MD06G1015900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013635
Physical Location & Seq
Reverse (-)
1987926 .. 1990885
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1015900.v1.1.491

Sequence Viewer

Length: 597 bp
ATGAAGAAAAGCGTGGGAAGTGGAAATCGAACCCGGAGATGCATAACAGAAAAGTTTCTGGAAACGCGGTTACAATTTCCAGTGGCTTCTTCCCCTCCAAACCAGCCGTCACCGAAATCGCCAATTTTCAATGCCAGCAGCAGGCGCAAGAGGGCGGCCGTCGCTGCCGGAGGAGGAGCCTCAGCCTCTGTTGCGGCGGCGATCATGGAATCCCCCAAAAGTAACGCTTTAGCTTCGATTCCTGATCTCAAGCACCTCGCCTCCTCTCGTCTCCGCGATCTCAAGCTCCACGTCGACAGATCCCACTCTGAGATCCTCAAGGATTTTGATTCCTCTCAATCTCGCCTCCACAAGCGATTCAAGATCCAGAGCCAGGCATGCCAACAAGTGTTGGATGAAGCAGATAAGGAAAACAAGAAGATGACCCAAAGGATTACCGAAAGTCGGGAAGCAATGGCGGCTTCATATGCAGAGTTCATGGCAGATGCACAGACCACCGCTTCTCGTGCTTGCAAAACATCAATCGCTGAGCTTTCGCAGTCCTTTGAGAAACAAATCGATGCTCTTCGCAGCCGTTATGGCATGTCAACAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.76

Weight (kDa)

10.01

Isoelectric Point (pI)

73.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08760 FvH4_5g24040
malus_domestica MD06G1015900.v1.1 MD06G1016300.v1.1 MD06G1016600.v1.1
prunus_persica Prupe.5G019800_v2.0.a1
pyrus_communis pycom06g01280
rosa_chinensis RchiOBHm_Chr7g0213521
rosa_laevigata RLG00000002798
rosa_multiflora Rmu_sc0010076.1_g000002
rosa_roxburghii Rroxscaffold_3G00245810
rosa_rugosa Rorug07G0142700
rosa_samantha Rh7AG275900 Rh7BG270300 Rh7CG294800 Rh7DG282500
rosa_wichuraiana Rw0G023480 Rw7G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 294
AccII CGCG 2 cut(s) 67, 276
AciI CCGC 7 cut(s) 67, 155, 194, 197, 274, 458, 498
AclWI GGATC 3 cut(s) 294, 307, 358
AcoI YGGCCR 1 cut(s) 156
AfiI CCNNNNNNNGG 3 cut(s) 141, 373, 444
AgsI TTSAA 2 cut(s) 130, 361
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 3 cut(s) 233, 286, 532
AluI AGCT 3 cut(s) 233, 286, 532
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 3 cut(s) 294, 307, 358
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 138, 164, 570
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 147
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 102
BauI CACGAG 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 181
BbvI GCAGC 3 cut(s) 150, 151, 582
BceAI ACGGC 3 cut(s) 91, 143, 558
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 275
BglI GCCNNNNNGGC 1 cut(s) 579
BisI GCNGC 7 cut(s) 139, 156, 165, 195, 198, 459, 571
BlpI GCTNAGC 1 cut(s) 528
BlsI GCNGC 7 cut(s) 140, 157, 166, 196, 199, 460, 572
Bme1390I CCNGG 2 cut(s) 34, 374
BmgBI CACGTC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 2 cut(s) 34, 374
BmsI GCATC 3 cut(s) 29, 475, 550
Bpu10I CCTNAGC 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 528
BpuEI CTTGAG 3 cut(s) 233, 266, 302
BpuMI CCSGG 1 cut(s) 34
Bsa29I ATCGAT 1 cut(s) 558
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 373, 444
Bse1I ACTGG 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 1 cut(s) 558
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 3 cut(s) 141, 373, 444
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 3 cut(s) 195, 300, 519
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 3 cut(s) 186, 189, 253
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 150, 151, 582
Bsh1236I CGCG 2 cut(s) 67, 276
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 558
BsiEI CGRYCG 1 cut(s) 159
BsiSI CCGG 2 cut(s) 34, 168
BslI CCNNNNNNNGG 3 cut(s) 141, 373, 444
BsmAI GTCTC 1 cut(s) 275
BsmBI CGTCTC 1 cut(s) 275
BsnI GGCC 1 cut(s) 158
Bsp143I GATC 6 cut(s) 201, 244, 277, 299, 312, 363
Bsp1720I GCTNAGC 1 cut(s) 528
BspACI CCGC 7 cut(s) 67, 155, 194, 197, 274, 458, 498
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 3 cut(s) 194, 301, 520
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 2 cut(s) 67, 276
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 3 cut(s) 294, 307, 358
BspQI GCTCTTC 1 cut(s) 570
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 80
BssMI GATC 6 cut(s) 201, 244, 277, 299, 312, 363
BssSI CACGAG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 4 cut(s) 136, 143, 379, 511
BstDEI CTNAG 3 cut(s) 181, 309, 528
BstF5I GGATG 1 cut(s) 400
BstFNI CGCG 2 cut(s) 67, 276
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 6 cut(s) 204, 247, 280, 302, 315, 366
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 6 cut(s) 201, 244, 277, 299, 312, 363
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 9 cut(s) 144, 161, 164, 191, 378, 458, 467, 506, 579
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 2 cut(s) 381, 586
BstSCI CCNGG 2 cut(s) 32, 372
BstUI CGCG 2 cut(s) 67, 276
BstV1I GCAGC 3 cut(s) 150, 151, 582
BstX2I RGATCY 3 cut(s) 299, 312, 363
BstYI RGATCY 3 cut(s) 299, 312, 363
BstZI CGGCCG 1 cut(s) 156
Bsu15I ATCGAT 1 cut(s) 558
BsuRI GGCC 1 cut(s) 158
BsuTUI ATCGAT 1 cut(s) 558
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 400
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 4 cut(s) 136, 143, 379, 511
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 558
CviAII CATG 4 cut(s) 205, 378, 478, 583
DdeI CTNAG 3 cut(s) 181, 309, 528
DpnI GATC 6 cut(s) 203, 246, 279, 301, 314, 365
DpnII GATC 6 cut(s) 201, 244, 277, 299, 312, 363
EaeI YGGCCR 1 cut(s) 156
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EclXI CGGCCG 1 cut(s) 156
Eco52I CGGCCG 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 4 cut(s) 208, 381, 481, 586
FaiI YATR 8 cut(s) 44, 206, 379, 466, 468, 479, 579, 584
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FatI CATG 4 cut(s) 204, 377, 477, 582
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 7 cut(s) 139, 156, 165, 195, 198, 459, 571
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 7 cut(s) 139, 156, 165, 195, 198, 459, 571
GlaI GCGC 1 cut(s) 146
GluI GCNGC 7 cut(s) 139, 156, 165, 195, 198, 459, 571
HaeIII GGCC 1 cut(s) 158
HapII CCGG 2 cut(s) 34, 168
HhaI GCGC 1 cut(s) 147
Hin1II CATG 4 cut(s) 208, 381, 481, 586
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 2 cut(s) 295, 588
HindII GTYRAC 2 cut(s) 295, 588
HinfI GANTC 4 cut(s) 209, 238, 329, 357
HpaII CCGG 2 cut(s) 34, 168
HphI GGTGA 1 cut(s) 102
Hpy166II GTNNAC 2 cut(s) 295, 588
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 5 cut(s) 59, 242, 361, 367, 446
Hpy8I GTNNAC 2 cut(s) 295, 588
Hpy99I CGWCG 2 cut(s) 164, 296
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 4 cut(s) 42, 470, 488, 513
HpyF10VI GCNNNNNNNGC 9 cut(s) 144, 161, 164, 191, 378, 458, 467, 506, 579
HpyF3I CTNAG 3 cut(s) 181, 309, 528
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 4 cut(s) 208, 381, 481, 586
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 6 cut(s) 201, 244, 277, 299, 312, 363
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 2 cut(s) 176, 291
Lsp1109I GCAGC 3 cut(s) 150, 151, 582
LweI GCATC 3 cut(s) 29, 475, 550
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 3 cut(s) 69, 108, 221
MalI GATC 6 cut(s) 203, 246, 279, 301, 314, 365
MboI GATC 6 cut(s) 201, 244, 277, 299, 312, 363
MboII GAAGA 4 cut(s) 16, 81, 430, 557
MflI RGATCY 3 cut(s) 299, 312, 363
MluCI AATT 3 cut(s) 74, 123, 592
MmeI TCCRAC 1 cut(s) 372
Mph1103I ATGCAT 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 54
MspI CCGG 2 cut(s) 34, 168
MspR9I CCNGG 2 cut(s) 34, 374
MvaI CCWGG 1 cut(s) 374
MvnI CGCG 2 cut(s) 67, 276
MwoI GCNNNNNNNGC 9 cut(s) 144, 161, 164, 191, 378, 458, 467, 506, 579
NciI CCSGG 1 cut(s) 34
NdeI CATATG 1 cut(s) 466
NdeII GATC 6 cut(s) 201, 244, 277, 299, 312, 363
NlaIII CATG 4 cut(s) 208, 381, 481, 586
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 108
NsiI ATGCAT 1 cut(s) 44
NspI RCATGY 2 cut(s) 381, 586
PaeI GCATGC 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 4 cut(s) 209, 238, 329, 357
PkrI GCNGC 7 cut(s) 140, 157, 166, 196, 199, 460, 572
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 3 cut(s) 299, 312, 363
SalI GTCGAC 1 cut(s) 293
SapI GCTCTTC 1 cut(s) 570
SatI GCNGC 7 cut(s) 139, 156, 165, 195, 198, 459, 571
Sau3AI GATC 6 cut(s) 201, 244, 277, 299, 312, 363
ScrFI CCNGG 2 cut(s) 34, 374
SetI ASST 5 cut(s) 235, 258, 288, 294, 534
SfaNI GCATC 3 cut(s) 29, 475, 550
SmlI CTYRAG 3 cut(s) 248, 281, 317
SmoI CTYRAG 3 cut(s) 248, 281, 317
SphI GCATGC 1 cut(s) 381
Sse9I AATT 3 cut(s) 74, 123, 592
SsiI CCGC 7 cut(s) 67, 155, 194, 197, 274, 458, 498
StyD4I CCNGG 2 cut(s) 32, 372
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 28, 236, 294, 558
TasI AATT 3 cut(s) 74, 123, 592
TauI GCSGC 4 cut(s) 158, 197, 200, 461
TfiI GAWTC 4 cut(s) 209, 238, 329, 357
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 1 cut(s) 108
TseI GCWGC 3 cut(s) 138, 164, 570
Tsp45I GTSAC 1 cut(s) 108
TspDTI ATGAA 4 cut(s) 17, 411, 453, 466
TspRI CASTG 1 cut(s) 87
XceI RCATGY 2 cut(s) 381, 586
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.