RLG00000030975

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013988
Physical Location & Seq
Reverse (-)
993347 .. 994916
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030975

Sequence Viewer

Length: 936 bp
ATGGAGAATTTCCCGGTTGTTAACATGGAGCAACTCAACGGTGAAGAGAGACAAGCAACCATGGAGAAGATAAACGATGCTTGTGAGAATTGGGGTTTCTTTGAGTTGGTGAACCATGGGATATCCCATGAGCTGATGGACAAAGTAGAGAAGCTCACAAAGGAGCACTACAGAAAGTGCATGGAGCAAAGGTTCAAGGAAATGGTGGCAAGCAAAGGCCTCGAATCTGTCAACTCTGAAATCGAAGATTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTCCCCTTCTCGAACATTTCCCAAACCGATCTCGACGAAGATTACAGGGAGGCCATGAAGGAATTTGCAGTGGAACTAGAGAAACTGGCTGAGCAACTGTTGGACTTGCTGTGTGAGAATCTGGGGCTGGATAAGGGTTACCTGAAGAAGGCTTTCTATGGATCCGAGGGACCAAATTTTGGGACCAAGGTGAGCAACTACCCTCCATGCCCCAAACCGGACCTGATCAAGGGACTCCGGGCCCACACCGACGCCGGTGGCATCATCCTACTATTCCAAGATGACAAGGTCAGCGGCCTCCAGCTCCTCAAGGATGGCCAATGGATTGATGTGCCCCCAATGCACCACTCCATTGTCATTAACTTAGGTGACCAACTTGAGGTGATCACTAATGGCAAGTACAAGAGTGTGATGCACCGTGTGATAGCGCAACCTGATGGAAACAGAATGTCGATAGCCTCGTTCTACAACCCAGGCAATGATGCTTTTATCTCTCCAGCACCAGCAATGCTTGAAAAACAAACTGAGGAATGCCCAACTTATCCAAAATTTGTGTTTGATGACTACATGAAGCTCTACTCAGGCCTCAAATTCCAAGCCAAGGAGCCGAGGTTTGAAGCTATGAAGGCTATGGAATCAATTGCAACTGTTTGA

Protein Analysis

312

Amino Acids

35.52

Weight (kDa)

4.95

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 5 - 96 3.5e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 158 - 252 1.7e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12010 AT1G62380
fragaria_vesca FvH4_3g01280
malus_domestica MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica Prupe.4G013800_v2.0.a1
pyrus_communis pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis RchiOBHm_Chr5g0001831
rosa_laevigata RLG00000030975
rosa_multiflora Rmu_ssc0000237.1_g000010
rosa_roxburghii Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa Rorug04G0393500
rosa_samantha Rh5DG016100
rosa_wichuraiana Rw5G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 461
AciI CCGC 1 cut(s) 576
AclWI GGATC 2 cut(s) 438, 451
AcoI YGGCCR 1 cut(s) 598
AcsI RAATTY 5 cut(s) 7, 344, 457, 830, 872
AcuI CTGAAG 1 cut(s) 446
AcyI GRCGYC 1 cut(s) 534
AdeI CACNNNGTG 1 cut(s) 703
AfaI GTAC 1 cut(s) 683
AfiI CCNNNNNNNGG 6 cut(s) 430, 461, 499, 511, 661, 883
AgsI TTSAA 3 cut(s) 196, 797, 899
AjnI CCWGG 1 cut(s) 754
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 5 cut(s) 133, 154, 586, 856, 902
AluI AGCT 5 cut(s) 133, 154, 586, 856, 902
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 2 cut(s) 438, 451
AoxI GGCC 6 cut(s) 217, 333, 522, 577, 598, 865
ApaI GGGCCC 1 cut(s) 526
ApoI RAATTY 5 cut(s) 7, 344, 457, 830, 872
Asp700I GAANNNNTTC 1 cut(s) 434
AspLEI GCGC 2 cut(s) 279, 712
AspS9I GGNCC 5 cut(s) 452, 465, 502, 522, 523
AsuC2I CCSGG 2 cut(s) 14, 521
AsuHPI GGTGA 5 cut(s) 53, 121, 484, 662, 676
AvaII GGWCC 3 cut(s) 452, 465, 502
BaeGI GKGCMC 2 cut(s) 526, 618
BalI TGGCCA 1 cut(s) 600
BamHI GGATCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 526
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 3 cut(s) 130, 590, 713
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 1 cut(s) 756
BclI TGATCA 2 cut(s) 507, 666
BcnI CCSGG 2 cut(s) 14, 521
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 1 cut(s) 577
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 578
Bme1390I CCNGG 3 cut(s) 14, 521, 756
Bme18I GGWCC 3 cut(s) 452, 465, 502
BmgT120I GGNCC 5 cut(s) 452, 465, 502, 522, 523
BmiI GGNNCC 5 cut(s) 445, 453, 466, 524, 888
BmrFI CCNGG 3 cut(s) 14, 521, 756
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 4 cut(s) 67, 552, 684, 754
BmuI ACTGGG 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 566, 762
Bpu1102I GCTNAGC 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 575, 680
BpuMI CCSGG 2 cut(s) 14, 521
BsaHI GRCGYC 1 cut(s) 534
BsaJI CCNNGG 7 cut(s) 60, 115, 447, 468, 754, 882, 890
BsaWI WCCGGW 1 cut(s) 499
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 878, 908
Bsc4I CCNNNNNNNGG 6 cut(s) 430, 461, 499, 511, 661, 883
Bse118I RCCGGY 1 cut(s) 536
Bse1I ACTGG 2 cut(s) 260, 372
Bse3DI GCAATG 2 cut(s) 766, 795
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 7 cut(s) 60, 115, 447, 468, 754, 882, 890
BseGI GGATG 2 cut(s) 546, 601
BseLI CCNNNNNNNGG 6 cut(s) 430, 461, 499, 511, 661, 883
BseMI GCAATG 2 cut(s) 766, 795
BseMII CTCAG 3 cut(s) 363, 798, 876
BseNI ACTGG 2 cut(s) 260, 372
BseRI GAGGAG 1 cut(s) 578
BseSI GKGCMC 2 cut(s) 526, 618
BshFI GGCC 6 cut(s) 219, 335, 524, 579, 600, 867
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 4 cut(s) 14, 500, 520, 537
BslFI GGGAC 3 cut(s) 465, 478, 528
BslI CCNNNNNNNGG 6 cut(s) 430, 461, 499, 511, 661, 883
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 3 cut(s) 465, 478, 528
BsmI GAATGC 1 cut(s) 818
BsnI GGCC 6 cut(s) 219, 335, 524, 579, 600, 867
Bsp120I GGGCCC 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 168, 526, 618
Bsp143I GATC 4 cut(s) 310, 443, 507, 666
Bsp1720I GCTNAGC 1 cut(s) 372
Bsp19I CCATGG 2 cut(s) 60, 115
BspACI CCGC 1 cut(s) 576
BspANI GGCC 6 cut(s) 219, 335, 524, 579, 600, 867
BspCNI CTCAG 3 cut(s) 364, 799, 875
BspLI GGNNCC 5 cut(s) 445, 453, 466, 524, 888
BspPI GGATC 2 cut(s) 438, 451
BsrDI GCAATG 2 cut(s) 766, 795
BsrFI RCCGGY 1 cut(s) 536
BsrI ACTGG 2 cut(s) 260, 372
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 7 cut(s) 60, 115, 447, 468, 754, 882, 890
BssMI GATC 4 cut(s) 310, 443, 507, 666
BssNI GRCGYC 1 cut(s) 534
BssT1I CCWWGG 4 cut(s) 60, 115, 468, 882
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 41, 381, 701, 931
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 4 cut(s) 372, 646, 807, 862
BstDSI CCRYGG 2 cut(s) 60, 115
BstEII GGTNACC 2 cut(s) 419, 650
BstENI CCTNNNNNAGG 2 cut(s) 428, 509
BstF5I GGATG 2 cut(s) 546, 601
BstHHI GCGC 2 cut(s) 279, 712
BstKTI GATC 4 cut(s) 313, 446, 510, 669
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 4 cut(s) 310, 443, 507, 666
BstMWI GCNNNNNNNGC 2 cut(s) 622, 908
BstNI CCWGG 1 cut(s) 756
BstPI GGTNACC 2 cut(s) 419, 650
BstSCI CCNGG 3 cut(s) 12, 519, 754
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 2 cut(s) 526, 618
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BsuRI GGCC 6 cut(s) 219, 335, 524, 579, 600, 867
BtgI CCRYGG 2 cut(s) 60, 115
BtsCI GGATG 2 cut(s) 546, 601
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 211
CfoI GCGC 2 cut(s) 279, 712
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 5 cut(s) 452, 465, 502, 522, 523
CseI GACGC 1 cut(s) 542
Csp6I GTAC 1 cut(s) 682
CviAII CATG 8 cut(s) 25, 61, 116, 128, 181, 337, 489, 850
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 4 cut(s) 372, 646, 807, 862
DpnI GATC 4 cut(s) 312, 445, 509, 668
DpnII GATC 4 cut(s) 310, 443, 507, 666
DraIII CACNNNGTG 1 cut(s) 703
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 4 cut(s) 60, 115, 468, 882
Eco147I AGGCCT 2 cut(s) 219, 867
Eco24I GRGCYC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 452, 465, 502
Eco57I CTGAAG 1 cut(s) 446
Eco91I GGTNACC 2 cut(s) 419, 650
EcoNI CCTNNNNNAGG 2 cut(s) 428, 509
EcoO65I GGTNACC 2 cut(s) 419, 650
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 4 cut(s) 60, 115, 468, 882
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 4 cut(s) 60, 115, 468, 882
FaeI CATG 8 cut(s) 28, 64, 119, 131, 184, 340, 492, 853
FaqI GGGAC 3 cut(s) 465, 478, 528
FatI CATG 8 cut(s) 24, 60, 115, 127, 180, 336, 488, 849
FbaI TGATCA 2 cut(s) 507, 666
Fnu4HI GCNGC 1 cut(s) 577
FokI GGATG 2 cut(s) 533, 608
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 577
FspBI CTAG 1 cut(s) 359
GlaI GCGC 2 cut(s) 278, 711
GluI GCNGC 1 cut(s) 577
GsuI CTGGAG 2 cut(s) 566, 762
HaeIII GGCC 6 cut(s) 219, 335, 524, 579, 600, 867
HapII CCGG 4 cut(s) 14, 500, 520, 537
HgaI GACGC 1 cut(s) 542
HhaI GCGC 2 cut(s) 279, 712
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 8 cut(s) 28, 64, 119, 131, 184, 340, 492, 853
Hin6I GCGC 2 cut(s) 277, 710
HinP1I GCGC 2 cut(s) 277, 710
HincII GTYRAC 2 cut(s) 22, 232
HindII GTYRAC 2 cut(s) 22, 232
HinfI GANTC 4 cut(s) 224, 400, 516, 917
HpaI GTTAAC 1 cut(s) 22
HpaII CCGG 4 cut(s) 14, 500, 520, 537
HphI GGTGA 5 cut(s) 53, 121, 484, 662, 676
Hpy166II GTNNAC 3 cut(s) 22, 112, 232
Hpy188I TCNGA 2 cut(s) 238, 448
Hpy188III TCNNGA 2 cut(s) 292, 314
Hpy8I GTNNAC 3 cut(s) 22, 112, 232
Hpy99I CGWCG 2 cut(s) 320, 536
HpyAV CCTTC 5 cut(s) 277, 298, 334, 424, 901
HpyCH4III ACNGT 4 cut(s) 41, 381, 701, 931
HpyCH4V TGCA 5 cut(s) 180, 350, 625, 697, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 622, 908
HpyF3I CTNAG 4 cut(s) 372, 646, 807, 862
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 8 cut(s) 28, 64, 119, 131, 184, 340, 492, 853
HspAI GCGC 2 cut(s) 277, 710
Ksp22I TGATCA 2 cut(s) 507, 666
KspAI GTTAAC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 310, 443, 507, 666
LmnI GCTCC 5 cut(s) 28, 163, 184, 591, 886
LweI GCATC 4 cut(s) 67, 552, 684, 754
MaeI CTAG 1 cut(s) 359
MaeIII GTNAC 2 cut(s) 419, 650
MalI GATC 4 cut(s) 312, 445, 509, 668
MboI GATC 4 cut(s) 310, 443, 507, 666
MboII GAAGA 6 cut(s) 56, 79, 257, 262, 332, 439
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 443
MhlI GDGCHC 3 cut(s) 168, 526, 618
MlsI TGGCCA 1 cut(s) 600
MluCI AATT 7 cut(s) 7, 88, 344, 457, 830, 872, 921
MluNI TGGCCA 1 cut(s) 600
MlyI GAGTC 1 cut(s) 510
MmeI TCCRAC 1 cut(s) 363
Mox20I TGGCCA 1 cut(s) 600
MroXI GAANNNNTTC 1 cut(s) 434
MscI TGGCCA 1 cut(s) 600
MseI TTAA 2 cut(s) 21, 642
Msp20I TGGCCA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 4 cut(s) 14, 500, 520, 537
MspR9I CCNGG 3 cut(s) 14, 521, 756
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 818
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 2 cut(s) 622, 908
NciI CCSGG 2 cut(s) 14, 521
NcoI CCATGG 2 cut(s) 60, 115
NdeII GATC 4 cut(s) 310, 443, 507, 666
NlaIII CATG 8 cut(s) 28, 64, 119, 131, 184, 340, 492, 853
NlaIV GGNNCC 5 cut(s) 445, 453, 466, 524, 888
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 1 cut(s) 650
PceI AGGCCT 2 cut(s) 219, 867
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PctI GAATGC 1 cut(s) 818
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 3 cut(s) 224, 400, 917
PflFI GACNNNGTC 1 cut(s) 569
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 1 cut(s) 578
PleI GAGTC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 754
PspEI GGTNACC 2 cut(s) 419, 650
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 5 cut(s) 445, 453, 466, 524, 888
PspOMI GGGCCC 1 cut(s) 522
PspPI GGNCC 5 cut(s) 452, 465, 502, 522, 523
PsuI RGATCY 1 cut(s) 443
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
SaqAI TTAA 2 cut(s) 21, 642
SatI GCNGC 1 cut(s) 577
Sau3AI GATC 4 cut(s) 310, 443, 507, 666
Sau96I GGNCC 5 cut(s) 452, 465, 502, 522, 523
SchI GAGTC 1 cut(s) 510
ScrFI CCNGG 3 cut(s) 14, 521, 756
SduI GDGCHC 3 cut(s) 168, 526, 618
SfaNI GCATC 4 cut(s) 67, 552, 684, 754
SfcI CTRYAG 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 536
SinI GGWCC 3 cut(s) 452, 465, 502
SmlI CTYRAG 2 cut(s) 590, 659
SmoI CTYRAG 2 cut(s) 590, 659
Sse9I AATT 7 cut(s) 7, 88, 344, 457, 830, 872, 921
SseBI AGGCCT 2 cut(s) 219, 867
SsiI CCGC 1 cut(s) 576
SspMI CTAG 1 cut(s) 359
StuI AGGCCT 2 cut(s) 219, 867
StyD4I CCNGG 3 cut(s) 12, 519, 754
StyI CCWWGG 4 cut(s) 60, 115, 468, 882
TaaI ACNGT 4 cut(s) 41, 381, 701, 931
TaqI TCGA 5 cut(s) 222, 243, 293, 315, 734
TasI AATT 7 cut(s) 7, 88, 344, 457, 830, 872, 921
TatI WGTACW 1 cut(s) 681
TauI GCSGC 1 cut(s) 579
TfiI GAWTC 3 cut(s) 224, 400, 917
Tru1I TTAA 2 cut(s) 21, 642
Tru9I TTAA 2 cut(s) 21, 642
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 650
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 3 cut(s) 353, 866, 920
TspRI CASTG 1 cut(s) 357
Tth111I GACNNNGTC 1 cut(s) 569
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 3 cut(s) 452, 465, 502
XagI CCTNNNNNAGG 2 cut(s) 428, 509
XapI RAATTY 5 cut(s) 7, 344, 457, 830, 872
XmnI GAANNNNTTC 1 cut(s) 434
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.