pycom05g32140

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013988
Physical Location & Seq
Forward (+)
31669847 .. 31671384
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32140.3

Sequence Viewer

Length: 945 bp
ATGGCGACTTTCCCAGTTGTTGACATGAGCCTTGTCAATGGTGAAGAGAGAGCAGCAACCTTGGAGAAGATCAATGATGCATGTGAGAACTGGGGTTTCTTTGAGTTGGTGAACCATGGGATATCAACTGAGCTTTTGGACACTGTGGAGAAGATGAACAAGGATCACTACAAGAAGACCATGGAGCAAAGGTTCAAGGAAATGGTGGCAGCCAAAGGCCTCGACGCTGTCCAGTCCGAAATCCACGACTTGGACTGGGAAAGCACCTTCTTCTTGCGCCACCTTCCTTCCTCAAACATTTCCGAAATCCCTGATCTCGAGGAAGATTACAGGAAGACCATGAAGGAATTTGCAGTGGAATTGGAGAAGCTAGCTGAGAAGCTTTTGGAGTTGCTGTGTGAGAATCTTGGACTTGAGAAGGGCTATCTAAAGAAGGCTTTCTATGGATCCAAGGGTCCGAATTTTGGGACCAAGGTCAGCAACTACCCTCCATGTCCCAAGCCAGACCTGATCAAGGGACTCCGGGCCCACACCGACGCTGGTGGTATCATCCTGCTCTTCCAGGATGACAAGGTCAGTGGCCTCCAGCTCCTCAAGGATGGTGAATGGGTGGATGTCCCCCCAAGGCACCACTCCATTGTCATCAACTTAGGTGACCAGATTGAGGTGATCACCAATGGGAAGTACAAGAGTATAATGCACCGGGTGATAGCTCAGTCGGACGGAACCAGAATGTCGATAGCCTCGTTCTACAACCCGTGCGACGATGCGTTTATCAGCCCGGCACCGGCATTGCTGGAGAACAAATCTGAGGAAACCCCAACTTATCCCAAATTTTTGTTTGATGATTACATGAAGCTGTATTCTGGCCTCAAATTCCAAGCCAAGGAGCCAAGATTTGAAGCTATGAAGGCCAGGGAAACCACCTCGGTTGCAACTGCCTGA

Protein Analysis

315

Amino Acids

35.51

Weight (kDa)

5.25

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12010 AT1G62380
fragaria_vesca FvH4_3g01280
malus_domestica MD05G1354000.v1.1 MD10G1328100.v1.1
prunus_persica Prupe.4G013800_v2.0.a1
pyrus_communis pycom05g32140 pycom10g27800 pycom17g07320
rosa_chinensis RchiOBHm_Chr5g0001831
rosa_laevigata RLG00000030975
rosa_multiflora Rmu_ssc0000237.1_g000010
rosa_roxburghii Rroxscaffold_1G00071260 Rroxscaffold_1G00074520
rosa_rugosa Rorug04G0393500
rosa_samantha Rh5DG016100
rosa_wichuraiana Rw5G001410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 627, 784
AccB7I CCANNNNNTGG 1 cut(s) 250
AclWI GGATC 3 cut(s) 171, 441, 454
AcsI RAATTY 4 cut(s) 347, 460, 833, 875
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
AgsI TTSAA 2 cut(s) 196, 902
AjnI CCWGG 2 cut(s) 561, 914
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 8 cut(s) 133, 370, 374, 382, 589, 713, 859, 905
AluI AGCT 8 cut(s) 133, 370, 374, 382, 589, 713, 859, 905
AlwI GGATC 3 cut(s) 171, 441, 454
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 5 cut(s) 217, 525, 580, 868, 912
ApaI GGGCCC 1 cut(s) 529
ApeKI GCWGC 2 cut(s) 53, 209
ApoI RAATTY 4 cut(s) 347, 460, 833, 875
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 4 cut(s) 455, 468, 525, 526
AsuC2I CCSGG 3 cut(s) 524, 704, 782
AsuHPI GGTGA 7 cut(s) 53, 121, 614, 664, 665, 679, 718
AsuNHI GCTAGC 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 455, 468
BaeGI GKGCMC 1 cut(s) 529
BamHI GGATCC 1 cut(s) 446
BanI GGYRCC 2 cut(s) 627, 784
BanII GRGCYC 1 cut(s) 529
BbsI GAAGAC 2 cut(s) 182, 341
BbvI GCAGC 2 cut(s) 65, 221
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 2 cut(s) 563, 916
BclI TGATCA 2 cut(s) 510, 669
BcnI CCSGG 3 cut(s) 524, 704, 782
BfaI CTAG 1 cut(s) 371
BisI GCNGC 2 cut(s) 54, 210
BlsI GCNGC 2 cut(s) 55, 211
Bme1390I CCNGG 5 cut(s) 524, 563, 704, 782, 916
Bme18I GGWCC 2 cut(s) 455, 468
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 4 cut(s) 455, 468, 525, 526
BmiI GGNNCC 8 cut(s) 448, 456, 469, 527, 629, 727, 786, 891
BmrFI CCNGG 5 cut(s) 524, 563, 704, 782, 916
BmrI ACTGGG 3 cut(s) 8, 100, 265
BmsI GCATC 2 cut(s) 67, 757
BmtI GCTAGC 1 cut(s) 374
BmuI ACTGGG 3 cut(s) 8, 100, 265
BoxI GACNNNNGTC 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 182, 341
BpmI CTGGAG 2 cut(s) 569, 818
BpuEI CTTGAG 2 cut(s) 434, 578
BpuMI CCSGG 3 cut(s) 524, 704, 782
BsaJI CCNNGG 9 cut(s) 60, 115, 180, 450, 471, 623, 885, 915, 927
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 380, 410
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
Bse118I RCCGGY 1 cut(s) 787
Bse1I ACTGG 4 cut(s) 14, 95, 232, 260
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 2 cut(s) 563, 916
BseDI CCNNGG 9 cut(s) 60, 115, 180, 450, 471, 623, 885, 915, 927
BseGI GGATG 4 cut(s) 549, 571, 604, 619
BseLI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
BseMI GCAATG 1 cut(s) 791
BseMII CTCAG 4 cut(s) 120, 366, 728, 801
BseNI ACTGG 4 cut(s) 14, 95, 232, 260
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 2 cut(s) 65, 221
BshFI GGCC 5 cut(s) 219, 527, 582, 870, 914
BshNI GGYRCC 2 cut(s) 627, 784
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 4 cut(s) 523, 703, 782, 788
BslFI GGGAC 4 cut(s) 480, 481, 531, 602
BslI CCNNNNNNNGG 6 cut(s) 250, 464, 514, 664, 787, 886
BsmFI GGGAC 4 cut(s) 480, 481, 531, 602
BsnI GGCC 5 cut(s) 219, 527, 582, 870, 914
BsoBI CYCGRG 1 cut(s) 317
Bsp120I GGGCCC 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 6 cut(s) 69, 163, 313, 446, 510, 669
Bsp19I CCATGG 2 cut(s) 115, 180
BspANI GGCC 5 cut(s) 219, 527, 582, 870, 914
BspCNI CTCAG 4 cut(s) 121, 367, 727, 802
BspLI GGNNCC 8 cut(s) 448, 456, 469, 527, 629, 727, 786, 891
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 3 cut(s) 171, 441, 454
BspQI GCTCTTC 1 cut(s) 563
BspT107I GGYRCC 2 cut(s) 627, 784
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 787
BsrI ACTGG 4 cut(s) 14, 95, 232, 260
BssAI RCCGGY 1 cut(s) 787
BssECI CCNNGG 9 cut(s) 60, 115, 180, 450, 471, 623, 885, 915, 927
BssMI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
BssT1I CCWWGG 7 cut(s) 60, 115, 180, 450, 471, 623, 885
Bst2UI CCWGG 2 cut(s) 563, 916
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 39, 563
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 5 cut(s) 129, 375, 649, 714, 810
BstDSI CCRYGG 2 cut(s) 115, 180
BstEII GGTNACC 1 cut(s) 653
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 4 cut(s) 549, 571, 604, 619
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 6 cut(s) 72, 166, 316, 449, 513, 672
BstMBI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
BstMWI GCNNNNNNNGC 1 cut(s) 911
BstNI CCWGG 2 cut(s) 563, 916
BstNSI RCATGY 1 cut(s) 84
BstPAI GACNNNNGTC 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 5 cut(s) 522, 561, 702, 780, 914
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 2 cut(s) 65, 221
BstV2I GAAGAC 2 cut(s) 182, 341
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuRI GGCC 5 cut(s) 219, 527, 582, 870, 914
BtgI CCRYGG 2 cut(s) 115, 180
BtsCI GGATG 4 cut(s) 549, 571, 604, 619
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 3 cut(s) 141, 360, 583
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 787
Cfr13I GGNCC 4 cut(s) 455, 468, 525, 526
CseI GACGC 2 cut(s) 233, 545
Csp6I GTAC 1 cut(s) 685
CspCI CAANNNNNGTGG 3 cut(s) 559, 594, 913
CviAII CATG 7 cut(s) 25, 81, 116, 181, 340, 492, 853
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 5 cut(s) 129, 375, 649, 714, 810
DpnI GATC 6 cut(s) 71, 165, 315, 448, 512, 671
DpnII GATC 6 cut(s) 69, 163, 313, 446, 510, 669
DraIII CACNNNGTG 1 cut(s) 706
Eam1104I CTCTTC 2 cut(s) 39, 563
EarI CTCTTC 2 cut(s) 39, 563
Eco130I CCWWGG 7 cut(s) 60, 115, 180, 450, 471, 623, 885
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 529
Eco32I GATATC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 455, 468
Eco88I CYCGRG 1 cut(s) 317
Eco91I GGTNACC 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 561, 914
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 7 cut(s) 60, 115, 180, 450, 471, 623, 885
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 7 cut(s) 60, 115, 180, 450, 471, 623, 885
FaeI CATG 7 cut(s) 28, 84, 119, 184, 343, 495, 856
FaqI GGGAC 4 cut(s) 480, 481, 531, 602
FatI CATG 7 cut(s) 24, 80, 115, 180, 339, 491, 852
FbaI TGATCA 2 cut(s) 510, 669
Fnu4HI GCNGC 2 cut(s) 54, 210
FokI GGATG 4 cut(s) 536, 578, 611, 626
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 2 cut(s) 54, 210
FspBI CTAG 1 cut(s) 371
GlaI GCGC 1 cut(s) 278
GluI GCNGC 2 cut(s) 54, 210
GsuI CTGGAG 2 cut(s) 569, 818
HaeIII GGCC 5 cut(s) 219, 527, 582, 870, 914
HapII CCGG 4 cut(s) 523, 703, 782, 788
HgaI GACGC 2 cut(s) 233, 545
HhaI GCGC 1 cut(s) 279
Hin1II CATG 7 cut(s) 28, 84, 119, 184, 343, 495, 856
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 2 cut(s) 403, 519
HpaII CCGG 4 cut(s) 523, 703, 782, 788
HphI GGTGA 7 cut(s) 53, 121, 614, 664, 665, 679, 718
Hpy166II GTNNAC 2 cut(s) 22, 112
Hpy188I TCNGA 5 cut(s) 238, 304, 459, 721, 811
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 2 cut(s) 22, 112
Hpy99I CGWCG 3 cut(s) 227, 539, 767
HpyAV CCTTC 7 cut(s) 277, 293, 297, 337, 412, 427, 904
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 4 cut(s) 80, 353, 700, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 911
HpyF3I CTNAG 5 cut(s) 129, 375, 649, 714, 810
Hsp92II CATG 7 cut(s) 28, 84, 119, 184, 343, 495, 856
HspAI GCGC 1 cut(s) 277
Ksp22I TGATCA 2 cut(s) 510, 669
Kzo9I GATC 6 cut(s) 69, 163, 313, 446, 510, 669
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 3 cut(s) 184, 594, 889
Lsp1109I GCAGC 2 cut(s) 65, 221
LweI GCATC 2 cut(s) 67, 757
MaeI CTAG 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 653
MalI GATC 6 cut(s) 71, 165, 315, 448, 512, 671
MboI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
MboII GAAGA 8 cut(s) 56, 79, 163, 187, 262, 335, 346, 550
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 5 cut(s) 347, 359, 460, 833, 875
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 1 cut(s) 699
Mph1103I ATGCAT 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 437
MspI CCGG 4 cut(s) 523, 703, 782, 788
MspR9I CCNGG 5 cut(s) 524, 563, 704, 782, 916
MvaI CCWGG 2 cut(s) 563, 916
MwoI GCNNNNNNNGC 1 cut(s) 911
NciI CCSGG 3 cut(s) 524, 704, 782
NcoI CCATGG 2 cut(s) 115, 180
NdeII GATC 6 cut(s) 69, 163, 313, 446, 510, 669
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 7 cut(s) 28, 84, 119, 184, 343, 495, 856
NlaIV GGNNCC 8 cut(s) 448, 456, 469, 527, 629, 727, 786, 891
NmuCI GTSAC 1 cut(s) 653
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 1 cut(s) 84
PaeR7I CTCGAG 1 cut(s) 317
PceI AGGCCT 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 743
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 1 cut(s) 403
PflFI GACNNNGTC 2 cut(s) 227, 572
PflMI CCANNNNNTGG 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 2 cut(s) 55, 211
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 561, 914
PspEI GGTNACC 1 cut(s) 653
PspGI CCWGG 2 cut(s) 561, 914
PspN4I GGNNCC 8 cut(s) 448, 456, 469, 527, 629, 727, 786, 891
PspOMI GGGCCC 1 cut(s) 525
PspPI GGNCC 4 cut(s) 455, 468, 525, 526
PsuI RGATCY 1 cut(s) 446
PsyI GACNNNGTC 2 cut(s) 227, 572
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
SapI GCTCTTC 1 cut(s) 563
SatI GCNGC 2 cut(s) 54, 210
Sau3AI GATC 6 cut(s) 69, 163, 313, 446, 510, 669
Sau96I GGNCC 4 cut(s) 455, 468, 525, 526
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 5 cut(s) 524, 563, 704, 782, 916
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 2 cut(s) 67, 757
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 455, 468
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 3 cut(s) 317, 413, 593
SmoI CTYRAG 3 cut(s) 317, 413, 593
Sse9I AATT 5 cut(s) 347, 359, 460, 833, 875
SseBI AGGCCT 1 cut(s) 219
SspMI CTAG 1 cut(s) 371
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 5 cut(s) 522, 561, 702, 780, 914
StyI CCWWGG 7 cut(s) 60, 115, 180, 450, 471, 623, 885
TaaI ACNGT 1 cut(s) 145
TaqI TCGA 3 cut(s) 222, 318, 737
TasI AATT 5 cut(s) 347, 359, 460, 833, 875
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 1 cut(s) 403
TscAI CASTG 3 cut(s) 148, 360, 583
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 53, 209
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 4 cut(s) 170, 356, 869, 923
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 3 cut(s) 148, 360, 583
Tth111I GACNNNGTC 2 cut(s) 227, 572
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 455, 468
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 4 cut(s) 347, 460, 833, 875
XceI RCATGY 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XhoI CTCGAG 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.