Rorug05G0532900

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000644
Physical Location & Seq
Reverse (-)
72102258 .. 72105094
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0532900.1

Sequence Viewer

Length: 447 bp
ATGGCGTCGAAACGAATCTTGAAGGAGCTCAAGGATTTGCAGAAGGACCCGCCTACCTCTTGCAGTGCTGGTCCCGTGGCGGAGGACATGTTCCATTGGCAAGCAACAATTATGGGACCTGCTGATAGCCCATATGCAGGTGGTGTTTTTCTAGTTTCAATTCACTTCCCTCCGGATTATCCATTCAAGCCTCCAAAGGTTGCATTCAGGACCAAGGTATTCCATCCAAACATCAATAGCAATGGAAGCATATGTCTTGATATTCTTAAGGAACAATGGAGCCCAGCACTAACCATTTCCAAGGTGCTGTTATCCATTTGCTCTCTGTTGACAGACCCCAACCCTGATGATCCGCTTGTCCCCGAAATTGCACACATGTACAAGACCGACAGGGCAAAGTATGAAGCCACTGCTCGCAGCTGGACACAAAAGTATGCCATGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

148

Amino Acids

16.46

Weight (kDa)

7.72

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 5.8e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 2 cut(s) 127, 128
AccIII TCCGGA 1 cut(s) 172
AciI CCGC 3 cut(s) 50, 80, 353
AclWI GGATC 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 137
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 87, 375
AgsI TTSAA 3 cut(s) 22, 159, 187
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw21I GWGCWC 1 cut(s) 30
AlwI GGATC 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 46, 71, 116, 210
AvaII GGWCC 4 cut(s) 46, 71, 116, 210
BanII GRGCYC 2 cut(s) 30, 284
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 231
BfaI CTAG 1 cut(s) 152
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 127, 128
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme18I GGWCC 4 cut(s) 46, 71, 116, 210
BmgT120I GGNCC 4 cut(s) 46, 71, 116, 210
BmiI GGNNCC 4 cut(s) 48, 73, 117, 281
BpuEI CTTGAG 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 75, 213, 300, 438
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 247
BseAI TCCGGA 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 75, 213, 300, 438
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 3 cut(s) 57, 129, 344
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 3 cut(s) 57, 129, 344
BsmI GAATGC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 30, 284
Bsp13I TCCGGA 1 cut(s) 172
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 1 cut(s) 349
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 3 cut(s) 50, 80, 353
BspEI TCCGGA 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 48, 73, 117, 281
BspMI ACCTGC 2 cut(s) 127, 128
BspPI GGATC 1 cut(s) 344
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 4 cut(s) 75, 213, 300, 438
BssMI GATC 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 213, 300, 438
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 102, 415
BstDSI CCRYGG 2 cut(s) 75, 438
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 91, 379
BstV1I GCAGC 1 cut(s) 429
BtgI CCRYGG 2 cut(s) 75, 438
BtsCI GGATG 1 cut(s) 223
BtsI GCAGTG 2 cut(s) 70, 408
BtsIMutI CAGTG 2 cut(s) 70, 408
BveI ACCTGC 2 cut(s) 127, 128
Cac8I GCNNGC 2 cut(s) 102, 415
Cfr13I GGNCC 4 cut(s) 46, 71, 116, 210
Csp6I GTAC 1 cut(s) 379
CviAII CATG 3 cut(s) 88, 376, 439
CviJI RGCY 7 cut(s) 28, 129, 190, 282, 407, 420, 444
CviKI_1 RGCY 7 cut(s) 28, 129, 190, 282, 407, 420, 444
CviQI GTAC 1 cut(s) 379
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 213, 300, 438
Eco24I GRGCYC 2 cut(s) 30, 284
Eco47I GGWCC 4 cut(s) 46, 71, 116, 210
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 2 cut(s) 46, 116
EcoT14I CCWWGG 3 cut(s) 213, 300, 438
EcoT38I GRGCYC 2 cut(s) 30, 284
ErhI CCWWGG 3 cut(s) 213, 300, 438
FaeI CATG 3 cut(s) 91, 379, 442
FaqI GGGAC 3 cut(s) 57, 129, 344
FatI CATG 3 cut(s) 87, 375, 438
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 2 cut(s) 133, 251
Fnu4HI GCNGC 1 cut(s) 418
FokI GGATG 1 cut(s) 210
FriOI GRGCYC 2 cut(s) 30, 284
Fsp4HI GCNGC 1 cut(s) 418
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 418
GsaI CCCAGC 1 cut(s) 287
HapII CCGG 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 91, 379, 442
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 15
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 330
Hpy188III TCNNGA 4 cut(s) 19, 173, 208, 257
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 16, 37
HpyCH4V TGCA 5 cut(s) 40, 63, 137, 203, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 91, 379, 442
Kpn2I TCCGGA 1 cut(s) 172
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 2 cut(s) 25, 279
LpnPI CCDG 9 cut(s) 54, 123, 132, 186, 193, 297, 357, 376, 406
Lsp1109I GCAGC 1 cut(s) 429
MaeI CTAG 1 cut(s) 152
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MhlI GDGCHC 2 cut(s) 30, 284
MluCI AATT 3 cut(s) 108, 159, 366
MnlI CCTC 4 cut(s) 67, 76, 180, 201
MroI TCCGGA 1 cut(s) 172
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 420
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 246
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 2 cut(s) 133, 251
NdeII GATC 1 cut(s) 349
NlaIII CATG 3 cut(s) 91, 379, 442
NlaIV GGNNCC 4 cut(s) 48, 73, 117, 281
NspI RCATGY 2 cut(s) 91, 379
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 2 cut(s) 87, 375
PctI GAATGC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 419
PpuMI RGGWCCY 2 cut(s) 46, 116
PscI ACATGT 2 cut(s) 87, 375
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 2 cut(s) 46, 116
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 4 cut(s) 48, 73, 117, 281
PspPI GGNCC 4 cut(s) 46, 71, 116, 210
PspPPI RGGWCCY 2 cut(s) 46, 116
PvuII CAGCTG 1 cut(s) 420
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 4 cut(s) 46, 71, 116, 210
SduI GDGCHC 2 cut(s) 30, 284
SetI ASST 8 cut(s) 30, 59, 121, 142, 201, 219, 306, 422
SinI GGWCC 4 cut(s) 46, 71, 116, 210
SmlI CTYRAG 2 cut(s) 29, 266
SmoI CTYRAG 2 cut(s) 29, 266
Sse9I AATT 3 cut(s) 108, 159, 366
SsiI CCGC 3 cut(s) 50, 80, 353
SspMI CTAG 1 cut(s) 152
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 3 cut(s) 213, 300, 438
TaqI TCGA 1 cut(s) 8
TaqII GACCGA 1 cut(s) 401
TasI AATT 3 cut(s) 108, 159, 366
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 70, 415
TseI GCWGC 1 cut(s) 417
TspDTI ATGAA 1 cut(s) 417
TspRI CASTG 2 cut(s) 70, 415
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 4 cut(s) 46, 71, 116, 210
XceI RCATGY 2 cut(s) 91, 379
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.