Rorug07G0142700

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000644
Physical Location & Seq
Reverse (-)
11263082 .. 11265561
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0142700.1

Sequence Viewer

Length: 1038 bp
ATGGAGGAGAACAAAGTCTCACGAGTTTGTGTTACAGGTGGTTCTGGATATATTGGCTGTTGGCTAGTAAAAACGCTCCTAGAGAAGGGCCACACAGTCCATGCCACTCTCAGAAACTTGGAGGATGCATCAAAAGTAGGCCTTCTGAAGTCTCTCCCCAGTGCAGACACCAATTTGTTGCTGTTCAAAGCTGATATATACGATCCTAATGAGTTCGAGCCTGCAATTGAAGGGTGTGAGTTTGTTTTTCATGTTGCAACTCCCATGCAACATGATCCCCAAAACTCCCAGTACAAAGACACAGCTGAAGCTGCTGTTGCCGGGGTGAGAAGCATTGCTGATTCTTGCATTCGATCGCAGACAGTTAAGCGACTCATCTACACTGCAAGCATACTTTCTATGTCGCCAAGAAAGGATGATGGAGTTGGATTCAGACCCTTACTTGATGAGTCATGTTGGACACCACTTGATGCCTCATCTACTTATGGCAATGCTATCACGATGGGGTACATTAAATCAAAGACCTTAGCTGAGAAAGCAGTGCTGAGCCACAATGACTATGATGATAGTAAATTAGAAGTGGTAACTCTTCCTTGTGGCTTAGTGGGAGGAGAAAGTCTTCTTTCATATTTGCCTACAAGTGTGGGAGTGATTCTGGCACAACTTACTGGGGACTTGTTTTCTTGCAATGCCTTAAAATTAATGCAAGAATTTATGGGATCAGTTCCTCTGGTGCACATTGATGATGTTTGCCAAGCACATATCTTCTGCATGGAACAGCCATCAATGAAAGGCAGATTCTGTTGTGCAGTTGCCTGTCACTCTATCAAAGAAATTGCAATATACTTCCAAGAAACTTACCCAGAATACAAGATACAAAAAGAATTGACCGAAGGGCCTGAAGAAGGAAGCCAAAGTGATTTCAGTAAGCTGAAGAAGATGGGCTTTGAGTATAAGTATGGCATGAAGGACATACTAGATGACAGTGTAGCATGGGCAAAGAAGTTAAATGGGAGCTCTCTTTCTCAAGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

345

Amino Acids

37.92

Weight (kDa)

5.26

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 109 2.4e-10 NmrA-like family
Epimerase PF01370 9 - 262 1.5e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 199 4.1e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 2.1e-10 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 209 6.4e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 132 4.3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 197, 269, 727
AcsI RAATTY 1 cut(s) 710
AcuI CTGAAG 4 cut(s) 167, 327, 921, 953
AfaI GTAC 2 cut(s) 293, 509
AfiI CCNNNNNNNGG 2 cut(s) 85, 905
AgsI TTSAA 2 cut(s) 187, 230
AluBI AGCT 6 cut(s) 191, 305, 311, 530, 931, 1017
AluI AGCT 6 cut(s) 191, 305, 311, 530, 931, 1017
Alw21I GWGCWC 2 cut(s) 738, 1019
Alw26I GTCTC 2 cut(s) 22, 156
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 3 cut(s) 197, 269, 727
AlwNI CAGNNNCTG 1 cut(s) 801
AoxI GGCC 3 cut(s) 88, 139, 896
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 710
AseI ATTAAT 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 88, 896
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 738
BanII GRGCYC 1 cut(s) 1019
BauI CACGAG 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 738, 1019
BbvI GCAGC 1 cut(s) 298
BccI CCATC 4 cut(s) 413, 496, 790, 934
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 22, 156
BfaI CTAG 3 cut(s) 65, 80, 977
BisI GCNGC 1 cut(s) 312
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 88, 896
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 3 cut(s) 153, 283, 678
BmsI GCATC 3 cut(s) 115, 137, 460
BmuI ACTGGG 3 cut(s) 153, 283, 678
BpiI GAAGAC 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 1011
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 321
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 905
Bse1I ACTGG 3 cut(s) 159, 289, 673
Bse3DI GCAATG 3 cut(s) 333, 496, 694
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 2 cut(s) 130, 421
BseLI CCNNNNNNNGG 2 cut(s) 85, 905
BseMI GCAATG 3 cut(s) 333, 496, 694
BseMII CTCAG 3 cut(s) 124, 522, 536
BseNI ACTGG 3 cut(s) 159, 289, 673
BseRI GAGGAG 2 cut(s) 20, 624
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 1 cut(s) 298
BsgI GTGCAG 2 cut(s) 183, 828
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 90, 141, 898
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 2 cut(s) 738, 1019
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 2 cut(s) 85, 905
BsmAI GTCTC 2 cut(s) 22, 156
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 3 cut(s) 90, 141, 898
Bsp1286I GDGCHC 2 cut(s) 738, 1019
Bsp143I GATC 4 cut(s) 202, 274, 353, 719
Bsp1720I GCTNAGC 1 cut(s) 545
BspANI GGCC 3 cut(s) 90, 141, 898
BspCNI CTCAG 3 cut(s) 123, 523, 537
BspPI GGATC 3 cut(s) 197, 269, 727
BsrDI GCAATG 3 cut(s) 333, 496, 694
BsrI ACTGG 3 cut(s) 159, 289, 673
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 4 cut(s) 202, 274, 353, 719
BssSI CACGAG 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 21
Bst4CI ACNGT 3 cut(s) 97, 364, 986
Bst6I CTCTTC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 222, 388
BstDEI CTNAG 5 cut(s) 110, 526, 531, 545, 601
BstENI CCTNNNNNAGG 2 cut(s) 83, 903
BstF5I GGATG 2 cut(s) 130, 421
BstKTI GATC 4 cut(s) 205, 277, 356, 722
BstMAI GTCTC 2 cut(s) 22, 156
BstMBI GATC 4 cut(s) 202, 274, 353, 719
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 3 cut(s) 311, 317, 536
BstSCI CCNGG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 738
BstV1I GCAGC 1 cut(s) 298
BstV2I GAAGAC 1 cut(s) 611
BsuRI GGCC 3 cut(s) 90, 141, 898
BtsCI GGATG 2 cut(s) 130, 421
BtsI GCAGTG 2 cut(s) 381, 546
BtsIMutI CAGTG 4 cut(s) 166, 381, 546, 991
Cac8I GCNNGC 2 cut(s) 222, 388
CaiI CAGNNNCTG 1 cut(s) 801
Cfr13I GGNCC 2 cut(s) 88, 896
Csp6I GTAC 2 cut(s) 292, 508
CviAII CATG 8 cut(s) 101, 251, 265, 272, 453, 772, 964, 993
CviQI GTAC 2 cut(s) 292, 508
DdeI CTNAG 5 cut(s) 110, 526, 531, 545, 601
DpnI GATC 4 cut(s) 204, 276, 355, 721
DpnII GATC 4 cut(s) 202, 274, 353, 719
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 1017
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 1019
Eco53kI GAGCTC 1 cut(s) 1017
Eco57I CTGAAG 4 cut(s) 167, 327, 921, 953
EcoICRI GAGCTC 1 cut(s) 1017
EcoNI CCTNNNNNAGG 2 cut(s) 83, 903
EcoO109I RGGNCCY 1 cut(s) 896
EcoT22I ATGCAT 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 1019
FaeI CATG 8 cut(s) 104, 254, 268, 275, 456, 775, 967, 996
FalI AAGNNNNNCTT 4 cut(s) 126, 158, 929, 961
FaqI GGGAC 1 cut(s) 686
FatI CATG 8 cut(s) 100, 250, 264, 271, 452, 771, 963, 992
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 137, 428
FriOI GRGCYC 1 cut(s) 1019
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 3 cut(s) 65, 80, 977
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 3 cut(s) 90, 141, 898
HapII CCGG 1 cut(s) 321
Hin1II CATG 8 cut(s) 104, 254, 268, 275, 456, 775, 967, 996
HinfI GANTC 6 cut(s) 341, 372, 429, 449, 652, 798
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 3 cut(s) 113, 147, 434
Hpy188III TCNNGA 3 cut(s) 21, 45, 499
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 6 cut(s) 79, 152, 224, 887, 899, 961
HpyCH4III ACNGT 3 cut(s) 97, 364, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 317, 536
HpyF3I CTNAG 5 cut(s) 110, 526, 531, 545, 601
Hsp92II CATG 8 cut(s) 104, 254, 268, 275, 456, 775, 967, 996
Kzo9I GATC 4 cut(s) 202, 274, 353, 719
LmnI GCTCC 2 cut(s) 81, 1014
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 3 cut(s) 115, 137, 460
MaeI CTAG 3 cut(s) 65, 80, 977
MaeIII GTNAC 3 cut(s) 31, 583, 818
MalI GATC 4 cut(s) 204, 276, 355, 721
MboI GATC 4 cut(s) 202, 274, 353, 719
MboII GAAGA 6 cut(s) 581, 611, 757, 914, 946, 949
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 738, 1019
MluCI AATT 7 cut(s) 172, 225, 572, 698, 710, 834, 884
MlyI GAGTC 2 cut(s) 366, 458
MmeI TCCRAC 2 cut(s) 406, 437
MnlI CCTC 4 cut(s) 115, 484, 602, 738
Mph1103I ATGCAT 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 618
MseI TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
MslI CAYNNNNRTG 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 311, 317, 536
NciI CCSGG 1 cut(s) 322
NdeII GATC 4 cut(s) 202, 274, 353, 719
NlaIII CATG 8 cut(s) 104, 254, 268, 275, 456, 775, 967, 996
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 130
PceI AGGCCT 1 cut(s) 141
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 341, 429, 652, 798
PkrI GCNGC 1 cut(s) 313
Ple19I CGATCG 1 cut(s) 356
PleI GAGTC 2 cut(s) 366, 457
PpsI GAGTC 2 cut(s) 366, 457
PshBI ATTAAT 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 1019
PspPI GGNCC 2 cut(s) 88, 896
PstNI CAGNNNCTG 1 cut(s) 801
PvuI CGATCG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 293, 509
RsaNI GTAC 2 cut(s) 292, 508
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 1 cut(s) 1019
SaqAI TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 202, 274, 353, 719
Sau96I GGNCC 2 cut(s) 88, 896
SchI GAGTC 2 cut(s) 366, 458
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 738, 1019
SetI ASST 8 cut(s) 40, 193, 307, 313, 527, 532, 933, 1019
SfaNI GCATC 3 cut(s) 115, 137, 460
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 1026
SmoI CTYRAG 1 cut(s) 1026
Sse9I AATT 7 cut(s) 172, 225, 572, 698, 710, 834, 884
SseBI AGGCCT 1 cut(s) 141
SspMI CTAG 3 cut(s) 65, 80, 977
SstI GAGCTC 1 cut(s) 1019
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 97, 364, 986
TaqI TCGA 2 cut(s) 216, 352
TaqII GACCGA 1 cut(s) 905
TasI AATT 7 cut(s) 172, 225, 572, 698, 710, 834, 884
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 4 cut(s) 341, 429, 652, 798
Tru1I TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
Tru9I TTAA 6 cut(s) 366, 513, 695, 701, 1007, 1036
TscAI CASTG 4 cut(s) 166, 388, 546, 991
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 4 cut(s) 239, 615, 803, 980
TspRI CASTG 4 cut(s) 166, 388, 546, 991
VneI GTGCAC 1 cut(s) 734
VspI ATTAAT 1 cut(s) 701
XagI CCTNNNNNAGG 2 cut(s) 83, 903
XapI RAATTY 1 cut(s) 710
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 3 cut(s) 65, 80, 977
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.