RchiOBHm_Chr5g0002621

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014683
Physical Location & Seq
Reverse (-)
1559284 .. 1559974
691 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28398

Sequence Viewer

Length: 651 bp
ATGGCAACCTTTCAAGTCTCAACCATATTTCCTTTGAGGGATTCTTCAAATCAAAAGCGTATTCCTAAAGGAGTAGTTATTGTAGCAACCAATAATGCACCAAATGGCAAGGTGAGGCCACTTACTTCTCTTCCCAAGCTCCCAACCAGAAGTTTGGTTGAGGGCTTGGACATGAAAACTGGTGCTTACAAAATTCCAAGCTCCACTGTGCAAAAGGAAATTGATTCTAATCCTACCATTCATAGTACTAATTCTGCATTACATAGTAGTGCAAAACTTTGTGATCCAGTGGTGATGTCTGAGATATATGCAATCATGGAGGTAGTTGCTGATAGAGTGGAGATGCACAAGAACATTGGAGCACAGCGCGACAATTGGAACCACCTCCTTCTGAGTTCCATCAATGTGATCACTCTCACTGCTGCAATCATTGCAGGAATTGCAGCTAGCAGTACTACAAGTGCTGGAGTACCCTTTACGGCACTCAAGGTCTCTTCCGCTCTTCTCTATGTAGCAGCCACTGGAATGTTGGTGGTGATGAACATGATCCAGCCGTCCCAACTAGCCGAAGAACAGAGAATTGCTGCCAGATTGTTCAAGCAGCTTCATAGCCAGATTCAAACAACAGTGTCACTTCAAAACCCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

216

Amino Acids

23.24

Weight (kDa)

9.63

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 102 - 215 1.8e-50 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 500
AccII CGCG 1 cut(s) 369
AciI CCGC 1 cut(s) 498
AclWI GGATC 2 cut(s) 278, 541
AcsI RAATTY 1 cut(s) 192
AfaI GTAC 3 cut(s) 247, 454, 471
AgsI TTSAA 5 cut(s) 14, 48, 598, 620, 638
AluBI AGCT 4 cut(s) 139, 201, 446, 604
AluI AGCT 4 cut(s) 139, 201, 446, 604
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 2 cut(s) 22, 496
AlwI GGATC 2 cut(s) 278, 541
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 5 cut(s) 422, 443, 515, 584, 601
ApoI RAATTY 1 cut(s) 192
AspLEI GCGC 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 124, 304, 547
AsuNHI GCTAGC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 5 cut(s) 409, 455, 527, 571, 613
BccI CCATC 1 cut(s) 407
BceAI ACGGC 2 cut(s) 495, 538
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 2 cut(s) 22, 496
BfaI CTAG 2 cut(s) 447, 563
BisI GCNGC 5 cut(s) 423, 444, 516, 585, 602
BlsI GCNGC 5 cut(s) 424, 445, 517, 586, 603
BmcAI AGTACT 2 cut(s) 247, 454
BmiI GGNNCC 1 cut(s) 380
BmsI GCATC 1 cut(s) 333
BmtI GCTAGC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 496
Bse1I ACTGG 3 cut(s) 184, 287, 526
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 228
BseJI GATNNNNATC 1 cut(s) 228
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 291, 383
BseNI ACTGG 3 cut(s) 184, 287, 526
BseXI GCAGC 5 cut(s) 409, 455, 527, 571, 613
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 364
BslFI GGGAC 1 cut(s) 541
BsmAI GTCTC 2 cut(s) 22, 496
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 283, 408, 546
BspACI CCGC 1 cut(s) 498
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 2 cut(s) 292, 384
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 380
BspOI GCTAGC 1 cut(s) 450
BspPI GGATC 2 cut(s) 278, 541
BspQI GCTCTTC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 496
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 3 cut(s) 184, 287, 526
BssMI GATC 3 cut(s) 283, 408, 546
Bst4CI ACNGT 2 cut(s) 208, 628
Bst6I CTCTTC 3 cut(s) 135, 499, 507
BstAPI GCANNNNNTGC 2 cut(s) 431, 440
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 2 cut(s) 300, 392
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 3 cut(s) 286, 411, 549
BstMAI GTCTC 2 cut(s) 22, 496
BstMBI GATC 3 cut(s) 283, 408, 546
BstMWI GCNNNNNNNGC 2 cut(s) 431, 440
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 5 cut(s) 409, 455, 527, 571, 613
BstXI CCANNNNNNTGG 1 cut(s) 154
BsuRI GGCC 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 5 cut(s) 204, 294, 417, 519, 633
Cac8I GCNNGC 1 cut(s) 448
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 369
Csp6I GTAC 3 cut(s) 246, 453, 470
CviAII CATG 3 cut(s) 172, 316, 544
CviQI GTAC 3 cut(s) 246, 453, 470
DdeI CTNAG 2 cut(s) 300, 392
DpnI GATC 3 cut(s) 285, 410, 548
DpnII GATC 3 cut(s) 283, 408, 546
Eam1104I CTCTTC 3 cut(s) 135, 499, 507
EarI CTCTTC 3 cut(s) 135, 499, 507
Eco31I GGTCTC 1 cut(s) 496
FaeI CATG 3 cut(s) 175, 319, 547
FaqI GGGAC 1 cut(s) 541
FatI CATG 3 cut(s) 171, 315, 543
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 5 cut(s) 423, 444, 516, 585, 602
Fsp4HI GCNGC 5 cut(s) 423, 444, 516, 585, 602
FspBI CTAG 2 cut(s) 447, 563
GlaI GCGC 1 cut(s) 368
GluI GCNGC 5 cut(s) 423, 444, 516, 585, 602
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 1 cut(s) 118
HhaI GCGC 1 cut(s) 369
Hin1II CATG 3 cut(s) 175, 319, 547
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 3 cut(s) 41, 224, 616
HphI GGTGA 3 cut(s) 124, 304, 547
Hpy188I TCNGA 2 cut(s) 301, 393
HpyAV CCTTC 1 cut(s) 398
HpyCH4III ACNGT 2 cut(s) 208, 628
HpyCH4V TGCA 9 cut(s) 98, 211, 257, 272, 311, 346, 425, 434, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 440
HpyF3I CTNAG 2 cut(s) 300, 392
Hsp92II CATG 3 cut(s) 175, 319, 547
HspAI GCGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 3 cut(s) 283, 408, 546
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 3 cut(s) 144, 206, 359
LpnPI CCDG 9 cut(s) 160, 165, 300, 420, 450, 507, 563, 601, 626
Lsp1109I GCAGC 5 cut(s) 409, 455, 527, 571, 613
LweI GCATC 1 cut(s) 333
MaeI CTAG 2 cut(s) 447, 563
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 285, 410, 548
MbiI CCGCTC 1 cut(s) 500
MboI GATC 3 cut(s) 283, 408, 546
MboII GAAGA 5 cut(s) 36, 122, 486, 494, 581
MfeI CAATTG 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 7 cut(s) 192, 219, 250, 373, 438, 579, 646
MnlI CCTC 5 cut(s) 30, 108, 154, 313, 395
MslI CAYNNNNRTG 3 cut(s) 267, 404, 524
MunI CAATTG 1 cut(s) 373
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 431, 440
NdeII GATC 3 cut(s) 283, 408, 546
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 3 cut(s) 175, 319, 547
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 630
PciSI GCTCTTC 1 cut(s) 507
PfeI GAWTC 3 cut(s) 41, 224, 616
PkrI GCNGC 5 cut(s) 424, 445, 517, 586, 603
PspN4I GGNNCC 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 521
RsaI GTAC 3 cut(s) 247, 454, 471
RsaNI GTAC 3 cut(s) 246, 453, 470
RseI CAYNNNNRTG 3 cut(s) 267, 404, 524
SapI GCTCTTC 1 cut(s) 507
SatI GCNGC 5 cut(s) 423, 444, 516, 585, 602
Sau3AI GATC 3 cut(s) 283, 408, 546
ScaI AGTACT 2 cut(s) 247, 454
SduI GDGCHC 1 cut(s) 364
SetI ASST 9 cut(s) 11, 114, 141, 203, 324, 387, 448, 492, 606
SfaNI GCATC 1 cut(s) 333
SmiMI CAYNNNNRTG 3 cut(s) 267, 404, 524
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 7 cut(s) 192, 219, 250, 373, 438, 579, 646
SsiI CCGC 1 cut(s) 498
SspMI CTAG 2 cut(s) 447, 563
TaaI ACNGT 2 cut(s) 208, 628
TasI AATT 7 cut(s) 192, 219, 250, 373, 438, 579, 646
TatI WGTACW 2 cut(s) 245, 452
TfiI GAWTC 3 cut(s) 41, 224, 616
TscAI CASTG 5 cut(s) 211, 294, 424, 526, 633
TseFI GTSAC 1 cut(s) 630
TseI GCWGC 5 cut(s) 422, 443, 515, 584, 601
Tsp45I GTSAC 1 cut(s) 630
TspDTI ATGAA 4 cut(s) 188, 230, 554, 596
TspRI CASTG 5 cut(s) 211, 294, 424, 526, 633
XapI RAATTY 1 cut(s) 192
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XspI CTAG 2 cut(s) 447, 563
ZrmI AGTACT 2 cut(s) 247, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.