Rroxscaffold_1G00071260

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013988
Physical Location & Seq
Reverse (-)
92134309 .. 92136296
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071260.1

Sequence Viewer

Length: 939 bp
ATGGAGAATTTCCCGGTTTTTAACATGGAGCAACTCAACGGTGAAAAGAGACAAGCAACCATGGAGAAGATAAACGATGCTTGTGAGAATTGGGGTTTCTTTGAGTTGGTGAACCATGGGATATCCCATGAGCTGATGGACAAGGTAGAGAAGCTCACAAAGGAGCACTACAGAAAGTGCATGGAGCAAAGGTTCAAGGAAATGGTGGCAAGCAAAGGCCTCGAATCTGTCAACTCTGAAATCGAAGATTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTCCCCTCCTCCAACATTTCCCAAATCCCCGATCTCGACGAAGATTACAGGGAGGCCATGAAGGAATTTGCAGTGGAACTAGAGAAACTGGCTGAGCAACTGTTGGACTTGCTGTGTGAGAATCTGGGGCTGGATAAGGGTTACCTGAAGAAAGCTTTCTATGGATCCGAGGGACCAAATTTTGGGACCAAGGTGAGCAACTACCCTCCATGCCCCAAACCGGACCTGATCAAGGGACTCCGGGCCCACACCGACGCCGGTGGCATCATCCTACTATTCCAAGATGACAAGGTCAGCGGCCTCCAGCTCCTCAAGGATGACCAATGGATTGATGTGCCCCCAATGCACCACTCCATTGTCATCAACTTAGGTGACCAACTTGAGGTGATCACTAATGGCAAGTACAAGAGTGTGATGCACCGTGTGATAGCGCAACCTGATGGAAACAGAATGTCGATAGCTTCGTTCTACAACCCAGGCAATGATGCTTTTATCTCTCCAGCACCGGCAATGCTTGAAAAACAAACTGAGGAATGCCCAACTTATCCAAAATTTGTGTTTGATGACTACATGAAGCTCTATTCAGGCCTCAAATTCCAAGCCAAGGAGCCGAGGTTTGAAGCTATGAAGGCTATGGAATCAATTGCAACTGTTTGA

Protein Analysis

312

Amino Acids

35.68

Weight (kDa)

5.0

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 5 - 97 7.8e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 159 - 253 4.4e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12010 AT1G62380
fragaria_vesca FvH4_3g01280
malus_domestica MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica Prupe.4G013800_v2.0.a1
pyrus_communis pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis RchiOBHm_Chr5g0001831
rosa_laevigata RLG00000030975
rosa_multiflora Rmu_ssc0000237.1_g000010
rosa_roxburghii Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa Rorug04G0393500
rosa_samantha Rh5DG016100
rosa_wichuraiana Rw5G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 1 cut(s) 579
AclWI GGATC 2 cut(s) 441, 454
AcsI RAATTY 5 cut(s) 7, 347, 460, 833, 875
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 537
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 5 cut(s) 464, 502, 514, 664, 886
AgsI TTSAA 3 cut(s) 196, 800, 902
AjnI CCWGG 1 cut(s) 757
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 7 cut(s) 133, 154, 437, 589, 743, 859, 905
AluI AGCT 7 cut(s) 133, 154, 437, 589, 743, 859, 905
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 2 cut(s) 441, 454
AoxI GGCC 5 cut(s) 217, 336, 525, 580, 868
ApaI GGGCCC 1 cut(s) 529
ApoI RAATTY 5 cut(s) 7, 347, 460, 833, 875
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 2 cut(s) 279, 715
AspS9I GGNCC 5 cut(s) 455, 468, 505, 525, 526
AsuC2I CCSGG 2 cut(s) 14, 524
AsuHPI GGTGA 5 cut(s) 53, 121, 487, 665, 679
AvaII GGWCC 3 cut(s) 455, 468, 505
BaeGI GKGCMC 2 cut(s) 529, 621
BamHI GGATCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 2 cut(s) 130, 716
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 1 cut(s) 759
BclI TGATCA 2 cut(s) 510, 669
BcnI CCSGG 2 cut(s) 14, 524
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 1 cut(s) 580
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 581
Bme1390I CCNGG 3 cut(s) 14, 524, 759
Bme18I GGWCC 3 cut(s) 455, 468, 505
BmgT120I GGNCC 5 cut(s) 455, 468, 505, 525, 526
BmiI GGNNCC 5 cut(s) 448, 456, 469, 527, 891
BmrFI CCNGG 3 cut(s) 14, 524, 759
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 4 cut(s) 67, 555, 687, 757
BmuI ACTGGG 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 569, 765
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 578, 683
BpuMI CCSGG 2 cut(s) 14, 524
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 7 cut(s) 60, 115, 450, 471, 757, 885, 893
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 272, 302, 881, 911
Bsc4I CCNNNNNNNGG 5 cut(s) 464, 502, 514, 664, 886
Bse118I RCCGGY 2 cut(s) 539, 787
Bse1I ACTGG 2 cut(s) 260, 375
Bse3DI GCAATG 2 cut(s) 769, 798
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 7 cut(s) 60, 115, 450, 471, 757, 885, 893
BseGI GGATG 2 cut(s) 549, 604
BseLI CCNNNNNNNGG 5 cut(s) 464, 502, 514, 664, 886
BseMI GCAATG 2 cut(s) 769, 798
BseMII CTCAG 2 cut(s) 366, 801
BseNI ACTGG 2 cut(s) 260, 375
BseRI GAGGAG 2 cut(s) 280, 581
BseSI GKGCMC 2 cut(s) 529, 621
BshFI GGCC 5 cut(s) 219, 338, 527, 582, 870
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 5 cut(s) 14, 503, 523, 540, 788
BslFI GGGAC 3 cut(s) 468, 481, 531
BslI CCNNNNNNNGG 5 cut(s) 464, 502, 514, 664, 886
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 3 cut(s) 468, 481, 531
BsmI GAATGC 1 cut(s) 821
BsnI GGCC 5 cut(s) 219, 338, 527, 582, 870
Bsp120I GGGCCC 1 cut(s) 525
Bsp1286I GDGCHC 3 cut(s) 168, 529, 621
Bsp143I GATC 4 cut(s) 313, 446, 510, 669
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 2 cut(s) 60, 115
BspACI CCGC 1 cut(s) 579
BspANI GGCC 5 cut(s) 219, 338, 527, 582, 870
BspCNI CTCAG 2 cut(s) 367, 802
BspLI GGNNCC 5 cut(s) 448, 456, 469, 527, 891
BspPI GGATC 2 cut(s) 441, 454
BsrDI GCAATG 2 cut(s) 769, 798
BsrFI RCCGGY 2 cut(s) 539, 787
BsrI ACTGG 2 cut(s) 260, 375
BssAI RCCGGY 2 cut(s) 539, 787
BssECI CCNNGG 7 cut(s) 60, 115, 450, 471, 757, 885, 893
BssMI GATC 4 cut(s) 313, 446, 510, 669
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 4 cut(s) 60, 115, 471, 885
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 4 cut(s) 41, 384, 704, 934
BstACI GRCGYC 1 cut(s) 537
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 3 cut(s) 375, 649, 810
BstDSI CCRYGG 2 cut(s) 60, 115
BstEII GGTNACC 2 cut(s) 422, 653
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 2 cut(s) 549, 604
BstHHI GCGC 2 cut(s) 279, 715
BstKTI GATC 4 cut(s) 316, 449, 513, 672
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 4 cut(s) 313, 446, 510, 669
BstMWI GCNNNNNNNGC 2 cut(s) 625, 911
BstNI CCWGG 1 cut(s) 759
BstPI GGTNACC 2 cut(s) 422, 653
BstSCI CCNGG 3 cut(s) 12, 522, 757
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 2 cut(s) 529, 621
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 5 cut(s) 219, 338, 527, 582, 870
BtgI CCRYGG 2 cut(s) 60, 115
BtsCI GGATG 2 cut(s) 549, 604
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 211
CfoI GCGC 2 cut(s) 279, 715
Cfr10I RCCGGY 2 cut(s) 539, 787
Cfr13I GGNCC 5 cut(s) 455, 468, 505, 525, 526
CseI GACGC 1 cut(s) 545
Csp6I GTAC 1 cut(s) 685
CviAII CATG 8 cut(s) 25, 61, 116, 128, 181, 340, 492, 853
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 3 cut(s) 375, 649, 810
DpnI GATC 4 cut(s) 315, 448, 512, 671
DpnII GATC 4 cut(s) 313, 446, 510, 669
DraIII CACNNNGTG 1 cut(s) 706
Eco130I CCWWGG 4 cut(s) 60, 115, 471, 885
Eco147I AGGCCT 2 cut(s) 219, 870
Eco24I GRGCYC 1 cut(s) 529
Eco32I GATATC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 455, 468, 505
Eco57I CTGAAG 1 cut(s) 449
Eco91I GGTNACC 2 cut(s) 422, 653
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO65I GGTNACC 2 cut(s) 422, 653
EcoRII CCWGG 1 cut(s) 757
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 4 cut(s) 60, 115, 471, 885
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 4 cut(s) 60, 115, 471, 885
FaeI CATG 8 cut(s) 28, 64, 119, 131, 184, 343, 495, 856
FaqI GGGAC 3 cut(s) 468, 481, 531
FatI CATG 8 cut(s) 24, 60, 115, 127, 180, 339, 491, 852
FbaI TGATCA 2 cut(s) 510, 669
Fnu4HI GCNGC 1 cut(s) 580
FokI GGATG 2 cut(s) 536, 611
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 1 cut(s) 580
FspBI CTAG 1 cut(s) 362
GlaI GCGC 2 cut(s) 278, 714
GluI GCNGC 1 cut(s) 580
GsuI CTGGAG 2 cut(s) 569, 765
HaeIII GGCC 5 cut(s) 219, 338, 527, 582, 870
HapII CCGG 5 cut(s) 14, 503, 523, 540, 788
HgaI GACGC 1 cut(s) 545
HhaI GCGC 2 cut(s) 279, 715
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 8 cut(s) 28, 64, 119, 131, 184, 343, 495, 856
Hin6I GCGC 2 cut(s) 277, 713
HinP1I GCGC 2 cut(s) 277, 713
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 4 cut(s) 224, 403, 519, 920
HpaII CCGG 5 cut(s) 14, 503, 523, 540, 788
HphI GGTGA 5 cut(s) 53, 121, 487, 665, 679
Hpy166II GTNNAC 2 cut(s) 112, 232
Hpy188I TCNGA 2 cut(s) 238, 451
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 2 cut(s) 112, 232
Hpy99I CGWCG 2 cut(s) 323, 539
HpyAV CCTTC 3 cut(s) 277, 337, 904
HpyCH4III ACNGT 4 cut(s) 41, 384, 704, 934
HpyCH4V TGCA 5 cut(s) 180, 353, 628, 700, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 625, 911
HpyF3I CTNAG 3 cut(s) 375, 649, 810
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 8 cut(s) 28, 64, 119, 131, 184, 343, 495, 856
HspAI GCGC 2 cut(s) 277, 713
Ksp22I TGATCA 2 cut(s) 510, 669
Kzo9I GATC 4 cut(s) 313, 446, 510, 669
LmnI GCTCC 5 cut(s) 28, 163, 184, 594, 889
LweI GCATC 4 cut(s) 67, 555, 687, 757
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 422, 653
MalI GATC 4 cut(s) 315, 448, 512, 671
MboI GATC 4 cut(s) 313, 446, 510, 669
MboII GAAGA 5 cut(s) 79, 257, 262, 335, 442
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 3 cut(s) 168, 529, 621
MluCI AATT 7 cut(s) 7, 88, 347, 460, 833, 875, 924
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 2 cut(s) 318, 366
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 579
MspI CCGG 5 cut(s) 14, 503, 523, 540, 788
MspR9I CCNGG 3 cut(s) 14, 524, 759
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 821
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 2 cut(s) 625, 911
NciI CCSGG 2 cut(s) 14, 524
NcoI CCATGG 2 cut(s) 60, 115
NdeII GATC 4 cut(s) 313, 446, 510, 669
NlaIII CATG 8 cut(s) 28, 64, 119, 131, 184, 343, 495, 856
NlaIV GGNNCC 5 cut(s) 448, 456, 469, 527, 891
NmeAIII GCCGAG 1 cut(s) 918
NmuCI GTSAC 1 cut(s) 653
PceI AGGCCT 2 cut(s) 219, 870
PcsI WCGNNNNNNNCGW 1 cut(s) 743
PctI GAATGC 1 cut(s) 821
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 224, 403, 920
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 1 cut(s) 581
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 757
PspEI GGTNACC 2 cut(s) 422, 653
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 5 cut(s) 448, 456, 469, 527, 891
PspOMI GGGCCC 1 cut(s) 525
PspPI GGNCC 5 cut(s) 455, 468, 505, 525, 526
PsuI RGATCY 1 cut(s) 446
PsyI GACNNNGTC 1 cut(s) 572
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 580
Sau3AI GATC 4 cut(s) 313, 446, 510, 669
Sau96I GGNCC 5 cut(s) 455, 468, 505, 525, 526
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 3 cut(s) 14, 524, 759
SduI GDGCHC 3 cut(s) 168, 529, 621
SfaNI GCATC 4 cut(s) 67, 555, 687, 757
SfcI CTRYAG 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 539
SinI GGWCC 3 cut(s) 455, 468, 505
SmlI CTYRAG 2 cut(s) 593, 662
SmoI CTYRAG 2 cut(s) 593, 662
Sse9I AATT 7 cut(s) 7, 88, 347, 460, 833, 875, 924
SseBI AGGCCT 2 cut(s) 219, 870
SsiI CCGC 1 cut(s) 579
SspMI CTAG 1 cut(s) 362
StuI AGGCCT 2 cut(s) 219, 870
StyD4I CCNGG 3 cut(s) 12, 522, 757
StyI CCWWGG 4 cut(s) 60, 115, 471, 885
TaaI ACNGT 4 cut(s) 41, 384, 704, 934
TaqI TCGA 4 cut(s) 222, 243, 318, 737
TasI AATT 7 cut(s) 7, 88, 347, 460, 833, 875, 924
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 3 cut(s) 224, 403, 920
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 3 cut(s) 356, 869, 923
TspRI CASTG 1 cut(s) 360
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 3 cut(s) 455, 468, 505
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 5 cut(s) 7, 347, 460, 833, 875
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.