTGTGGGGAGTAATGGTAGGGGTGGTTTGTGGAGCTTTGGCTAGTGTTGTGAATACATTGGAGCATGGGGGGCAAGTCGGGATGGTGGTTGAAATGTATAGGAGCAATGCTGGTTTTTTTAGGCTGATGCAAGAGAATATCGAATCAAATTTGAGAGAGAAACAAGTTGAGAGGAGAGAGAATGGGGGACTGTTTGAGTTGAAGGTGGCTTTGCAGCTTGGAAGGAGCTTATCAGAACTCAGTCATCTTGCTGTTTTCTCTGAAAAGTTTGCAAGCAAGCTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

424

Amino Acids

46.47

Weight (kDa)

9.31

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 84 - 408 4.7e-149 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 169, 720
AciI CCGC 7 cut(s) 90, 380, 468, 471, 474, 485, 529
AclI AACGTT 1 cut(s) 8
AclWI GGATC 2 cut(s) 154, 224
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 3 cut(s) 107, 166, 1132
AdeI CACNNNGTG 1 cut(s) 720
AfaI GTAC 4 cut(s) 254, 735, 780, 866
AfiI CCNNNNNNNGG 3 cut(s) 169, 674, 720
Alw21I GWGCWC 1 cut(s) 310
AlwI GGATC 2 cut(s) 154, 224
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 7 cut(s) 368, 389, 392, 395, 526, 941, 1198
ApoI RAATTY 3 cut(s) 107, 166, 1132
AseI ATTAAT 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 920
AsuHPI GGTGA 3 cut(s) 250, 499, 689
AvaII GGWCC 1 cut(s) 920
BalI TGGCCA 1 cut(s) 727
BanII GRGCYC 2 cut(s) 310, 714
Bbv12I GWGCWC 1 cut(s) 310
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 7 cut(s) 355, 376, 382, 404, 538, 953, 1210
BccI CCATC 4 cut(s) 31, 336, 353, 1060
BciVI GTATCC 2 cut(s) 259, 475
BfaI CTAG 6 cut(s) 213, 386, 399, 834, 1026, 1270
BfmI CTRYAG 1 cut(s) 390
BfuI GTATCC 2 cut(s) 259, 475
BglI GCCNNNNNGGC 1 cut(s) 482
BisI GCNGC 9 cut(s) 369, 390, 393, 396, 471, 474, 527, 942, 1199
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 9 cut(s) 370, 391, 394, 397, 472, 475, 528, 943, 1200
BmcAI AGTACT 1 cut(s) 866
Bme18I GGWCC 1 cut(s) 920
BmgT120I GGNCC 1 cut(s) 920
BmiI GGNNCC 2 cut(s) 326, 711
BmsI GCATC 2 cut(s) 279, 1101
Bpu10I CCTNAGC 2 cut(s) 246, 519
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 422, 889
BsaAI YACGTR 1 cut(s) 463
BsaJI CCNNGG 1 cut(s) 375
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 984, 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 674, 720
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 2 cut(s) 270, 1096
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 2 cut(s) 859, 1071
BseLI CCNNNNNNNGG 3 cut(s) 169, 674, 720
BseMI GCAATG 2 cut(s) 270, 1096
BseMII CTCAG 5 cut(s) 237, 323, 510, 852, 1237
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 4 cut(s) 120, 123, 536, 1171
BseXI GCAGC 7 cut(s) 355, 376, 382, 404, 538, 953, 1210
BseYI CCCAGC 1 cut(s) 510
BshFI GGCC 1 cut(s) 727
BsiHKAI GWGCWC 1 cut(s) 310
BslFI GGGAC 3 cut(s) 396, 493, 1185
BslI CCNNNNNNNGG 3 cut(s) 169, 674, 720
BsmFI GGGAC 3 cut(s) 396, 493, 1185
BsnI GGCC 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 310, 714
Bsp143I GATC 2 cut(s) 159, 229
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 7 cut(s) 90, 380, 468, 471, 474, 485, 529
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 5 cut(s) 238, 322, 511, 853, 1236
BspLI GGNNCC 2 cut(s) 326, 711
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 2 cut(s) 154, 224
BspQI GCTCTTC 1 cut(s) 839
BsrDI GCAATG 2 cut(s) 270, 1096
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 2 cut(s) 159, 229
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 7 cut(s) 182, 238, 507, 599, 706, 718, 1176
Bst6I CTCTTC 3 cut(s) 459, 669, 839
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 4 cut(s) 483, 516, 1258, 1262
BstDEI CTNAG 5 cut(s) 246, 309, 519, 861, 1223
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 672
BstF5I GGATG 2 cut(s) 859, 1071
BstKTI GATC 2 cut(s) 162, 232
BstMBI GATC 2 cut(s) 159, 229
BstMWI GCNNNNNNNGC 7 cut(s) 377, 386, 395, 401, 482, 941, 1054
BstSFI CTRYAG 1 cut(s) 390
BstSNI TACGTA 1 cut(s) 463
BstV1I GCAGC 7 cut(s) 355, 376, 382, 404, 538, 953, 1210
BsuI GTATCC 2 cut(s) 259, 475
BsuRI GGCC 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 859, 1071
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 363, 604, 723
Cac8I GCNNGC 4 cut(s) 483, 516, 1258, 1262
Cfr13I GGNCC 1 cut(s) 920
Csp6I GTAC 4 cut(s) 253, 734, 779, 865
CviAII CATG 7 cut(s) 113, 262, 376, 478, 560, 782, 1049
CviQI GTAC 4 cut(s) 253, 734, 779, 865
DdeI CTNAG 5 cut(s) 246, 309, 519, 861, 1223
DpnI GATC 2 cut(s) 161, 231
DpnII GATC 2 cut(s) 159, 229
DraIII CACNNNGTG 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 725
Eam1104I CTCTTC 3 cut(s) 459, 669, 839
EarI CTCTTC 3 cut(s) 459, 669, 839
EciI GGCGGA 1 cut(s) 457
Ecl136II GAGCTC 1 cut(s) 308
Eco105I TACGTA 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 310, 714
Eco47I GGWCC 1 cut(s) 920
Eco53kI GAGCTC 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 672
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 2 cut(s) 310, 714
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 7 cut(s) 116, 265, 379, 481, 563, 785, 1052
FaqI GGGAC 3 cut(s) 396, 493, 1185
FatI CATG 7 cut(s) 112, 261, 375, 477, 559, 781, 1048
FauI CCCGC 1 cut(s) 373
Fnu4HI GCNGC 9 cut(s) 369, 390, 393, 396, 471, 474, 527, 942, 1199
FokI GGATG 2 cut(s) 866, 1078
FriOI GRGCYC 2 cut(s) 310, 714
Fsp4HI GCNGC 9 cut(s) 369, 390, 393, 396, 471, 474, 527, 942, 1199
FspBI CTAG 6 cut(s) 213, 386, 399, 834, 1026, 1270
GluI GCNGC 9 cut(s) 369, 390, 393, 396, 471, 474, 527, 942, 1199
GsaI CCCAGC 1 cut(s) 514
HaeIII GGCC 1 cut(s) 727
Hin1II CATG 7 cut(s) 116, 265, 379, 481, 563, 785, 1052
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 7 cut(s) 24, 41, 118, 241, 568, 739, 1127
HphI GGTGA 3 cut(s) 250, 499, 689
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 4 cut(s) 226, 305, 1219, 1246
Hpy188III TCNNGA 5 cut(s) 21, 163, 245, 697, 1063
Hpy8I GTNNAC 1 cut(s) 898
HpyAV CCTTC 4 cut(s) 761, 806, 1180, 1200
HpyCH4III ACNGT 7 cut(s) 182, 238, 507, 599, 706, 718, 1176
HpyCH4IV ACGT 2 cut(s) 8, 462
HpyF10VI GCNNNNNNNGC 7 cut(s) 377, 386, 395, 401, 482, 941, 1054
HpyF3I CTNAG 5 cut(s) 246, 309, 519, 861, 1223
HpySE526I ACGT 2 cut(s) 8, 462
Hsp92II CATG 7 cut(s) 116, 265, 379, 481, 563, 785, 1052
Kzo9I GATC 2 cut(s) 159, 229
LguI GCTCTTC 1 cut(s) 839
LmnI GCTCC 8 cut(s) 305, 523, 561, 709, 1016, 1045, 1086, 1209
Lsp1109I GCAGC 7 cut(s) 355, 376, 382, 404, 538, 953, 1210
LweI GCATC 2 cut(s) 279, 1101
MaeI CTAG 6 cut(s) 213, 386, 399, 834, 1026, 1270
MaeII ACGT 2 cut(s) 8, 462
MaeIII GTNAC 2 cut(s) 314, 487
MalI GATC 2 cut(s) 161, 231
MboI GATC 2 cut(s) 159, 229
MboII GAAGA 4 cut(s) 438, 446, 656, 856
MhlI GDGCHC 2 cut(s) 310, 714
MlsI TGGCCA 1 cut(s) 727
MluCI AATT 7 cut(s) 78, 107, 166, 609, 747, 772, 1132
MluNI TGGCCA 1 cut(s) 727
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 4 cut(s) 146, 257, 526, 789
MnlI CCTC 6 cut(s) 141, 144, 514, 672, 820, 1149
Mox20I TGGCCA 1 cut(s) 727
MscI TGGCCA 1 cut(s) 727
MseI TTAA 4 cut(s) 81, 338, 612, 929
Msp20I TGGCCA 1 cut(s) 727
MspA1I CMGCKG 2 cut(s) 395, 529
MwoI GCNNNNNNNGC 7 cut(s) 377, 386, 395, 401, 482, 941, 1054
NcoI CCATGG 1 cut(s) 375
NdeII GATC 2 cut(s) 159, 229
NlaIII CATG 7 cut(s) 116, 265, 379, 481, 563, 785, 1052
NlaIV GGNNCC 2 cut(s) 326, 711
NmuCI GTSAC 2 cut(s) 314, 487
PciSI GCTCTTC 1 cut(s) 839
PfeI GAWTC 6 cut(s) 24, 41, 241, 568, 739, 1127
PflMI CCANNNNNTGG 2 cut(s) 169, 720
PkrI GCNGC 9 cut(s) 370, 391, 394, 397, 472, 475, 528, 943, 1200
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Ppu21I YACGTR 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 310
Psp1406I AACGTT 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 510
PspN4I GGNNCC 2 cut(s) 326, 711
PspPI GGNCC 1 cut(s) 920
PstI CTGCAG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 4 cut(s) 254, 735, 780, 866
RsaNI GTAC 4 cut(s) 253, 734, 779, 865
SacI GAGCTC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 839
SaqAI TTAA 4 cut(s) 81, 338, 612, 929
SatI GCNGC 9 cut(s) 369, 390, 393, 396, 471, 474, 527, 942, 1199
Sau3AI GATC 2 cut(s) 159, 229
Sau96I GGNCC 1 cut(s) 920
ScaI AGTACT 1 cut(s) 866
SchI GAGTC 1 cut(s) 127
SduI GDGCHC 2 cut(s) 310, 714
SfaNI GCATC 2 cut(s) 279, 1101
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 920
SmlI CTYRAG 2 cut(s) 437, 868
SmoI CTYRAG 2 cut(s) 437, 868
SnaBI TACGTA 1 cut(s) 463
Sse9I AATT 7 cut(s) 78, 107, 166, 609, 747, 772, 1132
SsiI CCGC 7 cut(s) 90, 380, 468, 471, 474, 485, 529
SspMI CTAG 6 cut(s) 213, 386, 399, 834, 1026, 1270
SstI GAGCTC 1 cut(s) 310
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 7 cut(s) 182, 238, 507, 599, 706, 718, 1176
TaiI ACGT 2 cut(s) 11, 465
TaqI TCGA 1 cut(s) 1125
TasI AATT 7 cut(s) 78, 107, 166, 609, 747, 772, 1132
TatI WGTACW 3 cut(s) 733, 778, 864
TauI GCSGC 2 cut(s) 473, 476
TfiI GAWTC 6 cut(s) 24, 41, 241, 568, 739, 1127
Tru1I TTAA 4 cut(s) 81, 338, 612, 929
Tru9I TTAA 4 cut(s) 81, 338, 612, 929
TscAI CASTG 3 cut(s) 370, 604, 723
TseFI GTSAC 2 cut(s) 314, 487
TseI GCWGC 7 cut(s) 368, 389, 392, 395, 526, 941, 1198
Tsp45I GTSAC 2 cut(s) 314, 487
TspDTI ATGAA 1 cut(s) 636
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 370, 604, 723
Van91I CCANNNNNTGG 2 cut(s) 169, 720
VpaK11BI GGWCC 1 cut(s) 920
VspI ATTAAT 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 672
XapI RAATTY 3 cut(s) 107, 166, 1132
XspI CTAG 6 cut(s) 213, 386, 399, 834, 1026, 1270
ZrmI AGTACT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.