AT1G57820

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000644
Physical Location & Seq
Reverse (-)
21413981 .. 21418115
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G57820.1

Sequence Viewer

Length: 1938 bp
ATGGCGCGTGACATCCAACTCCCCTGCGACGGCGACGGCGTATGCATGCGATGCAAATCCAACCCTCCGCCTGAAGAATCTCTCACTTGCGGCACGTGCGTTACACCGTGGCACGTGTCCTGTCTCTCTTCACCTCCCAAAACCCTAGCTTCCACTCTACAGTGGCATTGTCCTGATTGCTCCGGCGAAATCGATCCTCTTCCTGTTTCCGGCGGTGCTACTGGTTTCGAATCTGCTGGGTCAGATCTTGTAGCTGCGATTCGTGCGATTGAGGCTGATGAGTCGTTGAGTACTGAAGAGAAAGCTAAGATGAGGCAACGGTTACTGAGTGGTAAAGGTGTTGAGGAGGATGATGAAGAAGAGAAGAGGAAGAAGAAGGGGAAAGGGAAGAATCCGAATCTGGATGTGTTATCTGCTCTTGGAGATAACTTGATGTGTTCTTTCTGTATGCAGTTGCCTGAGAGACCTGTGACGAAACCATGTGGGCACAACGCTTGCTTAAAATGTTTTGAGAAATGGATGGGGCAGGGAAAGAGAACTTGTGGTAAATGCCGCAGCATAATTCCTGAAAAAATGGCTAAGAATCCCCGTATCAACTCGTCTCTTGTTGCTGCCATTCGATTAGCAAAAGTCTCTAAAAGTGCTGCTGCGACCACTTCAAAGGTCTTTCATTTCATCAGCAACCAAGACCGACCAGATAAAGCATTTACAACCGAGCGTGCAAAGAAAACTGGCAAGGCAAACGCTGCTAGTGGAAAGATTTATGTTACAATACCACCAGATCATTTTGGTCCTATTCCAGCTGAAAATGACCCTGTCAGGAACCAAGGTCTTTTGGTTGGAGAATCCTGGGAGGACAGACTCGAGTGTAGGCAGTGGGGTGCTCATTTCCCACATGTTGCTGGCATTGCTGGACAATCTACTTATGGCGCTCAATCTGTAGCACTCTCTGGAGGTTATAAGGATGATGAGGATCATGGAGAATGGTTTCTATACACAGGAAGTGGGGGTAGAGATCTCAGTGGCAACAAAAGGACTAACAAGGAGCAGTCTTTTGACCAAAAGTTTGAGAAGTCTAATGCAGCATTAAAACTCAGCTGCAAATTGGGGTATCCTGTTCGAGTTGTCAGGTCTCACAAGGAGAAGCGTTCTGCATACGCCCCTGAGGAAGGAGTGAGATATGATGGGGTTTACAGGATTGAGAAGTGCTGGCGCAAAGTTGGAGTACAGGGTTCTTTTAAGGTCTGTCGTTACCTGTTCGTTAGATGTGACAATGAGCCAGCTCCATGGACCAGTGATGAGAATGGAGATCGTCCAAGACCTATCCCTAATATTCCAGAGCTTAATATGGCCACCGACCTGTTTGAGAGAAAAGAAACTCCATCATGGGATTTTGATGAAGGTGAGGGTTGTTGGAAATGGATGAAGCCGCCACCTGCAAGTAAAAAGTCAGTGAATGTTTTGGCTCCTGAGGAGAGGAAAAATTTGAGGAAAGCCATAAAGGCGGCACACTCGAATACCATGAGGGCAAGACTTCTGAAAGAATTTAAGTGCCAGATCTGTCAGCAAGTGTTGACTCTTCCTGTGACAACACCCTGTGCTCATAACTTCTGCAAGGCTTGCTTAGAAGCGAAATTTGCTGGGAAAACTCTTGTGAGAGAGAGAAGCACAGGTGGACGGACACTACGCTCAAGGAAGAATGTCCTGAACTGCCCTTGTTGCCCAACAGACATATCTGATTTTCTGCAGAACCCTCAGGTCAACAGAGAAGTAGCGGAGGTGATAGAGAAGCTAAAGACCCAGGAAGAGGACACCGCAGAGCTTGAAGATGAAGATGAAGGTGAATGCTCAGGCACCACCCCTGAAGAAGATTCTGAGCAACCGAAGAAAAGGATCAAGTTGGACACTGACGCAACAGTTTCTGCGACCATCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

645

Amino Acids

71.47

Weight (kDa)

8.36

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 143 - 188 9.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 146 - 184 4.9e-06 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 275 - 425 2.1e-48 SAD/SRA domain
zf-C3HC4_2 PF13923 517 - 543 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 518 - 546 1.3e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AarI CACCTGC 1 cut(s) 1444
Acc36I ACCTGC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 1853
AccII CGCG 1 cut(s) 7
AciI CCGC 8 cut(s) 68, 90, 213, 553, 1430, 1505, 1775, 1815
AclWI GGATC 3 cut(s) 188, 981, 1901
AcoI YGGCCR 1 cut(s) 1350
AcsI RAATTY 3 cut(s) 1483, 1544, 1634
AcuI CTGAAG 3 cut(s) 93, 315, 1884
AcvI CACGTG 2 cut(s) 96, 115
AdeI CACNNNGTG 1 cut(s) 1598
AfaI GTAC 2 cut(s) 292, 1227
AfiI CCNNNNNNNGG 4 cut(s) 29, 209, 1169, 1807
AflIII ACRYGT 2 cut(s) 114, 895
AgsI TTSAA 2 cut(s) 660, 1826
AjnI CCWGG 2 cut(s) 848, 1800
AluBI AGCT 9 cut(s) 149, 254, 305, 803, 1098, 1283, 1342, 1792, 1822
AluI AGCT 9 cut(s) 149, 254, 305, 803, 1098, 1283, 1342, 1792, 1822
Alw21I GWGCWC 2 cut(s) 886, 1603
Alw26I GTCTC 5 cut(s) 128, 457, 606, 637, 1137
AlwI GGATC 3 cut(s) 188, 981, 1901
AlwNI CAGNNNCTG 1 cut(s) 1922
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 1 cut(s) 1350
ApeKI GCWGC 8 cut(s) 254, 555, 611, 644, 647, 746, 1082, 1098
ApoI RAATTY 3 cut(s) 1483, 1544, 1634
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
Asp700I GAANNNNTTC 1 cut(s) 987
AspLEI GCGC 3 cut(s) 7, 932, 1215
AspS9I GGNCC 2 cut(s) 791, 1290
AsuHPI GGTGA 4 cut(s) 123, 1415, 1792, 1853
AsuII TTCGAA 1 cut(s) 228
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 2 cut(s) 791, 1290
AxyI CCTNAGG 3 cut(s) 1164, 1470, 1755
BaeGI GKGCMC 1 cut(s) 489
BalI TGGCCA 1 cut(s) 1352
BanI GGYRCC 1 cut(s) 1853
BbrPI CACGTG 2 cut(s) 96, 115
Bbv12I GWGCWC 2 cut(s) 886, 1603
BbvI GCAGC 8 cut(s) 241, 567, 598, 631, 634, 733, 1085, 1094
BccI CCATC 4 cut(s) 514, 1178, 1390, 1937
BceAI ACGGC 2 cut(s) 46, 52
BciT130I CCWGG 2 cut(s) 850, 1802
BciVI GTATCC 1 cut(s) 1122
BcoDI GTCTC 5 cut(s) 128, 457, 606, 637, 1137
BfaI CTAG 2 cut(s) 146, 750
BfmI CTRYAG 3 cut(s) 158, 939, 1745
BfoI RGCGCY 1 cut(s) 933
BfuAI ACCTGC 1 cut(s) 1444
BfuI GTATCC 1 cut(s) 1122
BglI GCCNNNNNGGC 1 cut(s) 1502
BglII AGATCT 3 cut(s) 244, 1015, 1557
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 850, 1802
Bme18I GGWCC 2 cut(s) 791, 1290
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 791, 1290
BmiI GGNNCC 3 cut(s) 824, 1467, 1855
BmrFI CCNGG 2 cut(s) 850, 1802
BmsI GCATC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 972
Bpu10I CCTNAGC 1 cut(s) 1849
Bpu14I TTCGAA 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 1675
Bsa29I ATCGAT 1 cut(s) 192
BsaAI YACGTR 2 cut(s) 96, 115
BsaBI GATNNNNATC 2 cut(s) 55, 972
BsaI GGTCTC 2 cut(s) 457, 1137
BsaJI CCNNGG 5 cut(s) 107, 826, 849, 1286, 1800
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 209, 1169, 1807
Bse1I ACTGG 3 cut(s) 226, 736, 1293
Bse21I CCTNAGG 3 cut(s) 1164, 1470, 1755
Bse3DI GCAATG 1 cut(s) 906
Bse8I GATNNNNATC 2 cut(s) 55, 972
BseBI CCWGG 2 cut(s) 850, 1802
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 5 cut(s) 107, 826, 849, 1286, 1800
BseGI GGATG 6 cut(s) 12, 355, 409, 525, 970, 1428
BseJI GATNNNNATC 2 cut(s) 55, 972
BseLI CCNNNNNNNGG 4 cut(s) 29, 209, 1169, 1807
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 9 cut(s) 317, 450, 1033, 1108, 1155, 1461, 1769, 1863, 1866
BseNI ACTGG 3 cut(s) 226, 736, 1293
BseRI GAGGAG 2 cut(s) 359, 1487
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 8 cut(s) 241, 567, 598, 631, 634, 733, 1085, 1094
BseYI CCCAGC 2 cut(s) 236, 1640
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 1352
BshNI GGYRCC 1 cut(s) 1853
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 886, 1603
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 2 cut(s) 183, 210
BslI CCNNNNNNNGG 4 cut(s) 29, 209, 1169, 1807
BsmAI GTCTC 5 cut(s) 128, 457, 606, 637, 1137
BsmBI CGTCTC 1 cut(s) 606
BsmI GAATGC 1 cut(s) 1850
BsnI GGCC 1 cut(s) 1352
Bso31I GGTCTC 2 cut(s) 457, 1137
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 3 cut(s) 489, 886, 1603
Bsp143I GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
Bsp19I CCATGG 1 cut(s) 1286
BspACI CCGC 8 cut(s) 68, 90, 213, 553, 1430, 1505, 1775, 1815
BspANI GGCC 1 cut(s) 1352
BspCNI CTCAG 9 cut(s) 318, 451, 1032, 1107, 1156, 1462, 1768, 1862, 1867
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 824, 1467, 1855
BspMAI CTGCAG 1 cut(s) 1749
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 3 cut(s) 188, 981, 1901
BspT104I TTCGAA 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 1853
BspTNI GGTCTC 2 cut(s) 457, 1137
BsrDI GCAATG 1 cut(s) 906
BsrI ACTGG 3 cut(s) 226, 736, 1293
BssECI CCNNGG 5 cut(s) 107, 826, 849, 1286, 1800
BssMI GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
BssT1I CCWWGG 2 cut(s) 826, 1286
Bst2UI CCWGG 2 cut(s) 850, 1802
Bst4CI ACNGT 4 cut(s) 108, 162, 321, 1918
Bst6I CTCTTC 7 cut(s) 133, 204, 291, 354, 359, 1584, 1800
BstAPI GCANNNNNTGC 3 cut(s) 51, 746, 1620
BstBAI YACGTR 2 cut(s) 96, 115
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 7 cut(s) 47, 496, 720, 904, 1211, 1281, 1621
BstDSI CCRYGG 2 cut(s) 107, 1286
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 6 cut(s) 12, 355, 409, 525, 970, 1428
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 933
BstHHI GCGC 3 cut(s) 7, 932, 1215
BstKTI GATC 8 cut(s) 196, 247, 784, 976, 1018, 1312, 1560, 1896
BstMAI GTCTC 5 cut(s) 128, 457, 606, 637, 1137
BstMBI GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
BstNI CCWGG 2 cut(s) 850, 1802
BstNSI RCATGY 2 cut(s) 49, 899
BstSCI CCNGG 2 cut(s) 848, 1800
BstSFI CTRYAG 3 cut(s) 158, 939, 1745
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 8 cut(s) 241, 567, 598, 631, 634, 733, 1085, 1094
BstX2I RGATCY 3 cut(s) 244, 1015, 1557
BstXI CCANNNNNNTGG 1 cut(s) 1287
BstYI RGATCY 3 cut(s) 244, 1015, 1557
Bsu15I ATCGAT 1 cut(s) 192
Bsu36I CCTNAGG 3 cut(s) 1164, 1470, 1755
BsuI GTATCC 1 cut(s) 1122
BsuRI GGCC 1 cut(s) 1352
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 2 cut(s) 107, 1286
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 6 cut(s) 12, 355, 409, 525, 970, 1428
BtsI GCAGTG 1 cut(s) 881
BtsIMutI CAGTG 6 cut(s) 167, 881, 1027, 1300, 1458, 1905
BveI ACCTGC 1 cut(s) 1444
Cac8I GCNNGC 7 cut(s) 47, 496, 720, 904, 1211, 1281, 1621
CaiI CAGNNNCTG 1 cut(s) 1922
CfoI GCGC 3 cut(s) 7, 932, 1215
Cfr13I GGNCC 2 cut(s) 791, 1290
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 1919
Csp6I GTAC 2 cut(s) 291, 1226
CviAII CATG 7 cut(s) 46, 480, 896, 977, 1287, 1386, 1522
CviQI GTAC 2 cut(s) 291, 1226
DpnI GATC 8 cut(s) 195, 246, 783, 975, 1017, 1311, 1559, 1895
DpnII GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
DraIII CACNNNGTG 1 cut(s) 1598
EaeI YGGCCR 1 cut(s) 1350
Eam1104I CTCTTC 7 cut(s) 133, 204, 291, 354, 359, 1584, 1800
EarI CTCTTC 7 cut(s) 133, 204, 291, 354, 359, 1584, 1800
EciI GGCGGA 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 826, 1286
Eco31I GGTCTC 2 cut(s) 457, 1137
Eco47I GGWCC 2 cut(s) 791, 1290
Eco57I CTGAAG 3 cut(s) 93, 315, 1884
Eco72I CACGTG 2 cut(s) 96, 115
Eco81I CCTNAGG 3 cut(s) 1164, 1470, 1755
Eco88I CYCGRG 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoRII CCWGG 2 cut(s) 848, 1800
EcoT14I CCWWGG 2 cut(s) 826, 1286
EcoT22I ATGCAT 1 cut(s) 47
ErhI CCWWGG 2 cut(s) 826, 1286
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 7 cut(s) 49, 483, 899, 980, 1290, 1389, 1525
FalI AAGNNNNNCTT 2 cut(s) 1607, 1639
FatI CATG 7 cut(s) 45, 479, 895, 976, 1286, 1385, 1521
FokI GGATG 5 cut(s) 362, 416, 532, 977, 1435
FspBI CTAG 2 cut(s) 146, 750
GlaI GCGC 3 cut(s) 6, 931, 1214
GsaI CCCAGC 2 cut(s) 240, 1644
GsuI CTGGAG 1 cut(s) 972
HaeII RGCGCY 1 cut(s) 933
HaeIII GGCC 1 cut(s) 1352
HapII CCGG 2 cut(s) 183, 210
HgaI GACGC 1 cut(s) 1919
HhaI GCGC 3 cut(s) 7, 932, 1215
Hin1II CATG 7 cut(s) 49, 483, 899, 980, 1290, 1389, 1525
Hin6I GCGC 3 cut(s) 5, 930, 1213
HinP1I GCGC 3 cut(s) 5, 930, 1213
HincII GTYRAC 2 cut(s) 1575, 1762
HindII GTYRAC 2 cut(s) 1575, 1762
HpaII CCGG 2 cut(s) 183, 210
HphI GGTGA 4 cut(s) 123, 1415, 1792, 1853
Hpy166II GTNNAC 4 cut(s) 1192, 1575, 1676, 1762
Hpy188I TCNGA 5 cut(s) 244, 396, 1539, 1738, 1876
Hpy188III TCNNGA 8 cut(s) 173, 401, 566, 820, 951, 1337, 1469, 1705
Hpy8I GTNNAC 4 cut(s) 1192, 1575, 1676, 1762
Hpy99I CGWCG 2 cut(s) 32, 38
HpyAV CCTTC 4 cut(s) 370, 1163, 1394, 1832
HpyCH4III ACNGT 4 cut(s) 108, 162, 321, 1918
HpyCH4IV ACGT 2 cut(s) 95, 114
HpySE526I ACGT 2 cut(s) 95, 114
Hsp92II CATG 7 cut(s) 49, 483, 899, 980, 1290, 1389, 1525
HspAI GCGC 3 cut(s) 5, 930, 1213
Kzo9I GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
LmnI GCTCC 4 cut(s) 185, 1045, 1288, 1471
Lsp1109I GCAGC 8 cut(s) 241, 567, 598, 631, 634, 733, 1085, 1094
LweI GCATC 1 cut(s) 41
MaeI CTAG 2 cut(s) 146, 750
MaeII ACGT 2 cut(s) 95, 114
MaeIII GTNAC 8 cut(s) 8, 100, 321, 469, 766, 1250, 1268, 1585
MalI GATC 8 cut(s) 195, 246, 783, 975, 1017, 1311, 1559, 1895
MboI GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
MflI RGATCY 3 cut(s) 244, 1015, 1557
MhlI GDGCHC 3 cut(s) 489, 886, 1603
MlsI TGGCCA 1 cut(s) 1352
MluCI AATT 5 cut(s) 561, 1103, 1483, 1544, 1634
MluNI TGGCCA 1 cut(s) 1352
MlyI GAGTC 3 cut(s) 290, 855, 1570
MmeI TCCRAC 6 cut(s) 40, 84, 820, 1201, 1394, 1881
Mox20I TGGCCA 1 cut(s) 1352
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 987
MscI TGGCCA 1 cut(s) 1352
MseI TTAA 5 cut(s) 500, 1088, 1239, 1344, 1548
Msp20I TGGCCA 1 cut(s) 1352
MspA1I CMGCKG 2 cut(s) 803, 1098
MspI CCGG 2 cut(s) 183, 210
MspR9I CCNGG 2 cut(s) 850, 1802
Mva1269I GAATGC 1 cut(s) 1850
MvaI CCWGG 2 cut(s) 850, 1802
MvnI CGCG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 1286
NdeII GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
NlaIII CATG 7 cut(s) 49, 483, 899, 980, 1290, 1389, 1525
NlaIV GGNNCC 3 cut(s) 824, 1467, 1855
NmuCI GTSAC 4 cut(s) 8, 469, 1268, 1585
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 899
NspV TTCGAA 1 cut(s) 228
PaeI GCATGC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 863
PaqCI CACCTGC 1 cut(s) 1444
PciI ACATGT 1 cut(s) 895
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1850
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 8 cut(s) 77, 230, 259, 391, 397, 583, 845, 1871
PflFI GACNNNGTC 1 cut(s) 815
PleI GAGTC 3 cut(s) 289, 855, 1570
PmaCI CACGTG 2 cut(s) 96, 115
PmlI CACGTG 2 cut(s) 96, 115
PpsI GAGTC 3 cut(s) 289, 855, 1570
Ppu21I YACGTR 2 cut(s) 96, 115
PscI ACATGT 1 cut(s) 895
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 848, 1800
PspCI CACGTG 2 cut(s) 96, 115
PspFI CCCAGC 2 cut(s) 236, 1640
PspGI CCWGG 2 cut(s) 848, 1800
PspN4I GGNNCC 3 cut(s) 824, 1467, 1855
PspPI GGNCC 2 cut(s) 791, 1290
PspXI VCTCGAGB 1 cut(s) 863
PstI CTGCAG 1 cut(s) 1749
PstNI CAGNNNCTG 1 cut(s) 1922
PsuI RGATCY 3 cut(s) 244, 1015, 1557
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 2 cut(s) 803, 1098
RsaI GTAC 2 cut(s) 292, 1227
RsaNI GTAC 2 cut(s) 291, 1226
SaqAI TTAA 5 cut(s) 500, 1088, 1239, 1344, 1548
Sau3AI GATC 8 cut(s) 193, 244, 781, 973, 1015, 1309, 1557, 1893
Sau96I GGNCC 2 cut(s) 791, 1290
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 3 cut(s) 290, 855, 1570
ScrFI CCNGG 2 cut(s) 850, 1802
SduI GDGCHC 3 cut(s) 489, 886, 1603
SfaNI GCATC 1 cut(s) 41
SfcI CTRYAG 3 cut(s) 158, 939, 1745
Sfr274I CTCGAG 1 cut(s) 863
SfuI TTCGAA 1 cut(s) 228
SinI GGWCC 2 cut(s) 791, 1290
SlaI CTCGAG 1 cut(s) 863
SmlI CTYRAG 2 cut(s) 863, 1690
SmoI CTYRAG 2 cut(s) 863, 1690
SphI GCATGC 1 cut(s) 49
Sse9I AATT 5 cut(s) 561, 1103, 1483, 1544, 1634
SsiI CCGC 8 cut(s) 68, 90, 213, 553, 1430, 1505, 1775, 1815
SspI AATATT 1 cut(s) 1333
SspMI CTAG 2 cut(s) 146, 750
StyD4I CCNGG 2 cut(s) 848, 1800
StyI CCWWGG 2 cut(s) 826, 1286
TaaI ACNGT 4 cut(s) 108, 162, 321, 1918
TaiI ACGT 2 cut(s) 98, 117
TaqI TCGA 6 cut(s) 192, 228, 619, 864, 1120, 1514
TaqII GACCGA 1 cut(s) 705
TasI AATT 5 cut(s) 561, 1103, 1483, 1544, 1634
TatI WGTACW 2 cut(s) 290, 1225
TauI GCSGC 4 cut(s) 93, 555, 1432, 1508
TfiI GAWTC 8 cut(s) 77, 230, 259, 391, 397, 583, 845, 1871
Tru1I TTAA 5 cut(s) 500, 1088, 1239, 1344, 1548
Tru9I TTAA 5 cut(s) 500, 1088, 1239, 1344, 1548
TscAI CASTG 6 cut(s) 167, 881, 1027, 1300, 1458, 1912
TseFI GTSAC 4 cut(s) 8, 469, 1268, 1585
TseI GCWGC 8 cut(s) 254, 555, 611, 644, 647, 746, 1082, 1098
Tsp45I GTSAC 4 cut(s) 8, 469, 1268, 1585
TspDTI ATGAA 7 cut(s) 369, 659, 664, 1413, 1439, 1845, 1851
TspGWI ACGGA 1 cut(s) 1693
TspRI CASTG 6 cut(s) 167, 881, 1027, 1300, 1458, 1912
Tth111I GACNNNGTC 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 791, 1290
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 3 cut(s) 1483, 1544, 1634
XceI RCATGY 2 cut(s) 49, 899
XhoI CTCGAG 1 cut(s) 863
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 2 cut(s) 146, 750
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.