Prupe.5G140900_v2.0.a1

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000644
Physical Location & Seq
Forward (+)
13125563 .. 13131423
5861 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G140900.2

Sequence Viewer

Length: 2562 bp
ATGGCGTACGTTAGCCATCTGCCGTGCGATGGCGACGGCGTCTGCATGCTCTGCAAGACGAAACCGTCGGACGAGGACAAGCTCACCTGCAAGACCTGCGTCACGCCCTGGCACGTGGCCTGCCTCGTGACCCGACCCGAAAGCCTGGCATCCACTCTCCAGTGGGAGTGCCCAGACTGCACGACGTTCTCCGGCGAATCTCCGGCCGCACCCGCCGTGAACTCCGCCGGCTCCGGCGAGTTGGTCGCGGCGATCCGGGCGATCGAAAATGACGAGTCGTTGACGGAGCAGGAGAAGGCGAAGAAGCGACAGGAGCTGTTGAGCGGCAAAGCTGAAGTGGACGAAGACGACGGAGGTCGTCAGAAGGGAGGGAGGGACGTCTTGGATGTTCTGGATAAAATTTTCTACTGTTGCTTCTGCATGCAGCTACCGGAGAGACCTGTTACGACACCATGTGGACACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCAATGCCATTCCTCCCAAAATGGCAAGTCAACCCCGCATCAACTCTTCACTTGTAGTTGCCATCCGTATGGCTAAGGTGTCAAAATCAAATGTTACTGGGGGTCCTGCTAAGATTTATCAGTTTATACAAAACCAAGACAGGCCTGACAAAGCCTACACTACAGACCGGGCTCAGAAGACTGGAAAAGCAAATGCTGCCAGTGGCAAGATATTTGTCACTATTCCATCAGATCACTTTGGACCTATTCCTGCTGAAAATGATCCAGAGAGAAATCGAGGTGTGCTGGTTGGGGAAAGTTGGGAGGATAGGCTGGAATGTAGACAATGGGGTGTACACTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAATCATGGTGCACAGTCAGTTGTGCTTTCAGGTGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACTGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGAACAATCTTTTGACCAGACATTTGACAAATATAACAAGGCTCTCCAAGTTAGTTGCTTGAAAGGTTATCCTGTTCGAGTGGTAAGGTCTCACAAGGAGAAGCGCTCCTCTTATGCCCCTGAGAAAGGTGTGCGGTATGATGGATGCTACAGAATAGAAAAATGTTGGAAAAAGGTTGGAGTTCAGGGGTTCAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAATGAACCTGCACCATGGACGAGTGATGAACATGGGGATCGACCTAGACCCTTACCTCCTATTCCTGAGCTGAAGCAAGCCACAGATATAACAGAGAGGAAGCAAAGTCCATCTTGGGACTTTGTTGAGAAAGATGGCTGCTGGACATGGAAAAAGCCAGCGCCTGTCAGCAAAAAGCCTGTTCAAGCATTAAATCCTGACGATCGAAAGAGGTCAAGGAAAGCAATCAGGAAAGCACACAACACTACTGTCCGAGATAGGCTCTTGAAAGAATTCAGCTGCCAAATTTGCCGGCAGGTGATGACTTCACCAATAACCACACCTTGTGCTCATAACTTTTGCAAGTTGTGTTTAGAAGGTGCTTTTGCTGGTAAGAGTTTTGTCAGAGAAAGAAACAGAGGTGGGCGGTCTCTTCGATCTCAAAAGAATGTCATGAACTGCCCTTCTTGTCCCACAGACATCTCTGATTTTCTTCAAAATCCTCAGGTGAATAGAGAGATAATGGATATGATTGAGTCTCTAAAATCTACAAATGAAGAGGAGCCGAATGTGGATCCACCTGAGGAGTCGAGTGAAGAAGGAATGGCTGATTCCGACAATGAAGCTAGCACTCAGATGTCTGAAGGAAAAGTCGTTAATGATGGCGGGCAGAATTCTCCTTCAACCCACAAGTCCACAAGGAGCCCCAAACGTGTGAAATTTGATCTTGGTGATTGCTCAACAAAGACGGATGATGGGGAGGAAAATGAAATTCCTGCTGTCCAGGCTGATGGTTTGGAGGGAGATTCTGAGCTGGGAAATCCTAAAGAGAGCAACAAGTCAGTGGCTCCAGTTGCAAAGCATGGGAGGAAAGCTGTTGGTCAGGAGGCCCTGGAAGAGGAAGCAATGGGAGATGATGAAGGAAATGGTTCACCATCGAGTCCTCTGCAGGTGCAATCTGATGAGGATCCAAAAGAGAGCAATAAGTCGGAGGCTCCAGTTGCCAAGTGCGGGAGGAAAGCTGCTGGTCAGGCTGGTGTTGGAGCAAAGACCAGAGGAAGGAAGAAGGCTCCAGAGGAGGAAGCATTGGAAGATGATGAAGGAAATGGTTCACCATCAAGTCCACTGCAAGTGAAATCTGTTGAGGATGCAAAGGAGAGCAAAAAGTCAGTGGCTCCGGTTGCAAAGCGTGAGAAGAAAGCTGCGGTTCAAGGCGGCGTTGCAGCGAGGACCCGAGGGAGGAAGAAGGCTCAGGAGGAGGAAGCATTGGGTGATGATGAAGGAAATGTTTCACCATCAAGTCCTCTGCATGTGAAAACTGACGATGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

854

Amino Acids

93.56

Weight (kDa)

6.62

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 95, 1531, 2164
AasI GACNNNNNNGTC 1 cut(s) 64
AatII GACGTC 1 cut(s) 381
Acc36I ACCTGC 5 cut(s) 95, 104, 1261, 1531, 2164
Acc65I GGTACC 1 cut(s) 933
AccB1I GGYRCC 1 cut(s) 933
AccBSI CCGCTC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 847
AccII CGCG 1 cut(s) 248
AclWI GGATC 8 cut(s) 247, 782, 957, 1290, 1793, 1806, 2186, 2199
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 6 cut(s) 399, 1517, 1530, 1897, 1943, 1995
AcuI CTGAAG 4 cut(s) 354, 494, 1337, 1887
AcvI CACGTG 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 39, 378
AdeI CACNNNGTG 2 cut(s) 455, 1571
AfaI GTAC 4 cut(s) 8, 511, 861, 935
AfeI AGCGCT 1 cut(s) 1124
AfiI CCNNNNNNNGG 7 cut(s) 29, 490, 1145, 2122, 2235, 2283, 2463
AflIII ACRYGT 1 cut(s) 1936
AgsI TTSAA 8 cut(s) 1081, 1213, 1430, 1513, 1721, 1908, 2435, 2558
AjnI CCWGG 4 cut(s) 107, 144, 2007, 2115
Alw21I GWGCWC 2 cut(s) 910, 1576
Alw26I GTCTC 4 cut(s) 430, 1113, 1659, 1767
Alw44I GTGCAC 1 cut(s) 906
AlwI GGATC 8 cut(s) 247, 782, 957, 1290, 1793, 1806, 2186, 2199
AlwNI CAGNNNCTG 2 cut(s) 316, 1409
Ama87I CYCGRG 1 cut(s) 2457
Aor51HI AGCGCT 1 cut(s) 1124
AoxI GGCC 4 cut(s) 117, 204, 668, 2112
ApaLI GTGCAC 1 cut(s) 906
ApeKI GCWGC 7 cut(s) 424, 722, 1383, 1524, 2246, 2426, 2447
ApoI RAATTY 6 cut(s) 399, 1517, 1530, 1897, 1943, 1995
ArsI GACNNNNNNTTYG 3 cut(s) 1025, 1057, 2537
Asp700I GAANNNNTTC 5 cut(s) 479, 1517, 2152, 2332, 2512
Asp718I GGTACC 1 cut(s) 933
AspLEI GCGC 2 cut(s) 1125, 1408
AspS9I GGNCC 4 cut(s) 629, 767, 2113, 2454
AsuC2I CCSGG 2 cut(s) 257, 695
AsuHPI GGTGA 9 cut(s) 76, 1545, 1555, 1744, 1967, 2148, 2328, 2507, 2508
AsuNHI GCTAGC 1 cut(s) 1850
AvaI CYCGRG 1 cut(s) 2457
AvaII GGWCC 3 cut(s) 629, 767, 2454
AxyI CCTNAGG 2 cut(s) 1728, 1806
BaeGI GKGCMC 2 cut(s) 173, 910
BamHI GGATCC 2 cut(s) 1798, 2191
BanI GGYRCC 1 cut(s) 933
BanII GRGCYC 2 cut(s) 700, 1931
BauI CACGAG 1 cut(s) 125
BbrPI CACGTG 1 cut(s) 115
BbsI GAAGAC 2 cut(s) 351, 710
Bbv12I GWGCWC 2 cut(s) 910, 1576
BbvI GCAGC 7 cut(s) 436, 709, 1370, 1511, 2233, 2413, 2459
BceAI ACGGC 3 cut(s) 7, 52, 200
BcgI CGANNNNNNTGC 2 cut(s) 2152, 2186
BciT130I CCWGG 4 cut(s) 109, 146, 2009, 2117
BcnI CCSGG 2 cut(s) 257, 695
BcoDI GTCTC 4 cut(s) 430, 1113, 1659, 1767
BfaI CTAG 2 cut(s) 1290, 1851
BfmI CTRYAG 3 cut(s) 687, 1168, 2171
BfoI RGCGCY 2 cut(s) 1126, 1409
BfuAI ACCTGC 5 cut(s) 95, 104, 1261, 1531, 2164
Bme1390I CCNGG 6 cut(s) 109, 146, 257, 695, 2009, 2117
Bme18I GGWCC 3 cut(s) 629, 767, 2454
BmeT110I CYCGRG 1 cut(s) 2457
BmgT120I GGNCC 4 cut(s) 629, 767, 2113, 2454
BmrFI CCNGG 6 cut(s) 109, 146, 257, 695, 2009, 2117
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 4 cut(s) 158, 573, 1154, 2362
BmtI GCTAGC 1 cut(s) 1854
BmuI ACTGGG 1 cut(s) 633
BoxI GACNNNNGTC 2 cut(s) 98, 354
BpiI GAAGAC 2 cut(s) 351, 710
BplI GAGNNNNNCTC 4 cut(s) 1109, 1141, 1491, 1523
BpmI CTGGAG 4 cut(s) 143, 2058, 2205, 2280
Bpu10I CCTNAGC 3 cut(s) 600, 1311, 2475
BpuMI CCSGG 2 cut(s) 257, 695
BsaAI YACGTR 1 cut(s) 115
BsaBI GATNNNNATC 2 cut(s) 948, 2190
BsaHI GRCGYC 2 cut(s) 39, 378
BsaI GGTCTC 3 cut(s) 430, 1113, 1659
BsaJI CCNNGG 4 cut(s) 107, 1259, 2115, 2458
BsaWI WCCGGW 2 cut(s) 430, 2401
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 490, 1145, 2122, 2235, 2283, 2463
Bse118I RCCGGY 3 cut(s) 227, 1221, 1536
Bse1I ACTGG 7 cut(s) 160, 628, 712, 726, 979, 2075, 2222
Bse21I CCTNAGG 2 cut(s) 1728, 1806
Bse3DI GCAATG 2 cut(s) 535, 2136
Bse8I GATNNNNATC 2 cut(s) 948, 2190
BseBI CCWGG 4 cut(s) 109, 146, 2009, 2117
BseDI CCNNGG 4 cut(s) 107, 1259, 2115, 2458
BseGI GGATG 6 cut(s) 149, 391, 588, 1169, 1981, 2377
BseJI GATNNNNATC 2 cut(s) 948, 2190
BseLI CCNNNNNNNGG 7 cut(s) 29, 490, 1145, 2122, 2235, 2283, 2463
BseMI GCAATG 2 cut(s) 535, 2136
BseMII CTCAG 8 cut(s) 713, 1131, 1302, 1742, 1797, 1871, 2025, 2489
BseNI ACTGG 7 cut(s) 160, 628, 712, 726, 979, 2075, 2222
BseRI GAGGAG 5 cut(s) 1117, 1799, 1823, 2315, 2495
BseSI GKGCMC 2 cut(s) 173, 910
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 7 cut(s) 436, 709, 1370, 1511, 2233, 2413, 2459
BseYI CCCAGC 1 cut(s) 2038
BsgI GTGCAG 2 cut(s) 163, 1239
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 3 cut(s) 207, 264, 1450
BshFI GGCC 4 cut(s) 119, 206, 670, 2114
BshNI GGYRCC 1 cut(s) 933
BsiEI CGRYCG 3 cut(s) 207, 264, 1450
BsiHKAI GWGCWC 2 cut(s) 910, 1576
BsiHKCI CYCGRG 1 cut(s) 2457
BsiWI CGTACG 1 cut(s) 6
BslFI GGGAC 3 cut(s) 389, 1376, 1680
BslI CCNNNNNNNGG 7 cut(s) 29, 490, 1145, 2122, 2235, 2283, 2463
BsmAI GTCTC 4 cut(s) 430, 1113, 1659, 1767
BsmFI GGGAC 3 cut(s) 389, 1376, 1680
BsnI GGCC 4 cut(s) 119, 206, 670, 2114
Bso31I GGTCTC 3 cut(s) 430, 1113, 1659
BsoBI CYCGRG 1 cut(s) 2457
Bsp1286I GDGCHC 5 cut(s) 173, 700, 910, 1576, 1931
Bsp1407I TGTACA 1 cut(s) 859
Bsp19I CCATGG 1 cut(s) 1259
BspANI GGCC 4 cut(s) 119, 206, 670, 2114
BspCNI CTCAG 8 cut(s) 712, 1132, 1303, 1741, 1798, 1870, 2026, 2488
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 1677
BspMAI CTGCAG 1 cut(s) 2175
BspMI ACCTGC 5 cut(s) 95, 104, 1261, 1531, 2164
BspOI GCTAGC 1 cut(s) 1854
BspPI GGATC 8 cut(s) 247, 782, 957, 1290, 1793, 1806, 2186, 2199
BspT107I GGYRCC 1 cut(s) 933
BspTNI GGTCTC 3 cut(s) 430, 1113, 1659
BsrBI CCGCTC 1 cut(s) 324
BsrDI GCAATG 2 cut(s) 535, 2136
BsrFI RCCGGY 3 cut(s) 227, 1221, 1536
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 7 cut(s) 160, 628, 712, 726, 979, 2075, 2222
BssAI RCCGGY 3 cut(s) 227, 1221, 1536
BssECI CCNNGG 4 cut(s) 107, 1259, 2115, 2458
BssNI GRCGYC 2 cut(s) 39, 378
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 1259
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 4 cut(s) 109, 146, 2009, 2117
Bst4CI ACNGT 5 cut(s) 66, 410, 894, 912, 1495
Bst6I CTCTTC 4 cut(s) 577, 1662, 1776, 2115
BstACI GRCGYC 2 cut(s) 39, 378
BstAPI GCANNNNNTGC 3 cut(s) 51, 96, 722
BstAUI TGTACA 1 cut(s) 859
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 9 cut(s) 47, 121, 229, 422, 1323, 1404, 1538, 1852, 1892
BstDSI CCRYGG 1 cut(s) 1259
BstENI CCTNNNNNAGG 2 cut(s) 1143, 2120
BstF5I GGATG 6 cut(s) 149, 391, 588, 1169, 1981, 2377
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 2 cut(s) 1126, 1409
BstHHI GCGC 2 cut(s) 1125, 1408
BstMAI GTCTC 4 cut(s) 430, 1113, 1659, 1767
BstMCI CGRYCG 3 cut(s) 207, 264, 1450
BstNI CCWGG 4 cut(s) 109, 146, 2009, 2117
BstNSI RCATGY 3 cut(s) 49, 424, 2537
BstPAI GACNNNNGTC 2 cut(s) 98, 354
BstSCI CCNGG 6 cut(s) 107, 144, 255, 693, 2007, 2115
BstSFI CTRYAG 3 cut(s) 687, 1168, 2171
BstSLI GKGCMC 2 cut(s) 173, 910
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 7 cut(s) 436, 709, 1370, 1511, 2233, 2413, 2459
BstV2I GAAGAC 2 cut(s) 351, 710
BstX2I RGATCY 2 cut(s) 1798, 2191
BstXI CCANNNNNNTGG 3 cut(s) 595, 878, 2015
BstYI RGATCY 2 cut(s) 1798, 2191
BstZI CGGCCG 1 cut(s) 204
Bsu36I CCTNAGG 2 cut(s) 1728, 1806
BsuRI GGCC 4 cut(s) 119, 206, 670, 2114
BtgI CCRYGG 1 cut(s) 1259
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 6 cut(s) 149, 391, 588, 1169, 1981, 2377
BtsI GCAGTG 1 cut(s) 2348
BtsIMutI CAGTG 6 cut(s) 167, 733, 972, 2073, 2348, 2400
BveI ACCTGC 5 cut(s) 95, 104, 1261, 1531, 2164
Cac8I GCNNGC 9 cut(s) 47, 121, 229, 422, 1323, 1404, 1538, 1852, 1892
CaiI CAGNNNCTG 2 cut(s) 316, 1409
CciI TCATGA 1 cut(s) 1677
CfoI GCGC 2 cut(s) 1125, 1408
Cfr10I RCCGGY 3 cut(s) 227, 1221, 1536
Cfr13I GGNCC 4 cut(s) 629, 767, 2113, 2454
CseI GACGC 2 cut(s) 28, 88
Csp6I GTAC 4 cut(s) 7, 510, 860, 934
CviQI GTAC 4 cut(s) 7, 510, 860, 934
DraIII CACNNNGTG 2 cut(s) 455, 1571
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 204
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 577, 1662, 1776, 2115
EarI CTCTTC 4 cut(s) 577, 1662, 1776, 2115
EciI GGCGGA 1 cut(s) 214
EclXI CGGCCG 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 1259
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 2 cut(s) 700, 1931
Eco31I GGTCTC 3 cut(s) 430, 1113, 1659
Eco47I GGWCC 3 cut(s) 629, 767, 2454
Eco47III AGCGCT 1 cut(s) 1124
Eco52I CGGCCG 1 cut(s) 204
Eco57I CTGAAG 4 cut(s) 354, 494, 1337, 1887
Eco72I CACGTG 1 cut(s) 115
Eco81I CCTNAGG 2 cut(s) 1728, 1806
Eco88I CYCGRG 1 cut(s) 2457
EcoNI CCTNNNNNAGG 2 cut(s) 1143, 2120
EcoO109I RGGNCCY 3 cut(s) 629, 2113, 2454
EcoRI GAATTC 2 cut(s) 1517, 1897
EcoRII CCWGG 4 cut(s) 107, 144, 2007, 2115
EcoT14I CCWWGG 1 cut(s) 1259
EcoT38I GRGCYC 2 cut(s) 700, 1931
ErhI CCWWGG 1 cut(s) 1259
FalI AAGNNNNNCTT 2 cut(s) 1342, 1374
FaqI GGGAC 3 cut(s) 389, 1376, 1680
FauI CCCGC 4 cut(s) 220, 569, 1883, 2228
FblI GTMKAC 1 cut(s) 847
FokI GGATG 6 cut(s) 136, 398, 575, 1176, 1988, 2384
FriOI GRGCYC 2 cut(s) 700, 1931
FspBI CTAG 2 cut(s) 1290, 1851
GlaI GCGC 2 cut(s) 1124, 1407
GsaI CCCAGC 1 cut(s) 2042
GsuI CTGGAG 4 cut(s) 143, 2058, 2205, 2280
HaeII RGCGCY 2 cut(s) 1126, 1409
HaeIII GGCC 4 cut(s) 119, 206, 670, 2114
HgaI GACGC 2 cut(s) 28, 88
HhaI GCGC 2 cut(s) 1125, 1408
Hin1I GRCGYC 2 cut(s) 39, 378
Hin6I GCGC 2 cut(s) 1123, 1406
HinP1I GCGC 2 cut(s) 1123, 1406
HincII GTYRAC 2 cut(s) 282, 557
HindII GTYRAC 2 cut(s) 282, 557
HinfI GANTC 7 cut(s) 197, 275, 1760, 1811, 1835, 2030, 2164
HphI GGTGA 9 cut(s) 76, 1545, 1555, 1744, 1967, 2148, 2328, 2507, 2508
Hpy99I CGWCG 4 cut(s) 38, 70, 187, 353
HpyCH4III ACNGT 5 cut(s) 66, 410, 894, 912, 1495
HpyCH4IV ACGT 5 cut(s) 9, 114, 185, 378, 1936
HpySE526I ACGT 5 cut(s) 9, 114, 185, 378, 1936
Hsp92I GRCGYC 2 cut(s) 39, 378
HspAI GCGC 2 cut(s) 1123, 1406
KpnI GGTACC 1 cut(s) 937
KroI GCCGGC 2 cut(s) 227, 1536
KroNI GCCGGC 2 cut(s) 229, 1538
Lsp1109I GCAGC 7 cut(s) 436, 709, 1370, 1511, 2233, 2413, 2459
LweI GCATC 4 cut(s) 158, 573, 1154, 2362
MaeI CTAG 2 cut(s) 1290, 1851
MaeII ACGT 5 cut(s) 9, 114, 185, 378, 1936
MaeIII GTNAC 6 cut(s) 100, 127, 442, 619, 742, 1241
MbiI CCGCTC 1 cut(s) 324
MflI RGATCY 2 cut(s) 1798, 2191
MhlI GDGCHC 5 cut(s) 173, 700, 910, 1576, 1931
MluCI AATT 7 cut(s) 399, 463, 1517, 1530, 1897, 1943, 1995
MlyI GAGTC 4 cut(s) 284, 1769, 1820, 2173
MmeI TCCRAC 7 cut(s) 48, 475, 1166, 1177, 1863, 2193, 2245
MroNI GCCGGC 2 cut(s) 227, 1536
MroXI GAANNNNTTC 5 cut(s) 479, 1517, 2152, 2332, 2512
MseI TTAA 2 cut(s) 1436, 1881
MslI CAYNNNNRTG 2 cut(s) 593, 1859
MspA1I CMGCKG 1 cut(s) 1524
MspR9I CCNGG 6 cut(s) 109, 146, 257, 695, 2009, 2117
MvaI CCWGG 4 cut(s) 109, 146, 2009, 2117
MvnI CGCG 1 cut(s) 248
NaeI GCCGGC 2 cut(s) 229, 1538
NciI CCSGG 2 cut(s) 257, 695
NcoI CCATGG 1 cut(s) 1259
NgoMIV GCCGGC 2 cut(s) 227, 1536
NheI GCTAGC 1 cut(s) 1850
NmuCI GTSAC 4 cut(s) 100, 127, 742, 1241
NspI RCATGY 3 cut(s) 49, 424, 2537
PaeI GCATGC 2 cut(s) 49, 424
PagI TCATGA 1 cut(s) 1677
PaqCI CACCTGC 3 cut(s) 95, 1531, 2164
PceI AGGCCT 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 2 cut(s) 229, 1538
PdmI GAANNNNTTC 5 cut(s) 479, 1517, 2152, 2332, 2512
PfeI GAWTC 3 cut(s) 197, 1835, 2030
Pfl23II CGTACG 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 38
Ple19I CGATCG 2 cut(s) 264, 1450
PleI GAGTC 4 cut(s) 283, 1768, 1819, 2172
PmaCI CACGTG 1 cut(s) 115
PmlI CACGTG 1 cut(s) 115
PpsI GAGTC 4 cut(s) 283, 1768, 1819, 2172
Ppu21I YACGTR 1 cut(s) 115
PpuMI RGGWCCY 2 cut(s) 629, 2454
PshAI GACNNNNGTC 2 cut(s) 98, 354
Psp5II RGGWCCY 2 cut(s) 629, 2454
Psp6I CCWGG 4 cut(s) 107, 144, 2007, 2115
PspCI CACGTG 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 2038
PspGI CCWGG 4 cut(s) 107, 144, 2007, 2115
PspLI CGTACG 1 cut(s) 6
PspPI GGNCC 4 cut(s) 629, 767, 2113, 2454
PspPPI RGGWCCY 2 cut(s) 629, 2454
PstI CTGCAG 1 cut(s) 2175
PstNI CAGNNNCTG 2 cut(s) 316, 1409
PsuI RGATCY 2 cut(s) 1798, 2191
PsyI GACNNNGTC 1 cut(s) 38
PvuI CGATCG 2 cut(s) 264, 1450
PvuII CAGCTG 1 cut(s) 1524
RsaI GTAC 4 cut(s) 8, 511, 861, 935
RsaNI GTAC 4 cut(s) 7, 510, 860, 934
RseI CAYNNNNRTG 2 cut(s) 593, 1859
SaqAI TTAA 2 cut(s) 1436, 1881
Sau96I GGNCC 4 cut(s) 629, 767, 2113, 2454
SchI GAGTC 4 cut(s) 284, 1769, 1820, 2173
ScrFI CCNGG 6 cut(s) 109, 146, 257, 695, 2009, 2117
SduI GDGCHC 5 cut(s) 173, 700, 910, 1576, 1931
SfaNI GCATC 4 cut(s) 158, 573, 1154, 2362
SfcI CTRYAG 3 cut(s) 687, 1168, 2171
SinI GGWCC 3 cut(s) 629, 767, 2454
SmiMI CAYNNNNRTG 2 cut(s) 593, 1859
SphI GCATGC 2 cut(s) 49, 424
Sse9I AATT 7 cut(s) 399, 463, 1517, 1530, 1897, 1943, 1995
SseBI AGGCCT 1 cut(s) 670
SspMI CTAG 2 cut(s) 1290, 1851
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 6 cut(s) 107, 144, 255, 693, 2007, 2115
StyI CCWWGG 1 cut(s) 1259
TaaI ACNGT 5 cut(s) 66, 410, 894, 912, 1495
TaiI ACGT 5 cut(s) 12, 117, 188, 381, 1939
TaqI TCGA 8 cut(s) 264, 802, 1096, 1285, 1450, 1660, 1814, 2162
TasI AATT 7 cut(s) 399, 463, 1517, 1530, 1897, 1943, 1995
TatI WGTACW 1 cut(s) 859
TauI GCSGC 5 cut(s) 209, 251, 327, 528, 2442
TfiI GAWTC 3 cut(s) 197, 1835, 2030
Tru1I TTAA 2 cut(s) 1436, 1881
Tru9I TTAA 2 cut(s) 1436, 1881
TscAI CASTG 6 cut(s) 167, 733, 979, 2073, 2355, 2400
TseFI GTSAC 4 cut(s) 100, 127, 742, 1241
TseI GCWGC 7 cut(s) 424, 722, 1383, 1524, 2246, 2426, 2447
Tsp45I GTSAC 4 cut(s) 100, 127, 742, 1241
TspDTI ATGAA 9 cut(s) 1263, 1287, 1694, 1794, 1860, 2007, 2157, 2337, 2517
TspGWI ACGGA 4 cut(s) 299, 366, 581, 1988
TspRI CASTG 6 cut(s) 167, 733, 979, 2073, 2355, 2400
Tth111I GACNNNGTC 1 cut(s) 38
VneI GTGCAC 1 cut(s) 906
VpaK11BI GGWCC 3 cut(s) 629, 767, 2454
XagI CCTNNNNNAGG 2 cut(s) 1143, 2120
XapI RAATTY 6 cut(s) 399, 1517, 1530, 1897, 1943, 1995
XceI RCATGY 3 cut(s) 49, 424, 2537
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 847
XmnI GAANNNNTTC 5 cut(s) 479, 1517, 2152, 2332, 2512
XspI CTAG 2 cut(s) 1290, 1851
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.