Rw7G023820

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000644
Physical Location & Seq
Reverse (-)
28634764 .. 28640082
5319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023820.1

Sequence Viewer

Length: 2613 bp
ATGGCGCACGTTAGCCAACTGCCGTGCGACGGCGACGGCGTATGCATGCTCTGCAAGGCGAAGCCGTCCGACGAGGAGAAGCTGACCTGCAAGACCTGCGTCACCCCCTGGCACGCCGCCTGCCTCGCTCCCGGCTCGCGTCCCGAAACCCTGGCCTCGACGGCGCAGTGGGAGTGCCCGGACTGCACCTCGATCTCCGGCGGGGCTCCGACGGTGAAGTCCGGCGAGTCCGGGGACTTGATAGCCAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGACAGGAGCTGATGAGCGGAAAGGCGGAGGAGGAGGAGGAGGAAAAGAAAGGAGCGAAGAAGGACGTGTTGGATGTTCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACGACACCATGCGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTCAAAAGATGGCAAGTCAACCCCGAATCAACTCTTCCCTTGTAATTGCCATACGTATGGCTAAGCTTTCAAAATCACATGTTACTGGGGGGCCTGCCAAGGTATATCATTACATACAAAATCAAGACAGGCCTGACAAAGCATACACTACTGAGCGAGCACAAAAGACTGGAAAAGCAAATGCTGCAAGTGGCAAGATATTTGTTACAATTCCTTCAGATCATTTTGGACCTATACCTGCTGAAAATGATCCCGAGAGAAATCAAGGTGTCATGGTTGGGGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACATTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGCGCACAGTCTGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAATGGTTCTTATACACAGGAAGTGGCGGAAGAGATTTGAGTGGGAACAAGCGAACTAATAAGGACCAGTCCTTTGACCAGACTTTTGAAAAGTATAACAAGGCGCTCCAAGTTAGTTGCCAGAAAGGCTATCCTGTAAGGGTGGTAAGATCGCACAAGGAGAAGCGCTCTGCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAGTTTATAGAATTGAGAAGTGTTGGAGAAAGGTGGGAGTCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAATGAACCTGCACCATGGACAAGTGATGAACATGGAGATCAGCCTAGACCACTACCAGCAATACCTGAGCTGGCAAAAGCCACAAACATAACAGAGAGGAATGCAAGTCCGTCATGGGACTTTGAGGAGAAAGATGGCCGCTGGAAATGGATAAAGGCACCACCTATCAGCAAAAAACCTGTTCAAACATTAGATCCTGCTGATCGAAAGAGGTCAAGGAAAGCAATGAGGAGAGCACAGAACATGACTGTACGGGAGAGGCTCTTGAAAGAATTTAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCCATTACCACATCATGTGGTCATAATTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGCGGGTAAGAGTGTGGTGACAGAAAGAAACAGAGGTGGAAGATCCCTTCGAACTCAAAGGAATGTACTGACCTGCCCTTCCTGTCCCACCGATGTCACTGATTTTGTTCAAAATCCCCAGGTGAACACAGAGATAATGAAAGCAATTGAGTCACTGAAAGAAGAGGAAGCGAATGTAGAATCAGCTGATGAATCAAGTGAAGAAATGGACATTCCTGACAATGAAAATAGCACAGAAATGCCAGAAGAAGCAGCTGTGGTAGATGGTGAGCAGACTTCTCCAACAAAATGCAACCCCAAAGGCAGTCCCAAACGACTGAAAGTTGATACTGGTAATGACTCGCCAAAGAGTGATATTGTGGAGGTAAATGAAATTTCTGCTGTCCAGACTAATGGTGTCAATGGAGATACTGAGATGGAAATTGCCAAGGATAGCAATGAGGTACAGGCTCCAGTTGCGAAACGAGGGAAAAAAACTGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAACAAAGAAGGCTAAGGAAGCTGTGAGTGAAGAGAACGGCTCACCATCAAGTGATGAAGTTTCTGCTGTCCACACTAATGATGTCAATGGAGATTCTGATTTAGATATAGCTAAGGAGACCGATTCTGAGATGGAAGTTGCCAAGGAAAGTAATGAGAAAAATGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGTTAAGGAAGCTGTGAGTGAAGATAACGGCTCACCATCAAGTAATGAAGTTTCTGCTATCCAGACTAATGGTGTTTATGGAGATTCTGATTTAGATATAGCCAAGGAGAGCAATGAGTTACAGGCTCCAGTGGCAAAACGTAGGAACAAAGCTGCTGCTGGTCTAGCTGTTGAAGGTGTTGGATCAAGGACCAGGGGAAGGAAGAAGGCTAAGGTAGCAGCGAGTGAAGAGAATGGTTCACCATCTAGTCCTCTCCATGTGGATGTGGATTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

870

Amino Acids

94.96

Weight (kDa)

7.17

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 133 - 177 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 133 - 173 5.2e-06 Ring finger domain
zf-C3HC4 PF00097 134 - 172 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 134 - 172 3.4e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 264 - 413 5.4e-50 SAD/SRA domain
zf-RING_UBOX PF13445 506 - 530 3.7e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 506 - 532 7.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
Acc36I ACCTGC 5 cut(s) 95, 104, 775, 1252, 1682
Acc65I GGTACC 1 cut(s) 924
AccB1I GGYRCC 2 cut(s) 924, 1393
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 838
AccII CGCG 1 cut(s) 139
AclWI GGATC 6 cut(s) 244, 773, 948, 1424, 1638, 2524
AcoI YGGCCR 4 cut(s) 448, 1372, 2092, 2296
AcsI RAATTY 2 cut(s) 1508, 1974
AcuI CTGAAG 2 cut(s) 485, 729
AfaI GTAC 4 cut(s) 926, 1488, 1668, 2046
AfeI AGCGCT 1 cut(s) 1115
AfiI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2325, 2532
AflIII ACRYGT 2 cut(s) 369, 607
AgsI TTSAA 8 cut(s) 397, 600, 1037, 1421, 1504, 1592, 1712, 2507
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 9 cut(s) 107, 150, 252, 1194, 1530, 1719, 2114, 2318, 2525
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
Alw21I GWGCWC 4 cut(s) 523, 691, 853, 1474
Alw26I GTCTC 2 cut(s) 421, 2231
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 6 cut(s) 244, 773, 948, 1424, 1638, 2524
AlwNI CAGNNNCTG 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 782
Aor51HI AGCGCT 1 cut(s) 1115
AoxI GGCC 7 cut(s) 153, 448, 620, 659, 1372, 2092, 2296
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 8 cut(s) 415, 418, 713, 1515, 1853, 2486, 2489, 2552
ApoI RAATTY 2 cut(s) 1508, 1974
ArsI GACNNNNNNTTYG 4 cut(s) 1004, 1016, 1036, 1048
Asp700I GAANNNNTTC 1 cut(s) 470
Asp718I GGTACC 1 cut(s) 924
AspLEI GCGC 5 cut(s) 7, 166, 899, 1054, 1116
AspS9I GGNCC 4 cut(s) 620, 758, 1012, 2523
AsuC2I CCSGG 3 cut(s) 132, 179, 232
AsuII TTCGAA 1 cut(s) 1651
AvaI CYCGRG 1 cut(s) 782
AvaII GGWCC 3 cut(s) 758, 1012, 2523
BaeGI GKGCMC 2 cut(s) 179, 853
BalI TGGCCA 2 cut(s) 2094, 2298
BanI GGYRCC 2 cut(s) 924, 1393
BanII GRGCYC 1 cut(s) 208
Bbv12I GWGCWC 4 cut(s) 523, 691, 853, 1474
BbvI GCAGC 8 cut(s) 405, 427, 700, 1502, 1865, 2473, 2476, 2564
BceAI ACGGC 8 cut(s) 7, 46, 49, 52, 177, 501, 2173, 2377
BciT130I CCWGG 9 cut(s) 109, 152, 254, 1196, 1532, 1721, 2116, 2320, 2527
BcnI CCSGG 3 cut(s) 132, 179, 232
BcoDI GTCTC 2 cut(s) 421, 2231
BfaI CTAG 3 cut(s) 1281, 2498, 2580
BfoI RGCGCY 2 cut(s) 1055, 1117
BfuAI ACCTGC 5 cut(s) 95, 104, 775, 1252, 1682
BglI GCCNNNNNGGC 2 cut(s) 161, 1074
BlpI GCTNAGC 1 cut(s) 591
Bme18I GGWCC 3 cut(s) 758, 1012, 2523
BmeT110I CYCGRG 1 cut(s) 782
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 620, 758, 1012, 2523
BmiI GGNNCC 6 cut(s) 207, 621, 926, 1395, 2052, 2460
BmrI ACTGGG 1 cut(s) 624
BmuI ACTGGG 1 cut(s) 624
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 1100, 1132, 2144, 2176
BpmI CTGGAG 2 cut(s) 2037, 2445
Bpu10I CCTNAGC 4 cut(s) 1302, 2133, 2232, 2544
Bpu1102I GCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 1651
BpuMI CCSGG 3 cut(s) 132, 179, 232
BsaAI YACGTR 1 cut(s) 584
BsaBI GATNNNNATC 1 cut(s) 939
BsaI GGTCTC 2 cut(s) 421, 2231
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2325, 2532
Bse118I RCCGGY 1 cut(s) 1212
Bse1I ACTGG 6 cut(s) 619, 703, 1015, 1936, 2054, 2462
Bse3DI GCAATG 3 cut(s) 1467, 2044, 2452
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 9 cut(s) 109, 152, 254, 1196, 1532, 1721, 2116, 2320, 2527
BseGI GGATG 3 cut(s) 382, 391, 2602
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2325, 2532
BseMI GCAATG 3 cut(s) 1467, 2044, 2452
BseMII CTCAG 5 cut(s) 672, 1122, 1293, 2004, 2238
BseNI ACTGG 6 cut(s) 619, 703, 1015, 1936, 2054, 2462
BseRI GAGGAG 9 cut(s) 89, 299, 302, 347, 350, 353, 356, 1376, 1480
BseSI GKGCMC 2 cut(s) 179, 853
BseXI GCAGC 8 cut(s) 405, 427, 700, 1502, 1865, 2473, 2476, 2564
BsgI GTGCAG 2 cut(s) 169, 1230
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 155, 450, 622, 661, 1374, 2094, 2298
BshNI GGYRCC 2 cut(s) 924, 1393
BsiHKAI GWGCWC 4 cut(s) 523, 691, 853, 1474
BsiHKCI CYCGRG 1 cut(s) 782
BsiSI CCGG 7 cut(s) 132, 179, 198, 222, 231, 422, 1213
BslFI GGGAC 5 cut(s) 126, 248, 1367, 1671, 1893
BslI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2325, 2532
BsmAI GTCTC 2 cut(s) 421, 2231
BsmFI GGGAC 5 cut(s) 126, 248, 1367, 1671, 1893
BsmI GAATGC 1 cut(s) 1342
BsnI GGCC 7 cut(s) 155, 450, 622, 661, 1374, 2094, 2298
Bso31I GGTCTC 2 cut(s) 421, 2231
BsoBI CYCGRG 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 1651
Bsp1286I GDGCHC 6 cut(s) 179, 208, 523, 691, 853, 1474
Bsp1720I GCTNAGC 1 cut(s) 591
Bsp19I CCATGG 2 cut(s) 892, 1250
BspANI GGCC 7 cut(s) 155, 450, 622, 661, 1374, 2094, 2298
BspCNI CTCAG 5 cut(s) 673, 1123, 1294, 2005, 2239
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 6 cut(s) 207, 621, 926, 1395, 2052, 2460
BspMI ACCTGC 5 cut(s) 95, 104, 775, 1252, 1682
BspPI GGATC 6 cut(s) 244, 773, 948, 1424, 1638, 2524
BspT104I TTCGAA 1 cut(s) 1651
BspT107I GGYRCC 2 cut(s) 924, 1393
BspTNI GGTCTC 2 cut(s) 421, 2231
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 3 cut(s) 1467, 2044, 2452
BsrFI RCCGGY 1 cut(s) 1212
BsrI ACTGG 6 cut(s) 619, 703, 1015, 1936, 2054, 2462
BssAI RCCGGY 1 cut(s) 1212
BssT1I CCWWGG 8 cut(s) 627, 892, 1250, 2028, 2095, 2262, 2299, 2436
Bst2UI CCWGG 9 cut(s) 109, 152, 254, 1196, 1532, 1721, 2116, 2320, 2527
Bst4CI ACNGT 4 cut(s) 214, 885, 903, 1486
Bst6I CTCTTC 5 cut(s) 568, 973, 1758, 2145, 2556
BstAPI GCANNNNNTGC 3 cut(s) 51, 96, 713
BstBAI YACGTR 1 cut(s) 584
BstBI TTCGAA 1 cut(s) 1651
BstC8I GCNNGC 9 cut(s) 47, 114, 121, 137, 624, 687, 1308, 2092, 2296
BstDEI CTNAG 9 cut(s) 591, 681, 1131, 1302, 2013, 2133, 2232, 2247, 2544
BstDSI CCRYGG 2 cut(s) 892, 1250
BstENI CCTNNNNNAGG 1 cut(s) 1134
BstF5I GGATG 3 cut(s) 382, 391, 2602
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 2 cut(s) 1055, 1117
BstHHI GCGC 5 cut(s) 7, 166, 899, 1054, 1116
BstMAI GTCTC 2 cut(s) 421, 2231
BstNI CCWGG 9 cut(s) 109, 152, 254, 1196, 1532, 1721, 2116, 2320, 2527
BstNSI RCATGY 2 cut(s) 49, 611
BstPAI GACNNNNGTC 1 cut(s) 98
BstSLI GKGCMC 2 cut(s) 179, 853
BstSNI TACGTA 1 cut(s) 584
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 8 cut(s) 405, 427, 700, 1502, 1865, 2473, 2476, 2564
BstX2I RGATCY 3 cut(s) 249, 1429, 1643
BstXI CCANNNNNNTGG 6 cut(s) 586, 869, 1994, 2102, 2306, 2402
BstYI RGATCY 3 cut(s) 249, 1429, 1643
BsuRI GGCC 7 cut(s) 155, 450, 622, 661, 1374, 2094, 2298
BtgI CCRYGG 2 cut(s) 892, 1250
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 382, 391, 2602
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 4 cut(s) 173, 1698, 1754, 2469
BveI ACCTGC 5 cut(s) 95, 104, 775, 1252, 1682
Cac8I GCNNGC 9 cut(s) 47, 114, 121, 137, 624, 687, 1308, 2092, 2296
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 5 cut(s) 7, 166, 899, 1054, 1116
Cfr10I RCCGGY 1 cut(s) 1212
Cfr13I GGNCC 4 cut(s) 620, 758, 1012, 2523
CseI GACGC 2 cut(s) 88, 128
Csp6I GTAC 4 cut(s) 925, 1487, 1667, 2045
CviQI GTAC 4 cut(s) 925, 1487, 1667, 2045
DdeI CTNAG 9 cut(s) 591, 681, 1131, 1302, 2013, 2133, 2232, 2247, 2544
DraI TTTAAA 1 cut(s) 2608
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 4 cut(s) 448, 1372, 2092, 2296
Eam1104I CTCTTC 5 cut(s) 568, 973, 1758, 2145, 2556
EarI CTCTTC 5 cut(s) 568, 973, 1758, 2145, 2556
EciI GGCGGA 3 cut(s) 281, 344, 990
Eco105I TACGTA 1 cut(s) 584
Eco130I CCWWGG 8 cut(s) 627, 892, 1250, 2028, 2095, 2262, 2299, 2436
Eco147I AGGCCT 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 421, 2231
Eco47I GGWCC 3 cut(s) 758, 1012, 2523
Eco47III AGCGCT 1 cut(s) 1115
Eco57I CTGAAG 2 cut(s) 485, 729
Eco88I CYCGRG 1 cut(s) 782
EcoNI CCTNNNNNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 9 cut(s) 107, 150, 252, 1194, 1530, 1719, 2114, 2318, 2525
EcoT14I CCWWGG 8 cut(s) 627, 892, 1250, 2028, 2095, 2262, 2299, 2436
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 8 cut(s) 627, 892, 1250, 2028, 2095, 2262, 2299, 2436
FaqI GGGAC 5 cut(s) 126, 248, 1367, 1671, 1893
FauI CCCGC 2 cut(s) 194, 1597
FauNDI CATATG 1 cut(s) 1123
FblI GTMKAC 1 cut(s) 838
FokI GGATG 3 cut(s) 389, 398, 2609
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 1281, 2498, 2580
GlaI GCGC 5 cut(s) 6, 165, 898, 1053, 1115
GsuI CTGGAG 2 cut(s) 2037, 2445
HaeII RGCGCY 2 cut(s) 1055, 1117
HaeIII GGCC 7 cut(s) 155, 450, 622, 661, 1374, 2094, 2298
HapII CCGG 7 cut(s) 132, 179, 198, 222, 231, 422, 1213
HgaI GACGC 2 cut(s) 88, 128
HhaI GCGC 5 cut(s) 7, 166, 899, 1054, 1116
Hin6I GCGC 5 cut(s) 5, 164, 897, 1052, 1114
HinP1I GCGC 5 cut(s) 5, 164, 897, 1052, 1114
HincII GTYRAC 2 cut(s) 548, 839
HindII GTYRAC 2 cut(s) 548, 839
HindIII AAGCTT 1 cut(s) 593
HpaII CCGG 7 cut(s) 132, 179, 198, 222, 231, 422, 1213
Hpy166II GTNNAC 6 cut(s) 548, 839, 851, 1726, 2191, 2573
Hpy188I TCNGA 7 cut(s) 70, 210, 748, 1229, 2218, 2248, 2422
Hpy8I GTNNAC 6 cut(s) 548, 839, 851, 1726, 2191, 2573
Hpy99I CGWCG 5 cut(s) 32, 38, 74, 163, 214
HpyCH4III ACNGT 4 cut(s) 214, 885, 903, 1486
HpyCH4IV ACGT 4 cut(s) 9, 369, 583, 2473
HpyF3I CTNAG 9 cut(s) 591, 681, 1131, 1302, 2013, 2133, 2232, 2247, 2544
HpySE526I ACGT 4 cut(s) 9, 369, 583, 2473
HspAI GCGC 5 cut(s) 5, 164, 897, 1052, 1114
KpnI GGTACC 1 cut(s) 928
Lsp1109I GCAGC 8 cut(s) 405, 427, 700, 1502, 1865, 2473, 2476, 2564
MaeI CTAG 3 cut(s) 1281, 2498, 2580
MaeII ACGT 4 cut(s) 9, 369, 583, 2473
MaeIII GTNAC 9 cut(s) 100, 433, 610, 733, 1232, 1618, 1696, 1752, 2451
MbiI CCGCTC 1 cut(s) 321
MfeI CAATTG 1 cut(s) 1746
MflI RGATCY 3 cut(s) 249, 1429, 1643
MhlI GDGCHC 6 cut(s) 179, 208, 523, 691, 853, 1474
MlsI TGGCCA 2 cut(s) 2094, 2298
MluNI TGGCCA 2 cut(s) 2094, 2298
MlyI GAGTC 5 cut(s) 236, 281, 1200, 1760, 1934
MmeI TCCRAC 8 cut(s) 93, 233, 354, 1157, 1907, 2083, 2287, 2494
Mox20I TGGCCA 2 cut(s) 2094, 2298
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 470
MscI TGGCCA 2 cut(s) 2094, 2298
MseI TTAA 3 cut(s) 1203, 2337, 2607
MslI CAYNNNNRTG 5 cut(s) 504, 584, 1838, 2196, 2595
Msp20I TGGCCA 2 cut(s) 2094, 2298
MspA1I CMGCKG 4 cut(s) 418, 1377, 1787, 1856
MspI CCGG 7 cut(s) 132, 179, 198, 222, 231, 422, 1213
MunI CAATTG 1 cut(s) 1746
Mva1269I GAATGC 1 cut(s) 1342
MvaI CCWGG 9 cut(s) 109, 152, 254, 1196, 1532, 1721, 2116, 2320, 2527
MvnI CGCG 1 cut(s) 139
NciI CCSGG 3 cut(s) 132, 179, 232
NcoI CCATGG 2 cut(s) 892, 1250
NdeI CATATG 1 cut(s) 1123
NlaIV GGNNCC 6 cut(s) 207, 621, 926, 1395, 2052, 2460
NmuCI GTSAC 6 cut(s) 100, 433, 1232, 1618, 1696, 1752
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 611
NspV TTCGAA 1 cut(s) 1651
PaeI GCATGC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 661
PciI ACATGT 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1342
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 6 cut(s) 555, 1781, 1793, 2213, 2243, 2417
PleI GAGTC 5 cut(s) 235, 280, 1199, 1759, 1934
PpsI GAGTC 5 cut(s) 235, 280, 1199, 1759, 1934
Ppu21I YACGTR 1 cut(s) 584
PscI ACATGT 1 cut(s) 607
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 9 cut(s) 107, 150, 252, 1194, 1530, 1719, 2114, 2318, 2525
PspGI CCWGG 9 cut(s) 107, 150, 252, 1194, 1530, 1719, 2114, 2318, 2525
PspN4I GGNNCC 6 cut(s) 207, 621, 926, 1395, 2052, 2460
PspPI GGNCC 4 cut(s) 620, 758, 1012, 2523
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 249, 1429, 1643
PvuII CAGCTG 3 cut(s) 418, 1787, 1856
RsaI GTAC 4 cut(s) 926, 1488, 1668, 2046
RsaNI GTAC 4 cut(s) 925, 1487, 1667, 2045
RseI CAYNNNNRTG 5 cut(s) 504, 584, 1838, 2196, 2595
SalI GTCGAC 1 cut(s) 837
SaqAI TTAA 3 cut(s) 1203, 2337, 2607
Sau96I GGNCC 4 cut(s) 620, 758, 1012, 2523
SchI GAGTC 5 cut(s) 236, 281, 1200, 1760, 1934
SduI GDGCHC 6 cut(s) 179, 208, 523, 691, 853, 1474
SfuI TTCGAA 1 cut(s) 1651
SinI GGWCC 3 cut(s) 758, 1012, 2523
SmiMI CAYNNNNRTG 5 cut(s) 504, 584, 1838, 2196, 2595
SnaBI TACGTA 1 cut(s) 584
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 661
SspMI CTAG 3 cut(s) 1281, 2498, 2580
StuI AGGCCT 1 cut(s) 661
StyI CCWWGG 8 cut(s) 627, 892, 1250, 2028, 2095, 2262, 2299, 2436
TaaI ACNGT 4 cut(s) 214, 885, 903, 1486
TaiI ACGT 4 cut(s) 12, 372, 586, 2476
TaqI TCGA 5 cut(s) 158, 191, 838, 1441, 1651
TaqII GACCGA 1 cut(s) 2255
TatI WGTACW 1 cut(s) 1666
TauI GCSGC 3 cut(s) 119, 450, 1377
TfiI GAWTC 6 cut(s) 555, 1781, 1793, 2213, 2243, 2417
Tru1I TTAA 3 cut(s) 1203, 2337, 2607
Tru9I TTAA 3 cut(s) 1203, 2337, 2607
TscAI CASTG 4 cut(s) 173, 1705, 1761, 2469
TseFI GTSAC 6 cut(s) 100, 433, 1232, 1618, 1696, 1752
TseI GCWGC 8 cut(s) 415, 418, 713, 1515, 1853, 2486, 2489, 2552
Tsp45I GTSAC 6 cut(s) 100, 433, 1232, 1618, 1696, 1752
TspDTI ATGAA 8 cut(s) 1254, 1278, 1754, 1806, 1839, 1986, 2190, 2394
TspGWI ACGGA 2 cut(s) 296, 1335
TspRI CASTG 4 cut(s) 173, 1705, 1761, 2469
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 3 cut(s) 758, 1012, 2523
XagI CCTNNNNNAGG 1 cut(s) 1134
XapI RAATTY 2 cut(s) 1508, 1974
XceI RCATGY 2 cut(s) 49, 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 3 cut(s) 1281, 2498, 2580
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.