pycom10g27800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013988
Physical Location & Seq
Forward (+)
28686066 .. 28687934
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27800.1

Sequence Viewer

Length: 1095 bp
ATGTATTATATAAAAAAATCCAACTTATTTACGGATCTGCCACTCTTTAGAGCCTATAAATTGCACCATAGCTATAGGTACTCCACTCAACAAAAACAAAGGCTACACACAGAGCATACAAACCAAGCAATCCAAAGAGATATGGCGACCTTCCCAGTTGTTGACTTGAGCCTTGTCAATGGTGAAGAGAGAGTAGCAACCTTGGAGAAGATTAATGATGCTTGTGAGAACTGGGGTTTCTTTGAGTTGGTGAACCATGGGATATCTACTGAGCTTTTGGACACTGTGGAGAAGATGACCAAGGATCACTACAAGAAGACCATGGAGCAAAGGTTTAAGGAAATGGTGGCAGCCAAAGGCCTCGACGCTGTCCAGTCCGAAATCCACGACTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTTCCTTCCTCAAACATTTCCGAAGTCCCCGATCTCGAGGAAGATTACAGGAAGACCATGAAAGAATTTGCAGTGGAACTGGAGAAGCTAGCTGAGAAGCTTTTGGACTTGCTGTGTGAGAACCTTGGACTCGAGAAGGGTTATCTGAAGAAGGTTTTCTATGGATCGATCAGTTTCAGGGGTCCGAATTTTGGGACCAAGGTCAGCAACTACCCTCCATGCCCCAAGCCAGACCTGATCAAGGGACTCCGGGCCCACAGCGACGCCGGTGGCATCATCCTGCTTTTCCAGGATGACAAGGTCAGCGGCCTCCAGCTTCTCAAGGATGGTGATTGGGTGGATGTCCCCCCAATGCACCACTCCATTGTCATAAACTTAGGTGACCAGATTGAGGTGATCACCAATGGGAAGTACAAGAGTGTGATGCACCGGGTGATAGCTCAGTCGGACGGGACCAGAATGTCGATAGCCTCGTTCTACAACCCAGGCGATGATGCATTTATCAGCCCAGCACCGGCAGTGCTTGAGAAGAAAACTGAGGACGCCCCAACTTATCCCAAGTTTGTGTTTGATGACTACATGAAGCTGTATTCTGGCCTGAAATTCCAAGCCAAGGAGCCAAGATTTGAAGCTATGAAGGCCAAGGAATCCACCCTTGTTGCAACTACCTGA

Protein Analysis

365

Amino Acids

41.59

Weight (kDa)

5.98

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 52 - 144 5e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 303 9.7e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12010 AT1G62380
fragaria_vesca FvH4_3g01280
malus_domestica MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica Prupe.4G013800_v2.0.a1
pyrus_communis pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis RchiOBHm_Chr5g0001831
rosa_laevigata RLG00000030975
rosa_multiflora Rmu_ssc0000237.1_g000010
rosa_roxburghii Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa Rorug04G0393500
rosa_samantha Rh5DG016100
rosa_wichuraiana Rw5G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 883
AccB7I CCANNNNNTGG 1 cut(s) 391
AciI CCGC 1 cut(s) 729
AclWI GGATC 3 cut(s) 42, 312, 595
AcsI RAATTY 3 cut(s) 488, 610, 1025
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 2 cut(s) 687, 966
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 2 cut(s) 80, 836
AfiI CCNNNNNNNGG 6 cut(s) 391, 614, 664, 814, 937, 1036
AgsI TTSAA 1 cut(s) 1052
AjnI CCWGG 2 cut(s) 711, 907
AluBI AGCT 9 cut(s) 72, 274, 511, 515, 523, 739, 863, 1009, 1055
AluI AGCT 9 cut(s) 72, 274, 511, 515, 523, 739, 863, 1009, 1055
AlwI GGATC 3 cut(s) 42, 312, 595
Ama87I CYCGRG 2 cut(s) 458, 554
AoxI GGCC 5 cut(s) 358, 675, 730, 1018, 1062
ApaI GGGCCC 1 cut(s) 679
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 3 cut(s) 488, 610, 1025
AseI ATTAAT 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 5 cut(s) 605, 618, 675, 676, 876
AsuC2I CCSGG 2 cut(s) 674, 854
AsuHPI GGTGA 7 cut(s) 194, 262, 764, 814, 815, 829, 868
AsuNHI GCTAGC 1 cut(s) 511
AvaI CYCGRG 2 cut(s) 458, 554
AvaII GGWCC 3 cut(s) 605, 618, 876
BaeGI GKGCMC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 679
BbsI GAAGAC 2 cut(s) 323, 482
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 743
BciT130I CCWGG 2 cut(s) 713, 909
BclI TGATCA 2 cut(s) 660, 819
BcnI CCSGG 2 cut(s) 674, 854
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 73
BisI GCNGC 2 cut(s) 351, 730
BlsI GCNGC 2 cut(s) 352, 731
Bme1390I CCNGG 4 cut(s) 674, 713, 854, 909
Bme18I GGWCC 3 cut(s) 605, 618, 876
BmeT110I CYCGRG 2 cut(s) 458, 554
BmgT120I GGNCC 5 cut(s) 605, 618, 675, 676, 876
BmiI GGNNCC 5 cut(s) 606, 619, 677, 877, 1041
BmrFI CCNGG 4 cut(s) 674, 713, 854, 909
BmrI ACTGGG 3 cut(s) 149, 241, 406
BmsI GCATC 4 cut(s) 208, 705, 837, 907
BmtI GCTAGC 1 cut(s) 515
BmuI ACTGGG 3 cut(s) 149, 241, 406
BoxI GACNNNNGTC 1 cut(s) 623
BpiI GAAGAC 2 cut(s) 323, 482
BpmI CTGGAG 2 cut(s) 524, 719
BpuEI CTTGAG 3 cut(s) 187, 728, 968
BpuMI CCSGG 2 cut(s) 674, 854
Bsa29I ATCGAT 1 cut(s) 590
BsaHI GRCGYC 2 cut(s) 687, 966
BsaJI CCNNGG 9 cut(s) 201, 256, 300, 321, 547, 621, 907, 1035, 1065
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 6 cut(s) 391, 614, 664, 814, 937, 1036
Bse118I RCCGGY 2 cut(s) 689, 937
Bse1I ACTGG 5 cut(s) 155, 236, 373, 401, 507
BseBI CCWGG 2 cut(s) 713, 909
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 9 cut(s) 201, 256, 300, 321, 547, 621, 907, 1035, 1065
BseGI GGATG 4 cut(s) 699, 721, 754, 769
BseLI CCNNNNNNNGG 6 cut(s) 391, 614, 664, 814, 937, 1036
BseMII CTCAG 4 cut(s) 261, 507, 878, 951
BseNI ACTGG 5 cut(s) 155, 236, 373, 401, 507
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 1 cut(s) 362
BseYI CCCAGC 1 cut(s) 931
BshFI GGCC 5 cut(s) 360, 677, 732, 1020, 1064
BshVI ATCGAT 1 cut(s) 590
BsiHKCI CYCGRG 2 cut(s) 458, 554
BsiSI CCGG 4 cut(s) 673, 690, 853, 938
BslFI GGGAC 5 cut(s) 434, 631, 681, 752, 889
BslI CCNNNNNNNGG 6 cut(s) 391, 614, 664, 814, 937, 1036
BsmFI GGGAC 5 cut(s) 434, 631, 681, 752, 889
BsnI GGCC 5 cut(s) 360, 677, 732, 1020, 1064
BsoBI CYCGRG 2 cut(s) 458, 554
Bsp120I GGGCCC 1 cut(s) 675
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
Bsp19I CCATGG 2 cut(s) 256, 321
BspACI CCGC 1 cut(s) 729
BspANI GGCC 5 cut(s) 360, 677, 732, 1020, 1064
BspCNI CTCAG 4 cut(s) 262, 508, 877, 952
BspDI ATCGAT 1 cut(s) 590
BspLI GGNNCC 5 cut(s) 606, 619, 677, 877, 1041
BspOI GCTAGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 42, 312, 595
BsrFI RCCGGY 2 cut(s) 689, 937
BsrI ACTGG 5 cut(s) 155, 236, 373, 401, 507
BssAI RCCGGY 2 cut(s) 689, 937
BssECI CCNNGG 9 cut(s) 201, 256, 300, 321, 547, 621, 907, 1035, 1065
BssMI GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
BssNI GRCGYC 2 cut(s) 687, 966
BssT1I CCWWGG 8 cut(s) 201, 256, 300, 321, 547, 621, 1035, 1065
Bst2UI CCWGG 2 cut(s) 713, 909
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 2 cut(s) 687, 966
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 5 cut(s) 270, 516, 799, 864, 960
BstDSI CCRYGG 2 cut(s) 256, 321
BstEII GGTNACC 1 cut(s) 803
BstENI CCTNNNNNAGG 1 cut(s) 662
BstF5I GGATG 4 cut(s) 699, 721, 754, 769
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 7 cut(s) 37, 307, 457, 590, 594, 663, 822
BstMBI GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
BstMWI GCNNNNNNNGC 1 cut(s) 1061
BstNI CCWGG 2 cut(s) 713, 909
BstPAI GACNNNNGTC 1 cut(s) 623
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 672, 711, 852, 907
BstSFI CTRYAG 1 cut(s) 73
BstSLI GKGCMC 1 cut(s) 679
BstV1I GCAGC 1 cut(s) 362
BstV2I GAAGAC 2 cut(s) 323, 482
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 590
BsuRI GGCC 5 cut(s) 360, 677, 732, 1020, 1064
BsuTUI ATCGAT 1 cut(s) 590
BtgI CCRYGG 2 cut(s) 256, 321
BtgZI GCGATG 1 cut(s) 927
BtsCI GGATG 4 cut(s) 699, 721, 754, 769
BtsI GCAGTG 2 cut(s) 501, 948
BtsIMutI CAGTG 3 cut(s) 282, 501, 948
Cac8I GCNNGC 1 cut(s) 513
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 2 cut(s) 689, 937
Cfr13I GGNCC 5 cut(s) 605, 618, 675, 676, 876
ClaI ATCGAT 1 cut(s) 590
CseI GACGC 3 cut(s) 374, 695, 974
Csp6I GTAC 2 cut(s) 79, 835
CspCI CAANNNNNGTGG 1 cut(s) 1063
CviAII CATG 5 cut(s) 257, 322, 481, 642, 1003
CviQI GTAC 2 cut(s) 79, 835
DdeI CTNAG 5 cut(s) 270, 516, 799, 864, 960
DpnI GATC 7 cut(s) 36, 306, 456, 589, 593, 662, 821
DpnII GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
DraIII CACNNNGTG 1 cut(s) 856
DrdI GACNNNNNNGTC 1 cut(s) 883
DseDI GACNNNNNNGTC 1 cut(s) 883
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 8 cut(s) 201, 256, 300, 321, 547, 621, 1035, 1065
Eco147I AGGCCT 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 679
Eco32I GATATC 1 cut(s) 264
Eco47I GGWCC 3 cut(s) 605, 618, 876
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 2 cut(s) 458, 554
Eco91I GGTNACC 1 cut(s) 803
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 803
EcoRII CCWGG 2 cut(s) 711, 907
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 8 cut(s) 201, 256, 300, 321, 547, 621, 1035, 1065
EcoT22I ATGCAT 1 cut(s) 922
EcoT38I GRGCYC 1 cut(s) 679
ErhI CCWWGG 8 cut(s) 201, 256, 300, 321, 547, 621, 1035, 1065
FaeI CATG 5 cut(s) 260, 325, 484, 645, 1006
FaqI GGGAC 5 cut(s) 434, 631, 681, 752, 889
FatI CATG 5 cut(s) 256, 321, 480, 641, 1002
FbaI TGATCA 2 cut(s) 660, 819
Fnu4HI GCNGC 2 cut(s) 351, 730
FokI GGATG 4 cut(s) 686, 728, 761, 776
FriOI GRGCYC 1 cut(s) 679
Fsp4HI GCNGC 2 cut(s) 351, 730
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 419
GluI GCNGC 2 cut(s) 351, 730
GsaI CCCAGC 1 cut(s) 935
GsuI CTGGAG 2 cut(s) 524, 719
HaeIII GGCC 5 cut(s) 360, 677, 732, 1020, 1064
HapII CCGG 4 cut(s) 673, 690, 853, 938
HgaI GACGC 3 cut(s) 374, 695, 974
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 2 cut(s) 687, 966
Hin1II CATG 5 cut(s) 260, 325, 484, 645, 1006
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 552, 669, 1070
HpaII CCGG 4 cut(s) 673, 690, 853, 938
HphI GGTGA 7 cut(s) 194, 262, 764, 814, 815, 829, 868
Hpy166II GTNNAC 2 cut(s) 163, 253
Hpy188I TCNGA 5 cut(s) 379, 445, 570, 609, 871
Hpy188III TCNNGA 2 cut(s) 458, 556
Hpy8I GTNNAC 2 cut(s) 163, 253
Hpy99I CGWCG 2 cut(s) 368, 689
HpyAV CCTTC 7 cut(s) 160, 418, 434, 438, 553, 568, 1054
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 6 cut(s) 64, 494, 778, 850, 920, 1085
HpyF10VI GCNNNNNNNGC 1 cut(s) 1061
HpyF3I CTNAG 5 cut(s) 270, 516, 799, 864, 960
Hsp92I GRCGYC 2 cut(s) 687, 966
Hsp92II CATG 5 cut(s) 260, 325, 484, 645, 1006
HspAI GCGC 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 660, 819
Kzo9I GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
LmnI GCTCC 2 cut(s) 325, 1039
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 4 cut(s) 208, 705, 837, 907
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 1 cut(s) 803
MalI GATC 7 cut(s) 36, 306, 456, 589, 593, 662, 821
MboI GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
MboII GAAGA 9 cut(s) 197, 220, 304, 328, 403, 476, 487, 583, 964
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 4 cut(s) 59, 488, 610, 1025
MlyI GAGTC 2 cut(s) 546, 663
MmeI TCCRAC 2 cut(s) 45, 849
MnlI CCTC 8 cut(s) 371, 442, 454, 648, 743, 808, 904, 955
Mph1103I ATGCAT 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 2 cut(s) 213, 336
MspA1I CMGCKG 1 cut(s) 729
MspI CCGG 4 cut(s) 673, 690, 853, 938
MspR9I CCNGG 4 cut(s) 674, 713, 854, 909
MvaI CCWGG 2 cut(s) 713, 909
MwoI GCNNNNNNNGC 1 cut(s) 1061
NciI CCSGG 2 cut(s) 674, 854
NcoI CCATGG 2 cut(s) 256, 321
NdeII GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
NheI GCTAGC 1 cut(s) 511
NlaIII CATG 5 cut(s) 260, 325, 484, 645, 1006
NlaIV GGNNCC 5 cut(s) 606, 619, 677, 877, 1041
NmuCI GTSAC 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 922
PaeR7I CTCGAG 2 cut(s) 458, 554
PceI AGGCCT 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 893
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 1070
PflFI GACNNNGTC 2 cut(s) 368, 722
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 711
PkrI GCNGC 2 cut(s) 352, 731
PleI GAGTC 2 cut(s) 546, 663
PpsI GAGTC 2 cut(s) 546, 663
PshAI GACNNNNGTC 1 cut(s) 623
PshBI ATTAAT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 711, 907
PspEI GGTNACC 1 cut(s) 803
PspFI CCCAGC 1 cut(s) 931
PspGI CCWGG 2 cut(s) 711, 907
PspN4I GGNNCC 5 cut(s) 606, 619, 677, 877, 1041
PspOMI GGGCCC 1 cut(s) 675
PspPI GGNCC 5 cut(s) 605, 618, 675, 676, 876
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 2 cut(s) 368, 722
RsaI GTAC 2 cut(s) 80, 836
RsaNI GTAC 2 cut(s) 79, 835
SaqAI TTAA 2 cut(s) 213, 336
SatI GCNGC 2 cut(s) 351, 730
Sau3AI GATC 7 cut(s) 34, 304, 454, 587, 591, 660, 819
Sau96I GGNCC 5 cut(s) 605, 618, 675, 676, 876
SchI GAGTC 2 cut(s) 546, 663
ScrFI CCNGG 4 cut(s) 674, 713, 854, 909
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 4 cut(s) 208, 705, 837, 907
SfcI CTRYAG 1 cut(s) 73
Sfr274I CTCGAG 2 cut(s) 458, 554
SgrAI CRCCGGYG 1 cut(s) 689
SinI GGWCC 3 cut(s) 605, 618, 876
SlaI CTCGAG 2 cut(s) 458, 554
SmlI CTYRAG 5 cut(s) 166, 458, 554, 743, 947
SmoI CTYRAG 5 cut(s) 166, 458, 554, 743, 947
Sse9I AATT 4 cut(s) 59, 488, 610, 1025
SseBI AGGCCT 1 cut(s) 360
SsiI CCGC 1 cut(s) 729
SspMI CTAG 1 cut(s) 512
StuI AGGCCT 1 cut(s) 360
StyD4I CCNGG 4 cut(s) 672, 711, 852, 907
StyI CCWWGG 8 cut(s) 201, 256, 300, 321, 547, 621, 1035, 1065
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 5 cut(s) 363, 459, 555, 590, 887
TasI AATT 4 cut(s) 59, 488, 610, 1025
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 1 cut(s) 1070
Tru1I TTAA 2 cut(s) 213, 336
Tru9I TTAA 2 cut(s) 213, 336
TscAI CASTG 3 cut(s) 289, 501, 948
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 3 cut(s) 497, 1019, 1073
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 289, 501, 948
Tth111I GACNNNGTC 2 cut(s) 368, 722
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 3 cut(s) 605, 618, 876
VspI ATTAAT 1 cut(s) 213
XagI CCTNNNNNAGG 1 cut(s) 662
XapI RAATTY 3 cut(s) 488, 610, 1025
XhoI CTCGAG 2 cut(s) 458, 554
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.