MD10G1324000.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014683
Physical Location & Seq
Forward (+)
40477170 .. 40478450
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1324000.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGCAGCTTTTCAAGCTTCAAGCATCTTTTGCTTGATGGGATCACCTAATCAAATGCGTTTTCAAAAACGAGTAATCAAAGCTACTACCAATAATACTCCAAAATTCCAGGTGAGTCCTCCAACTTCTCCTCCTCCCAAGCTCCCAACCAAAAGTTTTATCCAGAACTTGGAAAACGATTATTTCCACTCTTCCATCAAAATCAACACCACTGGCAATATGTCAGATGATCCAACGGTGATTCCTAAGCTGTATGCAATCATGGAAGCCATTGCAGACAGAGTGGAGATGCATAAGAACATCGGAGCTCAGCGTGACAATTGGAACCATCTCCTTTTAATCTCCATCAATGCAATAACTCTCACTGCTGCAACCATGGCAGGACTTGCAGCTGCTGCTAGTACTATTTCTACTGCAAAAGCACCCTTTATGGCTCTCAAGCTCTCTTCCACTTTTCTCTACGTATCCGCCACCGCCATGCTGGCGGTGACAAACAAAATCCAACCGTCCCAGCTCGCCGAGGAGCAGCAGAACGCGGCGAGGCTGTTCAAGCAGCTCCATGCACAGATTCAAACAACCCTATCTCTCAACAACAACAGTGTAAGTAATAATATTAATAATGTGGATGTGAATGAAGCTATAGATAAAGTGTTGGCATTGGACAGGGCTTACCCTCTTCCTTTGTTAGGTGAAATGCTCGACAAGTTTCCCAAAACTGTGGAGCCCACAGTGTGGTGGCCAAGAGTACAGAATCATCAAAATAGGGTTCGGGGGGTTGAAGGTGATTTAGTACATGGGAATAATGGGTGGAATGAGAAGTTGGAAGAGGAGATGAGAGAGGTTGCTAGGGTTTTGAAGAGCAAGGATGTGCCTGAGTACTTGAGATTGACTGAAATAGCCTTGAAAGTCAACAGAGTTTTGGCTATTTCTGGTCCTATTTTAACTGCCCTTGCAGCTTTGGGGTCTAATTTTGTGGGTTCAAGTAGCAATCTTGGGGTGTGGGGAGTAATGGTAGGGGTCATATGTGGAGCTTTGGCTAGTGTTGTGAATACATTGCAGCATGGTGGGCAAGTTGGGATGGTGGTTGAAATGTACAGGAGCAATGCTGGTTTTTTTAGGCTGATGCAAGAGAGTATTGAATCAAATTTGAGAGAGAAACAAGTTGAGAGGAGAGAGAATGGGGGAGTGTTTGAATTGAAGGTGGCTTTGCAGCTTGGAAGGAGCTTATCAGAACTCAATCTTGCTGTTTTCCCTGAAAAGTTTGCAAGCAAGCTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

427

Amino Acids

46.86

Weight (kDa)

9.18

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 83 - 412 1.4e-150 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 169, 732
AccII CGCG 1 cut(s) 536
AciI CCGC 4 cut(s) 468, 474, 485, 536
AclWI GGATC 2 cut(s) 49, 224
AcoI YGGCCR 1 cut(s) 737
AcsI RAATTY 2 cut(s) 104, 1144
AdeI CACNNNGTG 1 cut(s) 732
AfaI GTAC 5 cut(s) 403, 747, 792, 878, 1094
AfiI CCNNNNNNNGG 3 cut(s) 169, 686, 732
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
Alw21I GWGCWC 1 cut(s) 310
AlwI GGATC 2 cut(s) 49, 224
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 1 cut(s) 737
ApoI RAATTY 2 cut(s) 104, 1144
AseI ATTAAT 1 cut(s) 615
AspS9I GGNCC 1 cut(s) 932
AsuHPI GGTGA 6 cut(s) 36, 124, 250, 499, 701, 794
AvaII GGWCC 1 cut(s) 932
BalI TGGCCA 1 cut(s) 739
BanII GRGCYC 2 cut(s) 310, 726
Bbv12I GWGCWC 1 cut(s) 310
BccI CCATC 5 cut(s) 31, 203, 336, 353, 1072
BciT130I CCWGG 1 cut(s) 110
BciVI GTATCC 1 cut(s) 475
BfaI CTAG 4 cut(s) 399, 846, 1038, 1279
BfmI CTRYAG 1 cut(s) 639
BfuI GTATCC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 482
BlpI GCTNAGC 1 cut(s) 309
BmcAI AGTACT 2 cut(s) 403, 878
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 932
BmgT120I GGNCC 1 cut(s) 932
BmiI GGNNCC 2 cut(s) 326, 723
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 3 cut(s) 33, 279, 1113
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 422, 901
BsaAI YACGTR 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 375, 519
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 996, 1026
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 686, 732
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 3 cut(s) 270, 1052, 1108
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 375, 519
BseGI GGATG 3 cut(s) 631, 871, 1083
BseLI CCNNNNNNNGG 3 cut(s) 169, 686, 732
BseMI GCAATG 3 cut(s) 270, 1052, 1108
BseMII CTCAG 2 cut(s) 323, 864
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 5 cut(s) 120, 123, 536, 842, 1183
BseYI CCCAGC 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 1 cut(s) 739
BsiHKAI GWGCWC 1 cut(s) 310
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 169, 686, 732
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 310, 726
Bsp1407I TGTACA 1 cut(s) 1092
Bsp143I GATC 2 cut(s) 41, 229
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 468, 474, 485, 536
BspANI GGCC 1 cut(s) 739
BspCNI CTCAG 2 cut(s) 322, 865
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 2 cut(s) 326, 723
BspPI GGATC 2 cut(s) 49, 224
BspQI GCTCTTC 1 cut(s) 851
BsrDI GCAATG 3 cut(s) 270, 1052, 1108
BsrGI TGTACA 1 cut(s) 1092
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 375, 519
BssMI GATC 2 cut(s) 41, 229
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 5 cut(s) 238, 507, 599, 718, 730
Bst6I CTCTTC 5 cut(s) 196, 451, 681, 819, 851
BstAPI GCANNNNNTGC 3 cut(s) 30, 386, 395
BstAUI TGTACA 1 cut(s) 1092
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 4 cut(s) 483, 516, 1267, 1271
BstDEI CTNAG 3 cut(s) 246, 309, 873
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 3 cut(s) 631, 871, 1083
BstFNI CGCG 1 cut(s) 536
BstKTI GATC 2 cut(s) 44, 232
BstMBI GATC 2 cut(s) 41, 229
BstMWI GCNNNNNNNGC 9 cut(s) 14, 30, 377, 386, 395, 482, 550, 953, 1066
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 639
BstSNI TACGTA 1 cut(s) 463
BstUI CGCG 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 718
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 1 cut(s) 739
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 3 cut(s) 631, 871, 1083
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 4 cut(s) 210, 363, 604, 735
Cac8I GCNNGC 4 cut(s) 483, 516, 1267, 1271
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 932
Csp6I GTAC 5 cut(s) 402, 746, 791, 877, 1093
CviAII CATG 6 cut(s) 262, 376, 478, 560, 794, 1061
CviQI GTAC 5 cut(s) 402, 746, 791, 877, 1093
DdeI CTNAG 3 cut(s) 246, 309, 873
DpnI GATC 2 cut(s) 43, 231
DpnII GATC 2 cut(s) 41, 229
DraIII CACNNNGTG 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 5 cut(s) 196, 451, 681, 819, 851
EarI CTCTTC 5 cut(s) 196, 451, 681, 819, 851
EciI GGCGGA 1 cut(s) 457
Ecl136II GAGCTC 1 cut(s) 308
Eco105I TACGTA 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 310, 726
Eco47I GGWCC 1 cut(s) 932
Eco53kI GAGCTC 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 310, 726
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 6 cut(s) 265, 379, 481, 563, 797, 1064
FaqI GGGAC 1 cut(s) 493
FatI CATG 6 cut(s) 261, 375, 477, 559, 793, 1060
FauNDI CATATG 1 cut(s) 1022
FokI GGATG 3 cut(s) 638, 878, 1090
FriOI GRGCYC 2 cut(s) 310, 726
FspBI CTAG 4 cut(s) 399, 846, 1038, 1279
GsaI CCCAGC 1 cut(s) 514
HaeIII GGCC 1 cut(s) 739
Hin1II CATG 6 cut(s) 265, 379, 481, 563, 797, 1064
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 5 cut(s) 115, 241, 568, 751, 1139
HphI GGTGA 6 cut(s) 36, 124, 250, 499, 701, 794
Hpy166II GTNNAC 1 cut(s) 910
Hpy188I TCNGA 3 cut(s) 226, 305, 1231
Hpy188III TCNNGA 1 cut(s) 163
Hpy8I GTNNAC 1 cut(s) 910
HpyAV CCTTC 3 cut(s) 773, 1192, 1212
HpyCH4III ACNGT 5 cut(s) 238, 507, 599, 718, 730
HpyCH4IV ACGT 1 cut(s) 462
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 30, 377, 386, 395, 482, 550, 953, 1066
HpyF3I CTNAG 3 cut(s) 246, 309, 873
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 6 cut(s) 265, 379, 481, 563, 797, 1064
Kzo9I GATC 2 cut(s) 41, 229
LguI GCTCTTC 1 cut(s) 851
LmnI GCTCC 8 cut(s) 147, 305, 523, 561, 721, 1028, 1098, 1221
LweI GCATC 3 cut(s) 33, 279, 1113
MaeI CTAG 4 cut(s) 399, 846, 1038, 1279
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 314, 487
MalI GATC 2 cut(s) 43, 231
MboI GATC 2 cut(s) 41, 229
MboII GAAGA 5 cut(s) 183, 438, 668, 836, 868
MfeI CAATTG 1 cut(s) 319
MhlI GDGCHC 2 cut(s) 310, 726
MlsI TGGCCA 1 cut(s) 739
MluCI AATT 5 cut(s) 104, 319, 967, 1144, 1193
MluNI TGGCCA 1 cut(s) 739
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 4 cut(s) 146, 257, 526, 801
MnlI CCTC 9 cut(s) 129, 141, 144, 514, 534, 684, 820, 832, 1161
Mox20I TGGCCA 1 cut(s) 739
Mph1103I ATGCAT 1 cut(s) 294
MscI TGGCCA 1 cut(s) 739
MseI TTAA 3 cut(s) 338, 615, 941
MslI CAYNNNNRTG 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 739
MspA1I CMGCKG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 9 cut(s) 14, 30, 377, 386, 395, 482, 550, 953, 1066
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 1022
NdeII GATC 2 cut(s) 41, 229
NlaIII CATG 6 cut(s) 265, 379, 481, 563, 797, 1064
NlaIV GGNNCC 2 cut(s) 326, 723
NmeAIII GCCGAG 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 314, 487
NsiI ATGCAT 1 cut(s) 294
PciSI GCTCTTC 1 cut(s) 851
PfeI GAWTC 4 cut(s) 241, 568, 751, 1139
PflMI CCANNNNNTGG 2 cut(s) 169, 732
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 108
PspFI CCCAGC 1 cut(s) 510
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 2 cut(s) 326, 723
PspPI GGNCC 1 cut(s) 932
PstNI CAGNNNCTG 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 5 cut(s) 403, 747, 792, 878, 1094
RsaNI GTAC 5 cut(s) 402, 746, 791, 877, 1093
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 3 cut(s) 338, 615, 941
Sau3AI GATC 2 cut(s) 41, 229
Sau96I GGNCC 1 cut(s) 932
ScaI AGTACT 2 cut(s) 403, 878
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 2 cut(s) 310, 726
SfaNI GCATC 3 cut(s) 33, 279, 1113
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 437, 880
SmoI CTYRAG 2 cut(s) 437, 880
SnaBI TACGTA 1 cut(s) 463
Sse9I AATT 5 cut(s) 104, 319, 967, 1144, 1193
SsiI CCGC 4 cut(s) 468, 474, 485, 536
SspI AATATT 1 cut(s) 613
SspMI CTAG 4 cut(s) 399, 846, 1038, 1279
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 5 cut(s) 238, 507, 599, 718, 730
TaiI ACGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 699
TasI AATT 5 cut(s) 104, 319, 967, 1144, 1193
TatI WGTACW 5 cut(s) 401, 745, 790, 876, 1092
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 4 cut(s) 241, 568, 751, 1139
Tru1I TTAA 3 cut(s) 338, 615, 941
Tru9I TTAA 3 cut(s) 338, 615, 941
TscAI CASTG 4 cut(s) 217, 370, 604, 735
TseFI GTSAC 2 cut(s) 314, 487
Tsp45I GTSAC 2 cut(s) 314, 487
TspDTI ATGAA 1 cut(s) 648
TspRI CASTG 4 cut(s) 217, 370, 604, 735
Van91I CCANNNNNTGG 2 cut(s) 169, 732
VpaK11BI GGWCC 1 cut(s) 932
VspI ATTAAT 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 2 cut(s) 104, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XspI CTAG 4 cut(s) 399, 846, 1038, 1279
ZrmI AGTACT 2 cut(s) 403, 878
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.