Rw6G004440

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000644
Physical Location & Seq
Reverse (-)
7103539 .. 7110397
6859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G004440.1

Sequence Viewer

Length: 2079 bp
ATGTCTGACGTCATCACCGGCCTTCCCTGCAACTCCGACGGCCTCTGCATGGTGTGCAACCAAAAGCCGTCGCCGGAGGACCTGTTGTCTTGCACCACTTGCGCCTCACCCTGGCACCTGACTTGCCTCTCCGTCCGTCCCGAAACCTCAGCCGACGCTCTTGTGTGGCAGTGTCCTGACTGCACTTCTCCGACGGCTTCGGTAGGTGCCGGAGGAGCAGGAAGCTCCTGCGATCTGGTGGCGGCCATCCGAGCTCTGGAGACAGATGAGTCGTTGAGTGAGGCTGAGAAGGCGAAGAAACGGCAGGAGCTGATGTCCAAAAACGTTTTAGCAACGTCCGATGGCGACGGCAACAACCGTGTGAAGAGGACCAACGGCAGTCGTAATGATGCCCTCGATATTCTCATTAAGAGCTTAACCTGTTCAATTTGCTTCGAGCTTCCGGAGAGGCCTGTTACGACACCTTGCGGGCACAACTTCTGCCTCAAATGCTTTGAGAAATGGGTGGCGCAGGGTAAACTTACATGTGCAAATTGCCGCCAATCGATCCCCTCCACTCTGAGAATCAACTCTAATCTTGTGGGAAGCATTCGTATTGCCAAGGCATCACAACGCAGTGGCTCAGGGGAGTACCTACCTCAGTTGGCTCATCGCATTAGAAACCAGGACCGTCCAGACAAAGCATTTACAACCGAGCGAGCCAAGAAGCCTGGGAAAGCTAATGCCTCAAGTGGAAGGATCTTTGTGACATCAGCTCCAGATCATTTTGGGCCCATAACCGCCGAATATGATCCTGAGAAGAATAAGGGTGTGGCGGTGGGGGAGACTTGGGGGCATAGGATTGAATGTAGACAATGGGGTATTCATCGCCCTATGGTTTCCGGCATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCATTTACACCGGGAGTGGTGGCAAGGATTTGAGTGGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGACGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTGAATGGTTATCCTGTTCGAGTAGTGAGATCGCACAAGGAGACACGCTCTTCGTATGCGCCTACGGAGGTGGGACTGCGATATGATGGGGTTTACAGGATTGAAAAATGTTGGCGGAAAGATGGAACACAGGGTTATAAGGTTTGCAGATATCTTTTTGTAAGATGTGACAACGAACCTGCGCCTTGGACGAGTGATGATCATGGGGACCGTCCAAGGCCTCTGCCAGATATTGCAGAGTTGAACAGTGCCATTGATATAACAAATACTAAGGGTTCTCCTTCTTGGGACTATGATGCAGAAAAAGCGTGCTGGATGTGGAAAAAGCCTCGACCACTTAGCAGACAACCGGTGGATAGGGGAGAACCAGATGGGAAGAAAATTAGGAGAGTTAGACGGCAGGAACAGAATGCGAAAGAAAAAGTTCTCAAAGGGTTTAGGTGTGATAAATGCCAGAAGATTATGACCGAACCAGTTACAACACCATGTGGTCATAACTTCTGCAAAGCTTGTCTGCAAGATACCTTTGCTGGTATAAGTTTAATAAAACAGAGGACGTGCCAAGGCGGAAGAACATTGCGAGCACAAAAGAACGTCATGAAATGCCCAAAGTGTCCACATGACATATCTGACTTTCTTCAAAATCCACAGGTTAATAAGAATCTCGAGAACGCCATAGAATTACTCCAGCAGAAAGAGGAAGATGTTGCAGAGGAAGTGGAAGACGCTGAGGAAGATGAAGATGATGAAGAAGAGGAAGAAGAGGAAGAAGCTGAAGAAACTTACAATGTCGGTGAAGAGAGTGAAGCCGAAGACAAAGGAAGCATCCCACCAATACCTCAGACTGAAACTACAGAGCAACAAACCTGTGAGCAAAAGAAACCAAGTCATAAGCGAAATAGGTCGAGCAATGGAGACAACGTGTTGGATGGCGGAGCACTGACCAAGAGAAGCAAGAGGAGTAAAGTGGGAGCAGCTAGTAGTCTGATTGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

692

Amino Acids

76.73

Weight (kDa)

6.69

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 16 - 61 1.7e-06 PHD-finger
zf-C3HC4_3 PF13920 139 - 184 1.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 140 - 180 1e-07 Ring finger domain
zf-RING_UBOX PF13445 141 - 176 3.9e-08 RING-type zinc-finger
zf-C3HC4 PF00097 141 - 179 1.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 141 - 179 3.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 141 - 179 2.7e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 268 - 418 7.4e-48 SAD/SRA domain
zf-RING_UBOX PF13445 508 - 538 1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
AasI GACNNNNNNGTC 1 cut(s) 268
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 1267
AccB1I GGYRCC 3 cut(s) 114, 206, 908
AccB7I CCANNNNNTGG 1 cut(s) 919
AccI GTMKAC 1 cut(s) 850
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 8 cut(s) 242, 468, 538, 780, 815, 1195, 1647, 2013
AclI AACGTT 1 cut(s) 324
AclWI GGATC 3 cut(s) 541, 746, 785
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 1875
AcyI GRCGYC 2 cut(s) 9, 909
AdeI CACNNNGTG 1 cut(s) 1568
AfaI GTAC 2 cut(s) 632, 1015
AfiI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
AflIII ACRYGT 2 cut(s) 524, 2001
AgeI ACCGGT 1 cut(s) 1429
AgsI TTSAA 5 cut(s) 426, 845, 1184, 1324, 1721
AhdI GACNNNNNGTC 1 cut(s) 85
AjiI CACGTC 1 cut(s) 1638
AjnI CCWGG 3 cut(s) 110, 663, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
Alw21I GWGCWC 3 cut(s) 256, 1666, 2020
Alw26I GTCTC 4 cut(s) 254, 818, 1115, 1989
AlwI GGATC 3 cut(s) 541, 746, 785
AlwNI CAGNNNCTG 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 1745
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 6 cut(s) 19, 40, 243, 449, 770, 1298
ApaI GGGCCC 1 cut(s) 774
ApeKI GCWGC 1 cut(s) 2054
ArsI GACNNNNNNTTYG 2 cut(s) 1114, 1146
AsiGI ACCGGT 1 cut(s) 1429
Asp700I GAANNNNTTC 1 cut(s) 1503
AspLEI GCGC 5 cut(s) 104, 511, 911, 1141, 1264
AspS9I GGNCC 6 cut(s) 79, 369, 667, 770, 771, 1288
AsuC2I CCSGG 1 cut(s) 979
AsuHPI GGTGA 4 cut(s) 7, 99, 971, 1886
AvaI CYCGRG 1 cut(s) 1745
AvaII GGWCC 4 cut(s) 79, 369, 667, 1288
BaeGI GKGCMC 2 cut(s) 474, 774
BaeI ACNNNNGTAYC 2 cut(s) 997, 1030
BanI GGYRCC 3 cut(s) 114, 206, 908
BanII GRGCYC 2 cut(s) 256, 774
BbsI GAAGAC 2 cut(s) 1809, 1899
Bbv12I GWGCWC 3 cut(s) 256, 1666, 2020
BbvCI CCTCAGC 2 cut(s) 148, 1809
BbvI GCAGC 1 cut(s) 2066
BccI CCATC 6 cut(s) 254, 335, 1160, 1196, 1445, 2003
BceAI ACGGC 7 cut(s) 52, 55, 210, 317, 364, 391, 1493
BciT130I CCWGG 3 cut(s) 112, 665, 711
BclI TGATCA 1 cut(s) 1279
BcnI CCSGG 1 cut(s) 979
BcoDI GTCTC 4 cut(s) 254, 818, 1115, 1989
BfaI CTAG 1 cut(s) 2058
BfmI CTRYAG 1 cut(s) 1932
BfoI RGCGCY 1 cut(s) 912
BfuAI ACCTGC 1 cut(s) 1267
BisI GCNGC 3 cut(s) 243, 538, 2055
BlsI GCNGC 3 cut(s) 244, 539, 2056
Bme1390I CCNGG 4 cut(s) 112, 665, 711, 979
Bme18I GGWCC 4 cut(s) 79, 369, 667, 1288
BmeRI GACNNNNNGTC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 1745
BmgBI CACGTC 1 cut(s) 1638
BmgT120I GGNCC 6 cut(s) 79, 369, 667, 770, 771, 1288
BmiI GGNNCC 5 cut(s) 116, 208, 772, 910, 1289
BmrFI CCNGG 4 cut(s) 112, 665, 711, 979
BmsI GCATC 4 cut(s) 379, 614, 1366, 1914
BpiI GAAGAC 2 cut(s) 1809, 1899
BplI GAGNNNNNCTC 2 cut(s) 1112, 1144
BpmI CTGGAG 3 cut(s) 278, 741, 1751
Bpu10I CCTNAGC 3 cut(s) 148, 622, 1809
BpuEI CTTGAG 1 cut(s) 712
BpuMI CCSGG 1 cut(s) 979
Bsa29I ATCGAT 1 cut(s) 545
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 9, 909
BsaJI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1295, 1642
BsaWI WCCGGW 2 cut(s) 442, 1429
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 299, 329, 739, 769, 1986, 2016
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
Bse118I RCCGGY 2 cut(s) 17, 1429
Bse1I ACTGG 1 cut(s) 1553
Bse3DI GCAATG 2 cut(s) 1655, 1996
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 3 cut(s) 112, 665, 711
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1295, 1642
BseGI GGATG 4 cut(s) 246, 1401, 1905, 2014
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
BseMI GCAATG 2 cut(s) 1655, 1996
BseNI ACTGG 1 cut(s) 1553
BseRI GAGGAG 2 cut(s) 228, 2053
BseSI GKGCMC 2 cut(s) 474, 774
BseXI GCAGC 1 cut(s) 2066
BsgI GTGCAG 1 cut(s) 166
BshFI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1300
BshNI GGYRCC 3 cut(s) 114, 206, 908
BshTI ACCGGT 1 cut(s) 1429
BshVI ATCGAT 1 cut(s) 545
BsiHKAI GWGCWC 3 cut(s) 256, 1666, 2020
BsiHKCI CYCGRG 1 cut(s) 1745
BsiSI CCGG 7 cut(s) 18, 74, 210, 443, 882, 978, 1430
BslFI GGGAC 4 cut(s) 123, 1167, 1301, 1382
BslI CCNNNNNNNGG 4 cut(s) 49, 111, 256, 919
BsmAI GTCTC 4 cut(s) 254, 818, 1115, 1989
BsmFI GGGAC 4 cut(s) 123, 1167, 1301, 1382
BsmI GAATGC 2 cut(s) 588, 1495
BsnI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1300
BsoBI CYCGRG 1 cut(s) 1745
Bsp120I GGGCCC 1 cut(s) 770
Bsp1286I GDGCHC 5 cut(s) 256, 474, 774, 1666, 2020
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
BspACI CCGC 8 cut(s) 242, 468, 538, 780, 815, 1195, 1647, 2013
BspANI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1300
BspDI ATCGAT 1 cut(s) 545
BspEI TCCGGA 1 cut(s) 442
BspHI TCATGA 2 cut(s) 1677, 2075
BspLI GGNNCC 5 cut(s) 116, 208, 772, 910, 1289
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 3 cut(s) 541, 746, 785
BspQI GCTCTTC 1 cut(s) 1135
BspT107I GGYRCC 3 cut(s) 114, 206, 908
BsrDI GCAATG 2 cut(s) 1655, 1996
BsrFI RCCGGY 2 cut(s) 17, 1429
BsrI ACTGG 1 cut(s) 1553
BssAI RCCGGY 2 cut(s) 17, 1429
BssECI CCNNGG 6 cut(s) 110, 600, 710, 1265, 1295, 1642
BssMI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
BssNI GRCGYC 2 cut(s) 9, 909
BssT1I CCWWGG 4 cut(s) 600, 1265, 1295, 1642
Bst2UI CCWGG 3 cut(s) 112, 665, 711
Bst4CI ACNGT 6 cut(s) 359, 671, 959, 1031, 1292, 1328
Bst6I CTCTTC 5 cut(s) 359, 1135, 1827, 1836, 1872
BstACI GRCGYC 2 cut(s) 9, 909
BstAPI GCANNNNNTGC 2 cut(s) 54, 99
BstC8I GCNNGC 4 cut(s) 470, 699, 1390, 1662
BstF5I GGATG 4 cut(s) 246, 1401, 1905, 2014
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 5 cut(s) 104, 511, 911, 1141, 1264
BstKTI GATC 9 cut(s) 235, 549, 741, 763, 793, 955, 1039, 1112, 1282
BstMAI GTCTC 4 cut(s) 254, 818, 1115, 1989
BstMBI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
BstMWI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1385
BstNI CCWGG 3 cut(s) 112, 665, 711
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 4 cut(s) 110, 663, 709, 977
BstSFI CTRYAG 1 cut(s) 1932
BstSLI GKGCMC 2 cut(s) 474, 774
BstV1I GCAGC 1 cut(s) 2066
BstV2I GAAGAC 2 cut(s) 1809, 1899
BstX2I RGATCY 1 cut(s) 738
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 6 cut(s) 21, 42, 245, 451, 772, 1300
BsuTUI ATCGAT 1 cut(s) 545
BtgZI GCGATG 2 cut(s) 635, 851
BtrI CACGTC 1 cut(s) 1638
BtsCI GGATG 4 cut(s) 246, 1401, 1905, 2014
BtsI GCAGTG 3 cut(s) 176, 622, 902
BtsIMutI CAGTG 6 cut(s) 176, 622, 902, 924, 1333, 2018
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 4 cut(s) 470, 699, 1390, 1662
CaiI CAGNNNCTG 1 cut(s) 310
CciI TCATGA 2 cut(s) 1677, 2075
CfoI GCGC 5 cut(s) 104, 511, 911, 1141, 1264
Cfr10I RCCGGY 2 cut(s) 17, 1429
Cfr13I GGNCC 6 cut(s) 79, 369, 667, 770, 771, 1288
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 2 cut(s) 164, 1814
Csp6I GTAC 2 cut(s) 631, 1014
CspAI ACCGGT 1 cut(s) 1429
CviAII CATG 7 cut(s) 49, 525, 1283, 1566, 1678, 1700, 2076
CviQI GTAC 2 cut(s) 631, 1014
DinI GGCGCC 1 cut(s) 910
DpnI GATC 9 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1111, 1281
DpnII GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
DraIII CACNNNGTG 1 cut(s) 1568
DrdI GACNNNNNNGTC 1 cut(s) 268
DriI GACNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 5 cut(s) 359, 1135, 1827, 1836, 1872
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 5 cut(s) 359, 1135, 1827, 1836, 1872
EciI GGCGGA 3 cut(s) 1210, 1662, 2028
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 4 cut(s) 600, 1265, 1295, 1642
Eco147I AGGCCT 2 cut(s) 451, 1300
Eco24I GRGCYC 2 cut(s) 256, 774
Eco32I GATATC 1 cut(s) 1232
Eco47I GGWCC 4 cut(s) 79, 369, 667, 1288
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 1875
Eco88I CYCGRG 1 cut(s) 1745
EcoICRI GAGCTC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 3 cut(s) 110, 663, 709
EcoRV GATATC 1 cut(s) 1232
EcoT14I CCWWGG 4 cut(s) 600, 1265, 1295, 1642
EcoT38I GRGCYC 2 cut(s) 256, 774
EgeI GGCGCC 1 cut(s) 910
EheI GGCGCC 1 cut(s) 910
ErhI CCWWGG 4 cut(s) 600, 1265, 1295, 1642
FaeI CATG 7 cut(s) 52, 528, 1286, 1569, 1681, 1703, 2079
FaqI GGGAC 4 cut(s) 123, 1167, 1301, 1382
FatI CATG 7 cut(s) 48, 524, 1282, 1565, 1677, 1699, 2075
FauI CCCGC 1 cut(s) 461
FbaI TGATCA 1 cut(s) 1279
FblI GTMKAC 1 cut(s) 850
Fnu4HI GCNGC 3 cut(s) 243, 538, 2055
FokI GGATG 4 cut(s) 233, 1408, 1892, 2021
FriOI GRGCYC 2 cut(s) 256, 774
Fsp4HI GCNGC 3 cut(s) 243, 538, 2055
FspBI CTAG 1 cut(s) 2058
GlaI GCGC 5 cut(s) 103, 510, 910, 1140, 1263
GluI GCNGC 3 cut(s) 243, 538, 2055
GsuI CTGGAG 3 cut(s) 278, 741, 1751
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 6 cut(s) 21, 42, 245, 451, 772, 1300
HapII CCGG 7 cut(s) 18, 74, 210, 443, 882, 978, 1430
HgaI GACGC 2 cut(s) 164, 1814
HhaI GCGC 5 cut(s) 104, 511, 911, 1141, 1264
Hin1I GRCGYC 2 cut(s) 9, 909
Hin1II CATG 7 cut(s) 52, 528, 1286, 1569, 1681, 1703, 2079
Hin6I GCGC 5 cut(s) 102, 509, 909, 1139, 1262
HinP1I GCGC 5 cut(s) 102, 509, 909, 1139, 1262
HindIII AAGCTT 2 cut(s) 1052, 1587
HinfI GANTC 4 cut(s) 269, 564, 1741, 2072
HpaII CCGG 7 cut(s) 18, 74, 210, 443, 882, 978, 1430
HphI GGTGA 4 cut(s) 7, 99, 971, 1886
Hpy166II GTNNAC 5 cut(s) 518, 851, 1047, 1174, 1697
Hpy8I GTNNAC 5 cut(s) 518, 851, 1047, 1174, 1697
Hpy99I CGWCG 5 cut(s) 41, 73, 158, 196, 350
HpyAV CCTTC 4 cut(s) 32, 283, 729, 1371
HpyCH4III ACNGT 6 cut(s) 359, 671, 959, 1031, 1292, 1328
HpyCH4IV ACGT 7 cut(s) 9, 324, 335, 1043, 1637, 1674, 2001
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1385
HpySE526I ACGT 7 cut(s) 9, 324, 335, 1043, 1637, 1674, 2001
Hsp92I GRCGYC 2 cut(s) 9, 909
Hsp92II CATG 7 cut(s) 52, 528, 1286, 1569, 1681, 1703, 2079
HspAI GCGC 5 cut(s) 102, 509, 909, 1139, 1262
KasI GGCGCC 1 cut(s) 908
Kpn2I TCCGGA 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 1279
Kzo9I GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
LguI GCTCTTC 1 cut(s) 1135
LmnI GCTCC 6 cut(s) 215, 230, 307, 760, 2015, 2051
Lsp1109I GCAGC 1 cut(s) 2066
LweI GCATC 4 cut(s) 379, 614, 1366, 1914
MaeI CTAG 1 cut(s) 2058
MaeII ACGT 7 cut(s) 9, 324, 335, 1043, 1637, 1674, 2001
MaeIII GTNAC 5 cut(s) 454, 745, 1062, 1247, 1555
MalI GATC 9 cut(s) 234, 548, 740, 762, 792, 954, 1038, 1111, 1281
MboI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 5 cut(s) 256, 474, 774, 1666, 2020
MluCI AATT 4 cut(s) 426, 532, 1461, 1760
Mly113I GGCGCC 1 cut(s) 909
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 3 cut(s) 60, 215, 1986
MroI TCCGGA 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 1503
MseI TTAA 5 cut(s) 408, 416, 1056, 1622, 1734
MspI CCGG 7 cut(s) 18, 74, 210, 443, 882, 978, 1430
MspR9I CCNGG 4 cut(s) 112, 665, 711, 979
Mva1269I GAATGC 2 cut(s) 588, 1495
MvaI CCWGG 3 cut(s) 112, 665, 711
MwoI GCNNNNNNNGC 9 cut(s) 27, 54, 99, 215, 251, 290, 489, 906, 1385
NarI GGCGCC 1 cut(s) 909
NciI CCSGG 1 cut(s) 979
NdeII GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
NlaIII CATG 7 cut(s) 52, 528, 1286, 1569, 1681, 1703, 2079
NlaIV GGNNCC 5 cut(s) 116, 208, 772, 910, 1289
NmuCI GTSAC 3 cut(s) 745, 1062, 1247
NspI RCATGY 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 1745
PagI TCATGA 2 cut(s) 1677, 2075
PceI AGGCCT 2 cut(s) 451, 1300
PciI ACATGT 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 1135
PctI GAATGC 2 cut(s) 588, 1495
PdmI GAANNNNTTC 1 cut(s) 1503
PfeI GAWTC 3 cut(s) 564, 1741, 2072
PflMI CCANNNNNTGG 1 cut(s) 919
PinAI ACCGGT 1 cut(s) 1429
PkrI GCNGC 3 cut(s) 244, 539, 2056
PleI GAGTC 1 cut(s) 277
PluTI GGCGCC 1 cut(s) 912
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 1 cut(s) 524
PsiI TTATAA 1 cut(s) 1218
Psp124BI GAGCTC 1 cut(s) 256
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 3 cut(s) 110, 663, 709
PspGI CCWGG 3 cut(s) 110, 663, 709
PspN4I GGNNCC 5 cut(s) 116, 208, 772, 910, 1289
PspOMI GGGCCC 1 cut(s) 770
PspPI GGNCC 6 cut(s) 79, 369, 667, 770, 771, 1288
PspPPI RGGWCCY 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 738
RsaI GTAC 2 cut(s) 632, 1015
RsaNI GTAC 2 cut(s) 631, 1014
SacI GAGCTC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 1135
SaqAI TTAA 5 cut(s) 408, 416, 1056, 1622, 1734
SatI GCNGC 3 cut(s) 243, 538, 2055
Sau3AI GATC 9 cut(s) 232, 546, 738, 760, 790, 952, 1036, 1109, 1279
Sau96I GGNCC 6 cut(s) 79, 369, 667, 770, 771, 1288
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 4 cut(s) 112, 665, 711, 979
SduI GDGCHC 5 cut(s) 256, 474, 774, 1666, 2020
SfaNI GCATC 4 cut(s) 379, 614, 1366, 1914
SfcI CTRYAG 1 cut(s) 1932
SfoI GGCGCC 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 1745
SinI GGWCC 4 cut(s) 79, 369, 667, 1288
SlaI CTCGAG 1 cut(s) 1745
SmlI CTYRAG 2 cut(s) 727, 1745
SmoI CTYRAG 2 cut(s) 727, 1745
Sse9I AATT 4 cut(s) 426, 532, 1461, 1760
SseBI AGGCCT 2 cut(s) 451, 1300
SsiI CCGC 8 cut(s) 242, 468, 538, 780, 815, 1195, 1647, 2013
SspDI GGCGCC 1 cut(s) 908
SspMI CTAG 1 cut(s) 2058
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 2 cut(s) 451, 1300
StyD4I CCNGG 4 cut(s) 110, 663, 709, 977
StyI CCWWGG 4 cut(s) 600, 1265, 1295, 1642
TaaI ACNGT 6 cut(s) 359, 671, 959, 1031, 1292, 1328
TaiI ACGT 7 cut(s) 12, 327, 338, 1046, 1640, 1677, 2004
TaqI TCGA 7 cut(s) 396, 435, 545, 1099, 1411, 1746, 1985
TaqII GACCGA 1 cut(s) 1562
TasI AATT 4 cut(s) 426, 532, 1461, 1760
TauI GCSGC 2 cut(s) 245, 540
TfiI GAWTC 3 cut(s) 564, 1741, 2072
Tru1I TTAA 5 cut(s) 408, 416, 1056, 1622, 1734
Tru9I TTAA 5 cut(s) 408, 416, 1056, 1622, 1734
TscAI CASTG 6 cut(s) 176, 622, 902, 924, 1333, 2025
TseFI GTSAC 3 cut(s) 745, 1062, 1247
TseI GCWGC 1 cut(s) 2054
Tsp45I GTSAC 3 cut(s) 745, 1062, 1247
TspDTI ATGAA 7 cut(s) 854, 958, 963, 1694, 1833, 1842, 2064
TspGWI ACGGA 3 cut(s) 121, 125, 1160
TspRI CASTG 6 cut(s) 176, 622, 902, 924, 1333, 2025
Van91I CCANNNNNTGG 1 cut(s) 919
VpaK11BI GGWCC 4 cut(s) 79, 369, 667, 1288
XceI RCATGY 1 cut(s) 528
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 1745
XmiI GTMKAC 1 cut(s) 850
XmnI GAANNNNTTC 1 cut(s) 1503
XspI CTAG 1 cut(s) 2058
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.