Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013988

Physical Location & Seq

Genomic Coordinates Forward (+)

47071356 .. 47073240

Gene Structure 1885 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1354000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGGCAACTTTCCCAGTTGTTGACATGGACCTTATCAATGGTGAAGAGAGAGCAGCAACCTTGGAGAAGATCAATGATGCATGTGAGAACTGGGGTTTCTTTGAGTTGGTGAACCATGGGATATCAACTGAGCTTTTGGACACTGTGGAGAAGATGAACAAGGATCACTACAAGAAGACCATGGAGCAAAGGTTCAAGGAAATGGTGGCAGCCAAAGGCCTCGAAGCTGTCCAGTCCGAAATCCACGACTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTTCCTTCCTCAAACATTTCCGAAATCCCTGATCTCGAGGAAGATTACAGGAAGACCATGAAGGAATTTGCAGTGGAATTGGAGAAGCTAGCTGAGAAGCTTTTGGACTTGCTGTGTGAGAATCTTGGACTTGAGAAGGGCTATCTGAAGAAGGCTTTCTATGGATCCAAGGGTCCGAATTTTGGGACCAAGGTCAGCAACTACCCTCCATGTCCCAAGCCAGACCTGATCAAGGGACTCCGGGCCCACACCGACGCTGGTGGTATCATCCTGCTTTTCCAGGATGACAAGGTCAGCGGCCTCCAGCTCCTCAAGGATGGTGAATGGATGGATGTCCCCCCAGTGCACCACTCCATTGTCATCAACTTAGGTGACCAGATTGAGGTGATCACCAATGGGAAGTACAAGAGTATAATGCACCGGGTGATAGCTCAGTCGGACGGAACCAGAATGTCGATAGCCTCGTTCTACAACCCGGGCGACGATGCGTTTATCAGCCCGGCACCGGCATTGCTGGAGAAGAAATCTGAGGAAACCCCAACTTATCCCAAATTTTTGTTTGATGATTACATGAAGCTGTATTCTGGCCTCAAATTCCAAGCCAAGGAGCCAAGATTTGAAGCTATGAAGGCCAGGGAAACCACCCCTGTTGAAACTGCCCGAGGTCTGAGAGCCGTCCGATGGAACACGACAAAAAGAAATCAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0008152 GO:0009058 GO:0009692 GO:0009693 GO:0009987 GO:0010817 GO:0042445 GO:0042446 GO:0043449 GO:0043450 GO:0044237 GO:0044249 GO:0065007 GO:0065008 GO:0071704 GO:1900673 GO:1900674 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko01110 map00270 map01100 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

331

Amino Acids

37.52

Weight (kDa)

5.52

Isoelectric Point (pI)

41.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	5 - 97	6.5e-16	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	159 - 253	1.4e-32	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12010 AT1G62380
fragaria_vesca	FvH4_3g01280
malus_domestica	MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica	Prupe.4G013800_v2.0.a1
pyrus_communis	pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis	RchiOBHm_Chr5g0001831
rosa_laevigata	RLG00000030975
rosa_multiflora	Rmu_ssc0000237.1_g000010
rosa_roxburghii	Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa	Rorug04G0393500
rosa_samantha	Rh5DG016100
rosa_wichuraiana	Rw5G001410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	784
AccB7I	CCANNNNNTGG	1 cut(s)	250
AciI	CCGC	1 cut(s)	579
AclWI	GGATC	3 cut(s)	171, 441, 454
AcsI	RAATTY	4 cut(s)	347, 460, 833, 875
AcuI	CTGAAG	1 cut(s)	449
AdeI	CACNNNGTG	1 cut(s)	706
AfaI	GTAC	1 cut(s)	686
AfiI	CCNNNNNNNGG	7 cut(s)	250, 464, 514, 664, 787, 886, 963
AgsI	TTSAA	3 cut(s)	196, 902, 935
AjnI	CCWGG	2 cut(s)	561, 914
AloI	GAACNNNNNNTCC	2 cut(s)	176, 208
AluBI	AGCT	9 cut(s)	133, 227, 370, 374, 382, 589, 713, 859, 905
AluI	AGCT	9 cut(s)	133, 227, 370, 374, 382, 589, 713, 859, 905
Alw21I	GWGCWC	1 cut(s)	630
Alw44I	GTGCAC	1 cut(s)	626
AlwI	GGATC	3 cut(s)	171, 441, 454
Ama87I	CYCGRG	3 cut(s)	317, 757, 942
AoxI	GGCC	5 cut(s)	217, 525, 580, 868, 912
ApaI	GGGCCC	1 cut(s)	529
ApaLI	GTGCAC	1 cut(s)	626
ApeKI	GCWGC	2 cut(s)	53, 209
ApoI	RAATTY	4 cut(s)	347, 460, 833, 875
Asp700I	GAANNNNTTC	1 cut(s)	437
AspLEI	GCGC	1 cut(s)	279
AspS9I	GGNCC	5 cut(s)	28, 455, 468, 525, 526
AsuC2I	CCSGG	5 cut(s)	524, 704, 758, 759, 782
AsuHPI	GGTGA	7 cut(s)	53, 121, 614, 664, 665, 679, 718
AsuNHI	GCTAGC	1 cut(s)	370
AvaI	CYCGRG	3 cut(s)	317, 757, 942
AvaII	GGWCC	3 cut(s)	28, 455, 468
BaeGI	GKGCMC	2 cut(s)	529, 630
BamHI	GGATCC	1 cut(s)	446
BanI	GGYRCC	1 cut(s)	784
BanII	GRGCYC	1 cut(s)	529
BbsI	GAAGAC	2 cut(s)	182, 341
Bbv12I	GWGCWC	1 cut(s)	630
BbvI	GCAGC	2 cut(s)	65, 221
BccI	CCATC	3 cut(s)	593, 604, 957
BceAI	ACGGC	1 cut(s)	941
BciT130I	CCWGG	2 cut(s)	563, 916
BclI	TGATCA	2 cut(s)	510, 669
BcnI	CCSGG	5 cut(s)	524, 704, 758, 759, 782
BfaI	CTAG	1 cut(s)	371
BisI	GCNGC	3 cut(s)	54, 210, 580
BlsI	GCNGC	3 cut(s)	55, 211, 581
Bme1390I	CCNGG	7 cut(s)	524, 563, 704, 758, 759, 782, 916
Bme18I	GGWCC	3 cut(s)	28, 455, 468
BmeT110I	CYCGRG	3 cut(s)	317, 757, 942
BmgT120I	GGNCC	5 cut(s)	28, 455, 468, 525, 526
BmiI	GGNNCC	7 cut(s)	448, 456, 469, 527, 727, 786, 891
BmrFI	CCNGG	7 cut(s)	524, 563, 704, 758, 759, 782, 916
BmrI	ACTGGG	4 cut(s)	8, 100, 265, 617
BmsI	GCATC	2 cut(s)	67, 757
BmtI	GCTAGC	1 cut(s)	374
BmuI	ACTGGG	4 cut(s)	8, 100, 265, 617
BoxI	GACNNNNGTC	1 cut(s)	473
BpiI	GAAGAC	2 cut(s)	182, 341
BpmI	CTGGAG	2 cut(s)	569, 818
BpuEI	CTTGAG	2 cut(s)	434, 578
BpuMI	CCSGG	5 cut(s)	524, 704, 758, 759, 782
BsaJI	CCNNGG	9 cut(s)	60, 115, 180, 450, 471, 757, 885, 915, 943
BsaXI	ACNNNNNCTCC	2 cut(s)	176, 206
Bsc4I	CCNNNNNNNGG	7 cut(s)	250, 464, 514, 664, 787, 886, 963
Bse118I	RCCGGY	1 cut(s)	787
Bse1I	ACTGG	5 cut(s)	14, 95, 232, 260, 623
Bse3DI	GCAATG	1 cut(s)	791
BseBI	CCWGG	2 cut(s)	563, 916
BseDI	CCNNGG	9 cut(s)	60, 115, 180, 450, 471, 757, 885, 915, 943
BseGI	GGATG	5 cut(s)	549, 571, 604, 615, 619
BseLI	CCNNNNNNNGG	7 cut(s)	250, 464, 514, 664, 787, 886, 963
BseMI	GCAATG	1 cut(s)	791
BseMII	CTCAG	5 cut(s)	120, 366, 728, 801, 941
BseNI	ACTGG	5 cut(s)	14, 95, 232, 260, 623
BseRI	GAGGAG	1 cut(s)	581
BseSI	GKGCMC	2 cut(s)	529, 630
BseXI	GCAGC	2 cut(s)	65, 221
BshFI	GGCC	5 cut(s)	219, 527, 582, 870, 914
BshNI	GGYRCC	1 cut(s)	784
BsiHKAI	GWGCWC	1 cut(s)	630
BsiHKCI	CYCGRG	3 cut(s)	317, 757, 942
BsiSI	CCGG	5 cut(s)	523, 703, 758, 782, 788
BslFI	GGGAC	4 cut(s)	480, 481, 531, 602
BslI	CCNNNNNNNGG	7 cut(s)	250, 464, 514, 664, 787, 886, 963
BsmFI	GGGAC	4 cut(s)	480, 481, 531, 602
BsnI	GGCC	5 cut(s)	219, 527, 582, 870, 914
BsoBI	CYCGRG	3 cut(s)	317, 757, 942
Bsp120I	GGGCCC	1 cut(s)	525
Bsp1286I	GDGCHC	2 cut(s)	529, 630
Bsp143I	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
Bsp19I	CCATGG	2 cut(s)	115, 180
BspACI	CCGC	1 cut(s)	579
BspANI	GGCC	5 cut(s)	219, 527, 582, 870, 914
BspCNI	CTCAG	5 cut(s)	121, 367, 727, 802, 942
BspLI	GGNNCC	7 cut(s)	448, 456, 469, 527, 727, 786, 891
BspOI	GCTAGC	1 cut(s)	374
BspPI	GGATC	3 cut(s)	171, 441, 454
BspT107I	GGYRCC	1 cut(s)	784
BsrDI	GCAATG	1 cut(s)	791
BsrFI	RCCGGY	1 cut(s)	787
BsrI	ACTGG	5 cut(s)	14, 95, 232, 260, 623
BssAI	RCCGGY	1 cut(s)	787
BssECI	CCNNGG	9 cut(s)	60, 115, 180, 450, 471, 757, 885, 915, 943
BssMI	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
BssT1I	CCWWGG	6 cut(s)	60, 115, 180, 450, 471, 885
Bst2UI	CCWGG	2 cut(s)	563, 916
Bst4CI	ACNGT	1 cut(s)	145
Bst6I	CTCTTC	1 cut(s)	39
BstC8I	GCNNGC	1 cut(s)	372
BstDEI	CTNAG	6 cut(s)	129, 375, 649, 714, 810, 950
BstDSI	CCRYGG	2 cut(s)	115, 180
BstEII	GGTNACC	1 cut(s)	653
BstENI	CCTNNNNNAGG	1 cut(s)	512
BstF5I	GGATG	5 cut(s)	549, 571, 604, 615, 619
BstHHI	GCGC	1 cut(s)	279
BstKTI	GATC	6 cut(s)	72, 166, 316, 449, 513, 672
BstMBI	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
BstMWI	GCNNNNNNNGC	1 cut(s)	911
BstNI	CCWGG	2 cut(s)	563, 916
BstNSI	RCATGY	1 cut(s)	84
BstPAI	GACNNNNGTC	1 cut(s)	473
BstPI	GGTNACC	1 cut(s)	653
BstSCI	CCNGG	7 cut(s)	522, 561, 702, 756, 757, 780, 914
BstSLI	GKGCMC	2 cut(s)	529, 630
BstV1I	GCAGC	2 cut(s)	65, 221
BstV2I	GAAGAC	2 cut(s)	182, 341
BstX2I	RGATCY	1 cut(s)	446
BstYI	RGATCY	1 cut(s)	446
BsuRI	GGCC	5 cut(s)	219, 527, 582, 870, 914
BtgI	CCRYGG	2 cut(s)	115, 180
BtsCI	GGATG	5 cut(s)	549, 571, 604, 615, 619
BtsI	GCAGTG	1 cut(s)	360
BtsIMutI	CAGTG	3 cut(s)	141, 360, 630
Cac8I	GCNNGC	1 cut(s)	372
CfoI	GCGC	1 cut(s)	279
Cfr10I	RCCGGY	1 cut(s)	787
Cfr13I	GGNCC	5 cut(s)	28, 455, 468, 525, 526
Cfr9I	CCCGGG	1 cut(s)	757
CseI	GACGC	1 cut(s)	545
Csp6I	GTAC	1 cut(s)	685
CspCI	CAANNNNNGTGG	2 cut(s)	913, 948
CviAII	CATG	7 cut(s)	25, 81, 116, 181, 340, 492, 853
CviQI	GTAC	1 cut(s)	685
DdeI	CTNAG	6 cut(s)	129, 375, 649, 714, 810, 950
DpnI	GATC	6 cut(s)	71, 165, 315, 448, 512, 671
DpnII	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
DraIII	CACNNNGTG	1 cut(s)	706
Eam1104I	CTCTTC	1 cut(s)	39
EarI	CTCTTC	1 cut(s)	39
Eco130I	CCWWGG	6 cut(s)	60, 115, 180, 450, 471, 885
Eco147I	AGGCCT	1 cut(s)	219
Eco24I	GRGCYC	1 cut(s)	529
Eco32I	GATATC	1 cut(s)	123
Eco47I	GGWCC	3 cut(s)	28, 455, 468
Eco57I	CTGAAG	1 cut(s)	449
Eco88I	CYCGRG	3 cut(s)	317, 757, 942
Eco91I	GGTNACC	1 cut(s)	653
EcoNI	CCTNNNNNAGG	1 cut(s)	512
EcoO65I	GGTNACC	1 cut(s)	653
EcoRII	CCWGG	2 cut(s)	561, 914
EcoRV	GATATC	1 cut(s)	123
EcoT14I	CCWWGG	6 cut(s)	60, 115, 180, 450, 471, 885
EcoT22I	ATGCAT	1 cut(s)	82
EcoT38I	GRGCYC	1 cut(s)	529
ErhI	CCWWGG	6 cut(s)	60, 115, 180, 450, 471, 885
FaeI	CATG	7 cut(s)	28, 84, 119, 184, 343, 495, 856
FaqI	GGGAC	4 cut(s)	480, 481, 531, 602
FatI	CATG	7 cut(s)	24, 80, 115, 180, 339, 491, 852
FbaI	TGATCA	2 cut(s)	510, 669
Fnu4HI	GCNGC	3 cut(s)	54, 210, 580
FokI	GGATG	5 cut(s)	536, 578, 611, 622, 626
FriOI	GRGCYC	1 cut(s)	529
Fsp4HI	GCNGC	3 cut(s)	54, 210, 580
FspBI	CTAG	1 cut(s)	371
GlaI	GCGC	1 cut(s)	278
GluI	GCNGC	3 cut(s)	54, 210, 580
GsuI	CTGGAG	2 cut(s)	569, 818
HaeIII	GGCC	5 cut(s)	219, 527, 582, 870, 914
HapII	CCGG	5 cut(s)	523, 703, 758, 782, 788
HgaI	GACGC	1 cut(s)	545
HhaI	GCGC	1 cut(s)	279
Hin1II	CATG	7 cut(s)	28, 84, 119, 184, 343, 495, 856
Hin6I	GCGC	1 cut(s)	277
HinP1I	GCGC	1 cut(s)	277
HincII	GTYRAC	1 cut(s)	22
HindII	GTYRAC	1 cut(s)	22
HindIII	AAGCTT	1 cut(s)	380
HinfI	GANTC	2 cut(s)	403, 519
HpaII	CCGG	5 cut(s)	523, 703, 758, 782, 788
HphI	GGTGA	7 cut(s)	53, 121, 614, 664, 665, 679, 718
Hpy166II	GTNNAC	3 cut(s)	22, 112, 628
Hpy188I	TCNGA	8 cut(s)	238, 304, 429, 459, 721, 811, 951, 962
Hpy188III	TCNNGA	1 cut(s)	317
Hpy8I	GTNNAC	3 cut(s)	22, 112, 628
Hpy99I	CGWCG	2 cut(s)	539, 767
HpyAV	CCTTC	7 cut(s)	277, 293, 297, 337, 412, 427, 904
HpyCH4III	ACNGT	1 cut(s)	145
HpyCH4V	TGCA	4 cut(s)	80, 353, 628, 700
HpyF10VI	GCNNNNNNNGC	1 cut(s)	911
HpyF3I	CTNAG	6 cut(s)	129, 375, 649, 714, 810, 950
Hsp92II	CATG	7 cut(s)	28, 84, 119, 184, 343, 495, 856
HspAI	GCGC	1 cut(s)	277
Ksp22I	TGATCA	2 cut(s)	510, 669
Kzo9I	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
LmnI	GCTCC	3 cut(s)	184, 594, 889
Lsp1109I	GCAGC	2 cut(s)	65, 221
LweI	GCATC	2 cut(s)	67, 757
MaeI	CTAG	1 cut(s)	371
MaeIII	GTNAC	1 cut(s)	653
MalI	GATC	6 cut(s)	71, 165, 315, 448, 512, 671
MboI	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
MboII	GAAGA	9 cut(s)	56, 79, 163, 187, 262, 335, 346, 442, 814
MflI	RGATCY	1 cut(s)	446
MhlI	GDGCHC	2 cut(s)	529, 630
MluCI	AATT	6 cut(s)	347, 359, 460, 833, 875, 988
MlyI	GAGTC	1 cut(s)	513
MmeI	TCCRAC	1 cut(s)	699
Mph1103I	ATGCAT	1 cut(s)	82
MroXI	GAANNNNTTC	1 cut(s)	437
MspA1I	CMGCKG	1 cut(s)	579
MspI	CCGG	5 cut(s)	523, 703, 758, 782, 788
MspR9I	CCNGG	7 cut(s)	524, 563, 704, 758, 759, 782, 916
MvaI	CCWGG	2 cut(s)	563, 916
MwoI	GCNNNNNNNGC	1 cut(s)	911
NciI	CCSGG	5 cut(s)	524, 704, 758, 759, 782
NcoI	CCATGG	2 cut(s)	115, 180
NdeII	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
NheI	GCTAGC	1 cut(s)	370
NlaIII	CATG	7 cut(s)	28, 84, 119, 184, 343, 495, 856
NlaIV	GGNNCC	7 cut(s)	448, 456, 469, 527, 727, 786, 891
NmuCI	GTSAC	1 cut(s)	653
NsiI	ATGCAT	1 cut(s)	82
NspI	RCATGY	1 cut(s)	84
PaeR7I	CTCGAG	1 cut(s)	317
PceI	AGGCCT	1 cut(s)	219
PcsI	WCGNNNNNNNCGW	1 cut(s)	743
PdmI	GAANNNNTTC	1 cut(s)	437
PfeI	GAWTC	1 cut(s)	403
PflFI	GACNNNGTC	1 cut(s)	572
PflMI	CCANNNNNTGG	1 cut(s)	250
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	3 cut(s)	55, 211, 581
PleI	GAGTC	1 cut(s)	513
PpsI	GAGTC	1 cut(s)	513
PshAI	GACNNNNGTC	1 cut(s)	473
Psp6I	CCWGG	2 cut(s)	561, 914
PspEI	GGTNACC	1 cut(s)	653
PspGI	CCWGG	2 cut(s)	561, 914
PspN4I	GGNNCC	7 cut(s)	448, 456, 469, 527, 727, 786, 891
PspOMI	GGGCCC	1 cut(s)	525
PspPI	GGNCC	5 cut(s)	28, 455, 468, 525, 526
PsuI	RGATCY	1 cut(s)	446
PsyI	GACNNNGTC	1 cut(s)	572
RsaI	GTAC	1 cut(s)	686
RsaNI	GTAC	1 cut(s)	685
SatI	GCNGC	3 cut(s)	54, 210, 580
Sau3AI	GATC	6 cut(s)	69, 163, 313, 446, 510, 669
Sau96I	GGNCC	5 cut(s)	28, 455, 468, 525, 526
SchI	GAGTC	1 cut(s)	513
ScrFI	CCNGG	7 cut(s)	524, 563, 704, 758, 759, 782, 916
SduI	GDGCHC	2 cut(s)	529, 630
SfaNI	GCATC	2 cut(s)	67, 757
Sfr274I	CTCGAG	1 cut(s)	317
SinI	GGWCC	3 cut(s)	28, 455, 468
SlaI	CTCGAG	1 cut(s)	317
SmaI	CCCGGG	1 cut(s)	759
SmlI	CTYRAG	3 cut(s)	317, 413, 593
SmoI	CTYRAG	3 cut(s)	317, 413, 593
Sse9I	AATT	6 cut(s)	347, 359, 460, 833, 875, 988
SseBI	AGGCCT	1 cut(s)	219
SsiI	CCGC	1 cut(s)	579
SspMI	CTAG	1 cut(s)	371
StuI	AGGCCT	1 cut(s)	219
StyD4I	CCNGG	7 cut(s)	522, 561, 702, 756, 757, 780, 914
StyI	CCWWGG	6 cut(s)	60, 115, 180, 450, 471, 885
TaaI	ACNGT	1 cut(s)	145
TaqI	TCGA	3 cut(s)	222, 318, 737
TasI	AATT	6 cut(s)	347, 359, 460, 833, 875, 988
TatI	WGTACW	1 cut(s)	684
TauI	GCSGC	1 cut(s)	582
TfiI	GAWTC	1 cut(s)	403
TscAI	CASTG	3 cut(s)	148, 360, 630
TseFI	GTSAC	1 cut(s)	653
TseI	GCWGC	2 cut(s)	53, 209
Tsp45I	GTSAC	1 cut(s)	653
TspDTI	ATGAA	4 cut(s)	170, 356, 869, 923
TspGWI	ACGGA	1 cut(s)	738
TspMI	CCCGGG	1 cut(s)	757
TspRI	CASTG	3 cut(s)	148, 360, 630
Tth111I	GACNNNGTC	1 cut(s)	572
Van91I	CCANNNNNTGG	1 cut(s)	250
VneI	GTGCAC	1 cut(s)	626
VpaK11BI	GGWCC	3 cut(s)	28, 455, 468
XagI	CCTNNNNNAGG	1 cut(s)	512
XapI	RAATTY	4 cut(s)	347, 460, 833, 875
XceI	RCATGY	1 cut(s)	84
XcmI	CCANNNNNNNNNTGG	1 cut(s)	536
XhoI	CTCGAG	1 cut(s)	317
XmaI	CCCGGG	1 cut(s)	757
XmnI	GAANNNNTTC	1 cut(s)	437
XspI	CTAG	1 cut(s)	371
Zsp2I	ATGCAT	1 cut(s)	82

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.