Rorug06G0491600

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000644
Physical Location & Seq
Reverse (-)
60976504 .. 60979638
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0491600.1

Sequence Viewer

Length: 1026 bp
ATGGCTTCTTCACCTTCTCCCTACCAGACACAGGTTCCTCTTTCGCCGGACCCAGGTGAAATACCCGTAATTCATGAAACCCAGTTCCCATGGGTGCCTGTTCAGATAGTGACTCATCCATCTCAGCTTCCTGCTGAGTTTCTGGAGCCTTCTGCTGACAGGCCATTGGTGATTGGGTTTGACTGCGAGGGAGTTGACCTGTGTCGAAATGGAACACTCTGTATCATGCAGCTTGCATTTCCAGAGGCTATATATCTGGTGGATGCCATTCAGGGAGGGGAGATGCTTATACAAGCCTGTAAGCCTGCACTTGAGTCTAGCCACGTCACAAAAGTTATTCATGACTGCAAGCGAGACAGTGAGGCCTTGTATTTTCAGTTCGGCATCAAGTTGAACAATGTTGTAGATACCCAGATTGCTTATTCGTTGATAGAAGAGCAAGAAGGTCGGAAAAGATTACCAGATGATTATATATCATTCGTTGGCCTCCTTGCTGATCCACGTTATTGTGGAGTATCTTATCTCGAGAAGGAAGAAGTCCGTTTTCTTCTTAGGCAGGACCCCAACTTTTGGACGTATAGACCTTTATCTGAACAGATGGTTCGTGCAGCTGCAGATGATGTTCGCTTTCTTCCATATATCTATTGCAGGATGATGGGGAAATTGAATCAACAGTCATTATGGTATCTTGCTGTTCGTGGTGCACTTTATTGTCGATGTTTTTGCATCACCAATAATGACTTTTCTGACTGGCCACCTCTCCCTACCATTCCAGATAACCTACTAGTGGAGGGCAGTGCGCCCGAGGAAGAAATCCTTTCGGTTCTTGATGTTCCCCCAGGAAAAATGGGACGTGTAATTGGGAAAAGAGGGACTTCCATTCTGTCAATAAAAGAATCCTGCGATGCGGAAATCCTCATGGGAGGTGATAAGGGCCCACCAGACAAGGTGTTCATCATAGGACCAGTGAAGCAGGTGAGGAAAGCAGAAGCCATGTTAAGGGGAAGGATGCTGGACATGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

341

Amino Acids

38.32

Weight (kDa)

4.93

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 41 - 222 1.9e-22 3'-5' exonuclease
KH_1 PF00013 276 - 334 6.3e-10 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 570
AciI CCGC 1 cut(s) 908
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 752
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 5 cut(s) 31, 53, 570, 787, 999
AflIII ACRYGT 2 cut(s) 853, 1017
AgsI TTSAA 2 cut(s) 394, 667
AhlI ACTAGT 1 cut(s) 784
AjiI CACGTC 2 cut(s) 325, 854
AjnI CCWGG 2 cut(s) 52, 838
AluBI AGCT 3 cut(s) 127, 232, 611
AluI AGCT 3 cut(s) 127, 232, 611
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 1 cut(s) 348
Alw44I GTGCAC 1 cut(s) 702
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 2 cut(s) 524, 803
AoxI GGCC 5 cut(s) 161, 363, 484, 752, 934
ApaI GGGCCC 1 cut(s) 938
ApaLI GTGCAC 1 cut(s) 702
ApeKI GCWGC 3 cut(s) 229, 608, 611
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 5 cut(s) 49, 559, 934, 935, 962
AsuHPI GGTGA 6 cut(s) 3, 68, 181, 721, 938, 988
AvaI CYCGRG 2 cut(s) 524, 803
AvaII GGWCC 3 cut(s) 49, 559, 962
BaeGI GKGCMC 2 cut(s) 706, 938
BalI TGGCCA 1 cut(s) 754
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 3 cut(s) 241, 598, 620
BccI CCATC 3 cut(s) 127, 592, 649
BcgI CGANNNNNNTGC 4 cut(s) 428, 462, 705, 739
BciT130I CCWGG 2 cut(s) 54, 840
BcoDI GTCTC 1 cut(s) 348
BcuI ACTAGT 1 cut(s) 784
BfaI CTAG 2 cut(s) 318, 785
BfmI CTRYAG 1 cut(s) 612
BfuAI ACCTGC 1 cut(s) 964
BisI GCNGC 3 cut(s) 230, 609, 612
BlsI GCNGC 3 cut(s) 231, 610, 613
Bme1390I CCNGG 2 cut(s) 54, 840
Bme18I GGWCC 3 cut(s) 49, 559, 962
BmeT110I CYCGRG 2 cut(s) 524, 803
BmgBI CACGTC 2 cut(s) 325, 854
BmgT120I GGNCC 5 cut(s) 49, 559, 934, 935, 962
BmiI GGNNCC 6 cut(s) 36, 51, 96, 147, 561, 936
BmrFI CCNGG 2 cut(s) 54, 840
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 6 cut(s) 253, 273, 393, 735, 895, 999
BmuI ACTGGG 1 cut(s) 76
BoxI GACNNNNGTC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 52, 89, 804, 838
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 53, 570, 787, 999
Bse1I ACTGG 3 cut(s) 82, 755, 965
BseBI CCWGG 2 cut(s) 54, 840
BseDI CCNNGG 4 cut(s) 52, 89, 804, 838
BseGI GGATG 4 cut(s) 115, 268, 657, 1014
BseLI CCNNNNNNNGG 5 cut(s) 31, 53, 570, 787, 999
BseMII CTCAG 2 cut(s) 126, 137
BseNI ACTGG 3 cut(s) 82, 755, 965
BseSI GKGCMC 2 cut(s) 706, 938
BseXI GCAGC 3 cut(s) 241, 598, 620
BsgI GTGCAG 2 cut(s) 291, 627
BshFI GGCC 5 cut(s) 163, 365, 486, 754, 936
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 706
BsiHKCI CYCGRG 2 cut(s) 524, 803
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 864, 886
BslI CCNNNNNNNGG 5 cut(s) 31, 53, 570, 787, 999
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 2 cut(s) 864, 886
BsnI GGCC 5 cut(s) 163, 365, 486, 754, 936
BsoBI CYCGRG 2 cut(s) 524, 803
Bsp120I GGGCCC 1 cut(s) 934
Bsp1286I GDGCHC 2 cut(s) 706, 938
Bsp143I GATC 1 cut(s) 496
Bsp19I CCATGG 1 cut(s) 89
BspACI CCGC 1 cut(s) 908
BspANI GGCC 5 cut(s) 163, 365, 486, 754, 936
BspCNI CTCAG 2 cut(s) 127, 136
BspHI TCATGA 2 cut(s) 73, 340
BspLI GGNNCC 6 cut(s) 36, 51, 96, 147, 561, 936
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 94
BsrI ACTGG 3 cut(s) 82, 755, 965
BssECI CCNNGG 4 cut(s) 52, 89, 804, 838
BssMI GATC 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 2 cut(s) 54, 840
Bst4CI ACNGT 2 cut(s) 359, 675
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 234, 306, 350
BstDEI CTNAG 3 cut(s) 123, 135, 551
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 4 cut(s) 115, 268, 657, 1014
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 1 cut(s) 496
BstNI CCWGG 2 cut(s) 54, 840
BstNSI RCATGY 1 cut(s) 1021
BstPAI GACNNNNGTC 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 52, 838
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 2 cut(s) 706, 938
BstV1I GCAGC 3 cut(s) 241, 598, 620
BsuRI GGCC 5 cut(s) 163, 365, 486, 754, 936
BtgI CCRYGG 1 cut(s) 89
BtgZI GCGATG 1 cut(s) 918
BtrI CACGTC 2 cut(s) 325, 854
BtsCI GGATG 4 cut(s) 115, 268, 657, 1014
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 3 cut(s) 364, 802, 972
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 3 cut(s) 234, 306, 350
CciI TCATGA 2 cut(s) 73, 340
CfoI GCGC 1 cut(s) 802
Cfr13I GGNCC 5 cut(s) 49, 559, 934, 935, 962
Csp6I GTAC 1 cut(s) 1021
CviAII CATG 7 cut(s) 74, 90, 226, 341, 919, 994, 1018
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 3 cut(s) 123, 135, 551
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 752
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 89
Eco147I AGGCCT 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 938
Eco47I GGWCC 3 cut(s) 49, 559, 962
Eco88I CYCGRG 2 cut(s) 524, 803
EcoO109I RGGNCCY 2 cut(s) 559, 934
EcoRII CCWGG 2 cut(s) 52, 838
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 938
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 7 cut(s) 77, 93, 229, 344, 922, 997, 1021
FalI AAGNNNNNCTT 4 cut(s) 801, 833, 859, 891
FaqI GGGAC 2 cut(s) 864, 886
FatI CATG 7 cut(s) 73, 89, 225, 340, 918, 993, 1017
Fnu4HI GCNGC 3 cut(s) 230, 609, 612
FokI GGATG 4 cut(s) 102, 275, 664, 1021
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 3 cut(s) 230, 609, 612
FspBI CTAG 2 cut(s) 318, 785
GlaI GCGC 1 cut(s) 801
GluI GCNGC 3 cut(s) 230, 609, 612
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 5 cut(s) 163, 365, 486, 754, 936
HapII CCGG 1 cut(s) 47
HhaI GCGC 1 cut(s) 802
Hin1II CATG 7 cut(s) 77, 93, 229, 344, 922, 997, 1021
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 4 cut(s) 112, 314, 667, 896
HpaII CCGG 1 cut(s) 47
HphI GGTGA 6 cut(s) 3, 68, 181, 721, 938, 988
Hpy166II GTNNAC 2 cut(s) 196, 704
Hpy188I TCNGA 4 cut(s) 105, 450, 592, 748
Hpy188III TCNNGA 8 cut(s) 74, 143, 242, 341, 524, 526, 773, 827
Hpy8I GTNNAC 2 cut(s) 196, 704
HpyAV CCTTC 5 cut(s) 24, 159, 437, 523, 999
HpyCH4III ACNGT 2 cut(s) 359, 675
HpyCH4IV ACGT 4 cut(s) 324, 502, 575, 853
HpyCH4V TGCA 9 cut(s) 229, 236, 308, 348, 608, 614, 648, 704, 726
HpyF3I CTNAG 3 cut(s) 123, 135, 551
HpySE526I ACGT 4 cut(s) 324, 502, 575, 853
Hsp92II CATG 7 cut(s) 77, 93, 229, 344, 922, 997, 1021
HspAI GCGC 1 cut(s) 800
Kzo9I GATC 1 cut(s) 496
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 3 cut(s) 241, 598, 620
LweI GCATC 6 cut(s) 253, 273, 393, 735, 895, 999
MaeI CTAG 2 cut(s) 318, 785
MaeII ACGT 4 cut(s) 324, 502, 575, 853
MaeIII GTNAC 2 cut(s) 109, 325
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 5 cut(s) 446, 539, 545, 623, 821
MhlI GDGCHC 2 cut(s) 706, 938
MlsI TGGCCA 1 cut(s) 754
MluCI AATT 3 cut(s) 69, 662, 858
MluNI TGGCCA 1 cut(s) 754
MlyI GAGTC 2 cut(s) 106, 323
MmeI TCCRAC 1 cut(s) 428
Mox20I TGGCCA 1 cut(s) 754
MscI TGGCCA 1 cut(s) 754
MseI TTAA 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 754
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 54, 840
MvaI CCWGG 2 cut(s) 54, 840
NcoI CCATGG 1 cut(s) 89
NdeII GATC 1 cut(s) 496
NlaIII CATG 7 cut(s) 77, 93, 229, 344, 922, 997, 1021
NlaIV GGNNCC 6 cut(s) 36, 51, 96, 147, 561, 936
NmuCI GTSAC 2 cut(s) 109, 325
NspI RCATGY 1 cut(s) 1021
PaeR7I CTCGAG 1 cut(s) 524
PagI TCATGA 2 cut(s) 73, 340
PaqCI CACCTGC 1 cut(s) 964
PceI AGGCCT 1 cut(s) 365
PciI ACATGT 1 cut(s) 1017
PciSI GCTCTTC 1 cut(s) 429
PfeI GAWTC 2 cut(s) 667, 896
PflMI CCANNNNNTGG 1 cut(s) 570
PkrI GCNGC 3 cut(s) 231, 610, 613
PleI GAGTC 2 cut(s) 106, 322
PpsI GAGTC 2 cut(s) 106, 322
PpuMI RGGWCCY 1 cut(s) 559
PscI ACATGT 1 cut(s) 1017
PshAI GACNNNNGTC 1 cut(s) 201
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 2 cut(s) 52, 838
PspGI CCWGG 2 cut(s) 52, 838
PspN4I GGNNCC 6 cut(s) 36, 51, 96, 147, 561, 936
PspOMI GGGCCC 1 cut(s) 934
PspPI GGNCC 5 cut(s) 49, 559, 934, 935, 962
PspPPI RGGWCCY 1 cut(s) 559
PsrI GAACNNNNNNTAC 2 cut(s) 362, 394
PstI CTGCAG 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 3 cut(s) 230, 609, 612
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 5 cut(s) 49, 559, 934, 935, 962
SchI GAGTC 2 cut(s) 106, 323
ScrFI CCNGG 2 cut(s) 54, 840
SduI GDGCHC 2 cut(s) 706, 938
SfaNI GCATC 6 cut(s) 253, 273, 393, 735, 895, 999
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 524
SinI GGWCC 3 cut(s) 49, 559, 962
SlaI CTCGAG 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 311, 524
SmoI CTYRAG 2 cut(s) 311, 524
SpeI ACTAGT 1 cut(s) 784
Sse9I AATT 3 cut(s) 69, 662, 858
SseBI AGGCCT 1 cut(s) 365
SsiI CCGC 1 cut(s) 908
SspMI CTAG 2 cut(s) 318, 785
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 2 cut(s) 52, 838
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 2 cut(s) 359, 675
TaiI ACGT 4 cut(s) 327, 505, 578, 856
TaqI TCGA 3 cut(s) 205, 525, 715
TasI AATT 3 cut(s) 69, 662, 858
TatI WGTACW 1 cut(s) 1020
TfiI GAWTC 2 cut(s) 667, 896
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 3 cut(s) 364, 802, 972
TseFI GTSAC 2 cut(s) 109, 325
TseI GCWGC 3 cut(s) 229, 608, 611
Tsp45I GTSAC 2 cut(s) 109, 325
TspDTI ATGAA 4 cut(s) 62, 90, 329, 943
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 3 cut(s) 364, 802, 972
Van91I CCANNNNNTGG 1 cut(s) 570
VneI GTGCAC 1 cut(s) 702
VpaK11BI GGWCC 3 cut(s) 49, 559, 962
XceI RCATGY 1 cut(s) 1021
XhoI CTCGAG 1 cut(s) 524
XspI CTAG 2 cut(s) 318, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.