RchiOBHm_Chr7g0213481

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000644
Physical Location & Seq
Reverse (-)
30589494 .. 30595275
5782 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19109

Sequence Viewer

Length: 2649 bp
ATGGCGCACGTTAGCCAACTGCCGTGCGACGGCGACGGCGTATGCATGCTCTGCAAGGCGAAGCCGTCCGACGAGGAGAAGCTGACCTGCAAGACCTGCGTCACCCCCTGGCACGCCTCCTGCCTCGCTCCCGGCTCGCGCCCCGAAACCTTGGCCTCGACGGCTCAGTGGGAGTGCCCGGACTGCACCTCGATCTCAGGCGGGGCTCCGGCTGTGAAGTCCGGCGAGTCCGGGGACTTGATAGCCAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGACAGGAGCTGATGAGCGGAAAGGCGGAGGAGGAGGAGGAGGAGGAGGAAAAGAAAGGAGCGAAGAAGGACGTGTTGGATGTTCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACGACACCATGCGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTCAAAAGATGGCAAGTCAACCCCGAATCAACTCTTCCCTTGTAATTGCCATACGTATGGCTAAGCTTTCAAAATCACATGTTACTGGGGTGGCTGCCAAGGTATATCATTACATACAAAACCAAGACAGGCCTGACAAAGCATACACTACTGAGCGAGCACAAAAGACTGGAAAAGCTAATGCTGCAAGTGGCAAGATATTTGTTACAATTCCTTCAGATCATTTTGGACCTATACCTGCTGAAAATGATCCCGAGAGAAATCAAGGTGTCATGGTTGGGGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACATTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGCGCACAGTCTGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAATGGTTCTTATACACAGGAAGTGGCGGAAGAGATTTGAGTGGGAACAAGCGAACTAATAAGGACCAGTCCTTTGACCAGACTTTTGAAAAGTATAACAAGGCGCTCCAAGTTAGTTGCCAGAAAGGCTATCCTGTAAGGGTGGTAAGATCGCACAAGGAGAAGCGCTCCGCATATGCCCCTGAGAAGGGGTTGCGGTATGATGGAGTTTATAGAATTGAGAAATGTTGGAGAAAGGTGGGAGTCCAGGGGTTTAAAGTCTGCCGGTATCTTTTTGTCAGATGTGACAACGAACCTGCACCATGGACAAGTGATGAGCATGGAGATCGGCCTAGACCACTACCAGCAATACCCGAGCTGGCAAAAGCCACAAACATAACAGAGAGGAATGCAAGTCCATCATGGGACTTTGAGGAGAAAGATGGCCGCTGGAAATGGATAAAGGCACCACCTATCAGCAAAAAACCTGTTCAAACATTAGATCCTGCTGATCGAAAGAGGTCAAGGAAAGCAATGAGGAGAGCACAGAACATGACTGTACGGGAGAGGCTCTTGAAAGAATTTAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCCGTTACCACATCATGTGGTCATAATTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGCGGGTAAGAGTGTGGTGACAGAAAGAAACAGAGGTGGAAGATCCCTTCGAACTCAAAGGAATGTACTGACCTGCCCTTCCTGTCCCACCGATGTCACTGATTTTGTTCAAAATCCCCAGGTGAACACAGAGATAATGAAAGCAATTGAGTCACTGAAAGAAGAGGAAGCGAATGTAGAATCAGCTGATGAATCAAGTGAAGAAATGGACATTCCTGACAATGAAAATAGCAGAGAAATGCCAGAAGAAGCAGCTGTGGTAGATGGTGAGCAGACTTCTCCAACAAAATGCAACCCCAAAGGCAGTCCCAAACGACTGAAAGTTGATACTGGTAATGACTCACCAAAGAGTGATATTGTGGAGGTAAATGAAATTTCTGCTGTCCAGACTAATGGTGTCAATGGAGATACTGAGATGGAAATTGCCAAGGATAGCAATGAGGTACAGGCTCCAGTTGCGAAACAAGGGAAAAAAACTGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAACAAAGAAGGCTAAGGAAGCTGTGAGTGAAGAGAACGGCTCACCATCAAGTAATGAAGTTTCTGCTGTCCACACTAATGATGTCAATGGAGATTCTGATTTAGATATAGCCAAGGAGACCAATTCTGAGATGGAAGTTGCCAAGGAAAGTAATGAGGTACAGGCTCCAGTTGTGAAACGTGGGAAGAAAAATGCTGGTCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGTTAAGGAAGCTGTGAGTGAAGATAACGGCTCACCATCAAGTAATGAAGTTTCTGCTATCCAGACTAATGGTGTTAATGGAGATTCTGATTTAGATATAGCCAAGGAGAGCAATGAGTTACAGGCTCCAGTGGCAAAACGTGGGAACAAAGCTGCTGCTGGTCTAGCTGTTGAAGGTGTTGGATCAAGGACCAGGGGAAGGAAGAAGGCTAAGGTAGCAGCGAGTGAAGAGAATGGTTCACCATCTAGTCCTCTCCATGTGGATGTGGATTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

882

Amino Acids

96.17

Weight (kDa)

7.82

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 135 - 179 4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 135 - 175 5.3e-06 Ring finger domain
zf-C3HC4 PF00097 136 - 174 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 136 - 174 3.4e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 266 - 415 5.5e-50 SAD/SRA domain
zf-RING_UBOX PF13445 508 - 532 1.7e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 508 - 534 3.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 5 cut(s) 95, 104, 781, 1258, 1688
Acc65I GGTACC 1 cut(s) 930
AccB1I GGYRCC 2 cut(s) 930, 1399
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 844
AccII CGCG 1 cut(s) 139
AclWI GGATC 6 cut(s) 244, 779, 954, 1430, 1644, 2560
AcoI YGGCCR 4 cut(s) 454, 1378, 2098, 2332
AcsI RAATTY 2 cut(s) 1514, 1980
AcuI CTGAAG 2 cut(s) 491, 735
AfaI GTAC 5 cut(s) 932, 1494, 1674, 2052, 2286
AfeI AGCGCT 1 cut(s) 1121
AfiI CCNNNNNNNGG 6 cut(s) 29, 487, 1141, 1142, 2361, 2568
AflIII ACRYGT 2 cut(s) 375, 613
AgsI TTSAA 8 cut(s) 403, 606, 1043, 1427, 1510, 1598, 1718, 2543
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 8 cut(s) 107, 252, 1200, 1536, 1725, 2120, 2354, 2561
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
Alw21I GWGCWC 4 cut(s) 529, 697, 859, 1480
Alw26I GTCTC 2 cut(s) 427, 2237
Alw44I GTGCAC 1 cut(s) 855
AlwI GGATC 6 cut(s) 244, 779, 954, 1430, 1644, 2560
AlwNI CAGNNNCTG 1 cut(s) 313
Ama87I CYCGRG 2 cut(s) 788, 1307
Aor51HI AGCGCT 1 cut(s) 1121
AoxI GGCC 7 cut(s) 153, 454, 665, 1283, 1378, 2098, 2332
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 9 cut(s) 421, 424, 629, 719, 1521, 1859, 2522, 2525, 2588
ApoI RAATTY 2 cut(s) 1514, 1980
ArsI GACNNNNNNTTYG 4 cut(s) 1010, 1022, 1042, 1054
Asp700I GAANNNNTTC 1 cut(s) 476
Asp718I GGTACC 1 cut(s) 930
AspLEI GCGC 5 cut(s) 7, 141, 905, 1060, 1122
AspS9I GGNCC 3 cut(s) 764, 1018, 2559
AsuC2I CCSGG 3 cut(s) 132, 179, 232
AsuII TTCGAA 1 cut(s) 1657
AvaI CYCGRG 2 cut(s) 788, 1307
AvaII GGWCC 3 cut(s) 764, 1018, 2559
BaeGI GKGCMC 2 cut(s) 179, 859
BalI TGGCCA 2 cut(s) 2100, 2334
BanI GGYRCC 2 cut(s) 930, 1399
BanII GRGCYC 1 cut(s) 208
Bbv12I GWGCWC 4 cut(s) 529, 697, 859, 1480
BbvI GCAGC 9 cut(s) 411, 433, 616, 706, 1508, 1871, 2509, 2512, 2600
BceAI ACGGC 8 cut(s) 7, 46, 49, 52, 177, 507, 2179, 2413
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 8 cut(s) 109, 254, 1202, 1538, 1727, 2122, 2356, 2563
BcnI CCSGG 3 cut(s) 132, 179, 232
BcoDI GTCTC 2 cut(s) 427, 2237
BfaI CTAG 3 cut(s) 1287, 2534, 2616
BfoI RGCGCY 2 cut(s) 1061, 1123
BfuAI ACCTGC 5 cut(s) 95, 104, 781, 1258, 1688
BglI GCCNNNNNGGC 2 cut(s) 161, 1080
BlpI GCTNAGC 1 cut(s) 597
Bme18I GGWCC 3 cut(s) 764, 1018, 2559
BmeT110I CYCGRG 2 cut(s) 788, 1307
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 3 cut(s) 764, 1018, 2559
BmiI GGNNCC 6 cut(s) 207, 932, 1401, 2058, 2292, 2496
BmrI ACTGGG 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 630
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 1106, 1138, 2150, 2182
BpmI CTGGAG 3 cut(s) 2043, 2277, 2481
Bpu10I CCTNAGC 2 cut(s) 2139, 2580
Bpu1102I GCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 1657
BpuMI CCSGG 3 cut(s) 132, 179, 232
BsaAI YACGTR 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 945
BsaI GGTCTC 2 cut(s) 427, 2237
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 487, 1141, 1142, 2361, 2568
Bse118I RCCGGY 1 cut(s) 1218
Bse1I ACTGG 7 cut(s) 625, 709, 1021, 1942, 2060, 2294, 2498
Bse3DI GCAATG 3 cut(s) 1473, 2050, 2488
Bse8I GATNNNNATC 1 cut(s) 945
BseBI CCWGG 8 cut(s) 109, 254, 1202, 1538, 1727, 2122, 2356, 2563
BseGI GGATG 3 cut(s) 388, 397, 2638
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 6 cut(s) 29, 487, 1141, 1142, 2361, 2568
BseMI GCAATG 3 cut(s) 1473, 2050, 2488
BseMII CTCAG 6 cut(s) 179, 210, 678, 1128, 2010, 2244
BseNI ACTGG 7 cut(s) 625, 709, 1021, 1942, 2060, 2294, 2498
BseSI GKGCMC 2 cut(s) 179, 859
BseXI GCAGC 9 cut(s) 411, 433, 616, 706, 1508, 1871, 2509, 2512, 2600
BsgI GTGCAG 2 cut(s) 169, 1236
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 155, 456, 667, 1285, 1380, 2100, 2334
BshNI GGYRCC 2 cut(s) 930, 1399
BsiHKAI GWGCWC 4 cut(s) 529, 697, 859, 1480
BsiHKCI CYCGRG 2 cut(s) 788, 1307
BsiSI CCGG 7 cut(s) 132, 179, 209, 222, 231, 428, 1219
BslFI GGGAC 4 cut(s) 248, 1373, 1677, 1899
BslI CCNNNNNNNGG 6 cut(s) 29, 487, 1141, 1142, 2361, 2568
BsmAI GTCTC 2 cut(s) 427, 2237
BsmFI GGGAC 4 cut(s) 248, 1373, 1677, 1899
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 7 cut(s) 155, 456, 667, 1285, 1380, 2100, 2334
Bso31I GGTCTC 2 cut(s) 427, 2237
BsoBI CYCGRG 2 cut(s) 788, 1307
Bsp119I TTCGAA 1 cut(s) 1657
Bsp1286I GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 1480
Bsp1720I GCTNAGC 1 cut(s) 597
Bsp19I CCATGG 2 cut(s) 898, 1256
BspANI GGCC 7 cut(s) 155, 456, 667, 1285, 1380, 2100, 2334
BspCNI CTCAG 6 cut(s) 178, 209, 679, 1129, 2011, 2245
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 6 cut(s) 207, 932, 1401, 2058, 2292, 2496
BspMI ACCTGC 5 cut(s) 95, 104, 781, 1258, 1688
BspPI GGATC 6 cut(s) 244, 779, 954, 1430, 1644, 2560
BspT104I TTCGAA 1 cut(s) 1657
BspT107I GGYRCC 2 cut(s) 930, 1399
BspTNI GGTCTC 2 cut(s) 427, 2237
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 3 cut(s) 1473, 2050, 2488
BsrFI RCCGGY 1 cut(s) 1218
BsrI ACTGG 7 cut(s) 625, 709, 1021, 1942, 2060, 2294, 2498
BssAI RCCGGY 1 cut(s) 1218
Bst2UI CCWGG 8 cut(s) 109, 254, 1202, 1538, 1727, 2122, 2356, 2563
Bst4CI ACNGT 3 cut(s) 891, 909, 1492
Bst6I CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 590
BstBI TTCGAA 1 cut(s) 1657
BstC8I GCNNGC 7 cut(s) 47, 114, 137, 693, 1314, 2098, 2332
BstDEI CTNAG 9 cut(s) 165, 196, 597, 687, 1137, 2019, 2139, 2253, 2580
BstDSI CCRYGG 2 cut(s) 898, 1256
BstF5I GGATG 3 cut(s) 388, 397, 2638
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 2 cut(s) 1061, 1123
BstHHI GCGC 5 cut(s) 7, 141, 905, 1060, 1122
BstMAI GTCTC 2 cut(s) 427, 2237
BstNI CCWGG 8 cut(s) 109, 254, 1202, 1538, 1727, 2122, 2356, 2563
BstNSI RCATGY 2 cut(s) 49, 617
BstPAI GACNNNNGTC 1 cut(s) 98
BstSLI GKGCMC 2 cut(s) 179, 859
BstSNI TACGTA 1 cut(s) 590
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 9 cut(s) 411, 433, 616, 706, 1508, 1871, 2509, 2512, 2600
BstX2I RGATCY 3 cut(s) 249, 1435, 1649
BstXI CCANNNNNNTGG 6 cut(s) 592, 875, 2000, 2108, 2342, 2438
BstYI RGATCY 3 cut(s) 249, 1435, 1649
BsuRI GGCC 7 cut(s) 155, 456, 667, 1285, 1380, 2100, 2334
BtgI CCRYGG 2 cut(s) 898, 1256
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 3 cut(s) 388, 397, 2638
BtsIMutI CAGTG 4 cut(s) 173, 1704, 1760, 2505
BveI ACCTGC 5 cut(s) 95, 104, 781, 1258, 1688
Cac8I GCNNGC 7 cut(s) 47, 114, 137, 693, 1314, 2098, 2332
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 5 cut(s) 7, 141, 905, 1060, 1122
Cfr10I RCCGGY 1 cut(s) 1218
Cfr13I GGNCC 3 cut(s) 764, 1018, 2559
CseI GACGC 1 cut(s) 88
Csp6I GTAC 5 cut(s) 931, 1493, 1673, 2051, 2285
CviQI GTAC 5 cut(s) 931, 1493, 1673, 2051, 2285
DdeI CTNAG 9 cut(s) 165, 196, 597, 687, 1137, 2019, 2139, 2253, 2580
DraI TTTAAA 2 cut(s) 1210, 2644
EaeI YGGCCR 4 cut(s) 454, 1378, 2098, 2332
Eam1104I CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
EarI CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
EciI GGCGGA 3 cut(s) 281, 344, 996
Eco105I TACGTA 1 cut(s) 590
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 427, 2237
Eco47I GGWCC 3 cut(s) 764, 1018, 2559
Eco47III AGCGCT 1 cut(s) 1121
Eco57I CTGAAG 2 cut(s) 491, 735
Eco88I CYCGRG 2 cut(s) 788, 1307
EcoRII CCWGG 8 cut(s) 107, 252, 1200, 1536, 1725, 2120, 2354, 2561
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 208
FaqI GGGAC 4 cut(s) 248, 1373, 1677, 1899
FauI CCCGC 2 cut(s) 194, 1603
FauNDI CATATG 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 844
FokI GGATG 3 cut(s) 395, 404, 2645
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 1287, 2534, 2616
GlaI GCGC 5 cut(s) 6, 140, 904, 1059, 1121
GsuI CTGGAG 3 cut(s) 2043, 2277, 2481
HaeII RGCGCY 2 cut(s) 1061, 1123
HaeIII GGCC 7 cut(s) 155, 456, 667, 1285, 1380, 2100, 2334
HapII CCGG 7 cut(s) 132, 179, 209, 222, 231, 428, 1219
HgaI GACGC 1 cut(s) 88
HhaI GCGC 5 cut(s) 7, 141, 905, 1060, 1122
Hin6I GCGC 5 cut(s) 5, 139, 903, 1058, 1120
HinP1I GCGC 5 cut(s) 5, 139, 903, 1058, 1120
HincII GTYRAC 2 cut(s) 554, 845
HindII GTYRAC 2 cut(s) 554, 845
HindIII AAGCTT 1 cut(s) 599
HpaII CCGG 7 cut(s) 132, 179, 209, 222, 231, 428, 1219
Hpy166II GTNNAC 6 cut(s) 554, 845, 857, 1732, 2197, 2609
Hpy188I TCNGA 6 cut(s) 70, 754, 1235, 2224, 2254, 2458
Hpy188III TCNNGA 9 cut(s) 389, 442, 481, 788, 924, 1507, 1823, 1993, 2431
Hpy8I GTNNAC 6 cut(s) 554, 845, 857, 1732, 2197, 2609
Hpy99I CGWCG 4 cut(s) 32, 38, 74, 163
HpyCH4III ACNGT 3 cut(s) 891, 909, 1492
HpyCH4IV ACGT 5 cut(s) 9, 375, 589, 2305, 2509
HpyF3I CTNAG 9 cut(s) 165, 196, 597, 687, 1137, 2019, 2139, 2253, 2580
HpySE526I ACGT 5 cut(s) 9, 375, 589, 2305, 2509
HspAI GCGC 5 cut(s) 5, 139, 903, 1058, 1120
KpnI GGTACC 1 cut(s) 934
Lsp1109I GCAGC 9 cut(s) 411, 433, 616, 706, 1508, 1871, 2509, 2512, 2600
MaeI CTAG 3 cut(s) 1287, 2534, 2616
MaeII ACGT 5 cut(s) 9, 375, 589, 2305, 2509
MbiI CCGCTC 1 cut(s) 321
MfeI CAATTG 1 cut(s) 1752
MflI RGATCY 3 cut(s) 249, 1435, 1649
MhlI GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 1480
MlsI TGGCCA 2 cut(s) 2100, 2334
MluNI TGGCCA 2 cut(s) 2100, 2334
MlyI GAGTC 5 cut(s) 236, 281, 1206, 1766, 1940
MmeI TCCRAC 7 cut(s) 93, 360, 1163, 1913, 2089, 2323, 2530
Mox20I TGGCCA 2 cut(s) 2100, 2334
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 2 cut(s) 2100, 2334
MseI TTAA 4 cut(s) 1209, 2373, 2445, 2643
MslI CAYNNNNRTG 4 cut(s) 510, 590, 2202, 2631
Msp20I TGGCCA 2 cut(s) 2100, 2334
MspA1I CMGCKG 4 cut(s) 424, 1383, 1793, 1862
MspI CCGG 7 cut(s) 132, 179, 209, 222, 231, 428, 1219
MunI CAATTG 1 cut(s) 1752
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 8 cut(s) 109, 254, 1202, 1538, 1727, 2122, 2356, 2563
MvnI CGCG 1 cut(s) 139
NciI CCSGG 3 cut(s) 132, 179, 232
NcoI CCATGG 2 cut(s) 898, 1256
NdeI CATATG 1 cut(s) 1129
NlaIV GGNNCC 6 cut(s) 207, 932, 1401, 2058, 2292, 2496
NmuCI GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 617
NspV TTCGAA 1 cut(s) 1657
PaeI GCATGC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 5 cut(s) 561, 1787, 1799, 2219, 2453
PleI GAGTC 5 cut(s) 235, 280, 1205, 1765, 1940
PpsI GAGTC 5 cut(s) 235, 280, 1205, 1765, 1940
Ppu21I YACGTR 1 cut(s) 590
PscI ACATGT 1 cut(s) 613
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 8 cut(s) 107, 252, 1200, 1536, 1725, 2120, 2354, 2561
PspGI CCWGG 8 cut(s) 107, 252, 1200, 1536, 1725, 2120, 2354, 2561
PspN4I GGNNCC 6 cut(s) 207, 932, 1401, 2058, 2292, 2496
PspPI GGNCC 3 cut(s) 764, 1018, 2559
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 249, 1435, 1649
PvuII CAGCTG 3 cut(s) 424, 1793, 1862
RsaI GTAC 5 cut(s) 932, 1494, 1674, 2052, 2286
RsaNI GTAC 5 cut(s) 931, 1493, 1673, 2051, 2285
RseI CAYNNNNRTG 4 cut(s) 510, 590, 2202, 2631
SalI GTCGAC 1 cut(s) 843
SaqAI TTAA 4 cut(s) 1209, 2373, 2445, 2643
Sau96I GGNCC 3 cut(s) 764, 1018, 2559
SchI GAGTC 5 cut(s) 236, 281, 1206, 1766, 1940
SduI GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 1480
SfuI TTCGAA 1 cut(s) 1657
SinI GGWCC 3 cut(s) 764, 1018, 2559
SmiMI CAYNNNNRTG 4 cut(s) 510, 590, 2202, 2631
SnaBI TACGTA 1 cut(s) 590
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 667
SspMI CTAG 3 cut(s) 1287, 2534, 2616
StuI AGGCCT 1 cut(s) 667
TaaI ACNGT 3 cut(s) 891, 909, 1492
TaiI ACGT 5 cut(s) 12, 378, 592, 2308, 2512
TaqI TCGA 5 cut(s) 158, 191, 844, 1447, 1657
TatI WGTACW 1 cut(s) 1672
TauI GCSGC 2 cut(s) 456, 1383
TfiI GAWTC 5 cut(s) 561, 1787, 1799, 2219, 2453
Tru1I TTAA 4 cut(s) 1209, 2373, 2445, 2643
Tru9I TTAA 4 cut(s) 1209, 2373, 2445, 2643
TscAI CASTG 4 cut(s) 173, 1711, 1767, 2505
TseFI GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
TseI GCWGC 9 cut(s) 421, 424, 629, 719, 1521, 1859, 2522, 2525, 2588
Tsp45I GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
TspDTI ATGAA 6 cut(s) 1760, 1812, 1845, 1992, 2196, 2430
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 4 cut(s) 173, 1711, 1767, 2505
VneI GTGCAC 1 cut(s) 855
VpaK11BI GGWCC 3 cut(s) 764, 1018, 2559
XapI RAATTY 2 cut(s) 1514, 1980
XceI RCATGY 2 cut(s) 49, 617
XcmI CCANNNNNNNNNTGG 2 cut(s) 488, 2254
XmiI GTMKAC 1 cut(s) 844
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 3 cut(s) 1287, 2534, 2616
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.