Rh7BG270200

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000644
Physical Location & Seq
Reverse (-)
24768575 .. 24775036
6462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG270200.1

Sequence Viewer

Length: 2622 bp
ATGGCGCACGTTAGCCAACTGCCGTGCGACGGCGACGGCGTATGCATGCTCTGCAAGGCGAAGCCGTCCGACGAGGAGAAGCTGACCTGCAAGACCTGCGTCACGCCGTGGCACGCCGCCTGCCTCTCTCCCTGCTCGCGCCCCGAAACCCTGGCCTCGACGGCGCAGTGGGAGTGCCCGGACTGTACCTCGATCTCCGGCGGGGCTCCGGCGGTGAAGTCCGGCGAGTCCGGGGACTTGATAGCCAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGACAGGAGCTGATGAGCGGAAAGGCGGAGGCGGAGGAGGAGGAGGCAAAGAAAGGAGCGAAGAAGGACGTGTTGGATGTTCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACGACACCATGCGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTCAAAAGATGGCAAGTCAACCCCGAATCAACTCTTCCCTTGTAATTGCCATACGTACGGCTAAGCTTTCAAAATCACATGTTACTGGGGCGGCTGCCAAGGTATATCATTACATACAAAACCAAGACAGGCCTGACAAAGCATACACTACTGAGCGAGCACAAAAGACTGGAAAAGCTAATGCTGCAAGTGGCAAGATATTTGTTACAATTCCTTCAGATCATTTTGGACCTATACCTGCTGAAAATGATCCTGAGAGAAATCAAGGTGTCATGGTTGGGGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACATTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGCGCACAGTCTGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAATGGTTCTTATACACAGGAAGTGGCGGAAGAGATTTGAGTGGGAACAAGCGAACTAATAAGGACCAGTCCTTTGACCAGACTTTTGAAAAGTATAACAAGGCGCTCCAAGTTAGTTGCCAGAAAGGCTATCCTGTAAGGGTGGTAAGATCGCACAAGGAGAAGCGCTCCGCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAGTTTATAGAATTGAGAAGTGTTGGAGAAAGGTGGGAGTCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAACGAACCTGCACCATGGACAAGTGATGAACATGGAGATCGGCCTAGACCACTACCAGCAATACCTGAGCTGGCAAAAGCCACAAACATAACAGAGAGGAATGCAAGTCCATCATGGGACTTTGAGGAGAAAGATGGCCGCTGGAAATGGATAAAGGCACCACCTATCAGCAAAAAACCTGTTCAAACATTAGATCCTGCTGATCGAAAGAGGTCAAGGAAAGCAATGAGGAGAGCACAGAACATGACTGTACGGGAGAGGCTCTTGAAAGAATTTAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCCGTTACCACATCATGTGGTCATAATTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGCGGGTAAGAGTGTGGTGACAGAAAGAAACAGAGGTGGAAGATCCCTTCGAACTCAAAGGAATGTACTGACCTGCCCTTCCTGTCCCACCGATGTCACTGATTTTGTTCAAAATCCCCAGGTGAACACAGAGATAATGAAAGCAATTGAGTCACTGAAAGAAGAGGAAGCGAATGTAGAATCAGCTGATGAATCAAGTGAAGAAATGGACATTCCTGACAATGAAAATAGCACAGAAATGCCAGAAGAAGCAGCTGTGGTAGATGGTGAGCAGACTTCTCCAACAAAATGCAACCCCAAAGGCAGTCCCAAACGACTGAAAGTTGATACTGGTAATGACTCGCCAAAGAGTGATATTGTGGAGGTAAATGAAATTTCTGCTGTCCAGACTAATGGTGTCAATGGAGATACTGAGATGGAAATTGCCAAGGAAAGCAATGAGGTACAGGCTCCAGTTGTGAAACGAGGGAAAAAAACTGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAACAAAGAAGGCTAAGGAAGCTGTGAGTGAAGAGAACGGCTCACCATCAAGTAATGAAGTTTCTTCTGTCCACACTAATGATGTCAATGGAGATTCAGATTTAGATATAGCTAAGGAGACCAATTCTGAGATGGAAGTTGCCAAGGAAAGTAATGAGGTACAGGCTCCAGTTGCGAAACGTGGGAAGAAAAATGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGTTAAGGAAGCTGTGAGTGAAGATAACGGCTCACCATCAAGTAATGAAGTTTCTGCTATCCAGACTAATGGTGTTAATGGAGATTCTGATTTAGATATAGCCAAGGAGAGCAATGAGTTGAAGAAAGCTGCTGCTGGTCTAGCTGTTGAAGGTGTTGGATCAAGGACCAGAGGAAGGAAGAAGGCTAAGGTAGCAGCGAGTGAAGAGAATGGTTCACCGTCTAGTCCTCTCCACGTGGATGCGGATTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

873

Amino Acids

95.1

Weight (kDa)

8.07

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 134 - 178 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 134 - 174 5.3e-06 Ring finger domain
zf-C3HC4 PF00097 135 - 173 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 135 - 173 3.4e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 265 - 414 5.4e-50 SAD/SRA domain
zf-RING_UBOX PF13445 507 - 531 1.6e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 507 - 533 3.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 95, 104, 778, 1255, 1685
Acc65I GGTACC 1 cut(s) 927
AccB1I GGYRCC 2 cut(s) 927, 1396
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 841
AccII CGCG 1 cut(s) 139
AclWI GGATC 6 cut(s) 244, 776, 951, 1427, 1641, 2533
AcoI YGGCCR 4 cut(s) 451, 1375, 2095, 2329
AcsI RAATTY 2 cut(s) 1511, 1977
AcuI CTGAAG 2 cut(s) 488, 732
AcvI CACGTG 1 cut(s) 2602
AdeI CACNNNGTG 1 cut(s) 108
AfaI GTAC 7 cut(s) 187, 589, 929, 1491, 1671, 2049, 2283
AfeI AGCGCT 1 cut(s) 1118
AfiI CCNNNNNNNGG 5 cut(s) 29, 484, 1139, 2358, 2541
AflIII ACRYGT 2 cut(s) 372, 610
AgsI TTSAA 9 cut(s) 400, 603, 1040, 1424, 1507, 1595, 1715, 2488, 2516
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 7 cut(s) 150, 252, 1197, 1533, 1722, 2117, 2351
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
Alw21I GWGCWC 4 cut(s) 526, 694, 856, 1477
Alw26I GTCTC 2 cut(s) 424, 2234
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 6 cut(s) 244, 776, 951, 1427, 1641, 2533
AlwNI CAGNNNCTG 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 1118
AoxI GGCC 7 cut(s) 153, 451, 662, 1280, 1375, 2095, 2329
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 9 cut(s) 418, 421, 626, 716, 1518, 1856, 2495, 2498, 2561
ApoI RAATTY 2 cut(s) 1511, 1977
ArsI GACNNNNNNTTYG 4 cut(s) 1007, 1019, 1039, 1051
Asp700I GAANNNNTTC 1 cut(s) 473
Asp718I GGTACC 1 cut(s) 927
AspLEI GCGC 6 cut(s) 7, 141, 166, 902, 1057, 1119
AspS9I GGNCC 3 cut(s) 761, 1015, 2532
AsuC2I CCSGG 2 cut(s) 179, 232
AsuHPI GGTGA 9 cut(s) 226, 1539, 1549, 1633, 1738, 1883, 2157, 2391, 2574
AsuII TTCGAA 1 cut(s) 1654
AvaII GGWCC 3 cut(s) 761, 1015, 2532
BaeGI GKGCMC 2 cut(s) 179, 856
BalI TGGCCA 2 cut(s) 2097, 2331
BanI GGYRCC 2 cut(s) 927, 1396
BanII GRGCYC 1 cut(s) 208
BbrPI CACGTG 1 cut(s) 2602
Bbv12I GWGCWC 4 cut(s) 526, 694, 856, 1477
BbvI GCAGC 9 cut(s) 408, 430, 613, 703, 1505, 1868, 2482, 2485, 2573
BciT130I CCWGG 7 cut(s) 152, 254, 1199, 1535, 1724, 2119, 2353
BcnI CCSGG 2 cut(s) 179, 232
BcoDI GTCTC 2 cut(s) 424, 2234
BfaI CTAG 3 cut(s) 1284, 2507, 2589
BfoI RGCGCY 2 cut(s) 1058, 1120
BfuAI ACCTGC 5 cut(s) 95, 104, 778, 1255, 1685
BglI GCCNNNNNGGC 2 cut(s) 161, 1077
BlpI GCTNAGC 1 cut(s) 594
Bme1390I CCNGG 9 cut(s) 152, 179, 232, 254, 1199, 1535, 1724, 2119, 2353
Bme18I GGWCC 3 cut(s) 761, 1015, 2532
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 3 cut(s) 761, 1015, 2532
BmiI GGNNCC 5 cut(s) 207, 929, 1398, 2055, 2289
BmrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1199, 1535, 1724, 2119, 2353
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 1 cut(s) 2596
BmuI ACTGGG 1 cut(s) 627
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 1103, 1135, 2147, 2179
BpmI CTGGAG 2 cut(s) 2040, 2274
Bpu10I CCTNAGC 4 cut(s) 1305, 2136, 2235, 2553
Bpu1102I GCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 1654
BpuMI CCSGG 2 cut(s) 179, 232
BsaAI YACGTR 2 cut(s) 587, 2602
BsaBI GATNNNNATC 1 cut(s) 942
BsaI GGTCTC 2 cut(s) 424, 2234
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 484, 1139, 2358, 2541
Bse118I RCCGGY 1 cut(s) 1215
Bse1I ACTGG 6 cut(s) 622, 706, 1018, 1939, 2057, 2291
Bse3DI GCAATG 3 cut(s) 1470, 2047, 2485
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 7 cut(s) 152, 254, 1199, 1535, 1724, 2119, 2353
BseGI GGATG 3 cut(s) 385, 394, 2611
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 5 cut(s) 29, 484, 1139, 2358, 2541
BseMI GCAATG 3 cut(s) 1470, 2047, 2485
BseMII CTCAG 6 cut(s) 675, 777, 1125, 1296, 2007, 2241
BseNI ACTGG 6 cut(s) 622, 706, 1018, 1939, 2057, 2291
BseRI GAGGAG 8 cut(s) 89, 299, 302, 353, 356, 359, 1379, 1483
BseSI GKGCMC 2 cut(s) 179, 856
BseXI GCAGC 9 cut(s) 408, 430, 613, 703, 1505, 1868, 2482, 2485, 2573
BsgI GTGCAG 1 cut(s) 1233
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 155, 453, 664, 1282, 1377, 2097, 2331
BshNI GGYRCC 2 cut(s) 927, 1396
BsiHKAI GWGCWC 4 cut(s) 526, 694, 856, 1477
BsiSI CCGG 7 cut(s) 179, 198, 209, 222, 231, 425, 1216
BsiWI CGTACG 1 cut(s) 587
BslFI GGGAC 4 cut(s) 248, 1370, 1674, 1896
BslI CCNNNNNNNGG 5 cut(s) 29, 484, 1139, 2358, 2541
BsmAI GTCTC 2 cut(s) 424, 2234
BsmFI GGGAC 4 cut(s) 248, 1370, 1674, 1896
BsmI GAATGC 1 cut(s) 1345
BsnI GGCC 7 cut(s) 155, 453, 664, 1282, 1377, 2097, 2331
Bso31I GGTCTC 2 cut(s) 424, 2234
Bsp119I TTCGAA 1 cut(s) 1654
Bsp1286I GDGCHC 6 cut(s) 179, 208, 526, 694, 856, 1477
Bsp1720I GCTNAGC 1 cut(s) 594
Bsp19I CCATGG 2 cut(s) 895, 1253
BspANI GGCC 7 cut(s) 155, 453, 664, 1282, 1377, 2097, 2331
BspCNI CTCAG 6 cut(s) 676, 778, 1126, 1297, 2008, 2242
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 5 cut(s) 207, 929, 1398, 2055, 2289
BspMI ACCTGC 5 cut(s) 95, 104, 778, 1255, 1685
BspPI GGATC 6 cut(s) 244, 776, 951, 1427, 1641, 2533
BspT104I TTCGAA 1 cut(s) 1654
BspT107I GGYRCC 2 cut(s) 927, 1396
BspTNI GGTCTC 2 cut(s) 424, 2234
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 3 cut(s) 1470, 2047, 2485
BsrFI RCCGGY 1 cut(s) 1215
BsrI ACTGG 6 cut(s) 622, 706, 1018, 1939, 2057, 2291
BssAI RCCGGY 1 cut(s) 1215
BssT1I CCWWGG 8 cut(s) 630, 895, 1253, 2031, 2098, 2265, 2332, 2469
Bst2UI CCWGG 7 cut(s) 152, 254, 1199, 1535, 1724, 2119, 2353
Bst4CI ACNGT 5 cut(s) 185, 888, 906, 1489, 2586
Bst6I CTCTTC 5 cut(s) 571, 976, 1761, 2148, 2565
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 2 cut(s) 587, 2602
BstBI TTCGAA 1 cut(s) 1654
BstC8I GCNNGC 8 cut(s) 47, 114, 121, 137, 690, 1311, 2095, 2329
BstDSI CCRYGG 3 cut(s) 107, 895, 1253
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 3 cut(s) 385, 394, 2611
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 2 cut(s) 1058, 1120
BstHHI GCGC 6 cut(s) 7, 141, 166, 902, 1057, 1119
BstMAI GTCTC 2 cut(s) 424, 2234
BstNI CCWGG 7 cut(s) 152, 254, 1199, 1535, 1724, 2119, 2353
BstNSI RCATGY 2 cut(s) 49, 614
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 9 cut(s) 150, 177, 230, 252, 1197, 1533, 1722, 2117, 2351
BstSLI GKGCMC 2 cut(s) 179, 856
BstSNI TACGTA 1 cut(s) 587
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 9 cut(s) 408, 430, 613, 703, 1505, 1868, 2482, 2485, 2573
BstX2I RGATCY 3 cut(s) 249, 1432, 1646
BstXI CCANNNNNNTGG 5 cut(s) 872, 1997, 2105, 2339, 2435
BstYI RGATCY 3 cut(s) 249, 1432, 1646
BsuRI GGCC 7 cut(s) 155, 453, 664, 1282, 1377, 2097, 2331
BtgI CCRYGG 3 cut(s) 107, 895, 1253
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 3 cut(s) 385, 394, 2611
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 3 cut(s) 173, 1701, 1757
BveI ACCTGC 5 cut(s) 95, 104, 778, 1255, 1685
Cac8I GCNNGC 8 cut(s) 47, 114, 121, 137, 690, 1311, 2095, 2329
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 6 cut(s) 7, 141, 166, 902, 1057, 1119
Cfr10I RCCGGY 1 cut(s) 1215
Cfr13I GGNCC 3 cut(s) 761, 1015, 2532
CseI GACGC 1 cut(s) 88
Csp6I GTAC 7 cut(s) 186, 588, 928, 1490, 1670, 2048, 2282
CviQI GTAC 7 cut(s) 186, 588, 928, 1490, 1670, 2048, 2282
DraI TTTAAA 1 cut(s) 2617
DraIII CACNNNGTG 1 cut(s) 108
EaeI YGGCCR 4 cut(s) 451, 1375, 2095, 2329
Eam1104I CTCTTC 5 cut(s) 571, 976, 1761, 2148, 2565
EarI CTCTTC 5 cut(s) 571, 976, 1761, 2148, 2565
EciI GGCGGA 4 cut(s) 281, 344, 350, 993
Eco105I TACGTA 1 cut(s) 587
Eco130I CCWWGG 8 cut(s) 630, 895, 1253, 2031, 2098, 2265, 2332, 2469
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 424, 2234
Eco47I GGWCC 3 cut(s) 761, 1015, 2532
Eco47III AGCGCT 1 cut(s) 1118
Eco57I CTGAAG 2 cut(s) 488, 732
Eco72I CACGTG 1 cut(s) 2602
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoRII CCWGG 7 cut(s) 150, 252, 1197, 1533, 1722, 2117, 2351
EcoT14I CCWWGG 8 cut(s) 630, 895, 1253, 2031, 2098, 2265, 2332, 2469
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 8 cut(s) 630, 895, 1253, 2031, 2098, 2265, 2332, 2469
FaqI GGGAC 4 cut(s) 248, 1370, 1674, 1896
FauI CCCGC 2 cut(s) 194, 1600
FauNDI CATATG 1 cut(s) 1126
FblI GTMKAC 1 cut(s) 841
FokI GGATG 3 cut(s) 392, 401, 2618
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 1284, 2507, 2589
GlaI GCGC 6 cut(s) 6, 140, 165, 901, 1056, 1118
GsuI CTGGAG 2 cut(s) 2040, 2274
HaeII RGCGCY 2 cut(s) 1058, 1120
HaeIII GGCC 7 cut(s) 155, 453, 664, 1282, 1377, 2097, 2331
HapII CCGG 7 cut(s) 179, 198, 209, 222, 231, 425, 1216
HgaI GACGC 1 cut(s) 88
HhaI GCGC 6 cut(s) 7, 141, 166, 902, 1057, 1119
Hin6I GCGC 6 cut(s) 5, 139, 164, 900, 1055, 1117
HinP1I GCGC 6 cut(s) 5, 139, 164, 900, 1055, 1117
HincII GTYRAC 2 cut(s) 551, 842
HindII GTYRAC 2 cut(s) 551, 842
HindIII AAGCTT 1 cut(s) 596
HpaII CCGG 7 cut(s) 179, 198, 209, 222, 231, 425, 1216
HphI GGTGA 9 cut(s) 226, 1539, 1549, 1633, 1738, 1883, 2157, 2391, 2574
Hpy166II GTNNAC 6 cut(s) 551, 842, 854, 1729, 2194, 2582
Hpy188I TCNGA 6 cut(s) 70, 751, 1232, 2221, 2251, 2455
Hpy188III TCNNGA 9 cut(s) 386, 439, 478, 785, 921, 1504, 1820, 1990, 2428
Hpy8I GTNNAC 6 cut(s) 551, 842, 854, 1729, 2194, 2582
Hpy99I CGWCG 4 cut(s) 32, 38, 74, 163
HpyCH4III ACNGT 5 cut(s) 185, 888, 906, 1489, 2586
HpyCH4IV ACGT 5 cut(s) 9, 372, 586, 2302, 2601
HpySE526I ACGT 5 cut(s) 9, 372, 586, 2302, 2601
HspAI GCGC 6 cut(s) 5, 139, 164, 900, 1055, 1117
KpnI GGTACC 1 cut(s) 931
Lsp1109I GCAGC 9 cut(s) 408, 430, 613, 703, 1505, 1868, 2482, 2485, 2573
LweI GCATC 1 cut(s) 2596
MaeI CTAG 3 cut(s) 1284, 2507, 2589
MaeII ACGT 5 cut(s) 9, 372, 586, 2302, 2601
MaeIII GTNAC 9 cut(s) 100, 436, 613, 736, 1235, 1552, 1621, 1699, 1755
MbiI CCGCTC 1 cut(s) 321
MfeI CAATTG 1 cut(s) 1749
MflI RGATCY 3 cut(s) 249, 1432, 1646
MhlI GDGCHC 6 cut(s) 179, 208, 526, 694, 856, 1477
MlsI TGGCCA 2 cut(s) 2097, 2331
MluNI TGGCCA 2 cut(s) 2097, 2331
MlyI GAGTC 5 cut(s) 236, 281, 1203, 1763, 1937
MmeI TCCRAC 7 cut(s) 93, 357, 1160, 1910, 2086, 2320, 2503
Mox20I TGGCCA 2 cut(s) 2097, 2331
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 473
MscI TGGCCA 2 cut(s) 2097, 2331
MseI TTAA 4 cut(s) 1206, 2370, 2442, 2616
MslI CAYNNNNRTG 4 cut(s) 507, 1841, 2199, 2604
Msp20I TGGCCA 2 cut(s) 2097, 2331
MspA1I CMGCKG 4 cut(s) 421, 1380, 1790, 1859
MspI CCGG 7 cut(s) 179, 198, 209, 222, 231, 425, 1216
MspR9I CCNGG 9 cut(s) 152, 179, 232, 254, 1199, 1535, 1724, 2119, 2353
MunI CAATTG 1 cut(s) 1749
Mva1269I GAATGC 1 cut(s) 1345
MvaI CCWGG 7 cut(s) 152, 254, 1199, 1535, 1724, 2119, 2353
MvnI CGCG 1 cut(s) 139
NciI CCSGG 2 cut(s) 179, 232
NcoI CCATGG 2 cut(s) 895, 1253
NdeI CATATG 1 cut(s) 1126
NlaIV GGNNCC 5 cut(s) 207, 929, 1398, 2055, 2289
NmuCI GTSAC 6 cut(s) 100, 436, 1235, 1621, 1699, 1755
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 614
NspV TTCGAA 1 cut(s) 1654
PaeI GCATGC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 664
PciI ACATGT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1345
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 5 cut(s) 558, 1784, 1796, 2216, 2450
Pfl23II CGTACG 1 cut(s) 587
PleI GAGTC 5 cut(s) 235, 280, 1202, 1762, 1937
PmaCI CACGTG 1 cut(s) 2602
PmlI CACGTG 1 cut(s) 2602
PpsI GAGTC 5 cut(s) 235, 280, 1202, 1762, 1937
Ppu21I YACGTR 2 cut(s) 587, 2602
PscI ACATGT 1 cut(s) 610
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 7 cut(s) 150, 252, 1197, 1533, 1722, 2117, 2351
PspCI CACGTG 1 cut(s) 2602
PspGI CCWGG 7 cut(s) 150, 252, 1197, 1533, 1722, 2117, 2351
PspLI CGTACG 1 cut(s) 587
PspN4I GGNNCC 5 cut(s) 207, 929, 1398, 2055, 2289
PspPI GGNCC 3 cut(s) 761, 1015, 2532
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 249, 1432, 1646
PvuII CAGCTG 3 cut(s) 421, 1790, 1859
RsaI GTAC 7 cut(s) 187, 589, 929, 1491, 1671, 2049, 2283
RsaNI GTAC 7 cut(s) 186, 588, 928, 1490, 1670, 2048, 2282
RseI CAYNNNNRTG 4 cut(s) 507, 1841, 2199, 2604
SalI GTCGAC 1 cut(s) 840
SaqAI TTAA 4 cut(s) 1206, 2370, 2442, 2616
Sau96I GGNCC 3 cut(s) 761, 1015, 2532
SchI GAGTC 5 cut(s) 236, 281, 1203, 1763, 1937
ScrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1199, 1535, 1724, 2119, 2353
SduI GDGCHC 6 cut(s) 179, 208, 526, 694, 856, 1477
SfaNI GCATC 1 cut(s) 2596
SfuI TTCGAA 1 cut(s) 1654
SinI GGWCC 3 cut(s) 761, 1015, 2532
SmiMI CAYNNNNRTG 4 cut(s) 507, 1841, 2199, 2604
SnaBI TACGTA 1 cut(s) 587
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 664
SspMI CTAG 3 cut(s) 1284, 2507, 2589
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 9 cut(s) 150, 177, 230, 252, 1197, 1533, 1722, 2117, 2351
StyI CCWWGG 8 cut(s) 630, 895, 1253, 2031, 2098, 2265, 2332, 2469
TaaI ACNGT 5 cut(s) 185, 888, 906, 1489, 2586
TaiI ACGT 5 cut(s) 12, 375, 589, 2305, 2604
TaqI TCGA 5 cut(s) 158, 191, 841, 1444, 1654
TatI WGTACW 1 cut(s) 1669
TauI GCSGC 4 cut(s) 119, 453, 626, 1380
TfiI GAWTC 5 cut(s) 558, 1784, 1796, 2216, 2450
Tru1I TTAA 4 cut(s) 1206, 2370, 2442, 2616
Tru9I TTAA 4 cut(s) 1206, 2370, 2442, 2616
TscAI CASTG 3 cut(s) 173, 1708, 1764
TseFI GTSAC 6 cut(s) 100, 436, 1235, 1621, 1699, 1755
TseI GCWGC 9 cut(s) 418, 421, 626, 716, 1518, 1856, 2495, 2498, 2561
Tsp45I GTSAC 6 cut(s) 100, 436, 1235, 1621, 1699, 1755
TspDTI ATGAA 7 cut(s) 1281, 1757, 1809, 1842, 1989, 2193, 2427
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 3 cut(s) 173, 1708, 1764
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 3 cut(s) 761, 1015, 2532
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 2 cut(s) 1511, 1977
XceI RCATGY 2 cut(s) 49, 614
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 2251
XmiI GTMKAC 1 cut(s) 841
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 3 cut(s) 1284, 2507, 2589
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.