Rroxscaffold_1G00073240

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014683
Physical Location & Seq
Forward (+)
94176989 .. 94178131
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073240.1

Sequence Viewer

Length: 1143 bp
ATGAAAACTGGTGCTTACAAAATTCCAAGCTCCACTGTGCAAAAGGAAATTGATTCTAATCCTACCATTCACAGTACTAATTCTGCATTACATAGTAGTGCAAAACTTTGTGATCCAGTGGTGATGTCTGAGATATATGCAATCATGGAGGTAGTTGCTGATAGAGTGGAGATGCACAAGAACATTGGAGCACAGCGCGACAATTGGAACCACCTCCTTCTGAGTTCCATCAATGTTATCACTCTCACTGCTGCAACCATTGCAGGAATTGCAGCTAGCAGTACTGCAAGTGCTGGAGTACCCTTTACGGCACTCAAGGTCTCTTCCGCTCTTCTCTATGTAGCAGCCACTGGAATGTTGGTGGTGATGAACATGATCCAGCCGTCCCAACTAGCCGAAGAACAGAGAATTGCAGCCAGATTGTTCAAGCAGCTTCATAGCCAGATTCAAACAACAGTGTCTCTTCAAAACCCGAATACAATGGACGTGAATAAAGCTATAGAGAAAGTGTTGGCATTGGACAGGGCATATCCACTTCCTTTATTAGGTGAAATGCTTGACAAATTTCCCAAAACTGTGGAGCCTGCAATATGGTGGCCGCAACAGAAGCAGCTTAGCCGGCGAGGAGTACCTGATCAAGGAATTGAAGGAAGATTGGCAAATAGATCCAACAGTTGGAGTGCAAAACTTGAAGAGGAAATGACAGAGATTGTTGGGGTATTGAAGAGCAAGGATGTGCCAGAGTACCTGAGGTTGACCGAGATAGCCTTGAAAATTAACAGAATTCTAGCCATTTCTGGCCCTCTTTTAACAGGGCTTGCAGCTTTGGGATCTGCTTTTTTGGGTTCTAGTACTTTTGGAGTTTGGAGCGGGATGGTGGGAGTCATTGGGGGAGCTTTGGCAAGTGTGGTGAACACAATAGAGCATGGGGGTCAAGTTGGAATGGTGGTTGAAATGTATAGAAGCAATGCTGGTTTCTTTAGGCTCATGCAAGAGACCATAGAAGCAAATTTAAGAGAAAAAGAAGTTGGGAGAAGGGAGAATGGGCAAGTGCTTGAAATGAAGGTGGCTTTGCAGCTAGGAAGGAGCCTATCAGAACTCAAGCATCTTTCTGTCTTTTCTTATGGAAGCAAGCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

380

Amino Acids

41.33

Weight (kDa)

8.81

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 45 - 368 7.4e-152 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 675
AccBSI CCGCTC 2 cut(s) 329, 870
AccII CGCG 1 cut(s) 198
AciI CCGC 3 cut(s) 327, 599, 870
AclWI GGATC 4 cut(s) 107, 370, 660, 838
AcoI YGGCCR 1 cut(s) 596
AcsI RAATTY 4 cut(s) 21, 563, 783, 1009
AfaI GTAC 6 cut(s) 76, 283, 300, 630, 746, 853
AfiI CCNNNNNNNGG 3 cut(s) 545, 638, 675
AgsI TTSAA 9 cut(s) 427, 449, 467, 647, 692, 724, 772, 953, 1058
AjiI CACGTC 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 1011, 1043
AluBI AGCT 9 cut(s) 30, 275, 433, 497, 613, 824, 896, 1078, 1135
AluI AGCT 9 cut(s) 30, 275, 433, 497, 613, 824, 896, 1078, 1135
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 3 cut(s) 325, 465, 989
AlwI GGATC 4 cut(s) 107, 370, 660, 838
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 2 cut(s) 596, 799
ApeKI GCWGC 8 cut(s) 251, 272, 344, 413, 430, 610, 821, 1075
ApoI RAATTY 4 cut(s) 21, 563, 783, 1009
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 800
AsuHPI GGTGA 4 cut(s) 133, 376, 560, 922
AsuNHI GCTAGC 1 cut(s) 275
AxyI CCTNAGG 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 8 cut(s) 238, 284, 356, 425, 442, 622, 833, 1087
BccI CCATC 2 cut(s) 236, 868
BceAI ACGGC 2 cut(s) 324, 367
BclI TGATCA 1 cut(s) 634
BcoDI GTCTC 3 cut(s) 325, 465, 989
BfaI CTAG 6 cut(s) 276, 392, 788, 849, 1079, 1141
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 9 cut(s) 252, 273, 345, 414, 431, 599, 611, 822, 1076
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 9 cut(s) 253, 274, 346, 415, 432, 600, 612, 823, 1077
BmcAI AGTACT 3 cut(s) 76, 283, 853
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 3 cut(s) 209, 582, 1088
BmsI GCATC 2 cut(s) 162, 1114
BmtI GCTAGC 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 614
BpuEI CTTGAG 2 cut(s) 299, 1085
BsaBI GATNNNNATC 1 cut(s) 57
BsaI GGTCTC 2 cut(s) 325, 989
Bsc4I CCNNNNNNNGG 3 cut(s) 545, 638, 675
Bse118I RCCGGY 1 cut(s) 618
Bse1I ACTGG 3 cut(s) 13, 116, 355
Bse21I CCTNAGG 1 cut(s) 749
Bse3DI GCAATG 2 cut(s) 258, 973
Bse8I GATNNNNATC 1 cut(s) 57
BseGI GGATG 2 cut(s) 739, 879
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 3 cut(s) 545, 638, 675
BseMI GCAATG 2 cut(s) 258, 973
BseMII CTCAG 3 cut(s) 120, 212, 740
BseNI ACTGG 3 cut(s) 13, 116, 355
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 8 cut(s) 238, 284, 356, 425, 442, 622, 833, 1087
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 598, 801
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 619
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 545, 638, 675
BsmAI GTCTC 3 cut(s) 325, 465, 989
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 2 cut(s) 598, 801
Bso31I GGTCTC 2 cut(s) 325, 989
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 5 cut(s) 112, 375, 634, 665, 830
Bsp1720I GCTNAGC 1 cut(s) 614
BspACI CCGC 3 cut(s) 327, 599, 870
BspANI GGCC 2 cut(s) 598, 801
BspCNI CTCAG 3 cut(s) 121, 213, 741
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 209, 582, 1088
BspOI GCTAGC 1 cut(s) 279
BspPI GGATC 4 cut(s) 107, 370, 660, 838
BspQI GCTCTTC 2 cut(s) 336, 719
BspTNI GGTCTC 2 cut(s) 325, 989
BsrBI CCGCTC 2 cut(s) 329, 870
BsrDI GCAATG 2 cut(s) 258, 973
BsrFI RCCGGY 1 cut(s) 618
BsrI ACTGG 3 cut(s) 13, 116, 355
BssAI RCCGGY 1 cut(s) 618
BssMI GATC 5 cut(s) 112, 375, 634, 665, 830
Bst4CI ACNGT 5 cut(s) 37, 74, 457, 577, 674
Bst6I CTCTTC 5 cut(s) 328, 336, 468, 687, 719
BstAPI GCANNNNNTGC 2 cut(s) 260, 269
BstC8I GCNNGC 5 cut(s) 277, 585, 620, 819, 1133
BstDEI CTNAG 4 cut(s) 129, 221, 614, 749
BstENI CCTNNNNNAGG 2 cut(s) 543, 636
BstF5I GGATG 2 cut(s) 739, 879
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 5 cut(s) 115, 378, 637, 668, 833
BstMAI GTCTC 3 cut(s) 325, 465, 989
BstMBI GATC 5 cut(s) 112, 375, 634, 665, 830
BstMWI GCNNNNNNNGC 4 cut(s) 260, 269, 607, 619
BstSFI CTRYAG 1 cut(s) 498
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 8 cut(s) 238, 284, 356, 425, 442, 622, 833, 1087
BstX2I RGATCY 2 cut(s) 665, 830
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 2 cut(s) 665, 830
Bsu36I CCTNAGG 1 cut(s) 749
BsuRI GGCC 2 cut(s) 598, 801
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 739, 879
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 5 cut(s) 33, 123, 246, 348, 462
Cac8I GCNNGC 5 cut(s) 277, 585, 620, 819, 1133
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 1 cut(s) 800
Csp6I GTAC 6 cut(s) 75, 282, 299, 629, 745, 852
CviAII CATG 4 cut(s) 145, 373, 926, 988
CviQI GTAC 6 cut(s) 75, 282, 299, 629, 745, 852
DdeI CTNAG 4 cut(s) 129, 221, 614, 749
DpnI GATC 5 cut(s) 114, 377, 636, 667, 832
DpnII GATC 5 cut(s) 112, 375, 634, 665, 830
EaeI YGGCCR 1 cut(s) 596
Eam1104I CTCTTC 5 cut(s) 328, 336, 468, 687, 719
EarI CTCTTC 5 cut(s) 328, 336, 468, 687, 719
Eco31I GGTCTC 2 cut(s) 325, 989
Eco81I CCTNAGG 1 cut(s) 749
EcoNI CCTNNNNNAGG 2 cut(s) 543, 636
EcoRI GAATTC 1 cut(s) 783
FaeI CATG 4 cut(s) 148, 376, 929, 991
FaqI GGGAC 1 cut(s) 370
FatI CATG 4 cut(s) 144, 372, 925, 987
FauI CCCGC 1 cut(s) 863
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 9 cut(s) 252, 273, 345, 414, 431, 599, 611, 822, 1076
FokI GGATG 2 cut(s) 746, 886
Fsp4HI GCNGC 9 cut(s) 252, 273, 345, 414, 431, 599, 611, 822, 1076
FspBI CTAG 6 cut(s) 276, 392, 788, 849, 1079, 1141
GlaI GCGC 1 cut(s) 197
GluI GCNGC 9 cut(s) 252, 273, 345, 414, 431, 599, 611, 822, 1076
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 2 cut(s) 598, 801
HapII CCGG 1 cut(s) 619
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 148, 376, 929, 991
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HinfI GANTC 3 cut(s) 53, 445, 882
HpaII CCGG 1 cut(s) 619
HphI GGTGA 4 cut(s) 133, 376, 560, 922
Hpy166II GTNNAC 2 cut(s) 756, 913
Hpy188I TCNGA 3 cut(s) 130, 222, 1096
Hpy8I GTNNAC 2 cut(s) 756, 913
HpyAV CCTTC 5 cut(s) 227, 641, 1029, 1057, 1077
HpyCH4III ACNGT 5 cut(s) 37, 74, 457, 577, 674
HpyCH4IV ACGT 1 cut(s) 486
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 269, 607, 619
HpyF3I CTNAG 4 cut(s) 129, 221, 614, 749
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 4 cut(s) 148, 376, 929, 991
HspAI GCGC 1 cut(s) 196
KroI GCCGGC 1 cut(s) 618
KroNI GCCGGC 1 cut(s) 620
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 5 cut(s) 112, 375, 634, 665, 830
LguI GCTCTTC 2 cut(s) 336, 719
LmnI GCTCC 6 cut(s) 35, 188, 580, 867, 893, 1086
Lsp1109I GCAGC 8 cut(s) 238, 284, 356, 425, 442, 622, 833, 1087
LweI GCATC 2 cut(s) 162, 1114
MaeI CTAG 6 cut(s) 276, 392, 788, 849, 1079, 1141
MaeII ACGT 1 cut(s) 486
MalI GATC 5 cut(s) 114, 377, 636, 667, 832
MbiI CCGCTC 2 cut(s) 329, 870
MboI GATC 5 cut(s) 112, 375, 634, 665, 830
MboII GAAGA 7 cut(s) 315, 323, 410, 455, 663, 704, 736
MfeI CAATTG 1 cut(s) 202
MflI RGATCY 2 cut(s) 665, 830
MhlI GDGCHC 1 cut(s) 193
MlyI GAGTC 1 cut(s) 891
MmeI TCCRAC 3 cut(s) 656, 693, 919
MnlI CCTC 6 cut(s) 142, 224, 617, 688, 744, 813
MroNI GCCGGC 1 cut(s) 618
MseI TTAA 3 cut(s) 777, 809, 1013
MslI CAYNNNNRTG 2 cut(s) 96, 353
MspI CCGG 1 cut(s) 619
MunI CAATTG 1 cut(s) 202
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 4 cut(s) 260, 269, 607, 619
NaeI GCCGGC 1 cut(s) 620
NdeII GATC 5 cut(s) 112, 375, 634, 665, 830
NgoMIV GCCGGC 1 cut(s) 618
NheI GCTAGC 1 cut(s) 275
NlaIII CATG 4 cut(s) 148, 376, 929, 991
NlaIV GGNNCC 3 cut(s) 209, 582, 1088
PciSI GCTCTTC 2 cut(s) 336, 719
PdiI GCCGGC 1 cut(s) 620
PfeI GAWTC 2 cut(s) 53, 445
PflMI CCANNNNNTGG 1 cut(s) 675
PkrI GCNGC 9 cut(s) 253, 274, 346, 415, 432, 600, 612, 823, 1077
PleI GAGTC 1 cut(s) 890
PpsI GAGTC 1 cut(s) 890
PspN4I GGNNCC 3 cut(s) 209, 582, 1088
PspPI GGNCC 1 cut(s) 800
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 2 cut(s) 665, 830
RsaI GTAC 6 cut(s) 76, 283, 300, 630, 746, 853
RsaNI GTAC 6 cut(s) 75, 282, 299, 629, 745, 852
RseI CAYNNNNRTG 2 cut(s) 96, 353
SapI GCTCTTC 2 cut(s) 336, 719
SaqAI TTAA 3 cut(s) 777, 809, 1013
SatI GCNGC 9 cut(s) 252, 273, 345, 414, 431, 599, 611, 822, 1076
Sau3AI GATC 5 cut(s) 112, 375, 634, 665, 830
Sau96I GGNCC 1 cut(s) 800
ScaI AGTACT 3 cut(s) 76, 283, 853
SchI GAGTC 1 cut(s) 891
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 2 cut(s) 162, 1114
SfcI CTRYAG 1 cut(s) 498
SmiMI CAYNNNNRTG 2 cut(s) 96, 353
SmlI CTYRAG 2 cut(s) 314, 1100
SmoI CTYRAG 2 cut(s) 314, 1100
SsiI CCGC 3 cut(s) 327, 599, 870
SspMI CTAG 6 cut(s) 276, 392, 788, 849, 1079, 1141
TaaI ACNGT 5 cut(s) 37, 74, 457, 577, 674
TaiI ACGT 1 cut(s) 489
TaqII GACCGA 1 cut(s) 773
TatI WGTACW 3 cut(s) 74, 281, 851
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 2 cut(s) 53, 445
Tru1I TTAA 3 cut(s) 777, 809, 1013
Tru9I TTAA 3 cut(s) 777, 809, 1013
TscAI CASTG 5 cut(s) 40, 123, 253, 355, 462
TseI GCWGC 8 cut(s) 251, 272, 344, 413, 430, 610, 821, 1075
TspDTI ATGAA 4 cut(s) 17, 383, 425, 1076
TspRI CASTG 5 cut(s) 40, 123, 253, 355, 462
Van91I CCANNNNNTGG 1 cut(s) 675
XagI CCTNNNNNAGG 2 cut(s) 543, 636
XapI RAATTY 4 cut(s) 21, 563, 783, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 6 cut(s) 276, 392, 788, 849, 1079, 1141
ZrmI AGTACT 3 cut(s) 76, 283, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.