MD06G1016600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013635
Physical Location & Seq
Reverse (-)
2051019 .. 2054195
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1016600.v1.1.491

Sequence Viewer

Length: 573 bp
ATGAAGAAAAGCAGCGGAAGCGGAAGCAGAAGCGGAAGTCGAACCCGGAGATGCTTAACCGCGAACTTTCTGGAAACGCGGTTACAATTTCCAGTGGTTTCTTCCCCTGCAAACCAACCGTCACCGAAATCGCCAACTTTCAATACCAGCAGCAAGCGCAAGAGGGCGGCCGTCGCTGCCGGAGGAGGAGCCTTGGCCTCTGTTGCTGCAGCGCTCATGGAATCCCCCAAAAGTAACGCTTTAGCTTCGATTCCTGATCTCAGGCTCCTCGCCTCCTCTTGTCTCCACGATCTCAAGCTGCATGTCGACCGCTCCCACTCTGAGATCCTCAAGGATGTTGATTCCTCTCAATCTCGCCTCCACAAGCGATTCAAGATCCAGAGCCAGGCATGCCAACAAGTGTTGGATGAAGCAGATAAGGAATACAAGAAGATGACCCAAAGGATTACTGAAAGTCGGGAAGCAATGGCGTGCTTATTCATCAAGTGCATCCTACTGCTTTTTTATCAGGCTTCATATGCAGAGTTCATGGCAGATGCACAGACCACCACTTCTCGTGGTTGCAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.79

Weight (kDa)

9.78

Isoelectric Point (pI)

73.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08760 FvH4_5g24040
malus_domestica MD06G1015900.v1.1 MD06G1016300.v1.1 MD06G1016600.v1.1
prunus_persica Prupe.5G019800_v2.0.a1
pyrus_communis pycom06g01280
rosa_chinensis RchiOBHm_Chr7g0213521
rosa_laevigata RLG00000002798
rosa_multiflora Rmu_sc0010076.1_g000002
rosa_roxburghii Rroxscaffold_3G00245810
rosa_rugosa Rorug07G0142700
rosa_samantha Rh7AG275900 Rh7BG270300 Rh7CG294800 Rh7DG282500
rosa_wichuraiana Rw0G023480 Rw7G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 306
AccII CGCG 2 cut(s) 62, 79
AciI CCGC 7 cut(s) 15, 21, 33, 60, 79, 167, 310
AclWI GGATC 2 cut(s) 319, 370
AcoI YGGCCR 1 cut(s) 168
AfeI AGCGCT 1 cut(s) 213
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 2 cut(s) 142, 373
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 2 cut(s) 245, 298
AluI AGCT 2 cut(s) 245, 298
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 2 cut(s) 319, 370
Aor51HI AGCGCT 1 cut(s) 213
AoxI GGCC 2 cut(s) 168, 195
ApeKI GCWGC 6 cut(s) 12, 150, 176, 206, 209, 298
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspLEI GCGC 2 cut(s) 159, 214
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 114
BauI CACGAG 1 cut(s) 555
BbvI GCAGC 6 cut(s) 24, 162, 163, 193, 221, 285
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 386
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 215
BisI GCNGC 7 cut(s) 13, 151, 168, 177, 207, 210, 299
BlsI GCNGC 7 cut(s) 14, 152, 169, 178, 208, 211, 300
Bme1390I CCNGG 2 cut(s) 46, 386
BmiI GGNNCC 2 cut(s) 190, 266
BmrFI CCNGG 2 cut(s) 46, 386
BmsI GCATC 3 cut(s) 41, 498, 526
BpuEI CTTGAG 2 cut(s) 278, 314
BpuMI CCSGG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 340, 412, 489
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 2 cut(s) 274, 312
BseNI ACTGG 1 cut(s) 92
BseRI GAGGAG 4 cut(s) 198, 201, 257, 265
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 6 cut(s) 24, 162, 163, 193, 221, 285
Bsh1236I CGCG 2 cut(s) 62, 79
Bsh1285I CGRYCG 2 cut(s) 171, 310
BshFI GGCC 2 cut(s) 170, 197
BsiEI CGRYCG 2 cut(s) 171, 310
BsiSI CCGG 2 cut(s) 46, 180
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 1 cut(s) 287
BsnI GGCC 2 cut(s) 170, 197
Bsp143I GATC 4 cut(s) 256, 289, 324, 375
BspACI CCGC 7 cut(s) 15, 21, 33, 60, 79, 167, 310
BspANI GGCC 2 cut(s) 170, 197
BspCNI CTCAG 2 cut(s) 273, 313
BspFNI CGCG 2 cut(s) 62, 79
BspLI GGNNCC 2 cut(s) 190, 266
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 2 cut(s) 319, 370
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 4 cut(s) 256, 289, 324, 375
BssSI CACGAG 1 cut(s) 555
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 155, 391, 472
BstDEI CTNAG 2 cut(s) 260, 321
BstF5I GGATG 3 cut(s) 340, 412, 489
BstFNI CGCG 2 cut(s) 62, 79
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 159, 214
BstKTI GATC 4 cut(s) 259, 292, 327, 378
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 4 cut(s) 256, 289, 324, 375
BstMCI CGRYCG 2 cut(s) 171, 310
BstMWI GCNNNNNNNGC 7 cut(s) 18, 156, 173, 176, 203, 390, 518
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 2 cut(s) 305, 393
BstSCI CCNGG 2 cut(s) 44, 384
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 2 cut(s) 62, 79
BstV1I GCAGC 6 cut(s) 24, 162, 163, 193, 221, 285
BstX2I RGATCY 2 cut(s) 324, 375
BstYI RGATCY 2 cut(s) 324, 375
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 2 cut(s) 170, 197
BtsCI GGATG 3 cut(s) 340, 412, 489
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 155, 391, 472
CfoI GCGC 2 cut(s) 159, 214
CviAII CATG 5 cut(s) 217, 302, 390, 529, 570
CviJI RGCY 8 cut(s) 170, 191, 197, 245, 265, 298, 384, 512
CviKI_1 RGCY 8 cut(s) 170, 191, 197, 245, 265, 298, 384, 512
DdeI CTNAG 2 cut(s) 260, 321
DpnI GATC 4 cut(s) 258, 291, 326, 377
DpnII GATC 4 cut(s) 256, 289, 324, 375
EaeI YGGCCR 1 cut(s) 168
EagI CGGCCG 1 cut(s) 168
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 192
Eco47III AGCGCT 1 cut(s) 213
Eco52I CGGCCG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 5 cut(s) 220, 305, 393, 532, 573
FaiI YATR 7 cut(s) 218, 303, 391, 517, 519, 530, 571
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FatI CATG 5 cut(s) 216, 301, 389, 528, 569
FauNDI CATATG 1 cut(s) 517
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 7 cut(s) 13, 151, 168, 177, 207, 210, 299
FokI GGATG 3 cut(s) 347, 419, 476
Fsp4HI GCNGC 7 cut(s) 13, 151, 168, 177, 207, 210, 299
GlaI GCGC 2 cut(s) 158, 213
GluI GCNGC 7 cut(s) 13, 151, 168, 177, 207, 210, 299
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 2 cut(s) 170, 197
HapII CCGG 2 cut(s) 46, 180
HhaI GCGC 2 cut(s) 159, 214
Hin1II CATG 5 cut(s) 220, 305, 393, 532, 573
Hin6I GCGC 2 cut(s) 157, 212
HinP1I GCGC 2 cut(s) 157, 212
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 4 cut(s) 221, 250, 341, 369
HpaII CCGG 2 cut(s) 46, 180
HphI GGTGA 1 cut(s) 114
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 5 cut(s) 71, 254, 373, 379, 458
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4V TGCA 7 cut(s) 110, 209, 301, 489, 521, 539, 564
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 156, 173, 176, 203, 390, 518
HpyF3I CTNAG 2 cut(s) 260, 321
Hsp92II CATG 5 cut(s) 220, 305, 393, 532, 573
HspAI GCGC 2 cut(s) 157, 212
Kzo9I GATC 4 cut(s) 256, 289, 324, 375
LmnI GCTCC 3 cut(s) 188, 270, 317
Lsp1109I GCAGC 6 cut(s) 24, 162, 163, 193, 221, 285
LweI GCATC 3 cut(s) 41, 498, 526
MaeIII GTNAC 3 cut(s) 81, 120, 233
MalI GATC 4 cut(s) 258, 291, 326, 377
MbiI CCGCTC 1 cut(s) 312
MboI GATC 4 cut(s) 256, 289, 324, 375
MboII GAAGA 3 cut(s) 16, 93, 442
MflI RGATCY 2 cut(s) 324, 375
MluCI AATT 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 384
MseI TTAA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 2 cut(s) 46, 180
MspR9I CCNGG 2 cut(s) 46, 386
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 2 cut(s) 62, 79
MwoI GCNNNNNNNGC 7 cut(s) 18, 156, 173, 176, 203, 390, 518
NciI CCSGG 1 cut(s) 46
NdeI CATATG 1 cut(s) 517
NdeII GATC 4 cut(s) 256, 289, 324, 375
NlaIII CATG 5 cut(s) 220, 305, 393, 532, 573
NlaIV GGNNCC 2 cut(s) 190, 266
NmuCI GTSAC 1 cut(s) 120
NspI RCATGY 2 cut(s) 305, 393
PaeI GCATGC 1 cut(s) 393
PfeI GAWTC 4 cut(s) 221, 250, 341, 369
PkrI GCNGC 7 cut(s) 14, 152, 169, 178, 208, 211, 300
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 190, 266
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 324, 375
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 7 cut(s) 13, 151, 168, 177, 207, 210, 299
Sau3AI GATC 4 cut(s) 256, 289, 324, 375
ScrFI CCNGG 2 cut(s) 46, 386
SetI ASST 2 cut(s) 247, 300
SfaNI GCATC 3 cut(s) 41, 498, 526
SfcI CTRYAG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 293, 329
SmoI CTYRAG 2 cut(s) 293, 329
SphI GCATGC 1 cut(s) 393
Sse9I AATT 1 cut(s) 86
SsiI CCGC 7 cut(s) 15, 21, 33, 60, 79, 167, 310
StyD4I CCNGG 2 cut(s) 44, 384
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 40, 248, 306
TasI AATT 1 cut(s) 86
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 4 cut(s) 221, 250, 341, 369
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 6 cut(s) 12, 150, 176, 206, 209, 298
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 5 cut(s) 17, 423, 469, 504, 517
TspRI CASTG 1 cut(s) 99
XceI RCATGY 2 cut(s) 305, 393
XmiI GTMKAC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.