Rw7G008350

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000644
Physical Location & Seq
Forward (+)
7615537 .. 7617623
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008350.1

Sequence Viewer

Length: 1017 bp
ATGGCGCACGTTAGCCAGCTGCCATGCGACGGAGACGGCGTCTGCATGCTCTGCAAAACGACGCCGTCGCACGAGGACCAGCTCACCTGCAGGACCTGCGTCACGCCGTGGCACGTCAGCTGCCTCTCGTCCCGTCCCGAATCGCTGGCTTCCACTCTCCAGTGGGAGTGCCCGGACTGTTCCTCGCTCTCCGGCGACACGCCGGCCGTGAAATCGGCCGTGTCCGGCGAGCTGATCGCCGCGATCCGGGCGATCGAGGCGGATGCGGCGCTGACGGAGCAGCAGAAGGCGAGTAAGCGGCAGGAGCTGATGAGCGGGAAGGCTCTGCCGGAGGAAGAAGAAGGCGCCGGCAGGAAGGGAGTAAACGATGGCGTTTTGGATGGGATTTTCAATTGCTCGTTCTGTATGCAACTACCGGAGAGACCTGTTACGACACCATGTGGCCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCCATGCCATTCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGTTGCCATACGTATGGCTAAGATGTCAAAATCATATGCTACTGAGGGGCCTGCTAAGGTATACCACTTTGTCCGAAACCAAGACAGACCTGACAAAGCTTTCACTACCGAGCGGGCGCAGAAAACTGGGAAATCTAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGACCTATACCTGCTGAATATGATCCTGAGAGAAACCAAGGTGTGCTGGTAGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTTTTGCTCCCTTGTTTACATGGATCTGCATGGCAGTGGTGGAAGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

338

Amino Acids

36.78

Weight (kDa)

6.26

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 131 - 174 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 131 - 171 5.7e-07 Ring finger domain
zf-C3HC4 PF00097 132 - 170 1.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 170 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 132 - 170 1.1e-06 zinc finger of C3HC4-type, RING
zf-RING_UBOX PF13445 132 - 167 7.9e-06 RING-type zinc-finger
SAD_SRA PF02182 262 - 322 1.2e-19 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 3 cut(s) 95, 104, 769
Acc65I GGTACC 1 cut(s) 918
AccB1I GGYRCC 2 cut(s) 344, 918
AccBSI CCGCTC 2 cut(s) 315, 679
AccI GTMKAC 2 cut(s) 627, 832
AccII CGCG 1 cut(s) 242
AciI CCGC 8 cut(s) 240, 260, 266, 298, 315, 511, 547, 679
AclWI GGATC 4 cut(s) 238, 767, 942, 995
AcoI YGGCCR 3 cut(s) 204, 216, 442
AcyI GRCGYC 3 cut(s) 39, 62, 345
AdeI CACNNNGTG 2 cut(s) 108, 440
AfaI GTAC 2 cut(s) 496, 920
AfiI CCNNNNNNNGG 3 cut(s) 29, 246, 475
AgsI TTSAA 3 cut(s) 391, 460, 827
AjiI CACGTC 1 cut(s) 115
AluBI AGCT 8 cut(s) 19, 82, 120, 232, 307, 665, 740, 872
AluI AGCT 8 cut(s) 19, 82, 120, 232, 307, 665, 740, 872
Alw21I GWGCWC 2 cut(s) 847, 895
Alw26I GTCTC 2 cut(s) 27, 415
Alw44I GTGCAC 2 cut(s) 843, 891
AlwI GGATC 4 cut(s) 238, 767, 942, 995
AlwNI CAGNNNCTG 2 cut(s) 96, 307
AoxI GGCC 4 cut(s) 204, 216, 442, 614
ApaLI GTGCAC 2 cut(s) 843, 891
ApeKI GCWGC 4 cut(s) 19, 120, 280, 707
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 918
AspLEI GCGC 4 cut(s) 7, 271, 347, 685
AspS9I GGNCC 4 cut(s) 76, 93, 614, 752
AsuC2I CCSGG 2 cut(s) 173, 248
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 3 cut(s) 76, 93, 752
BaeGI GKGCMC 3 cut(s) 173, 847, 895
BalI TGGCCA 1 cut(s) 444
BanI GGYRCC 2 cut(s) 344, 918
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 2 cut(s) 847, 895
BbvI GCAGC 4 cut(s) 6, 107, 292, 694
BccI CCATC 2 cut(s) 362, 374
BceAI ACGGC 5 cut(s) 49, 52, 91, 191, 203
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 2 cut(s) 173, 248
BcoDI GTCTC 2 cut(s) 27, 415
BfmI CTRYAG 1 cut(s) 88
BfoI RGCGCY 2 cut(s) 272, 348
BfuAI ACCTGC 3 cut(s) 95, 104, 769
BisI GCNGC 8 cut(s) 20, 121, 240, 267, 281, 299, 511, 708
BlsI GCNGC 8 cut(s) 21, 122, 241, 268, 282, 300, 512, 709
Bme1390I CCNGG 2 cut(s) 173, 248
Bme18I GGWCC 3 cut(s) 76, 93, 752
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 76, 93, 614, 752
BmiI GGNNCC 3 cut(s) 346, 615, 920
BmrFI CCNGG 2 cut(s) 173, 248
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 2 cut(s) 253, 558
BmuI ACTGGG 1 cut(s) 702
BoxI GACNNNNGTC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 621
BpuMI CCSGG 2 cut(s) 173, 248
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 3 cut(s) 39, 62, 345
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 3 cut(s) 107, 787, 886
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 246, 475
Bse118I RCCGGY 2 cut(s) 202, 347
Bse1I ACTGG 2 cut(s) 160, 697
Bse8I GATNNNNATC 1 cut(s) 933
BseDI CCNNGG 3 cut(s) 107, 787, 886
BseGI GGATG 2 cut(s) 268, 385
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 3 cut(s) 29, 246, 475
BseMII CTCAG 2 cut(s) 600, 768
BseNI ACTGG 2 cut(s) 160, 697
BseSI GKGCMC 3 cut(s) 173, 847, 895
BseX3I CGGCCG 2 cut(s) 204, 216
BseXI GCAGC 4 cut(s) 6, 107, 292, 694
BseYI CCCAGC 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 3 cut(s) 207, 219, 255
BshFI GGCC 4 cut(s) 206, 218, 444, 616
BshNI GGYRCC 2 cut(s) 344, 918
BsiEI CGRYCG 3 cut(s) 207, 219, 255
BsiHKAI GWGCWC 2 cut(s) 847, 895
BsiSI CCGG 8 cut(s) 173, 192, 203, 225, 247, 329, 348, 416
BslFI GGGAC 2 cut(s) 115, 120
BslI CCNNNNNNNGG 3 cut(s) 29, 246, 475
BsmAI GTCTC 2 cut(s) 27, 415
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 115, 120
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 4 cut(s) 206, 218, 444, 616
Bso31I GGTCTC 1 cut(s) 415
Bsp1286I GDGCHC 3 cut(s) 173, 847, 895
Bsp143I GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 8 cut(s) 240, 260, 266, 298, 315, 511, 547, 679
BspANI GGCC 4 cut(s) 206, 218, 444, 616
BspCNI CTCAG 2 cut(s) 601, 769
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 3 cut(s) 346, 615, 920
BspMAI CTGCAG 1 cut(s) 92
BspMI ACCTGC 3 cut(s) 95, 104, 769
BspPI GGATC 4 cut(s) 238, 767, 942, 995
BspT107I GGYRCC 2 cut(s) 344, 918
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 315, 679
BsrFI RCCGGY 2 cut(s) 202, 347
BsrI ACTGG 2 cut(s) 160, 697
BssAI RCCGGY 2 cut(s) 202, 347
BssECI CCNNGG 3 cut(s) 107, 787, 886
BssMI GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
BssNAI GTATAC 1 cut(s) 628
BssNI GRCGYC 3 cut(s) 39, 62, 345
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 787, 886
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 71
Bst4CI ACNGT 4 cut(s) 179, 837, 879, 897
BstACI GRCGYC 3 cut(s) 39, 62, 345
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 8 cut(s) 17, 47, 147, 204, 230, 349, 618, 681
BstDEI CTNAG 4 cut(s) 585, 609, 621, 777
BstDSI CCRYGG 2 cut(s) 107, 886
BstF5I GGATG 2 cut(s) 268, 385
BstFNI CGCG 1 cut(s) 242
BstH2I RGCGCY 2 cut(s) 272, 348
BstHHI GCGC 4 cut(s) 7, 271, 347, 685
BstKTI GATC 7 cut(s) 237, 246, 255, 745, 775, 937, 990
BstMAI GTCTC 2 cut(s) 27, 415
BstMBI GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
BstMCI CGRYCG 3 cut(s) 207, 219, 255
BstMWI GCNNNNNNNGC 9 cut(s) 51, 96, 248, 257, 266, 277, 304, 462, 869
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 171, 246
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 3 cut(s) 173, 847, 895
BstSNI TACGTA 1 cut(s) 578
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 4 cut(s) 6, 107, 292, 694
BstX2I RGATCY 1 cut(s) 987
BstXI CCANNNNNNTGG 2 cut(s) 580, 863
BstYI RGATCY 1 cut(s) 987
BstZ17I GTATAC 1 cut(s) 628
BstZI CGGCCG 2 cut(s) 204, 216
BsuRI GGCC 4 cut(s) 206, 218, 444, 616
BtgI CCRYGG 2 cut(s) 107, 886
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 268, 385
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 3 cut(s) 167, 842, 1005
BveI ACCTGC 3 cut(s) 95, 104, 769
Cac8I GCNNGC 8 cut(s) 17, 47, 147, 204, 230, 349, 618, 681
CaiI CAGNNNCTG 2 cut(s) 96, 307
CfoI GCGC 4 cut(s) 7, 271, 347, 685
Cfr10I RCCGGY 2 cut(s) 202, 347
Cfr13I GGNCC 4 cut(s) 76, 93, 614, 752
CseI GACGC 3 cut(s) 28, 70, 88
Csp6I GTAC 2 cut(s) 495, 919
CviAII CATG 9 cut(s) 24, 46, 438, 515, 857, 887, 938, 984, 994
CviQI GTAC 2 cut(s) 495, 919
DdeI CTNAG 4 cut(s) 585, 609, 621, 777
DinI GGCGCC 1 cut(s) 346
DpnI GATC 7 cut(s) 236, 245, 254, 744, 774, 936, 989
DpnII GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
DraIII CACNNNGTG 2 cut(s) 108, 440
EaeI YGGCCR 3 cut(s) 204, 216, 442
EagI CGGCCG 2 cut(s) 204, 216
EciI GGCGGA 1 cut(s) 275
EclXI CGGCCG 2 cut(s) 204, 216
Eco105I TACGTA 1 cut(s) 578
Eco130I CCWWGG 2 cut(s) 787, 886
Eco31I GGTCTC 1 cut(s) 415
Eco47I GGWCC 3 cut(s) 76, 93, 752
Eco52I CGGCCG 2 cut(s) 204, 216
EcoO109I RGGNCCY 2 cut(s) 93, 614
EcoT14I CCWWGG 2 cut(s) 787, 886
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 2 cut(s) 787, 886
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 9 cut(s) 27, 49, 441, 518, 860, 890, 941, 987, 997
FaqI GGGAC 2 cut(s) 115, 120
FatI CATG 9 cut(s) 23, 45, 437, 514, 856, 886, 937, 983, 993
FauI CCCGC 3 cut(s) 308, 554, 672
FauNDI CATATG 1 cut(s) 601
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 2 cut(s) 627, 832
Fnu4HI GCNGC 8 cut(s) 20, 121, 240, 267, 281, 299, 511, 708
FokI GGATG 2 cut(s) 275, 392
Fsp4HI GCNGC 8 cut(s) 20, 121, 240, 267, 281, 299, 511, 708
GlaI GCGC 4 cut(s) 6, 270, 346, 684
GluI GCNGC 8 cut(s) 20, 121, 240, 267, 281, 299, 511, 708
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 2 cut(s) 272, 348
HaeIII GGCC 4 cut(s) 206, 218, 444, 616
HapII CCGG 8 cut(s) 173, 192, 203, 225, 247, 329, 348, 416
HgaI GACGC 3 cut(s) 28, 70, 88
HhaI GCGC 4 cut(s) 7, 271, 347, 685
Hin1I GRCGYC 3 cut(s) 39, 62, 345
Hin1II CATG 9 cut(s) 27, 49, 441, 518, 860, 890, 941, 987, 997
Hin6I GCGC 4 cut(s) 5, 269, 345, 683
HinP1I GCGC 4 cut(s) 5, 269, 345, 683
HincII GTYRAC 2 cut(s) 542, 833
HindII GTYRAC 2 cut(s) 542, 833
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 1 cut(s) 140
HpaII CCGG 8 cut(s) 173, 192, 203, 225, 247, 329, 348, 416
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 7 cut(s) 364, 542, 628, 833, 845, 893, 981
Hpy188I TCNGA 1 cut(s) 641
Hpy188III TCNNGA 4 cut(s) 137, 469, 776, 912
Hpy8I GTNNAC 7 cut(s) 364, 542, 628, 833, 845, 893, 981
Hpy99I CGWCG 3 cut(s) 32, 64, 70
HpyAV CCTTC 6 cut(s) 280, 313, 335, 349, 534, 1001
HpyCH4III ACNGT 4 cut(s) 179, 837, 879, 897
HpyCH4IV ACGT 3 cut(s) 9, 114, 577
HpyCH4V TGCA 9 cut(s) 45, 54, 90, 409, 503, 710, 845, 893, 993
HpyF10VI GCNNNNNNNGC 9 cut(s) 51, 96, 248, 257, 266, 277, 304, 462, 869
HpyF3I CTNAG 4 cut(s) 585, 609, 621, 777
HpySE526I ACGT 3 cut(s) 9, 114, 577
Hsp92I GRCGYC 3 cut(s) 39, 62, 345
Hsp92II CATG 9 cut(s) 27, 49, 441, 518, 860, 890, 941, 987, 997
HspAI GCGC 4 cut(s) 5, 269, 345, 683
KasI GGCGCC 1 cut(s) 344
KpnI GGTACC 1 cut(s) 922
KroI GCCGGC 2 cut(s) 202, 347
KroNI GCCGGC 2 cut(s) 204, 349
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
LmnI GCTCC 3 cut(s) 277, 304, 976
Lsp1109I GCAGC 4 cut(s) 6, 107, 292, 694
LweI GCATC 2 cut(s) 253, 558
MaeII ACGT 3 cut(s) 9, 114, 577
MaeIII GTNAC 3 cut(s) 100, 427, 727
MalI GATC 7 cut(s) 236, 245, 254, 744, 774, 936, 989
MbiI CCGCTC 2 cut(s) 315, 679
MboI GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
MboII GAAGA 3 cut(s) 347, 350, 549
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 987
MhlI GDGCHC 3 cut(s) 173, 847, 895
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 4 cut(s) 391, 448, 553, 732
MluNI TGGCCA 1 cut(s) 444
Mly113I GGCGCC 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 9 cut(s) 67, 134, 193, 250, 325, 604, 808, 908, 925
Mox20I TGGCCA 1 cut(s) 444
MroNI GCCGGC 2 cut(s) 202, 347
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 444
MslI CAYNNNNRTG 2 cut(s) 578, 998
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 3 cut(s) 19, 120, 740
MspI CCGG 8 cut(s) 173, 192, 203, 225, 247, 329, 348, 416
MspR9I CCNGG 2 cut(s) 173, 248
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 812
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 9 cut(s) 51, 96, 248, 257, 266, 277, 304, 462, 869
NaeI GCCGGC 2 cut(s) 204, 349
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 2 cut(s) 173, 248
NcoI CCATGG 1 cut(s) 886
NdeI CATATG 1 cut(s) 601
NdeII GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
NgoMIV GCCGGC 2 cut(s) 202, 347
NlaIII CATG 9 cut(s) 27, 49, 441, 518, 860, 890, 941, 987, 997
NlaIV GGNNCC 3 cut(s) 346, 615, 920
NmuCI GTSAC 2 cut(s) 100, 727
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 812
PdiI GCCGGC 2 cut(s) 204, 349
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 140
PflFI GACNNNGTC 2 cut(s) 38, 64
PkrI GCNGC 8 cut(s) 21, 122, 241, 268, 282, 300, 512, 709
Ple19I CGATCG 1 cut(s) 255
PluTI GGCGCC 1 cut(s) 348
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 810
PspN4I GGNNCC 3 cut(s) 346, 615, 920
PspPI GGNCC 4 cut(s) 76, 93, 614, 752
PspPPI RGGWCCY 1 cut(s) 93
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 96, 307
PsuI RGATCY 1 cut(s) 987
PsyI GACNNNGTC 2 cut(s) 38, 64
PvuI CGATCG 1 cut(s) 255
PvuII CAGCTG 3 cut(s) 19, 120, 740
RsaI GTAC 2 cut(s) 496, 920
RsaNI GTAC 2 cut(s) 495, 919
RseI CAYNNNNRTG 2 cut(s) 578, 998
SalI GTCGAC 1 cut(s) 831
SatI GCNGC 8 cut(s) 20, 121, 240, 267, 281, 299, 511, 708
Sau3AI GATC 7 cut(s) 234, 243, 252, 742, 772, 934, 987
Sau96I GGNCC 4 cut(s) 76, 93, 614, 752
SbfI CCTGCAGG 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 173, 248
SdaI CCTGCAGG 1 cut(s) 92
SduI GDGCHC 3 cut(s) 173, 847, 895
SfaNI GCATC 2 cut(s) 253, 558
SfcI CTRYAG 1 cut(s) 88
SfoI GGCGCC 1 cut(s) 346
SinI GGWCC 3 cut(s) 76, 93, 752
SmiMI CAYNNNNRTG 2 cut(s) 578, 998
SnaBI TACGTA 1 cut(s) 578
SphI GCATGC 1 cut(s) 49
Sse8387I CCTGCAGG 1 cut(s) 92
Sse9I AATT 4 cut(s) 391, 448, 553, 732
SsiI CCGC 8 cut(s) 240, 260, 266, 298, 315, 511, 547, 679
SspDI GGCGCC 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 171, 246
StyI CCWWGG 2 cut(s) 787, 886
TaaI ACNGT 4 cut(s) 179, 837, 879, 897
TaiI ACGT 3 cut(s) 12, 117, 580
TaqI TCGA 2 cut(s) 255, 832
TasI AATT 4 cut(s) 391, 448, 553, 732
TauI GCSGC 4 cut(s) 242, 269, 301, 513
TfiI GAWTC 1 cut(s) 140
TscAI CASTG 3 cut(s) 167, 842, 1005
TseFI GTSAC 2 cut(s) 100, 727
TseI GCWGC 4 cut(s) 19, 120, 280, 707
Tsp45I GTSAC 2 cut(s) 100, 727
TspGWI ACGGA 2 cut(s) 45, 290
TspRI CASTG 3 cut(s) 167, 842, 1005
Tth111I GACNNNGTC 2 cut(s) 38, 64
VneI GTGCAC 2 cut(s) 843, 891
VpaK11BI GGWCC 3 cut(s) 76, 93, 752
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 627, 832
XmnI GAANNNNTTC 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.