MD10G1328100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013988
Physical Location & Seq
Forward (+)
40754906 .. 40756831
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1328100.v1.1.491

Sequence Viewer

Length: 945 bp
ATGGCGACTTTCCCAGTTGTTGACTTGAGCCTTGTCAATGGTGAAGAGAGAGCAGCAACCTTGGAGAAGATCAATGATGCTTGTGAGAACTGGGGTTTCTTTGAGCTGGTGAACCATGGGATGTCTACTGAGCTTTTGGACACTGTGGAGAAGATGACCAAGGATCACTACAAGAAGACCATGGAGCAAAGGTTTAAGGAAATGGTGGCAGCCAAAGGCCTCGACGATGTCCAGTCCGAAATCCACGACTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTTCCTTCCTCAAACATTTCCGAAATCCCTGATCTCGAGGAAGAGTACAGGAAGACCATGAAGGAATTTGCAGTGGAACTGGAGAAGCTAGCTGAGAAGCTTTTGGACTTGCTGTGTGAGAATCTTGGACTCGAGAAGGGTTATCTGAAGAAGGTTTTCTATGGATCCAAGGGTCCGAATTTTGGGACCAAGGTCAGCAACTACCCTCCATGCCCCAAGCCAGACCTGATCAAGGGACTCCGGGCCCACAGCGACGCCGGTGGCATCATCCTGCTTTTCCAGGATGACAAGGTCAGCGGCCTCCAGCTTCTCAAGGATGGTGAATGGGTGGATGTCCCCCCAATGCACCACTCCATTGTCATAAACTTAGGTGACCAGATTGAGGTGATCACCAATGGGAAGTACAAAAGTGTGATGCACCGGGTGATAGCTCAGTCGGATGGGACCAGAATGTCGATAGCCTCGTTCTACAACCCAGGCAACGACTCATTCATCAGCCCAGCACCGGCAGTGCTTGAGAAGAAAACTGAGGACGCCCCAACTTATCCCAAGTTTGTGTTTGATGACTACATGAAGCTGTATTCTGGCCTGAAATTCCAAGCCAAGGAGCCAAGATTTGAAGCTATGAAGGCCAAGGAATCCACCCCTGTTGCAACTGCCTGA

Protein Analysis

315

Amino Acids

35.41

Weight (kDa)

5.24

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 5 - 96 3.7e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 159 - 253 6.3e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12010 AT1G62380
fragaria_vesca FvH4_3g01280
malus_domestica MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica Prupe.4G013800_v2.0.a1
pyrus_communis pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis RchiOBHm_Chr5g0001831
rosa_laevigata RLG00000030975
rosa_multiflora Rmu_ssc0000237.1_g000010
rosa_roxburghii Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa Rorug04G0393500
rosa_samantha Rh5DG016100
rosa_wichuraiana Rw5G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 733
AccB7I CCANNNNNTGG 1 cut(s) 250
AccI GTMKAC 1 cut(s) 125
AciI CCGC 1 cut(s) 579
AclWI GGATC 3 cut(s) 171, 441, 454
AcsI RAATTY 3 cut(s) 347, 460, 875
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 2 cut(s) 537, 816
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 2 cut(s) 329, 686
AfiI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
AgsI TTSAA 1 cut(s) 902
AjnI CCWGG 2 cut(s) 561, 757
AluBI AGCT 9 cut(s) 106, 133, 370, 374, 382, 589, 713, 859, 905
AluI AGCT 9 cut(s) 106, 133, 370, 374, 382, 589, 713, 859, 905
AlwI GGATC 3 cut(s) 171, 441, 454
Ama87I CYCGRG 2 cut(s) 317, 413
AoxI GGCC 5 cut(s) 217, 525, 580, 868, 912
ApaI GGGCCC 1 cut(s) 529
ApeKI GCWGC 2 cut(s) 53, 209
ApoI RAATTY 3 cut(s) 347, 460, 875
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 5 cut(s) 455, 468, 525, 526, 726
AsuC2I CCSGG 2 cut(s) 524, 704
AsuHPI GGTGA 7 cut(s) 53, 121, 614, 664, 665, 679, 718
AsuNHI GCTAGC 1 cut(s) 370
AvaI CYCGRG 2 cut(s) 317, 413
AvaII GGWCC 3 cut(s) 455, 468, 726
BaeGI GKGCMC 1 cut(s) 529
BamHI GGATCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 529
BbsI GAAGAC 2 cut(s) 182, 341
BbvI GCAGC 2 cut(s) 65, 221
BccI CCATC 2 cut(s) 593, 716
BciT130I CCWGG 2 cut(s) 563, 759
BclI TGATCA 2 cut(s) 510, 669
BcnI CCSGG 2 cut(s) 524, 704
BfaI CTAG 1 cut(s) 371
BisI GCNGC 3 cut(s) 54, 210, 580
BlsI GCNGC 3 cut(s) 55, 211, 581
Bme1390I CCNGG 4 cut(s) 524, 563, 704, 759
Bme18I GGWCC 3 cut(s) 455, 468, 726
BmeT110I CYCGRG 2 cut(s) 317, 413
BmgT120I GGNCC 5 cut(s) 455, 468, 525, 526, 726
BmiI GGNNCC 6 cut(s) 448, 456, 469, 527, 727, 891
BmrFI CCNGG 4 cut(s) 524, 563, 704, 759
BmrI ACTGGG 3 cut(s) 8, 100, 265
BmsI GCATC 3 cut(s) 67, 555, 687
BmtI GCTAGC 1 cut(s) 374
BmuI ACTGGG 3 cut(s) 8, 100, 265
BoxI GACNNNNGTC 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 182, 341
BpmI CTGGAG 2 cut(s) 383, 569
BpuEI CTTGAG 3 cut(s) 46, 578, 818
BpuMI CCSGG 2 cut(s) 524, 704
BsaHI GRCGYC 2 cut(s) 537, 816
BsaJI CCNNGG 9 cut(s) 60, 115, 159, 180, 450, 471, 757, 885, 915
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
Bse118I RCCGGY 2 cut(s) 539, 787
Bse1I ACTGG 5 cut(s) 14, 95, 232, 260, 366
BseBI CCWGG 2 cut(s) 563, 759
BseDI CCNNGG 9 cut(s) 60, 115, 159, 180, 450, 471, 757, 885, 915
BseGI GGATG 6 cut(s) 126, 549, 571, 604, 619, 727
BseLI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
BseMII CTCAG 4 cut(s) 120, 366, 728, 801
BseNI ACTGG 5 cut(s) 14, 95, 232, 260, 366
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 2 cut(s) 65, 221
BseYI CCCAGC 1 cut(s) 781
BshFI GGCC 5 cut(s) 219, 527, 582, 870, 914
BsiHKCI CYCGRG 2 cut(s) 317, 413
BsiSI CCGG 4 cut(s) 523, 540, 703, 788
BslFI GGGAC 4 cut(s) 481, 531, 602, 739
BslI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
BsmFI GGGAC 4 cut(s) 481, 531, 602, 739
BsnI GGCC 5 cut(s) 219, 527, 582, 870, 914
BsoBI CYCGRG 2 cut(s) 317, 413
Bsp120I GGGCCC 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 6 cut(s) 69, 163, 313, 446, 510, 669
Bsp19I CCATGG 2 cut(s) 115, 180
BspACI CCGC 1 cut(s) 579
BspANI GGCC 5 cut(s) 219, 527, 582, 870, 914
BspCNI CTCAG 4 cut(s) 121, 367, 727, 802
BspLI GGNNCC 6 cut(s) 448, 456, 469, 527, 727, 891
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 3 cut(s) 171, 441, 454
BsrFI RCCGGY 2 cut(s) 539, 787
BsrI ACTGG 5 cut(s) 14, 95, 232, 260, 366
BssAI RCCGGY 2 cut(s) 539, 787
BssECI CCNNGG 9 cut(s) 60, 115, 159, 180, 450, 471, 757, 885, 915
BssMI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
BssNI GRCGYC 2 cut(s) 537, 816
BssT1I CCWWGG 8 cut(s) 60, 115, 159, 180, 450, 471, 885, 915
Bst2UI CCWGG 2 cut(s) 563, 759
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 39, 318
BstACI GRCGYC 2 cut(s) 537, 816
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 5 cut(s) 129, 375, 649, 714, 810
BstDSI CCRYGG 2 cut(s) 115, 180
BstEII GGTNACC 1 cut(s) 653
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 6 cut(s) 126, 549, 571, 604, 619, 727
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 6 cut(s) 72, 166, 316, 449, 513, 672
BstMBI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
BstMWI GCNNNNNNNGC 1 cut(s) 911
BstNI CCWGG 2 cut(s) 563, 759
BstPAI GACNNNNGTC 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 4 cut(s) 522, 561, 702, 757
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 2 cut(s) 65, 221
BstV2I GAAGAC 2 cut(s) 182, 341
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 5 cut(s) 219, 527, 582, 870, 914
BtgI CCRYGG 2 cut(s) 115, 180
BtsCI GGATG 6 cut(s) 126, 549, 571, 604, 619, 727
BtsI GCAGTG 2 cut(s) 360, 798
BtsIMutI CAGTG 3 cut(s) 141, 360, 798
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 2 cut(s) 539, 787
Cfr13I GGNCC 5 cut(s) 455, 468, 525, 526, 726
CseI GACGC 2 cut(s) 545, 824
Csp6I GTAC 2 cut(s) 328, 685
CspCI CAANNNNNGTGG 1 cut(s) 913
CviAII CATG 5 cut(s) 116, 181, 340, 492, 853
CviQI GTAC 2 cut(s) 328, 685
DdeI CTNAG 5 cut(s) 129, 375, 649, 714, 810
DpnI GATC 6 cut(s) 71, 165, 315, 448, 512, 671
DpnII GATC 6 cut(s) 69, 163, 313, 446, 510, 669
DraIII CACNNNGTG 1 cut(s) 706
DrdI GACNNNNNNGTC 1 cut(s) 733
DseDI GACNNNNNNGTC 1 cut(s) 733
Eam1104I CTCTTC 2 cut(s) 39, 318
EarI CTCTTC 2 cut(s) 39, 318
Eco130I CCWWGG 8 cut(s) 60, 115, 159, 180, 450, 471, 885, 915
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 529
Eco47I GGWCC 3 cut(s) 455, 468, 726
Eco57I CTGAAG 1 cut(s) 449
Eco88I CYCGRG 2 cut(s) 317, 413
Eco91I GGTNACC 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 561, 757
EcoT14I CCWWGG 8 cut(s) 60, 115, 159, 180, 450, 471, 885, 915
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 8 cut(s) 60, 115, 159, 180, 450, 471, 885, 915
FaeI CATG 5 cut(s) 119, 184, 343, 495, 856
FaiI YATR 8 cut(s) 117, 182, 341, 444, 493, 644, 854, 908
FaqI GGGAC 4 cut(s) 481, 531, 602, 739
FatI CATG 5 cut(s) 115, 180, 339, 491, 852
FbaI TGATCA 2 cut(s) 510, 669
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 54, 210, 580
FokI GGATG 6 cut(s) 133, 536, 578, 611, 626, 734
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 3 cut(s) 54, 210, 580
FspBI CTAG 1 cut(s) 371
GlaI GCGC 1 cut(s) 278
GluI GCNGC 3 cut(s) 54, 210, 580
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 2 cut(s) 383, 569
HaeIII GGCC 5 cut(s) 219, 527, 582, 870, 914
HapII CCGG 4 cut(s) 523, 540, 703, 788
HgaI GACGC 2 cut(s) 545, 824
HhaI GCGC 1 cut(s) 279
Hin1I GRCGYC 2 cut(s) 537, 816
Hin1II CATG 5 cut(s) 119, 184, 343, 495, 856
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 5 cut(s) 403, 411, 519, 767, 920
HpaII CCGG 4 cut(s) 523, 540, 703, 788
HphI GGTGA 7 cut(s) 53, 121, 614, 664, 665, 679, 718
Hpy166II GTNNAC 3 cut(s) 22, 112, 126
Hpy188I TCNGA 5 cut(s) 238, 304, 429, 459, 721
Hpy188III TCNNGA 2 cut(s) 317, 415
Hpy8I GTNNAC 3 cut(s) 22, 112, 126
Hpy99I CGWCG 2 cut(s) 227, 539
HpyAV CCTTC 7 cut(s) 277, 293, 297, 337, 412, 427, 904
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 4 cut(s) 353, 628, 700, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 911
HpyF3I CTNAG 5 cut(s) 129, 375, 649, 714, 810
Hsp92I GRCGYC 2 cut(s) 537, 816
Hsp92II CATG 5 cut(s) 119, 184, 343, 495, 856
HspAI GCGC 1 cut(s) 277
Ksp22I TGATCA 2 cut(s) 510, 669
Kzo9I GATC 6 cut(s) 69, 163, 313, 446, 510, 669
LmnI GCTCC 2 cut(s) 184, 889
Lsp1109I GCAGC 2 cut(s) 65, 221
LweI GCATC 3 cut(s) 67, 555, 687
MaeI CTAG 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 653
MalI GATC 6 cut(s) 71, 165, 315, 448, 512, 671
MboI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
MboII GAAGA 9 cut(s) 56, 79, 163, 187, 262, 335, 346, 442, 814
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 3 cut(s) 347, 460, 875
MlyI GAGTC 3 cut(s) 405, 513, 761
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 8 cut(s) 230, 301, 313, 498, 593, 658, 754, 805
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 579
MspI CCGG 4 cut(s) 523, 540, 703, 788
MspR9I CCNGG 4 cut(s) 524, 563, 704, 759
MvaI CCWGG 2 cut(s) 563, 759
MwoI GCNNNNNNNGC 1 cut(s) 911
NciI CCSGG 2 cut(s) 524, 704
NcoI CCATGG 2 cut(s) 115, 180
NdeII GATC 6 cut(s) 69, 163, 313, 446, 510, 669
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 5 cut(s) 119, 184, 343, 495, 856
NlaIV GGNNCC 6 cut(s) 448, 456, 469, 527, 727, 891
NmuCI GTSAC 1 cut(s) 653
PaeR7I CTCGAG 2 cut(s) 317, 413
PceI AGGCCT 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 743
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 403, 920
PflFI GACNNNGTC 2 cut(s) 227, 572
PflMI CCANNNNNTGG 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 3 cut(s) 55, 211, 581
PleI GAGTC 3 cut(s) 405, 513, 761
PpsI GAGTC 3 cut(s) 405, 513, 761
PshAI GACNNNNGTC 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 561, 757
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 2 cut(s) 561, 757
PspN4I GGNNCC 6 cut(s) 448, 456, 469, 527, 727, 891
PspOMI GGGCCC 1 cut(s) 525
PspPI GGNCC 5 cut(s) 455, 468, 525, 526, 726
PsuI RGATCY 1 cut(s) 446
PsyI GACNNNGTC 2 cut(s) 227, 572
RsaI GTAC 2 cut(s) 329, 686
RsaNI GTAC 2 cut(s) 328, 685
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 54, 210, 580
Sau3AI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
Sau96I GGNCC 5 cut(s) 455, 468, 525, 526, 726
SchI GAGTC 3 cut(s) 405, 513, 761
ScrFI CCNGG 4 cut(s) 524, 563, 704, 759
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 3 cut(s) 67, 555, 687
Sfr274I CTCGAG 2 cut(s) 317, 413
SgrAI CRCCGGYG 1 cut(s) 539
SinI GGWCC 3 cut(s) 455, 468, 726
SlaI CTCGAG 2 cut(s) 317, 413
SmlI CTYRAG 5 cut(s) 25, 317, 413, 593, 797
SmoI CTYRAG 5 cut(s) 25, 317, 413, 593, 797
Sse9I AATT 3 cut(s) 347, 460, 875
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 1 cut(s) 579
SspMI CTAG 1 cut(s) 371
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 4 cut(s) 522, 561, 702, 757
StyI CCWWGG 8 cut(s) 60, 115, 159, 180, 450, 471, 885, 915
TaaI ACNGT 1 cut(s) 145
TaqI TCGA 4 cut(s) 222, 318, 414, 737
TasI AATT 3 cut(s) 347, 460, 875
TatI WGTACW 2 cut(s) 327, 684
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 2 cut(s) 403, 920
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 3 cut(s) 148, 360, 798
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 53, 209
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 4 cut(s) 356, 763, 869, 923
TspRI CASTG 3 cut(s) 148, 360, 798
Tth111I GACNNNGTC 2 cut(s) 227, 572
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 3 cut(s) 455, 468, 726
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 3 cut(s) 347, 460, 875
XhoI CTCGAG 2 cut(s) 317, 413
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.