RchiOBHm_Chr6g0248841

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000644
Physical Location & Seq
Reverse (-)
4721733 .. 4722319
587 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22306

Sequence Viewer

Length: 504 bp
ATGTCTGACCTCATCACCGGCCTTCCCTGCAACTCCGACGGCCTCTGCATGGTGTGCAACCAAAAGCCGTCACCGGAGGACTTGTTGTCTTGCACCACGTGCGCCTCACCCTGGCACCTGACTTGCCTCTCCGTCAGTCCCGAAACCTCAGCCGACGCTCTGGTGTGGCAGTGTCCTGACTGCACTTCTCCGACAGCTTCGGTCGGTGCCAGAGGAGCAGGAAGCTCCGGCGATTTGGTGGCGGCCATCCGAGCTCTGGAGACCGATGAGTCGTTGAGTGAGGCTGAGAAGGCGAAGAAACGGCAGGAGCTGATGTCCAAAAACGTTTTAGCAACGTCCGATGGCAAAAGCAACAACTGTGTGAAGAGGACCAACGGCAGTCGTAATGATGCCCTCGATATTCTCATTAAGAGCTTAACCTGTTCAATTTGCTTCGAACTTCCGGAGAGGCCTGTTACGGTTAGTCCACCAGACTTGGTTCGACTCTGCACTACAGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

167

Amino Acids

17.62

Weight (kDa)

5.0

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 16 - 61 1.8e-07 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
AccB1I GGYRCC 2 cut(s) 114, 206
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 1 cut(s) 242
AclI AACGTT 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 243
AcvI CACGTG 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 99
AfiI CCNNNNNNNGG 3 cut(s) 49, 111, 256
AgsI TTSAA 1 cut(s) 426
AhdI GACNNNNNGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 5 cut(s) 197, 225, 254, 310, 414
AluI AGCT 5 cut(s) 197, 225, 254, 310, 414
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 310
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 4 cut(s) 19, 40, 243, 449
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 7, 63, 99
AsuII TTCGAA 1 cut(s) 435
AvaII GGWCC 1 cut(s) 369
BanI GGYRCC 2 cut(s) 114, 206
BanII GRGCYC 1 cut(s) 256
BbrPI CACGTG 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 256
BbvCI CCTCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 254, 335
BceAI ACGGC 4 cut(s) 52, 55, 317, 391
BciT130I CCWGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 369
BmeRI GACNNNNNGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 2 cut(s) 116, 208
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 379
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 110
BsaWI WCCGGW 2 cut(s) 73, 442
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 111, 256
Bse118I RCCGGY 1 cut(s) 17
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 3 cut(s) 49, 111, 256
BseMII CTCAG 2 cut(s) 162, 276
BseRI GAGGAG 1 cut(s) 228
BsgI GTGCAG 2 cut(s) 166, 472
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 4 cut(s) 21, 42, 245, 451
BshNI GGYRCC 2 cut(s) 114, 206
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 4 cut(s) 18, 74, 228, 443
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 49, 111, 256
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 4 cut(s) 21, 42, 245, 451
Bso31I GGTCTC 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 256
Bsp13I TCCGGA 1 cut(s) 442
BspACI CCGC 1 cut(s) 242
BspANI GGCC 4 cut(s) 21, 42, 245, 451
BspCNI CTCAG 2 cut(s) 161, 277
BspEI TCCGGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 116, 208
BspT104I TTCGAA 1 cut(s) 435
BspT107I GGYRCC 2 cut(s) 114, 206
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 359, 460
Bst6I CTCTTC 1 cut(s) 359
BstAPI GCANNNNNTGC 2 cut(s) 54, 99
BstBAI YACGTR 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 148, 285
BstF5I GGATG 1 cut(s) 246
BstHHI GCGC 1 cut(s) 104
BstMAI GTCTC 1 cut(s) 254
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 6 cut(s) 27, 54, 99, 215, 251, 290
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 492
BsuRI GGCC 4 cut(s) 21, 42, 245, 451
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 176
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 369
CseI GACGC 1 cut(s) 164
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 1 cut(s) 49
DdeI CTNAG 2 cut(s) 148, 285
DraIII CACNNNGTG 1 cut(s) 99
DrdI GACNNNNNNGTC 1 cut(s) 268
DriI GACNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 359
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 359
Ecl136II GAGCTC 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 254
Eco72I CACGTG 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 110
EcoT38I GRGCYC 1 cut(s) 256
FaeI CATG 1 cut(s) 52
FaiI YATR 1 cut(s) 50
FaqI GGGAC 1 cut(s) 123
FatI CATG 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 243
GlaI GCGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 4 cut(s) 21, 42, 245, 451
HapII CCGG 4 cut(s) 18, 74, 228, 443
HgaI GACGC 1 cut(s) 164
HhaI GCGC 1 cut(s) 104
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 2 cut(s) 269, 483
HpaII CCGG 4 cut(s) 18, 74, 228, 443
HphI GGTGA 3 cut(s) 7, 63, 99
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 5 cut(s) 7, 37, 192, 251, 340
Hpy188III TCNNGA 4 cut(s) 140, 176, 257, 443
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 2 cut(s) 41, 158
HpyAV CCTTC 2 cut(s) 32, 283
HpyCH4III ACNGT 2 cut(s) 359, 460
HpyCH4IV ACGT 3 cut(s) 98, 324, 335
HpyCH4V TGCA 6 cut(s) 30, 48, 57, 93, 183, 489
HpyF10VI GCNNNNNNNGC 6 cut(s) 27, 54, 99, 215, 251, 290
HpyF3I CTNAG 2 cut(s) 148, 285
HpySE526I ACGT 3 cut(s) 98, 324, 335
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 102
Kpn2I TCCGGA 1 cut(s) 442
LmnI GCTCC 3 cut(s) 215, 230, 307
LweI GCATC 1 cut(s) 379
MaeII ACGT 3 cut(s) 98, 324, 335
MaeIII GTNAC 2 cut(s) 69, 454
MboII GAAGA 2 cut(s) 307, 376
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 1 cut(s) 426
MlyI GAGTC 2 cut(s) 278, 477
MmeI TCCRAC 2 cut(s) 60, 215
MroI TCCGGA 1 cut(s) 442
MseI TTAA 2 cut(s) 408, 416
MspI CCGG 4 cut(s) 18, 74, 228, 443
MspR9I CCNGG 1 cut(s) 112
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 6 cut(s) 27, 54, 99, 215, 251, 290
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 2 cut(s) 116, 208
NmuCI GTSAC 1 cut(s) 69
NspV TTCGAA 1 cut(s) 435
PceI AGGCCT 1 cut(s) 451
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 2 cut(s) 277, 477
PmaCI CACGTG 1 cut(s) 99
PmlI CACGTG 1 cut(s) 99
PpsI GAGTC 2 cut(s) 277, 477
Ppu21I YACGTR 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 256
Psp1406I AACGTT 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 110
PspCI CACGTG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 116, 208
PspPI GGNCC 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 310
SacI GAGCTC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 408, 416
SatI GCNGC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 2 cut(s) 278, 477
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 435
SinI GGWCC 1 cut(s) 369
Sse9I AATT 1 cut(s) 426
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 1 cut(s) 242
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 1 cut(s) 110
TaaI ACNGT 2 cut(s) 359, 460
TaiI ACGT 3 cut(s) 101, 327, 338
TaqI TCGA 3 cut(s) 396, 435, 481
TaqII GACCGA 2 cut(s) 190, 278
TasI AATT 1 cut(s) 426
TauI GCSGC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 408, 416
Tru9I TTAA 2 cut(s) 408, 416
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 1 cut(s) 69
Tsp45I GTSAC 1 cut(s) 69
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.