Rh6DG038300

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000644
Physical Location & Seq
Reverse (-)
4109154 .. 4122408
13255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG038300.1

Sequence Viewer

Length: 927 bp
ATGGTTTCCGGGATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCATTTACACCGGGAGTGGTGGCAAGGATTTGAGTGGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGACGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTAAATGGTTATCCTGTTCGAGTAGTGAGATCGCACAAGGAGACACGCTCTTCGTATGCGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGGGTTATAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGTGATGATCATGGGGACCGTCCAAGACCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACTATGATGCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACTGGTGGATAGGGGAGAACCGGATGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAAGTTCTCAAAGGGTTTAGGTGTGATAAATGTCAGAAGATTATGACTGAACCAGTTACTACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCGGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGGTACGTCAAGGGCCTTGGCAAAGCTCCAGTAGTAATCAACGACTCATGAACAGGGCGCGGCACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

308

Amino Acids

35.11

Weight (kDa)

9.48

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 2 - 127 7.8e-38 SAD/SRA domain
zf-RING_UBOX PF13445 217 - 249 7.1e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 46
AccII CGCG 1 cut(s) 916
AciI CCGC 3 cut(s) 322, 774, 916
AcyI GRCGYC 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 2 cut(s) 142, 861
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 3 cut(s) 311, 451, 848
AjiI CACGTC 1 cut(s) 765
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AluBI AGCT 4 cut(s) 181, 716, 836, 882
AluI AGCT 4 cut(s) 181, 716, 836, 882
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 1 cut(s) 869
ArsI GACNNNNNNTTYG 2 cut(s) 241, 273
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 4 cut(s) 38, 268, 391, 916
AspS9I GGNCC 2 cut(s) 415, 869
AsuC2I CCSGG 2 cut(s) 10, 106
AsuHPI GGTGA 1 cut(s) 98
AvaII GGWCC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 793
BccI CCATC 3 cut(s) 287, 323, 572
BceAI ACGGC 1 cut(s) 620
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 2 cut(s) 10, 106
BcoDI GTCTC 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 1 cut(s) 917
BlsI GCNGC 1 cut(s) 918
Bme1390I CCNGG 2 cut(s) 10, 106
Bme18I GGWCC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 765
BmgT120I GGNCC 2 cut(s) 415, 869
BmiI GGNNCC 2 cut(s) 37, 416
BmrFI CCNGG 2 cut(s) 10, 106
BmsI GCATC 1 cut(s) 493
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 868
BpuMI CCSGG 2 cut(s) 10, 106
BsaBI GATNNNNATC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 392, 769, 872
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 3 cut(s) 561, 680, 885
Bse3DI GCAATG 1 cut(s) 782
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 392, 769, 872
BseGI GGATG 2 cut(s) 528, 583
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 782
BseMII CTCAG 2 cut(s) 69, 208
BseNI ACTGG 3 cut(s) 561, 680, 885
Bsh1236I CGCG 1 cut(s) 916
BshFI GGCC 1 cut(s) 871
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 3 cut(s) 9, 105, 575
BslFI GGGAC 3 cut(s) 294, 428, 509
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 3 cut(s) 294, 428, 509
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 1 cut(s) 871
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 4 cut(s) 79, 163, 236, 406
BspACI CCGC 3 cut(s) 322, 774, 916
BspANI GGCC 1 cut(s) 871
BspCNI CTCAG 2 cut(s) 68, 207
BspFNI CGCG 1 cut(s) 916
BspHI TCATGA 2 cut(s) 804, 903
BspLI GGNNCC 2 cut(s) 37, 416
BspMI ACCTGC 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 782
BsrI ACTGG 3 cut(s) 561, 680, 885
BssECI CCNNGG 3 cut(s) 392, 769, 872
BssMI GATC 4 cut(s) 79, 163, 236, 406
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 3 cut(s) 392, 769, 872
Bst4CI ACNGT 4 cut(s) 86, 158, 419, 455
Bst6I CTCTTC 1 cut(s) 262
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 517, 789
BstDEI CTNAG 4 cut(s) 55, 194, 200, 545
BstF5I GGATG 2 cut(s) 528, 583
BstFNI CGCG 1 cut(s) 916
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 4 cut(s) 38, 268, 391, 916
BstKTI GATC 4 cut(s) 82, 166, 239, 409
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 4 cut(s) 79, 163, 236, 406
BstMWI GCNNNNNNNGC 2 cut(s) 33, 512
BstSCI CCNGG 2 cut(s) 8, 104
BstUI CGCG 1 cut(s) 916
BsuRI GGCC 1 cut(s) 871
BtrI CACGTC 1 cut(s) 765
BtsCI GGATG 2 cut(s) 528, 583
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 3 cut(s) 29, 51, 460
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 517, 789
CciI TCATGA 2 cut(s) 804, 903
CfoI GCGC 4 cut(s) 38, 268, 391, 916
Cfr13I GGNCC 2 cut(s) 415, 869
Csp6I GTAC 2 cut(s) 141, 860
CviAII CATG 5 cut(s) 410, 693, 805, 827, 904
CviJI RGCY 7 cut(s) 181, 199, 535, 716, 836, 871, 882
CviKI_1 RGCY 7 cut(s) 181, 199, 535, 716, 836, 871, 882
CviQI GTAC 2 cut(s) 141, 860
DdeI CTNAG 4 cut(s) 55, 194, 200, 545
DinI GGCGCC 1 cut(s) 37
DpnI GATC 4 cut(s) 81, 165, 238, 408
DpnII GATC 4 cut(s) 79, 163, 236, 406
DraIII CACNNNGTG 1 cut(s) 695
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
EciI GGCGGA 2 cut(s) 337, 789
Eco130I CCWWGG 3 cut(s) 392, 769, 872
Eco32I GATATC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 869
EcoRV GATATC 1 cut(s) 359
EcoT14I CCWWGG 3 cut(s) 392, 769, 872
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 3 cut(s) 392, 769, 872
FaeI CATG 5 cut(s) 413, 696, 808, 830, 907
FaqI GGGAC 3 cut(s) 294, 428, 509
FatI CATG 5 cut(s) 409, 692, 804, 826, 903
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 917
FokI GGATG 2 cut(s) 535, 590
Fsp4HI GCNGC 1 cut(s) 917
GlaI GCGC 4 cut(s) 37, 267, 390, 915
GluI GCNGC 1 cut(s) 917
GsuI CTGGAG 1 cut(s) 868
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 1 cut(s) 871
HapII CCGG 3 cut(s) 9, 105, 575
HhaI GCGC 4 cut(s) 38, 268, 391, 916
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 5 cut(s) 413, 696, 808, 830, 907
Hin6I GCGC 4 cut(s) 36, 266, 389, 914
HinP1I GCGC 4 cut(s) 36, 266, 389, 914
HindIII AAGCTT 2 cut(s) 179, 714
HinfI GANTC 1 cut(s) 900
HpaII CCGG 3 cut(s) 9, 105, 575
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 3 cut(s) 174, 301, 824
Hpy188I TCNGA 3 cut(s) 168, 663, 926
Hpy188III TCNNGA 3 cut(s) 57, 805, 904
Hpy8I GTNNAC 3 cut(s) 174, 301, 824
HpyAV CCTTC 1 cut(s) 498
HpyCH4III ACNGT 4 cut(s) 86, 158, 419, 455
HpyCH4IV ACGT 4 cut(s) 170, 764, 801, 862
HpyCH4V TGCA 6 cut(s) 27, 354, 443, 506, 711, 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 512
HpyF3I CTNAG 4 cut(s) 55, 194, 200, 545
HpySE526I ACGT 4 cut(s) 170, 764, 801, 862
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 5 cut(s) 413, 696, 808, 830, 907
HspAI GCGC 4 cut(s) 36, 266, 389, 914
KasI GGCGCC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 4 cut(s) 79, 163, 236, 406
LguI GCTCTTC 1 cut(s) 262
LmnI GCTCC 1 cut(s) 887
LweI GCATC 1 cut(s) 493
MaeII ACGT 4 cut(s) 170, 764, 801, 862
MaeIII GTNAC 3 cut(s) 189, 374, 682
MalI GATC 4 cut(s) 81, 165, 238, 408
MboI GATC 4 cut(s) 79, 163, 236, 406
MboII GAAGA 6 cut(s) 89, 249, 595, 676, 789, 836
MhlI GDGCHC 1 cut(s) 793
MluCI AATT 1 cut(s) 588
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 1 cut(s) 894
MnlI CCTC 5 cut(s) 53, 268, 438, 546, 753
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 2 cut(s) 183, 749
MspI CCGG 3 cut(s) 9, 105, 575
MspR9I CCNGG 2 cut(s) 10, 106
Mva1269I GAATGC 1 cut(s) 622
MvnI CGCG 1 cut(s) 916
MwoI GCNNNNNNNGC 2 cut(s) 33, 512
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 2 cut(s) 10, 106
NdeII GATC 4 cut(s) 79, 163, 236, 406
NlaIII CATG 5 cut(s) 413, 696, 808, 830, 907
NlaIV GGNNCC 2 cut(s) 37, 416
NmuCI GTSAC 2 cut(s) 189, 374
PagI TCATGA 2 cut(s) 804, 903
PciSI GCTCTTC 1 cut(s) 262
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 630
PflMI CCANNNNNTGG 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 1 cut(s) 918
PleI GAGTC 1 cut(s) 894
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 894
PsiI TTATAA 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 37, 416
PspPI GGNCC 2 cut(s) 415, 869
RsaI GTAC 2 cut(s) 142, 861
RsaNI GTAC 2 cut(s) 141, 860
SapI GCTCTTC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 183, 749
SatI GCNGC 1 cut(s) 917
Sau3AI GATC 4 cut(s) 79, 163, 236, 406
Sau96I GGNCC 2 cut(s) 415, 869
SchI GAGTC 1 cut(s) 894
ScrFI CCNGG 2 cut(s) 10, 106
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 1 cut(s) 493
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 1 cut(s) 415
Sse9I AATT 1 cut(s) 588
SsiI CCGC 3 cut(s) 322, 774, 916
SspDI GGCGCC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 8, 104
StyI CCWWGG 3 cut(s) 392, 769, 872
TaaI ACNGT 4 cut(s) 86, 158, 419, 455
TaiI ACGT 4 cut(s) 173, 767, 804, 865
TaqI TCGA 2 cut(s) 226, 538
TasI AATT 1 cut(s) 588
TauI GCSGC 1 cut(s) 919
Tru1I TTAA 2 cut(s) 183, 749
Tru9I TTAA 2 cut(s) 183, 749
TscAI CASTG 3 cut(s) 29, 51, 460
TseFI GTSAC 2 cut(s) 189, 374
Tsp45I GTSAC 2 cut(s) 189, 374
TspDTI ATGAA 4 cut(s) 85, 90, 821, 920
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 3 cut(s) 29, 51, 460
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.