Rh6CG042700

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000644
Physical Location & Seq
Reverse (-)
4192061 .. 4202768
10708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG042700.1

Sequence Viewer

Length: 1191 bp
ATGGTTTCCGGGATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCATTTACACCGGGAGTGGTGGCAAGGATTTGAGTGGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGACGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTAAATGGTTATCCTGTTCGAGTAGTGAGATCGCACAAGGAGACACGCTCTTCGTATGCGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGGGTTATAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGTGATGATCATGGGGACCGTCCAAGACCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACTATGATGCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACTGGTGGATAGGGGAGAACCGGATGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAAGTTCTCAAAGGGTTTAGGTGTGATAAATGTCAGAAGATTATGACTGAACCAGTTACTACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCGGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGGTTAATAAGAATCTCGAGAACGCCATAGAATTACTCCAGCAGAAAGAGGAAGTGGAAGACGCTGAGGAAGATGAAGATGATGAAGAAGAGGAAGAAGAGGAAGAAGCTGAAGAAACTTACAATGCCGGTGAAGAGAGTGAAGCCGAAGACAAAGGAAGCATCCCACCAATACCTCAGACTGAAACTACAAAGCAACAAACCTGTGAGCAAAAGAAACCAAGTCATAAGCGAAATAGGTCGAGCAATGGAGACAACGTGTTGGATGGCGGAGCACTGACCAAGAGAAGCAAGAGGAGTAAAGTGGGAGCAGCTAGTAGTCTGATTGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

396

Amino Acids

44.72

Weight (kDa)

6.44

Isoelectric Point (pI)

60.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 2 - 127 1.3e-37 SAD/SRA domain
zf-RING_UBOX PF13445 217 - 248 5.7e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 46
AciI CCGC 3 cut(s) 322, 774, 1125
AcuI CTGAAG 1 cut(s) 987
AcyI GRCGYC 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 3 cut(s) 311, 451, 848
AjiI CACGTC 1 cut(s) 765
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AluBI AGCT 5 cut(s) 181, 716, 836, 965, 1169
AluI AGCT 5 cut(s) 181, 716, 836, 965, 1169
Alw21I GWGCWC 2 cut(s) 793, 1132
Alw26I GTCTC 2 cut(s) 242, 1101
Ama87I CYCGRG 1 cut(s) 872
ApeKI GCWGC 1 cut(s) 1166
ArsI GACNNNNNNTTYG 2 cut(s) 241, 273
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 3 cut(s) 38, 268, 391
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 2 cut(s) 10, 106
AsuHPI GGTGA 2 cut(s) 98, 998
AvaI CYCGRG 1 cut(s) 872
AvaII GGWCC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 2 cut(s) 921, 1011
Bbv12I GWGCWC 2 cut(s) 793, 1132
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 1 cut(s) 1178
BccI CCATC 4 cut(s) 287, 323, 572, 1115
BceAI ACGGC 1 cut(s) 620
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 2 cut(s) 10, 106
BcoDI GTCTC 2 cut(s) 242, 1101
BfaI CTAG 1 cut(s) 1170
BfoI RGCGCY 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 1 cut(s) 1167
BlsI GCNGC 1 cut(s) 1168
Bme1390I CCNGG 2 cut(s) 10, 106
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 765
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 37, 416
BmrFI CCNGG 2 cut(s) 10, 106
BmsI GCATC 2 cut(s) 493, 1026
BpiI GAAGAC 2 cut(s) 921, 1011
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 878
Bpu10I CCTNAGC 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 10, 106
BsaBI GATNNNNATC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 392, 769
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 2 cut(s) 1098, 1128
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 983
Bse1I ACTGG 2 cut(s) 561, 680
Bse3DI GCAATG 2 cut(s) 782, 1108
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 392, 769
BseGI GGATG 4 cut(s) 528, 583, 1017, 1126
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 2 cut(s) 782, 1108
BseMII CTCAG 4 cut(s) 69, 208, 912, 1046
BseNI ACTGG 2 cut(s) 561, 680
BseRI GAGGAG 1 cut(s) 1165
BseXI GCAGC 1 cut(s) 1178
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 793, 1132
BsiHKCI CYCGRG 1 cut(s) 872
BsiSI CCGG 4 cut(s) 9, 105, 575, 984
BslFI GGGAC 3 cut(s) 294, 428, 509
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 2 cut(s) 242, 1101
BsmFI GGGAC 3 cut(s) 294, 428, 509
BsmI GAATGC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 872
Bsp1286I GDGCHC 2 cut(s) 793, 1132
Bsp143I GATC 4 cut(s) 79, 163, 236, 406
BspACI CCGC 3 cut(s) 322, 774, 1125
BspCNI CTCAG 4 cut(s) 68, 207, 913, 1045
BspHI TCATGA 2 cut(s) 804, 1187
BspLI GGNNCC 2 cut(s) 37, 416
BspMI ACCTGC 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 782, 1108
BsrFI RCCGGY 1 cut(s) 983
BsrI ACTGG 2 cut(s) 561, 680
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 2 cut(s) 392, 769
BssMI GATC 4 cut(s) 79, 163, 236, 406
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 392, 769
Bst4CI ACNGT 4 cut(s) 86, 158, 419, 455
Bst6I CTCTTC 4 cut(s) 262, 939, 948, 984
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 517, 789
BstDEI CTNAG 6 cut(s) 55, 194, 200, 545, 921, 1032
BstF5I GGATG 4 cut(s) 528, 583, 1017, 1126
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 268, 391
BstKTI GATC 4 cut(s) 82, 166, 239, 409
BstMAI GTCTC 2 cut(s) 242, 1101
BstMBI GATC 4 cut(s) 79, 163, 236, 406
BstMWI GCNNNNNNNGC 2 cut(s) 33, 512
BstSCI CCNGG 2 cut(s) 8, 104
BstV1I GCAGC 1 cut(s) 1178
BstV2I GAAGAC 2 cut(s) 921, 1011
BtrI CACGTC 1 cut(s) 765
BtsCI GGATG 4 cut(s) 528, 583, 1017, 1126
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 4 cut(s) 29, 51, 460, 1130
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 517, 789
CciI TCATGA 2 cut(s) 804, 1187
CfoI GCGC 3 cut(s) 38, 268, 391
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 926
Csp6I GTAC 1 cut(s) 141
CviAII CATG 5 cut(s) 410, 693, 805, 827, 1188
CviJI RGCY 8 cut(s) 181, 199, 535, 716, 836, 965, 1001, 1169
CviKI_1 RGCY 8 cut(s) 181, 199, 535, 716, 836, 965, 1001, 1169
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 6 cut(s) 55, 194, 200, 545, 921, 1032
DinI GGCGCC 1 cut(s) 37
DpnI GATC 4 cut(s) 81, 165, 238, 408
DpnII GATC 4 cut(s) 79, 163, 236, 406
DraIII CACNNNGTG 1 cut(s) 695
Eam1104I CTCTTC 4 cut(s) 262, 939, 948, 984
EarI CTCTTC 4 cut(s) 262, 939, 948, 984
EciI GGCGGA 3 cut(s) 337, 789, 1140
Eco130I CCWWGG 2 cut(s) 392, 769
Eco32I GATATC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 987
Eco88I CYCGRG 1 cut(s) 872
EcoRV GATATC 1 cut(s) 359
EcoT14I CCWWGG 2 cut(s) 392, 769
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 392, 769
FaeI CATG 5 cut(s) 413, 696, 808, 830, 1191
FaqI GGGAC 3 cut(s) 294, 428, 509
FatI CATG 5 cut(s) 409, 692, 804, 826, 1187
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 1167
FokI GGATG 4 cut(s) 535, 590, 1004, 1133
Fsp4HI GCNGC 1 cut(s) 1167
FspBI CTAG 1 cut(s) 1170
GlaI GCGC 3 cut(s) 37, 267, 390
GluI GCNGC 1 cut(s) 1167
GsuI CTGGAG 1 cut(s) 878
HaeII RGCGCY 1 cut(s) 39
HapII CCGG 4 cut(s) 9, 105, 575, 984
HgaI GACGC 1 cut(s) 926
HhaI GCGC 3 cut(s) 38, 268, 391
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 5 cut(s) 413, 696, 808, 830, 1191
Hin6I GCGC 3 cut(s) 36, 266, 389
HinP1I GCGC 3 cut(s) 36, 266, 389
HindIII AAGCTT 2 cut(s) 179, 714
HinfI GANTC 2 cut(s) 868, 1184
HpaII CCGG 4 cut(s) 9, 105, 575, 984
HphI GGTGA 2 cut(s) 98, 998
Hpy166II GTNNAC 3 cut(s) 174, 301, 824
Hpy188I TCNGA 4 cut(s) 168, 663, 1035, 1179
Hpy188III TCNNGA 5 cut(s) 57, 805, 872, 874, 1188
Hpy8I GTNNAC 3 cut(s) 174, 301, 824
HpyAV CCTTC 1 cut(s) 498
HpyCH4III ACNGT 4 cut(s) 86, 158, 419, 455
HpyCH4IV ACGT 4 cut(s) 170, 764, 801, 1113
HpyCH4V TGCA 6 cut(s) 27, 354, 443, 506, 711, 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 512
HpyF3I CTNAG 6 cut(s) 55, 194, 200, 545, 921, 1032
HpySE526I ACGT 4 cut(s) 170, 764, 801, 1113
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 5 cut(s) 413, 696, 808, 830, 1191
HspAI GCGC 3 cut(s) 36, 266, 389
KasI GGCGCC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 4 cut(s) 79, 163, 236, 406
LguI GCTCTTC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 1127, 1163
Lsp1109I GCAGC 1 cut(s) 1178
LweI GCATC 2 cut(s) 493, 1026
MaeI CTAG 1 cut(s) 1170
MaeII ACGT 4 cut(s) 170, 764, 801, 1113
MaeIII GTNAC 3 cut(s) 189, 374, 682
MalI GATC 4 cut(s) 81, 165, 238, 408
MboI GATC 4 cut(s) 79, 163, 236, 406
MhlI GDGCHC 2 cut(s) 793, 1132
MluCI AATT 2 cut(s) 588, 887
Mly113I GGCGCC 1 cut(s) 36
MmeI TCCRAC 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 3 cut(s) 183, 749, 861
MspI CCGG 4 cut(s) 9, 105, 575, 984
MspR9I CCNGG 2 cut(s) 10, 106
Mva1269I GAATGC 1 cut(s) 622
MwoI GCNNNNNNNGC 2 cut(s) 33, 512
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 2 cut(s) 10, 106
NdeII GATC 4 cut(s) 79, 163, 236, 406
NlaIII CATG 5 cut(s) 413, 696, 808, 830, 1191
NlaIV GGNNCC 2 cut(s) 37, 416
NmuCI GTSAC 2 cut(s) 189, 374
PaeR7I CTCGAG 1 cut(s) 872
PagI TCATGA 2 cut(s) 804, 1187
PciSI GCTCTTC 1 cut(s) 262
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 868, 1184
PflMI CCANNNNNTGG 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 1 cut(s) 1168
PluTI GGCGCC 1 cut(s) 39
PsiI TTATAA 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 37, 416
PspPI GGNCC 1 cut(s) 415
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 183, 749, 861
SatI GCNGC 1 cut(s) 1167
Sau3AI GATC 4 cut(s) 79, 163, 236, 406
Sau96I GGNCC 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 10, 106
SduI GDGCHC 2 cut(s) 793, 1132
SfaNI GCATC 2 cut(s) 493, 1026
SfoI GGCGCC 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 872
SinI GGWCC 1 cut(s) 415
SlaI CTCGAG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 2 cut(s) 588, 887
SsiI CCGC 3 cut(s) 322, 774, 1125
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 1 cut(s) 1170
StyD4I CCNGG 2 cut(s) 8, 104
StyI CCWWGG 2 cut(s) 392, 769
TaaI ACNGT 4 cut(s) 86, 158, 419, 455
TaiI ACGT 4 cut(s) 173, 767, 804, 1116
TaqI TCGA 4 cut(s) 226, 538, 873, 1097
TasI AATT 2 cut(s) 588, 887
TfiI GAWTC 2 cut(s) 868, 1184
Tru1I TTAA 3 cut(s) 183, 749, 861
Tru9I TTAA 3 cut(s) 183, 749, 861
TscAI CASTG 4 cut(s) 29, 51, 460, 1137
TseFI GTSAC 2 cut(s) 189, 374
TseI GCWGC 1 cut(s) 1166
Tsp45I GTSAC 2 cut(s) 189, 374
TspDTI ATGAA 6 cut(s) 85, 90, 821, 945, 954, 1176
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 4 cut(s) 29, 51, 460, 1137
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.