RLG00000002800

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000644
Physical Location & Seq
Forward (+)
40063694 .. 40069619
5926 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002800

Sequence Viewer

Length: 2649 bp
ATGGCGCACGTTAGCCAACTGCCTTGCGACGGCGACGGCGTATGCATGCTCTGCAAGGCGAAGCCGTCCGACGAGGAGAAGCTGACCTGCAAGACCTGCGTCACGCCGTGGCACGCCACCTGCCTCGCTCCATGCTCGCGCCCCGAAACCCTGGCCTCGACGGCGCAGTGGGAGTGCCCGGACTGTACCTCGATCTCCGGCGGGGCTCCGGCGGTGAAGTCCGGCCAGTCCGGGGACTTGATAGCCAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGACAGGAGCTGATGAGCGGAAAGGCGGAGGTGGAAGAGGAGGAGGAGGAGGAAAAGAAAGGAGCGAAGAAGGACGTGTTGGATGTTCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACTACACCATGCGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTCAAAAGATGGCAAGTCAACCCCGAATCAACTCTTCCCTTGTAATTGCCATACGTATGGCTAAGCTTTCAAAATCACATGTTACTGGGGGGCCTGCCAAGGTATATCATTACATACAAAACCAAGATAGGCCTGACAAAGCATACACTACTGAGCGGGCACAAAAGACTGGAAAAGCTAATGCTGCAAGTGGCAAGATATTTGTTACAATTCCTTCGGATCATTTTGGACCTATACCTGCTGAAAATGATCCTGAGAGGAATCAAGGTGTCATGGTTGGGGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACACTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGCGCACAGTCTGTTGTGCTTTCGGGAGGGTACCTTGATGATGAGGATCATGGAGAATGGTTCTTATACACAGGAAGCGGCGGAAGAGATTTAAGTGGGAACAAGCGAACTAATAAGGATCAGTCCTTTGACCAGACGTTTGAAAAGTATAACAAGGCGCTCCAAGTTAGTTGCCAAAAAGGCTATCCTGTAAGGGTGGTCAGATCGCACAAGGAGAAGCGCTCCTCATATGCTCCTGAGAAAGGGTTGCGGTATGACGGAGTTTATAGAATTGAGAAGTGTTGGAGAAAGGTGGGAGTCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAATGAACCTGCACCATGGACAAGTGATGAACATGGAGATCGGCCTAGACCACTACCTGCAATACCTGAGCTGGCAAAAGCCACAAACATAACAGAGAGGAATGCAACTCCATCATGGGACTTTGAGGAGAAAGATAGCTGCTGGAAATGGATAAAGGCACCACCTATCAGCAAAAAACCTGTTCAAACATTAGATCCTGCTGATCGAAAGAGGTCAAGGAAAGCAATGAGGAAAGCACAGAACATGACTGTACGAGAGAGGCTCTTGAAAGAATTTAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCCGTTACCACATCATGTGGTCATAACTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGTGGGTAAGAGTGTGATGACAGAAAGAAACAGAGGTGGAAGATCCCTTCGAACTCAAAAGAATGTACTGACCTGCCCTTCCTGTCCCACTGATGTCACTGATTTTGTTCAAAATCCCCAGGTGAACACTGAGATAATGAAAGCAATCGAGTCACTGAAAGAAGAGGAAGCAAATGTGGATTCAGCTGATGAATCAAGTGAAGAAATGGACATTCCTGACAATGAAAATAGCACAGAAATGCTTGAGGAAGCAGCTGTGATAGATGGTGAGCAGACTTCTCCAACGAAATGCAACCCCAAAGGCAGTCCCAAACAACTGAAAGTTGATACTGGTAATGACTCGCCAAAGAGTGATATTGTGGAGGTAAATGAAATATCAGCTGTTCAGACTAATGGTGTCAATGGAGATACTGAGATGGAAATTGCCAAGGAAAGCAATGAGGTACAAGCTCCAGTTGTGAAAAGAGGGAAAAAAACTGCTGGTCGAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAACAAAGAAGACTAAGGAAGCTGTGAGTGAAAAGAACGGCTCACCATCAAGTAATGAAGTTTCTGCTGTACACACTAATGATGTCAATGGAGATTCTGATTTAGATATAGCTAAGGAGATCAATTCTGAGATGGAAATTGCCAAGGAAAGTAATGAGGTACCAGCTCCAGTTGCAAAATGTGGGAAGAAAATTGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGTTAAGGAAGCTGCGAGTGAAGATAATGGCTTACCATCAAGTAATGAAGTTTCTGCCATCCAGACTAATGGTGTCAATGGAGATTCTGATTTAGATATAGCCAAGGAGAGCAATGAGTTACAGGCTCCAGTGGCAAAAAGTGGGAAGAAAGCTGCTGCTGGTCTAGTTGAAGGTGTTGGATCAAGGACCAGAGGAAGGAAGAAGGCTAAGGTAGCAGCGAGTGAAGAAAATGGTTCACCATCTAGTCCTCTCCATGTGGATGCGGATTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

883

Amino Acids

96.14

Weight (kDa)

7.93

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 136 - 180 4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 136 - 176 5.3e-06 Ring finger domain
zf-C3HC4 PF00097 137 - 175 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 137 - 175 3.4e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 267 - 416 5.3e-50 SAD/SRA domain
zf-RING_UBOX PF13445 509 - 538 2.2e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 509 - 535 4.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 7 cut(s) 95, 104, 128, 784, 1261, 1309, 1691
Acc65I GGTACC 2 cut(s) 933, 2287
AccB1I GGYRCC 3 cut(s) 933, 1402, 2287
AccBSI CCGCTC 2 cut(s) 321, 694
AccI GTMKAC 1 cut(s) 847
AccII CGCG 1 cut(s) 139
AclWI GGATC 8 cut(s) 244, 765, 782, 957, 1029, 1433, 1647, 2560
AcoI YGGCCR 4 cut(s) 223, 457, 2101, 2335
AcsI RAATTY 1 cut(s) 1517
AcuI CTGAAG 1 cut(s) 494
AdeI CACNNNGTG 1 cut(s) 108
AfaI GTAC 7 cut(s) 187, 935, 1497, 1677, 2055, 2199, 2289
AfeI AGCGCT 1 cut(s) 1124
AfiI CCNNNNNNNGG 6 cut(s) 29, 232, 490, 1145, 2364, 2568
AflIII ACRYGT 2 cut(s) 378, 616
AgsI TTSAA 8 cut(s) 406, 609, 1046, 1430, 1513, 1601, 1721, 2543
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 7 cut(s) 150, 252, 1203, 1539, 1728, 2123, 2357
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 2 cut(s) 532, 862
Alw26I GTCTC 1 cut(s) 430
Alw44I GTGCAC 1 cut(s) 858
AlwI GGATC 8 cut(s) 244, 765, 782, 957, 1029, 1433, 1647, 2560
AlwNI CAGNNNCTG 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 1124
AoxI GGCC 8 cut(s) 153, 223, 457, 629, 668, 1286, 2101, 2335
ApaLI GTGCAC 1 cut(s) 858
ApoI RAATTY 1 cut(s) 1517
ArsI GACNNNNNNTTYG 2 cut(s) 1025, 1057
Asp700I GAANNNNTTC 1 cut(s) 479
Asp718I GGTACC 2 cut(s) 933, 2287
AspLEI GCGC 6 cut(s) 7, 141, 166, 908, 1063, 1125
AspS9I GGNCC 3 cut(s) 629, 767, 2559
AsuC2I CCSGG 2 cut(s) 179, 232
AsuHPI GGTGA 7 cut(s) 226, 1545, 1555, 1744, 1889, 2163, 2601
AsuII TTCGAA 1 cut(s) 1660
AvaII GGWCC 2 cut(s) 767, 2559
BaeGI GKGCMC 3 cut(s) 179, 700, 862
BalI TGGCCA 2 cut(s) 2103, 2337
BanI GGYRCC 3 cut(s) 933, 1402, 2287
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 2144
Bbv12I GWGCWC 2 cut(s) 532, 862
BceAI ACGGC 7 cut(s) 46, 49, 52, 91, 177, 510, 2182
BciT130I CCWGG 7 cut(s) 152, 254, 1205, 1541, 1730, 2125, 2359
BcnI CCSGG 2 cut(s) 179, 232
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 3 cut(s) 1290, 2537, 2616
BfoI RGCGCY 2 cut(s) 1064, 1126
BfuAI ACCTGC 7 cut(s) 95, 104, 128, 784, 1261, 1309, 1691
BglI GCCNNNNNGGC 2 cut(s) 161, 1083
BlpI GCTNAGC 1 cut(s) 600
Bme1390I CCNGG 9 cut(s) 152, 179, 232, 254, 1205, 1541, 1730, 2125, 2359
Bme18I GGWCC 2 cut(s) 767, 2559
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 3 cut(s) 629, 767, 2559
BmiI GGNNCC 6 cut(s) 207, 630, 935, 1404, 2289, 2499
BmrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1205, 1541, 1730, 2125, 2359
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 1 cut(s) 2623
BmuI ACTGGG 1 cut(s) 633
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 2144
BplI GAGNNNNNCTC 4 cut(s) 1109, 1141, 1491, 1523
BpmI CTGGAG 3 cut(s) 2046, 2280, 2484
Bpu10I CCTNAGC 3 cut(s) 1311, 2241, 2580
Bpu1102I GCTNAGC 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 1660
BpuEI CTTGAG 1 cut(s) 1874
BpuMI CCSGG 2 cut(s) 179, 232
BsaAI YACGTR 1 cut(s) 593
BsaBI GATNNNNATC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 430
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 232, 490, 1145, 2364, 2568
Bse118I RCCGGY 1 cut(s) 1221
Bse1I ACTGG 7 cut(s) 226, 628, 712, 1945, 2063, 2297, 2501
Bse3DI GCAATG 3 cut(s) 1476, 2053, 2491
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 7 cut(s) 152, 254, 1205, 1541, 1730, 2125, 2359
BseGI GGATG 4 cut(s) 391, 400, 2430, 2638
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 6 cut(s) 29, 232, 490, 1145, 2364, 2568
BseMI GCAATG 3 cut(s) 1476, 2053, 2491
BseMII CTCAG 7 cut(s) 681, 783, 1131, 1302, 1731, 2013, 2247
BseNI ACTGG 7 cut(s) 226, 628, 712, 1945, 2063, 2297, 2501
BseRI GAGGAG 9 cut(s) 89, 299, 302, 356, 359, 362, 365, 1117, 1385
BseSI GKGCMC 3 cut(s) 179, 700, 862
BsgI GTGCAG 1 cut(s) 1239
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 155, 225, 459, 631, 670, 1288, 2103, 2337
BshNI GGYRCC 3 cut(s) 933, 1402, 2287
BsiHKAI GWGCWC 2 cut(s) 532, 862
BsiSI CCGG 7 cut(s) 179, 198, 209, 222, 231, 431, 1222
BslFI GGGAC 4 cut(s) 248, 1376, 1680, 1902
BslI CCNNNNNNNGG 6 cut(s) 29, 232, 490, 1145, 2364, 2568
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 4 cut(s) 248, 1376, 1680, 1902
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 8 cut(s) 155, 225, 459, 631, 670, 1288, 2103, 2337
Bso31I GGTCTC 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 1660
Bsp1286I GDGCHC 5 cut(s) 179, 208, 532, 700, 862
Bsp1407I TGTACA 1 cut(s) 2197
Bsp1720I GCTNAGC 1 cut(s) 600
Bsp19I CCATGG 2 cut(s) 901, 1259
BspANI GGCC 8 cut(s) 155, 225, 459, 631, 670, 1288, 2103, 2337
BspCNI CTCAG 7 cut(s) 682, 784, 1132, 1303, 1732, 2014, 2248
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 6 cut(s) 207, 630, 935, 1404, 2289, 2499
BspMI ACCTGC 7 cut(s) 95, 104, 128, 784, 1261, 1309, 1691
BspPI GGATC 8 cut(s) 244, 765, 782, 957, 1029, 1433, 1647, 2560
BspT104I TTCGAA 1 cut(s) 1660
BspT107I GGYRCC 3 cut(s) 933, 1402, 2287
BspTNI GGTCTC 1 cut(s) 430
BsrBI CCGCTC 2 cut(s) 321, 694
BsrDI GCAATG 3 cut(s) 1476, 2053, 2491
BsrFI RCCGGY 1 cut(s) 1221
BsrGI TGTACA 1 cut(s) 2197
BsrI ACTGG 7 cut(s) 226, 628, 712, 1945, 2063, 2297, 2501
BssAI RCCGGY 1 cut(s) 1221
BssT1I CCWWGG 8 cut(s) 636, 901, 1259, 2037, 2104, 2271, 2338, 2475
Bst2UI CCWGG 7 cut(s) 152, 254, 1205, 1541, 1730, 2125, 2359
Bst4CI ACNGT 4 cut(s) 185, 894, 912, 1495
Bst6I CTCTTC 4 cut(s) 333, 577, 982, 1767
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstAUI TGTACA 1 cut(s) 2197
BstBAI YACGTR 1 cut(s) 593
BstBI TTCGAA 1 cut(s) 1660
BstC8I GCNNGC 8 cut(s) 47, 114, 137, 633, 696, 1317, 2101, 2335
BstDSI CCRYGG 3 cut(s) 107, 901, 1259
BstENI CCTNNNNNAGG 1 cut(s) 1143
BstF5I GGATG 4 cut(s) 391, 400, 2430, 2638
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 2 cut(s) 1064, 1126
BstHHI GCGC 6 cut(s) 7, 141, 166, 908, 1063, 1125
BstMAI GTCTC 1 cut(s) 430
BstNI CCWGG 7 cut(s) 152, 254, 1205, 1541, 1730, 2125, 2359
BstNSI RCATGY 2 cut(s) 49, 620
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 9 cut(s) 150, 177, 230, 252, 1203, 1539, 1728, 2123, 2357
BstSLI GKGCMC 3 cut(s) 179, 700, 862
BstSNI TACGTA 1 cut(s) 593
BstUI CGCG 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 2144
BstX2I RGATCY 3 cut(s) 249, 1438, 1652
BstXI CCANNNNNNTGG 5 cut(s) 595, 878, 2111, 2345, 2441
BstYI RGATCY 3 cut(s) 249, 1438, 1652
BsuRI GGCC 8 cut(s) 155, 225, 459, 631, 670, 1288, 2103, 2337
BtgI CCRYGG 3 cut(s) 107, 901, 1259
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 4 cut(s) 391, 400, 2430, 2638
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 6 cut(s) 173, 1698, 1707, 1737, 1763, 2508
BveI ACCTGC 7 cut(s) 95, 104, 128, 784, 1261, 1309, 1691
Cac8I GCNNGC 8 cut(s) 47, 114, 137, 633, 696, 1317, 2101, 2335
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 6 cut(s) 7, 141, 166, 908, 1063, 1125
Cfr10I RCCGGY 1 cut(s) 1221
Cfr13I GGNCC 3 cut(s) 629, 767, 2559
CseI GACGC 1 cut(s) 88
Csp6I GTAC 7 cut(s) 186, 934, 1496, 1676, 2054, 2198, 2288
CviQI GTAC 7 cut(s) 186, 934, 1496, 1676, 2054, 2198, 2288
DraI TTTAAA 1 cut(s) 2644
DraIII CACNNNGTG 1 cut(s) 108
EaeI YGGCCR 4 cut(s) 223, 457, 2101, 2335
Eam1104I CTCTTC 4 cut(s) 333, 577, 982, 1767
EarI CTCTTC 4 cut(s) 333, 577, 982, 1767
EciI GGCGGA 3 cut(s) 281, 344, 999
Eco105I TACGTA 1 cut(s) 593
Eco130I CCWWGG 8 cut(s) 636, 901, 1259, 2037, 2104, 2271, 2338, 2475
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 767, 2559
Eco47III AGCGCT 1 cut(s) 1124
Eco57I CTGAAG 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 1143
EcoO109I RGGNCCY 1 cut(s) 629
EcoRII CCWGG 7 cut(s) 150, 252, 1203, 1539, 1728, 2123, 2357
EcoT14I CCWWGG 8 cut(s) 636, 901, 1259, 2037, 2104, 2271, 2338, 2475
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 8 cut(s) 636, 901, 1259, 2037, 2104, 2271, 2338, 2475
FaqI GGGAC 4 cut(s) 248, 1376, 1680, 1902
FauI CCCGC 2 cut(s) 194, 687
FauNDI CATATG 1 cut(s) 1132
FblI GTMKAC 1 cut(s) 847
FokI GGATG 4 cut(s) 398, 407, 2417, 2645
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 1290, 2537, 2616
GlaI GCGC 6 cut(s) 6, 140, 165, 907, 1062, 1124
GsuI CTGGAG 3 cut(s) 2046, 2280, 2484
HaeII RGCGCY 2 cut(s) 1064, 1126
HaeIII GGCC 8 cut(s) 155, 225, 459, 631, 670, 1288, 2103, 2337
HapII CCGG 7 cut(s) 179, 198, 209, 222, 231, 431, 1222
HgaI GACGC 1 cut(s) 88
HhaI GCGC 6 cut(s) 7, 141, 166, 908, 1063, 1125
Hin6I GCGC 6 cut(s) 5, 139, 164, 906, 1061, 1123
HinP1I GCGC 6 cut(s) 5, 139, 164, 906, 1061, 1123
HincII GTYRAC 2 cut(s) 557, 848
HindII GTYRAC 2 cut(s) 557, 848
HindIII AAGCTT 1 cut(s) 602
HpaII CCGG 7 cut(s) 179, 198, 209, 222, 231, 431, 1222
HphI GGTGA 7 cut(s) 226, 1545, 1555, 1744, 1889, 2163, 2601
Hpy166II GTNNAC 6 cut(s) 557, 848, 860, 1735, 2200, 2609
Hpy188I TCNGA 8 cut(s) 70, 757, 1106, 1238, 1998, 2227, 2257, 2461
Hpy188III TCNNGA 8 cut(s) 392, 484, 791, 927, 1139, 1510, 1826, 2434
Hpy8I GTNNAC 6 cut(s) 557, 848, 860, 1735, 2200, 2609
Hpy99I CGWCG 4 cut(s) 32, 38, 74, 163
HpyCH4III ACNGT 4 cut(s) 185, 894, 912, 1495
HpyCH4IV ACGT 4 cut(s) 9, 378, 592, 1040
HpySE526I ACGT 4 cut(s) 9, 378, 592, 1040
HspAI GCGC 6 cut(s) 5, 139, 164, 906, 1061, 1123
KpnI GGTACC 2 cut(s) 937, 2291
LweI GCATC 1 cut(s) 2623
MaeI CTAG 3 cut(s) 1290, 2537, 2616
MaeII ACGT 4 cut(s) 9, 378, 592, 1040
MaeIII GTNAC 9 cut(s) 100, 442, 619, 742, 1241, 1558, 1705, 1761, 2490
MbiI CCGCTC 2 cut(s) 321, 694
MflI RGATCY 3 cut(s) 249, 1438, 1652
MhlI GDGCHC 5 cut(s) 179, 208, 532, 700, 862
MlsI TGGCCA 2 cut(s) 2103, 2337
MluNI TGGCCA 2 cut(s) 2103, 2337
MlyI GAGTC 4 cut(s) 281, 1209, 1769, 1943
MmeI TCCRAC 7 cut(s) 93, 363, 1166, 1916, 2092, 2326, 2530
Mox20I TGGCCA 2 cut(s) 2103, 2337
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 479
MscI TGGCCA 2 cut(s) 2103, 2337
MseI TTAA 4 cut(s) 995, 1212, 2376, 2643
MslI CAYNNNNRTG 5 cut(s) 513, 593, 1847, 2205, 2631
Msp20I TGGCCA 2 cut(s) 2103, 2337
MspA1I CMGCKG 4 cut(s) 427, 1796, 1865, 1991
MspI CCGG 7 cut(s) 179, 198, 209, 222, 231, 431, 1222
MspR9I CCNGG 9 cut(s) 152, 179, 232, 254, 1205, 1541, 1730, 2125, 2359
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 7 cut(s) 152, 254, 1205, 1541, 1730, 2125, 2359
MvnI CGCG 1 cut(s) 139
NciI CCSGG 2 cut(s) 179, 232
NcoI CCATGG 2 cut(s) 901, 1259
NdeI CATATG 1 cut(s) 1132
NlaIV GGNNCC 6 cut(s) 207, 630, 935, 1404, 2289, 2499
NmuCI GTSAC 5 cut(s) 100, 442, 1241, 1705, 1761
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 620
NspV TTCGAA 1 cut(s) 1660
PaeI GCATGC 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 128
PceI AGGCCT 1 cut(s) 670
PciI ACATGT 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1351
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 6 cut(s) 564, 799, 1790, 1802, 2222, 2456
PleI GAGTC 4 cut(s) 280, 1208, 1768, 1943
PpsI GAGTC 4 cut(s) 280, 1208, 1768, 1943
Ppu21I YACGTR 1 cut(s) 593
PscI ACATGT 1 cut(s) 616
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 7 cut(s) 150, 252, 1203, 1539, 1728, 2123, 2357
PspGI CCWGG 7 cut(s) 150, 252, 1203, 1539, 1728, 2123, 2357
PspN4I GGNNCC 6 cut(s) 207, 630, 935, 1404, 2289, 2499
PspPI GGNCC 3 cut(s) 629, 767, 2559
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 249, 1438, 1652
PvuII CAGCTG 4 cut(s) 427, 1796, 1865, 1991
RsaI GTAC 7 cut(s) 187, 935, 1497, 1677, 2055, 2199, 2289
RsaNI GTAC 7 cut(s) 186, 934, 1496, 1676, 2054, 2198, 2288
RseI CAYNNNNRTG 5 cut(s) 513, 593, 1847, 2205, 2631
SalI GTCGAC 1 cut(s) 846
SaqAI TTAA 4 cut(s) 995, 1212, 2376, 2643
Sau96I GGNCC 3 cut(s) 629, 767, 2559
SchI GAGTC 4 cut(s) 281, 1209, 1769, 1943
ScrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1205, 1541, 1730, 2125, 2359
SduI GDGCHC 5 cut(s) 179, 208, 532, 700, 862
SfaNI GCATC 1 cut(s) 2623
SfuI TTCGAA 1 cut(s) 1660
SinI GGWCC 2 cut(s) 767, 2559
SmiMI CAYNNNNRTG 5 cut(s) 513, 593, 1847, 2205, 2631
SmlI CTYRAG 1 cut(s) 1853
SmoI CTYRAG 1 cut(s) 1853
SnaBI TACGTA 1 cut(s) 593
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 670
SspMI CTAG 3 cut(s) 1290, 2537, 2616
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 9 cut(s) 150, 177, 230, 252, 1203, 1539, 1728, 2123, 2357
StyI CCWWGG 8 cut(s) 636, 901, 1259, 2037, 2104, 2271, 2338, 2475
TaaI ACNGT 4 cut(s) 185, 894, 912, 1495
TaiI ACGT 4 cut(s) 12, 381, 595, 1043
TaqI TCGA 7 cut(s) 158, 191, 847, 1450, 1660, 1758, 2095
TatI WGTACW 2 cut(s) 1675, 2197
TauI GCSGC 2 cut(s) 459, 984
TfiI GAWTC 6 cut(s) 564, 799, 1790, 1802, 2222, 2456
Tru1I TTAA 4 cut(s) 995, 1212, 2376, 2643
Tru9I TTAA 4 cut(s) 995, 1212, 2376, 2643
TscAI CASTG 6 cut(s) 173, 1705, 1714, 1744, 1770, 2508
TseFI GTSAC 5 cut(s) 100, 442, 1241, 1705, 1761
Tsp45I GTSAC 5 cut(s) 100, 442, 1241, 1705, 1761
TspDTI ATGAA 8 cut(s) 1263, 1287, 1763, 1815, 1848, 1995, 2199, 2433
TspGWI ACGGA 2 cut(s) 296, 1176
TspRI CASTG 6 cut(s) 173, 1705, 1714, 1744, 1770, 2508
VneI GTGCAC 1 cut(s) 858
VpaK11BI GGWCC 2 cut(s) 767, 2559
XagI CCTNNNNNAGG 1 cut(s) 1143
XapI RAATTY 1 cut(s) 1517
XceI RCATGY 2 cut(s) 49, 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 847
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 3 cut(s) 1290, 2537, 2616
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.