RLG00000015125

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000644
Physical Location & Seq
Forward (+)
62063658 .. 62086309
22652 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015125

Sequence Viewer

Length: 1959 bp
ATGTCTGACGTCATCACTGGCCTTCCCTGCAACTCCGACGGCCTCTGCATGGTGTGCAACCTAAAGCCGTCGCCGGAGAACTTGTTGTCTTGCACCACGTGCGCCTCACCCTGGCACCTGACTTGCCTCTCCATCCGTCCCGAAACCTCAGCCGACGCTCTGGTGTGGCAGTGTCCTGACTGCACTTCTCCGACGGCTTCGGTCGGTGCAGGAGGAGCCGGAAGCTCCGGCGATCTGGTGGCGGCCATCCGAGCTCTGGAGACCGATGAGTCGTTGAGTGAGGCTGAGAAGGCGAAGAAACGGCAGGAGCTGATGTCCAAAAACGTTTTAGCAACGTCCGATGGCGATGGCAACAACCGTGTGAAGAGGACCAACGGCAGTCGTAATGATGCCCTCGATATTCTCATTAAGAGCTTAACCTGTTCAATTTGCTTCGAACTTCCGGAGAGGCCTGTTACGACACCTTGCGGGCACAACTTCTGCCTCAAATGCTTTGAGAAATGGGTGGCGCAGGGTAAACTTACACGTGCAAATTGCCGCCAATCGATCCCCTCCACTCTGAGAATCAACTCTAATCTTGTGGGAAGCATTCGTATTGCCAAGGCATCACAACGCAGCGGCTCAGGGGAGTACCTACCTCAGTTGGCTCATCGCATTCGAAACCAGGACCGTCCGGACAAAGCATTTACAACCGAGCGAGCCAAGAAGCCTGGGAAAGCTAATGCCTCAAGTGGAAGGATCTTTGTGACATCAGCTCCGGATCATTTTGGGCCCATAACCGCCGAATATGATCCTGAGAAGAATAAGGGTGTGGCGGTGGGGGAGACTTGGGGGCATAGGATTGAATGTAGACAATGGGGTATTCATCGCCCTATGGTTTCCGGGATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCCTTTACACCGGGAATGGTGGCAAGGATTTGAGTGGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGACGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTAAATGGTTATCCTGTTCGAGTAGTGAGATCGCACAAGGACACACGCTCTTCGTATGTGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGGGTTATAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGGGACCGTCCAAGGCCTCTGCCATATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACAAAGGGTTCTCCTTCTTGGGACTATGATGCAGAAAAAGCGTGTTGGATGTGGAAAAAGCCTCAACCACTTAGCTGGTTTAGGTGTGACAAATGTCAGAAGATTATGACTGAACCACTTACTACTCCGTGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGAATAAGTTTAATAAAACAGAGGACGTGCCAAGGCAGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATAGCTGACTTTCTTCAAAATCCACAGGTTAATAAGAATCTCGAGAATGCCATAGAATTACTCCAGCAGAAAGAGGAAGATGTTGCAGAGGAAGTGGAAGACGCTGAGGAAGATGAAGAAGAGGAAGAAGCTAAAGAAACTTACAATGCCGGTGAAGAGAGTGAAGCCGAAGACAAAGGAAGCATCCCACCAATACCTCAGACTGAAACTACAGAGGAACAAACCTGTGAGCAAAAGAAACCAAGTCATAAGCGAAATAGGTCGACCAATGGAGACAATGTGTTGGATGGCGGAGCACTGACCAAGAGAAGCAAGAGGAGTAAAGTGGGACCAGCTAGTAGTCTGATTGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

653

Amino Acids

72.34

Weight (kDa)

8.11

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 16 - 61 2.6e-06 PHD-finger
zf-C3HC4_3 PF13920 139 - 183 2.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 140 - 172 9.3e-07 Ring finger domain
RING_XB3-XBAT31 PF24921 140 - 173 4.2e-06 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX PF13445 141 - 175 1.1e-07 RING-type zinc-finger
zf-C3HC4_2 PF13923 141 - 172 6.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 141 - 173 2.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 141 - 173 3.3e-07 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 268 - 418 2.8e-47 SAD/SRA domain
zf-RING_UBOX PF13445 474 - 509 2.2e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
AasI GACNNNNNNGTC 1 cut(s) 268
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 1267
AccB1I GGYRCC 2 cut(s) 114, 908
AccB7I CCANNNNNTGG 1 cut(s) 919
AccI GTMKAC 2 cut(s) 850, 1865
AccIII TCCGGA 3 cut(s) 442, 673, 757
AciI CCGC 8 cut(s) 242, 468, 538, 618, 780, 815, 1195, 1893
AclI AACGTT 1 cut(s) 324
AclWI GGATC 4 cut(s) 541, 746, 768, 785
AcoI YGGCCR 1 cut(s) 243
AcvI CACGTG 2 cut(s) 99, 527
AcyI GRCGYC 2 cut(s) 9, 909
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 2 cut(s) 632, 1015
AfiI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 5 cut(s) 426, 845, 1184, 1312, 1619
AjiI CACGTC 1 cut(s) 1536
AjnI CCWGG 3 cut(s) 110, 663, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
Alw21I GWGCWC 3 cut(s) 256, 1564, 1900
Alw26I GTCTC 3 cut(s) 254, 818, 1869
AlwI GGATC 4 cut(s) 541, 746, 768, 785
AlwNI CAGNNNCTG 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 1643
Aor13HI TCCGGA 3 cut(s) 442, 673, 757
AoxI GGCC 6 cut(s) 19, 40, 243, 449, 770, 1286
ApaI GGGCCC 1 cut(s) 774
ApeKI GCWGC 1 cut(s) 615
AspLEI GCGC 4 cut(s) 104, 511, 911, 1264
AspS9I GGNCC 6 cut(s) 369, 667, 770, 771, 1276, 1931
AsuC2I CCSGG 2 cut(s) 883, 979
AsuHPI GGTGA 3 cut(s) 99, 971, 1766
AsuII TTCGAA 2 cut(s) 435, 658
AvaI CYCGRG 1 cut(s) 1643
AvaII GGWCC 4 cut(s) 369, 667, 1276, 1931
BaeGI GKGCMC 2 cut(s) 474, 774
BaeI ACNNNNGTAYC 2 cut(s) 997, 1030
BanI GGYRCC 2 cut(s) 114, 908
BanII GRGCYC 2 cut(s) 256, 774
BbrPI CACGTG 2 cut(s) 99, 527
BbsI GAAGAC 2 cut(s) 1707, 1779
Bbv12I GWGCWC 3 cut(s) 256, 1564, 1900
BbvCI CCTCAGC 2 cut(s) 148, 1707
BbvI GCAGC 1 cut(s) 627
BccI CCATC 7 cut(s) 140, 254, 335, 341, 1160, 1196, 1883
BceAI ACGGC 5 cut(s) 52, 55, 210, 317, 391
BciT130I CCWGG 3 cut(s) 112, 665, 711
BcnI CCSGG 2 cut(s) 883, 979
BcoDI GTCTC 3 cut(s) 254, 818, 1869
BfaI CTAG 1 cut(s) 1938
BfmI CTRYAG 1 cut(s) 1812
BfoI RGCGCY 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 1267
BisI GCNGC 4 cut(s) 243, 538, 616, 619
BlsI GCNGC 4 cut(s) 244, 539, 617, 620
Bme1390I CCNGG 5 cut(s) 112, 665, 711, 883, 979
Bme18I GGWCC 4 cut(s) 369, 667, 1276, 1931
BmeT110I CYCGRG 1 cut(s) 1643
BmgBI CACGTC 1 cut(s) 1536
BmgT120I GGNCC 6 cut(s) 369, 667, 770, 771, 1276, 1931
BmiI GGNNCC 7 cut(s) 116, 217, 772, 910, 968, 1277, 1932
BmrFI CCNGG 5 cut(s) 112, 665, 711, 883, 979
BmsI GCATC 4 cut(s) 379, 614, 1354, 1794
BoxI GACNNNNGTC 1 cut(s) 1428
BpiI GAAGAC 2 cut(s) 1707, 1779
BpmI CTGGAG 2 cut(s) 278, 1649
Bpu10I CCTNAGC 3 cut(s) 148, 622, 1707
Bpu14I TTCGAA 2 cut(s) 435, 658
BpuEI CTTGAG 1 cut(s) 712
BpuMI CCSGG 2 cut(s) 883, 979
Bsa29I ATCGAT 1 cut(s) 545
BsaAI YACGTR 2 cut(s) 99, 527
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 9, 909
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1283, 1540
BsaWI WCCGGW 3 cut(s) 442, 673, 757
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 299, 329, 739, 769, 1866, 1896
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
Bse118I RCCGGY 1 cut(s) 1751
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 1553
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 3 cut(s) 442, 673, 757
BseBI CCWGG 3 cut(s) 112, 665, 711
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1283, 1540
BseGI GGATG 5 cut(s) 132, 246, 1389, 1785, 1894
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
BseMI GCAATG 1 cut(s) 1553
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 2 cut(s) 228, 1933
BseSI GKGCMC 2 cut(s) 474, 774
BseXI GCAGC 1 cut(s) 627
BsgI GTGCAG 2 cut(s) 166, 228
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1288
BshNI GGYRCC 2 cut(s) 114, 908
BshVI ATCGAT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 256, 1564, 1900
BsiHKCI CYCGRG 1 cut(s) 1643
BsiSI CCGG 9 cut(s) 74, 219, 228, 443, 674, 758, 882, 978, 1752
BslFI GGGAC 5 cut(s) 123, 1167, 1289, 1370, 1944
BslI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
BsmAI GTCTC 3 cut(s) 254, 818, 1869
BsmFI GGGAC 5 cut(s) 123, 1167, 1289, 1370, 1944
BsmI GAATGC 3 cut(s) 588, 654, 1654
BsnI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1288
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 1643
Bsp119I TTCGAA 2 cut(s) 435, 658
Bsp120I GGGCCC 1 cut(s) 770
Bsp1286I GDGCHC 5 cut(s) 256, 474, 774, 1564, 1900
Bsp13I TCCGGA 3 cut(s) 442, 673, 757
Bsp143I GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
BspACI CCGC 8 cut(s) 242, 468, 538, 618, 780, 815, 1195, 1893
BspANI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1288
BspDI ATCGAT 1 cut(s) 545
BspEI TCCGGA 3 cut(s) 442, 673, 757
BspHI TCATGA 2 cut(s) 1575, 1955
BspLI GGNNCC 7 cut(s) 116, 217, 772, 910, 968, 1277, 1932
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 4 cut(s) 541, 746, 768, 785
BspQI GCTCTTC 1 cut(s) 1135
BspT104I TTCGAA 2 cut(s) 435, 658
BspT107I GGYRCC 2 cut(s) 114, 908
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 1553
BsrFI RCCGGY 1 cut(s) 1751
BsrI ACTGG 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 1751
BssECI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1283, 1540
BssMI GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
BssNI GRCGYC 2 cut(s) 9, 909
BssT1I CCWWGG 4 cut(s) 600, 1265, 1283, 1540
Bst2UI CCWGG 3 cut(s) 112, 665, 711
Bst4CI ACNGT 6 cut(s) 359, 671, 959, 1031, 1280, 1316
Bst6I CTCTTC 4 cut(s) 359, 1135, 1716, 1752
BstACI GRCGYC 2 cut(s) 9, 909
BstAPI GCANNNNNTGC 2 cut(s) 54, 99
BstBAI YACGTR 2 cut(s) 99, 527
BstBI TTCGAA 2 cut(s) 435, 658
BstC8I GCNNGC 3 cut(s) 470, 699, 1560
BstF5I GGATG 5 cut(s) 132, 246, 1389, 1785, 1894
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 4 cut(s) 104, 511, 911, 1264
BstKTI GATC 8 cut(s) 235, 549, 741, 763, 793, 955, 1039, 1112
BstMAI GTCTC 3 cut(s) 254, 818, 1869
BstMBI GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1373
BstNI CCWGG 3 cut(s) 112, 665, 711
BstPAI GACNNNNGTC 1 cut(s) 1428
BstSCI CCNGG 5 cut(s) 110, 663, 709, 881, 977
BstSFI CTRYAG 1 cut(s) 1812
BstSLI GKGCMC 2 cut(s) 474, 774
BstV1I GCAGC 1 cut(s) 627
BstV2I GAAGAC 2 cut(s) 1707, 1779
BstX2I RGATCY 1 cut(s) 738
BstXI CCANNNNNNTGG 1 cut(s) 1410
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1288
BsuTUI ATCGAT 1 cut(s) 545
BtgZI GCGATG 3 cut(s) 360, 635, 851
BtrI CACGTC 1 cut(s) 1536
BtsCI GGATG 5 cut(s) 132, 246, 1389, 1785, 1894
BtsI GCAGTG 2 cut(s) 176, 902
BtsIMutI CAGTG 6 cut(s) 15, 176, 902, 924, 1321, 1898
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 3 cut(s) 470, 699, 1560
CaiI CAGNNNCTG 1 cut(s) 310
CciI TCATGA 2 cut(s) 1575, 1955
CfoI GCGC 4 cut(s) 104, 511, 911, 1264
Cfr10I RCCGGY 1 cut(s) 1751
Cfr13I GGNCC 6 cut(s) 369, 667, 770, 771, 1276, 1931
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 2 cut(s) 164, 1712
Csp6I GTAC 2 cut(s) 631, 1014
CviAII CATG 4 cut(s) 49, 1576, 1598, 1956
CviQI GTAC 2 cut(s) 631, 1014
DinI GGCGCC 1 cut(s) 910
DpnI GATC 8 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1111
DpnII GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
DraIII CACNNNGTG 1 cut(s) 99
DrdI GACNNNNNNGTC 1 cut(s) 268
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 4 cut(s) 359, 1135, 1716, 1752
EarI CTCTTC 4 cut(s) 359, 1135, 1716, 1752
EciI GGCGGA 2 cut(s) 1210, 1908
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 4 cut(s) 600, 1265, 1283, 1540
Eco147I AGGCCT 2 cut(s) 451, 1288
Eco24I GRGCYC 2 cut(s) 256, 774
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 1232
Eco47I GGWCC 4 cut(s) 369, 667, 1276, 1931
Eco53kI GAGCTC 1 cut(s) 254
Eco72I CACGTG 2 cut(s) 99, 527
Eco88I CYCGRG 1 cut(s) 1643
EcoICRI GAGCTC 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 110, 663, 709
EcoRV GATATC 1 cut(s) 1232
EcoT14I CCWWGG 4 cut(s) 600, 1265, 1283, 1540
EcoT38I GRGCYC 2 cut(s) 256, 774
EgeI GGCGCC 1 cut(s) 910
EheI GGCGCC 1 cut(s) 910
ErhI CCWWGG 4 cut(s) 600, 1265, 1283, 1540
FaeI CATG 4 cut(s) 52, 1579, 1601, 1959
FaqI GGGAC 5 cut(s) 123, 1167, 1289, 1370, 1944
FatI CATG 4 cut(s) 48, 1575, 1597, 1955
FauI CCCGC 1 cut(s) 461
FblI GTMKAC 2 cut(s) 850, 1865
Fnu4HI GCNGC 4 cut(s) 243, 538, 616, 619
FokI GGATG 5 cut(s) 119, 233, 1396, 1772, 1901
FriOI GRGCYC 2 cut(s) 256, 774
Fsp4HI GCNGC 4 cut(s) 243, 538, 616, 619
FspBI CTAG 1 cut(s) 1938
GlaI GCGC 4 cut(s) 103, 510, 910, 1263
GluI GCNGC 4 cut(s) 243, 538, 616, 619
GsuI CTGGAG 2 cut(s) 278, 1649
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 6 cut(s) 21, 42, 245, 451, 772, 1288
HapII CCGG 9 cut(s) 74, 219, 228, 443, 674, 758, 882, 978, 1752
HgaI GACGC 2 cut(s) 164, 1712
HhaI GCGC 4 cut(s) 104, 511, 911, 1264
Hin1I GRCGYC 2 cut(s) 9, 909
Hin1II CATG 4 cut(s) 52, 1579, 1601, 1959
Hin6I GCGC 4 cut(s) 102, 509, 909, 1262
HinP1I GCGC 4 cut(s) 102, 509, 909, 1262
HincII GTYRAC 1 cut(s) 1866
HindII GTYRAC 1 cut(s) 1866
HindIII AAGCTT 2 cut(s) 1052, 1485
HinfI GANTC 4 cut(s) 269, 564, 1639, 1952
HpaII CCGG 9 cut(s) 74, 219, 228, 443, 674, 758, 882, 978, 1752
HphI GGTGA 3 cut(s) 99, 971, 1766
Hpy166II GTNNAC 6 cut(s) 518, 851, 1047, 1174, 1595, 1866
Hpy8I GTNNAC 6 cut(s) 518, 851, 1047, 1174, 1595, 1866
Hpy99I CGWCG 4 cut(s) 41, 73, 158, 196
HpyAV CCTTC 4 cut(s) 32, 283, 729, 1359
HpyCH4III ACNGT 6 cut(s) 359, 671, 959, 1031, 1280, 1316
HpyCH4IV ACGT 8 cut(s) 9, 98, 324, 335, 526, 1043, 1535, 1572
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1373
HpySE526I ACGT 8 cut(s) 9, 98, 324, 335, 526, 1043, 1535, 1572
Hsp92I GRCGYC 2 cut(s) 9, 909
Hsp92II CATG 4 cut(s) 52, 1579, 1601, 1959
HspAI GCGC 4 cut(s) 102, 509, 909, 1262
KasI GGCGCC 1 cut(s) 908
Kpn2I TCCGGA 3 cut(s) 442, 673, 757
Kzo9I GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
LguI GCTCTTC 1 cut(s) 1135
LmnI GCTCC 5 cut(s) 215, 230, 307, 760, 1895
Lsp1109I GCAGC 1 cut(s) 627
LweI GCATC 4 cut(s) 379, 614, 1354, 1794
MaeI CTAG 1 cut(s) 1938
MaeII ACGT 8 cut(s) 9, 98, 324, 335, 526, 1043, 1535, 1572
MaeIII GTNAC 5 cut(s) 454, 745, 1062, 1247, 1421
MalI GATC 8 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1111
MboI GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 5 cut(s) 256, 474, 774, 1564, 1900
MluCI AATT 3 cut(s) 426, 532, 1658
Mly113I GGCGCC 1 cut(s) 909
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 4 cut(s) 60, 215, 1361, 1866
MroI TCCGGA 3 cut(s) 442, 673, 757
MseI TTAA 5 cut(s) 408, 416, 1056, 1520, 1632
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 9 cut(s) 74, 219, 228, 443, 674, 758, 882, 978, 1752
MspR9I CCNGG 5 cut(s) 112, 665, 711, 883, 979
Mva1269I GAATGC 3 cut(s) 588, 654, 1654
MvaI CCWGG 3 cut(s) 112, 665, 711
MwoI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1373
NarI GGCGCC 1 cut(s) 909
NciI CCSGG 2 cut(s) 883, 979
NdeII GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
NlaIII CATG 4 cut(s) 52, 1579, 1601, 1959
NlaIV GGNNCC 7 cut(s) 116, 217, 772, 910, 968, 1277, 1932
NmuCI GTSAC 4 cut(s) 745, 1062, 1247, 1421
NspV TTCGAA 2 cut(s) 435, 658
PaeR7I CTCGAG 1 cut(s) 1643
PagI TCATGA 2 cut(s) 1575, 1955
PceI AGGCCT 2 cut(s) 451, 1288
PciSI GCTCTTC 1 cut(s) 1135
PctI GAATGC 3 cut(s) 588, 654, 1654
PfeI GAWTC 3 cut(s) 564, 1639, 1952
PflMI CCANNNNNTGG 1 cut(s) 919
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 4 cut(s) 244, 539, 617, 620
PleI GAGTC 1 cut(s) 277
PluTI GGCGCC 1 cut(s) 912
PmaCI CACGTG 2 cut(s) 99, 527
PmlI CACGTG 2 cut(s) 99, 527
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 2 cut(s) 99, 527
PshAI GACNNNNGTC 1 cut(s) 1428
PsiI TTATAA 1 cut(s) 1218
Psp124BI GAGCTC 1 cut(s) 256
Psp1406I AACGTT 1 cut(s) 324
Psp6I CCWGG 3 cut(s) 110, 663, 709
PspCI CACGTG 2 cut(s) 99, 527
PspGI CCWGG 3 cut(s) 110, 663, 709
PspN4I GGNNCC 7 cut(s) 116, 217, 772, 910, 968, 1277, 1932
PspOMI GGGCCC 1 cut(s) 770
PspPI GGNCC 6 cut(s) 369, 667, 770, 771, 1276, 1931
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 738
RsaI GTAC 2 cut(s) 632, 1015
RsaNI GTAC 2 cut(s) 631, 1014
SacI GAGCTC 1 cut(s) 256
SalI GTCGAC 1 cut(s) 1864
SapI GCTCTTC 1 cut(s) 1135
SaqAI TTAA 5 cut(s) 408, 416, 1056, 1520, 1632
SatI GCNGC 4 cut(s) 243, 538, 616, 619
Sau3AI GATC 8 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109
Sau96I GGNCC 6 cut(s) 369, 667, 770, 771, 1276, 1931
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 5 cut(s) 112, 665, 711, 883, 979
SduI GDGCHC 5 cut(s) 256, 474, 774, 1564, 1900
SfaNI GCATC 4 cut(s) 379, 614, 1354, 1794
SfcI CTRYAG 1 cut(s) 1812
SfoI GGCGCC 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 1643
SfuI TTCGAA 2 cut(s) 435, 658
SinI GGWCC 4 cut(s) 369, 667, 1276, 1931
SlaI CTCGAG 1 cut(s) 1643
SmlI CTYRAG 2 cut(s) 727, 1643
SmoI CTYRAG 2 cut(s) 727, 1643
Sse9I AATT 3 cut(s) 426, 532, 1658
SseBI AGGCCT 2 cut(s) 451, 1288
SsiI CCGC 8 cut(s) 242, 468, 538, 618, 780, 815, 1195, 1893
SspDI GGCGCC 1 cut(s) 908
SspMI CTAG 1 cut(s) 1938
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 2 cut(s) 451, 1288
StyD4I CCNGG 5 cut(s) 110, 663, 709, 881, 977
StyI CCWWGG 4 cut(s) 600, 1265, 1283, 1540
TaaI ACNGT 6 cut(s) 359, 671, 959, 1031, 1280, 1316
TaiI ACGT 8 cut(s) 12, 101, 327, 338, 529, 1046, 1538, 1575
TaqI TCGA 7 cut(s) 396, 435, 545, 658, 1099, 1644, 1865
TaqII GACCGA 2 cut(s) 190, 278
TasI AATT 3 cut(s) 426, 532, 1658
TauI GCSGC 3 cut(s) 245, 540, 621
TfiI GAWTC 3 cut(s) 564, 1639, 1952
Tru1I TTAA 5 cut(s) 408, 416, 1056, 1520, 1632
Tru9I TTAA 5 cut(s) 408, 416, 1056, 1520, 1632
TscAI CASTG 6 cut(s) 22, 176, 902, 924, 1321, 1905
TseFI GTSAC 4 cut(s) 745, 1062, 1247, 1421
TseI GCWGC 1 cut(s) 615
Tsp45I GTSAC 4 cut(s) 745, 1062, 1247, 1421
TspDTI ATGAA 5 cut(s) 854, 963, 1592, 1731, 1944
TspGWI ACGGA 3 cut(s) 125, 1160, 1452
TspRI CASTG 6 cut(s) 22, 176, 902, 924, 1321, 1905
Van91I CCANNNNNTGG 1 cut(s) 919
VpaK11BI GGWCC 4 cut(s) 369, 667, 1276, 1931
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 1643
XmiI GTMKAC 2 cut(s) 850, 1865
XspI CTAG 1 cut(s) 1938
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.