RchiOBHm_Chr5g0002611

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014683
Physical Location & Seq
Reverse (-)
1558650 .. 1559282
633 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28397

Sequence Viewer

Length: 474 bp
ATGCTTGATAAATTTCCCAAAACTGTGGAGCCTGCAATATGGTGGCCGCAACAGAAGCAGCTTAGCCGACGAGGATACCTGAGGTTGACCGAGGTAGCCTTGAAAATTAACAGAATTCTAGCCATTTCTGGCCCTCTTTTAACAGGGCTTGCAGCTTTGGGATCTGCTTTTTTGGGTTCTAGTATTTTTGGAGTTTGGAGCGGGATGGTAGGAGTCATTGGGGGAGCTTTGGCAAGTGTGGTGAACACAATAGAGCATGGGGGTCAAGTTGGAATGGTGGTTGAAATGTATAGAAGCAATGCTGGTTTCTTTAGGCTCATACAAGAGACCATAGAAGCAAATTTAAGAGAAAAAGAAGTTGGGAGAAGGGAGAATGGGCAAGTGCTTGAAATGAAGGTGGCTTTGCATCTGGGAAGGAGCCTATCAGAACTCAAGCATCTTTCTGTCTTTTCTTATGGAAGCAAGCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

157

Amino Acids

17.24

Weight (kDa)

9.91

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 24 - 145 4.1e-54 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 201
AciI CCGC 2 cut(s) 47, 201
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 44
AcsI RAATTY 3 cut(s) 11, 114, 340
AgsI TTSAA 3 cut(s) 103, 284, 389
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 4 cut(s) 61, 155, 227, 466
AluI AGCT 4 cut(s) 61, 155, 227, 466
Alw26I GTCTC 1 cut(s) 320
AlwI GGATC 1 cut(s) 169
AoxI GGCC 2 cut(s) 44, 130
ApeKI GCWGC 2 cut(s) 58, 152
ApoI RAATTY 3 cut(s) 11, 114, 340
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 253
AxyI CCTNAGG 1 cut(s) 80
BbvI GCAGC 2 cut(s) 70, 164
BccI CCATC 1 cut(s) 199
BciVI GTATCC 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 3 cut(s) 119, 180, 472
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 3 cut(s) 47, 59, 153
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 3 cut(s) 48, 60, 154
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 30, 419
BmsI GCATC 2 cut(s) 415, 445
Bpu1102I GCTNAGC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 90
Bse21I CCTNAGG 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 210
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 70, 164
BshFI GGCC 2 cut(s) 46, 132
BsmAI GTCTC 1 cut(s) 320
BsnI GGCC 2 cut(s) 46, 132
Bso31I GGTCTC 1 cut(s) 320
Bsp143I GATC 1 cut(s) 161
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 2 cut(s) 47, 201
BspANI GGCC 2 cut(s) 46, 132
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 30, 419
BspPI GGATC 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 33, 150, 464
BstDEI CTNAG 2 cut(s) 62, 80
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 1 cut(s) 164
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 1 cut(s) 161
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 70, 164
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 161
Bsu36I CCTNAGG 1 cut(s) 80
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 2 cut(s) 46, 132
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 33, 150, 464
Cfr13I GGNCC 1 cut(s) 131
CviAII CATG 1 cut(s) 257
DdeI CTNAG 2 cut(s) 62, 80
DpnI GATC 1 cut(s) 163
DpnII GATC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 320
Eco81I CCTNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 114
FaeI CATG 1 cut(s) 260
FaiI YATR 6 cut(s) 40, 258, 291, 320, 332, 456
FatI CATG 1 cut(s) 256
FauI CCCGC 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 47, 59, 153
FokI GGATG 1 cut(s) 217
Fsp4HI GCNGC 3 cut(s) 47, 59, 153
FspBI CTAG 3 cut(s) 119, 180, 472
GluI GCNGC 3 cut(s) 47, 59, 153
HaeIII GGCC 2 cut(s) 46, 132
Hin1II CATG 1 cut(s) 260
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 1 cut(s) 213
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 87, 244
Hpy188I TCNGA 1 cut(s) 427
Hpy8I GTNNAC 2 cut(s) 87, 244
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 3 cut(s) 360, 388, 408
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 3 cut(s) 35, 152, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 2 cut(s) 62, 80
Hsp92II CATG 1 cut(s) 260
Kzo9I GATC 1 cut(s) 161
LmnI GCTCC 4 cut(s) 28, 198, 224, 417
LpnPI CCDG 6 cut(s) 45, 92, 114, 129, 288, 395
Lsp1109I GCAGC 2 cut(s) 70, 164
LweI GCATC 2 cut(s) 415, 445
MaeI CTAG 3 cut(s) 119, 180, 472
MalI GATC 1 cut(s) 163
MbiI CCGCTC 1 cut(s) 201
MboI GATC 1 cut(s) 161
MflI RGATCY 1 cut(s) 161
MluCI AATT 4 cut(s) 11, 105, 114, 340
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 4 cut(s) 65, 75, 85, 144
MseI TTAA 3 cut(s) 108, 140, 344
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 1 cut(s) 161
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 2 cut(s) 30, 419
PkrI GCNGC 3 cut(s) 48, 60, 154
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 30, 419
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 161
SaqAI TTAA 3 cut(s) 108, 140, 344
SatI GCNGC 3 cut(s) 47, 59, 153
Sau3AI GATC 1 cut(s) 161
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 222
SetI ASST 8 cut(s) 63, 81, 86, 96, 157, 229, 399, 468
SfaNI GCATC 2 cut(s) 415, 445
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 4 cut(s) 11, 105, 114, 340
SsiI CCGC 2 cut(s) 47, 201
SspMI CTAG 3 cut(s) 119, 180, 472
TaaI ACNGT 1 cut(s) 25
TaqII GACCGA 1 cut(s) 104
TasI AATT 4 cut(s) 11, 105, 114, 340
TauI GCSGC 1 cut(s) 49
Tru1I TTAA 3 cut(s) 108, 140, 344
Tru9I TTAA 3 cut(s) 108, 140, 344
TseI GCWGC 2 cut(s) 58, 152
TspDTI ATGAA 1 cut(s) 407
XapI RAATTY 3 cut(s) 11, 114, 340
XspI CTAG 3 cut(s) 119, 180, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.