Rroxscaffold_7G00212970

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000644
Physical Location & Seq
Forward (+)
63507382 .. 63509419
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212970.1

Sequence Viewer

Length: 642 bp
ATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGTGATGATCATGGGGACCGTCCAAGGCCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACTATGATTCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACTGGTGGATAGGGGAGAACTAGATGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAGTTCTCAAAGAAGATTATGACTGAACCAGTTACCACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCAGAAGAACATTGCGAGCACAAAAGAACGTCAGGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGACGCCGACCGTGGACTGTGCTCCTTCGTTCTCACACCTCCGATCTGATTTGGGTCAACCCACCGCCATATGGATTCAATTACTCACAACTGACCAAAACCATGGTAAGGCCTCTAGCTTGGATCACCGCTGCTCCATCGCCGGATTCCCGGACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

213

Amino Acids

23.97

Weight (kDa)

9.28

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_UBOX PF13445 96 - 131 8.8e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 33, 546, 610
AclWI GGATC 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 485
AdeI CACNNNGTG 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 552, 589
AgsI TTSAA 4 cut(s) 22, 100, 473, 560
AjiI CACGTC 1 cut(s) 390
AluBI AGCT 3 cut(s) 341, 461, 600
AluI AGCT 3 cut(s) 341, 461, 600
Alw21I GWGCWC 2 cut(s) 418, 505
AlwI GGATC 1 cut(s) 612
AoxI GGCC 2 cut(s) 74, 591
ApeKI GCWGC 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 1 cut(s) 64
Bbv12I GWGCWC 2 cut(s) 418, 505
BbvI GCAGC 1 cut(s) 599
BccI CCATC 3 cut(s) 34, 221, 626
BceAI ACGGC 1 cut(s) 269
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 632
BfaI CTAG 2 cut(s) 224, 597
BisI GCNGC 1 cut(s) 613
BlsI GCNGC 1 cut(s) 614
Bme1390I CCNGG 1 cut(s) 632
Bme18I GGWCC 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 71, 394, 492, 583
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 552, 589
Bse1I ACTGG 2 cut(s) 210, 305
Bse3DI GCAATG 1 cut(s) 407
BseDI CCNNGG 4 cut(s) 71, 394, 492, 583
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 552, 589
BseMI GCAATG 1 cut(s) 407
BseNI ACTGG 2 cut(s) 210, 305
BseXI GCAGC 1 cut(s) 599
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 2 cut(s) 76, 593
BsiEI CGRYCG 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 418, 505
BsiSI CCGG 2 cut(s) 624, 632
BslFI GGGAC 2 cut(s) 77, 158
BslI CCNNNNNNNGG 2 cut(s) 552, 589
BsmFI GGGAC 2 cut(s) 77, 158
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 2 cut(s) 76, 593
Bsp1286I GDGCHC 2 cut(s) 418, 505
Bsp143I GATC 3 cut(s) 55, 524, 604
Bsp19I CCATGG 1 cut(s) 583
BspACI CCGC 3 cut(s) 33, 546, 610
BspANI GGCC 2 cut(s) 76, 593
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 1 cut(s) 612
BsrDI GCAATG 1 cut(s) 407
BsrI ACTGG 2 cut(s) 210, 305
BssECI CCNNGG 4 cut(s) 71, 394, 492, 583
BssMI GATC 3 cut(s) 55, 524, 604
BssNI GRCGYC 1 cut(s) 485
BssT1I CCWWGG 3 cut(s) 71, 394, 583
Bst4CI ACNGT 5 cut(s) 50, 68, 104, 493, 500
BstACI GRCGYC 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 166, 414
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 2 cut(s) 492, 583
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 3 cut(s) 58, 527, 607
BstMBI GATC 3 cut(s) 55, 524, 604
BstMCI CGRYCG 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 630
BstV1I GCAGC 1 cut(s) 599
BstXI CCANNNNNNTGG 1 cut(s) 584
BsuRI GGCC 2 cut(s) 76, 593
BtgI CCRYGG 2 cut(s) 492, 583
BtgZI GCGATG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 55, 109
Cac8I GCNNGC 2 cut(s) 166, 414
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 493
CviAII CATG 4 cut(s) 59, 318, 452, 584
CviJI RGCY 6 cut(s) 76, 184, 341, 461, 593, 600
CviKI_1 RGCY 6 cut(s) 76, 184, 341, 461, 593, 600
DdeI CTNAG 1 cut(s) 194
DpnI GATC 3 cut(s) 57, 526, 606
DpnII GATC 3 cut(s) 55, 524, 604
DraIII CACNNNGTG 1 cut(s) 320
EciI GGCGGA 1 cut(s) 48
Eco130I CCWWGG 3 cut(s) 71, 394, 583
Eco147I AGGCCT 2 cut(s) 76, 593
Eco47I GGWCC 1 cut(s) 64
EcoT14I CCWWGG 3 cut(s) 71, 394, 583
ErhI CCWWGG 3 cut(s) 71, 394, 583
FaeI CATG 4 cut(s) 62, 321, 455, 587
FaqI GGGAC 2 cut(s) 77, 158
FatI CATG 4 cut(s) 58, 317, 451, 583
FauNDI CATATG 1 cut(s) 551
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 613
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 613
FspBI CTAG 2 cut(s) 224, 597
GluI GCNGC 1 cut(s) 613
HaeIII GGCC 2 cut(s) 76, 593
HapII CCGG 2 cut(s) 624, 632
HgaI GACGC 1 cut(s) 493
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 4 cut(s) 62, 321, 455, 587
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 3 cut(s) 152, 556, 627
HpaII CCGG 2 cut(s) 624, 632
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 4 cut(s) 12, 449, 496, 539
Hpy188I TCNGA 3 cut(s) 157, 524, 529
Hpy188III TCNNGA 1 cut(s) 430
Hpy8I GTNNAC 4 cut(s) 12, 449, 496, 539
HpyAV CCTTC 2 cut(s) 147, 516
HpyCH4III ACNGT 5 cut(s) 50, 68, 104, 493, 500
HpyCH4IV ACGT 2 cut(s) 389, 426
HpyCH4V TGCA 3 cut(s) 92, 336, 349
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 2 cut(s) 389, 426
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 4 cut(s) 62, 321, 455, 587
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 3 cut(s) 55, 524, 604
LmnI GCTCC 2 cut(s) 508, 620
LpnPI CCDG 8 cut(s) 96, 154, 191, 242, 318, 348, 415, 637
Lsp1109I GCAGC 1 cut(s) 599
MaeI CTAG 2 cut(s) 224, 597
MaeII ACGT 2 cut(s) 389, 426
MaeIII GTNAC 1 cut(s) 307
MalI GATC 3 cut(s) 57, 526, 606
MboI GATC 3 cut(s) 55, 524, 604
MboII GAAGA 4 cut(s) 244, 301, 414, 461
MhlI GDGCHC 2 cut(s) 418, 505
MluCI AATT 2 cut(s) 237, 560
MnlI CCTC 5 cut(s) 87, 195, 378, 530, 604
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 2 cut(s) 624, 632
MspR9I CCNGG 1 cut(s) 632
Mva1269I GAATGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 583
NdeI CATATG 1 cut(s) 551
NdeII GATC 3 cut(s) 55, 524, 604
NlaIII CATG 4 cut(s) 62, 321, 455, 587
NlaIV GGNNCC 1 cut(s) 65
PceI AGGCCT 2 cut(s) 76, 593
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 3 cut(s) 152, 556, 627
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 64
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 1 cut(s) 613
Sau3AI GATC 3 cut(s) 55, 524, 604
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 2 cut(s) 418, 505
SetI ASST 7 cut(s) 343, 359, 392, 429, 463, 522, 602
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 2 cut(s) 237, 560
SseBI AGGCCT 2 cut(s) 76, 593
SsiI CCGC 3 cut(s) 33, 546, 610
SspMI CTAG 2 cut(s) 224, 597
StuI AGGCCT 2 cut(s) 76, 593
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 3 cut(s) 71, 394, 583
TaaI ACNGT 5 cut(s) 50, 68, 104, 493, 500
TaiI ACGT 2 cut(s) 392, 429
TaqI TCGA 1 cut(s) 187
TasI AATT 2 cut(s) 237, 560
TfiI GAWTC 3 cut(s) 152, 556, 627
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 2 cut(s) 55, 109
TseI GCWGC 1 cut(s) 612
TspRI CASTG 2 cut(s) 55, 109
VpaK11BI GGWCC 1 cut(s) 64
XspI CTAG 2 cut(s) 224, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.