FvH4_5g24020

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000644
Physical Location & Seq
Reverse (-)
15280238 .. 15286032
5795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24020.t2

Sequence Viewer

Length: 2607 bp
ATGGCGCACGTTAGCCAGCTGCCGTGCGACGGCGAAGGAGTGTGCATGCTCTGCAAGGCGAAGCCGTCCGACGAAGAGAAGCTCTCCTGCAAGACCTGCATCACTCCATGGCACGCCGCCTGCCTGGCTCCCTCGTCCCGCCCCGAAACCCTAGCCTCCACGGCCCAGTGGGAGTGCCCCGACTGCAACTCCATCTCCGGCGACGCTCCGGCGGTCAAGTCCGGCGAGTCCGGGGACCTCATCGCGAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGGCAGGAGCTGATGAGCGGCAAGGCTGAGGTGGAGGAGGAGAAAGGAGGAGGGAAGAAGAAGGATGTGTTGGATGTGCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACGACACCATGCGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTTCAAAGATGGCAAGTCAACCTCGAATCAACTCTTCCCTTGTTGTTGCCATACGTATGGCTAAGCTTTCGAAATCACATGCTACTGGGGGTACTGCCAAGGTATATCATTACATACAAAACCAAAACAGGCCTGACAAAGCATACACTACTGAGCGGGCACAAAAGACTGGAAAAGCTAATGCTGCAAGCGGCAAGATATTTGTTACAATTCCTTCAGATCATTTTGGACCTATACCTGCTGAAAATGATCCCGAAAGAAATCAAGGTGTCATGGTTGGAGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACACTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGTGCTCAGTCTGTTGTGCTTTCTGGAGGGTACCTTGATGATGAAGATCATGGAGAGTGGTTCCTATACACAGGAAGTGGCGGAAGAGATCTGAGTGGCAACAAGCGAACTAATAAGGATCAGTCCTTTGACCAGACATTTGAAAAATATAACAAGGCACTCCAAGTAAGTTGCCAGAAGGGCTATCCTGTAAGGGTGGTAAGATCGCACAAGGAGAAGCGCTCTGCATATGCTCCTGAGAAAGGGTTGCGATATGATGGAGTTTATAGAATTGAAAAGTGTTGGAGAAAGGTGGGAGTCCAGGGCTTTAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAATGAACCTGCACCATGGACAAGTGATGAACATGGAGACCGGCCTAGACCATTGCCTGCTATACCTGAGTTGGCAAAAGCCACGAATATAACGGAGAGGAATGCAAGTCCATCATGGGACTTTGAGGAGAAAGATGGCTGCTGGAAATGGATAAAGGCACCACCCATGAGCAAAAAGCCTGTTCAAACATTAGATCCTGCAGATCGAAAGAGGTCAAGGACAGCAATAAGGAGAGCACAGAACATGACTGTAAGAGAGAGGCTCTTGAAAGAATTCAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCTGTTACCACCTCGTGCGGTCATAACTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGCTGGGAAGAGTGTGGTGACAGAGAGAAACAGAGGTGGAAGAAGCCTTCGAACTCAAAGAAATGTACTGACCTGCCCTTCCTGTCCCACCGATGTCACTGATTTTGTTCAAAATCCGCAGGTGAACACAGAGATTATGAAAGCAATTGAGTCACTAAAAGAAGAGGAAGAAAATGTGGATCCAGATAATGAAAGTGAAGAAATAGACATTCCTGACAATGAAAATAGCACAGAAATGCCTGATGAAGCAGCTGTGATAGATGGTGATCAGACTTCTCCTACAAAATGCAATCCCAAACGTCTGAAAGTTGATACTGGTAATGACTCGCCAAAGAGTGATATTGAGGAGGTAAATGAAATTTCTGATGTCCAGACTAATGGTGTCGATGGAGATACTGAGATGGAAATTGCCAAGGAAAGCAATGAGGTACAGGCTCCTGTTGAGAAACGTGGAAAAAAAACTGCTGGTCGAGCCGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGCTAAGCAAGCTGAGAGTGAAGAGAACGGCTCACCATCAAGTAATGAAGTTTCTGATCTCCACACTAATGGTGTCAATGGAGATTCTGATTTAGAGATGGCCAAGGAGACAACTTCTGAGATGGAAGTTGCCAAGGAAAGCAATGAGGAACAGGCTACAGTTGTAAAACGTGGGAAGAAAACTGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGAAAGAAAGAAGGCTACGGAAGCTATGAGTGAAGAGAATGGCTCACCATCAAGTAATGAAGTTTCTGATGGTGGCAATGGAGCTTCTGATTCTGGTATAGCCAAGGTAAGCATTGAGATACAGGCTCCAGTGGCAAAACGTGGGAAGAAAGTTGTGAAAGCTGCTGACGGTGTTGGATCAAGGACCAGAGGAAGGAAGAAGGCTGTGGCAGCAGAGATTGAAGAGAATGGTTCACCATCTAGTCCTCTCCATGTGGATGTGGATGTGGATTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

869

Amino Acids

94.92

Weight (kDa)

6.65

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 133 - 177 2.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 133 - 173 4.7e-06 Ring finger domain
zf-C3HC4 PF00097 134 - 172 2.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 134 - 172 3e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 264 - 413 4.6e-50 SAD/SRA domain
zf-RING_UBOX PF13445 506 - 530 1.5e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 506 - 532 3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1711
Acc36I ACCTGC 5 cut(s) 104, 775, 1252, 1682, 1711
Acc65I GGTACC 1 cut(s) 924
AccB1I GGYRCC 2 cut(s) 924, 1393
AccBSI CCGCTC 2 cut(s) 321, 685
AccI GTMKAC 1 cut(s) 838
AccII CGCG 1 cut(s) 245
AclWI GGATC 7 cut(s) 244, 773, 1020, 1424, 1775, 1788, 2512
AcoI YGGCCR 4 cut(s) 448, 2077, 2214, 2302
AcsI RAATTY 2 cut(s) 1508, 1959
AcuI CTGAAG 2 cut(s) 485, 729
AdeI CACNNNGTG 1 cut(s) 1560
AfaI GTAC 4 cut(s) 622, 926, 1668, 2031
AfeI AGCGCT 1 cut(s) 1115
AfiI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2106, 2520
AgsI TTSAA 9 cut(s) 397, 534, 1037, 1169, 1421, 1504, 1592, 1712, 2549
AjnI CCWGG 6 cut(s) 123, 252, 1194, 1530, 2099, 2324
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
Alw21I GWGCWC 4 cut(s) 523, 853, 901, 1474
Alw26I GTCTC 3 cut(s) 421, 1266, 2216
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 7 cut(s) 244, 773, 1020, 1424, 1775, 1788, 2512
AlwNI CAGNNNCTG 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 1115
AoxI GGCC 7 cut(s) 162, 448, 659, 1277, 2077, 2214, 2302
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 9 cut(s) 19, 415, 418, 713, 1374, 1515, 1850, 2489, 2537
ApoI RAATTY 2 cut(s) 1508, 1959
ArsI GACNNNNNNTTYG 2 cut(s) 1016, 1048
Asp700I GAANNNNTTC 2 cut(s) 470, 1508
Asp718I GGTACC 1 cut(s) 924
AspLEI GCGC 2 cut(s) 7, 1116
AspS9I GGNCC 4 cut(s) 163, 235, 758, 2511
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 8 cut(s) 1536, 1546, 1630, 1735, 1877, 2139, 2364, 2553
AsuII TTCGAA 2 cut(s) 599, 1651
AvaII GGWCC 3 cut(s) 235, 758, 2511
BaeGI GKGCMC 3 cut(s) 179, 691, 853
BalI TGGCCA 2 cut(s) 2216, 2304
BamHI GGATCC 1 cut(s) 1780
BanI GGYRCC 2 cut(s) 924, 1393
BauI CACGAG 1 cut(s) 1558
Bbv12I GWGCWC 4 cut(s) 523, 853, 901, 1474
BbvCI CCTCAGC 1 cut(s) 330
BbvI GCAGC 9 cut(s) 6, 405, 427, 700, 1361, 1502, 1862, 2476, 2549
BceAI ACGGC 6 cut(s) 7, 46, 49, 177, 501, 2158
BciT130I CCWGG 6 cut(s) 125, 254, 1196, 1532, 2101, 2326
BclI TGATCA 1 cut(s) 1867
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 3 cut(s) 421, 1266, 2216
BfaI CTAG 3 cut(s) 152, 1281, 2568
BfmI CTRYAG 2 cut(s) 1434, 2271
BfoI RGCGCY 1 cut(s) 1117
BfuAI ACCTGC 5 cut(s) 104, 775, 1252, 1682, 1711
BglI GCCNNNNNGGC 3 cut(s) 125, 161, 1074
BglII AGATCT 1 cut(s) 982
BlpI GCTNAGC 2 cut(s) 591, 2118
Bme1390I CCNGG 7 cut(s) 125, 232, 254, 1196, 1532, 2101, 2326
Bme18I GGWCC 3 cut(s) 235, 758, 2511
BmgT120I GGNCC 4 cut(s) 163, 235, 758, 2511
BmiI GGNNCC 8 cut(s) 129, 236, 926, 956, 1395, 1782, 2037, 2454
BmrFI CCNGG 7 cut(s) 125, 232, 254, 1196, 1532, 2101, 2326
BmrI ACTGGG 2 cut(s) 160, 624
BmsI GCATC 1 cut(s) 108
BmuI ACTGGG 2 cut(s) 160, 624
BpmI CTGGAG 2 cut(s) 939, 2439
Bpu10I CCTNAGC 1 cut(s) 330
Bpu1102I GCTNAGC 2 cut(s) 591, 2118
Bpu14I TTCGAA 2 cut(s) 599, 1651
BpuMI CCSGG 1 cut(s) 232
BsaAI YACGTR 1 cut(s) 584
BsaBI GATNNNNATC 2 cut(s) 939, 1866
BsaI GGTCTC 2 cut(s) 421, 1266
BsaXI ACNNNNNCTCC 4 cut(s) 173, 179, 203, 209
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2106, 2520
Bse118I RCCGGY 3 cut(s) 1212, 1275, 2075
Bse1I ACTGG 5 cut(s) 166, 619, 703, 1921, 2456
Bse3DI GCAATG 4 cut(s) 1286, 2029, 2263, 2410
Bse8I GATNNNNATC 2 cut(s) 939, 1866
BseBI CCWGG 6 cut(s) 125, 254, 1196, 1532, 2101, 2326
BseGI GGATG 5 cut(s) 373, 382, 391, 2590, 2596
BseJI GATNNNNATC 2 cut(s) 939, 1866
BseLI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2106, 2520
BseMI GCAATG 4 cut(s) 1286, 2029, 2263, 2410
BseMII CTCAG 9 cut(s) 321, 672, 914, 977, 1122, 1293, 1989, 2118, 2223
BseNI ACTGG 5 cut(s) 166, 619, 703, 1921, 2456
BseRI GAGGAG 7 cut(s) 299, 302, 353, 356, 366, 1376, 1961
BseSI GKGCMC 3 cut(s) 179, 691, 853
BseXI GCAGC 9 cut(s) 6, 405, 427, 700, 1361, 1502, 1862, 2476, 2549
BseYI CCCAGC 1 cut(s) 1604
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 7 cut(s) 164, 450, 661, 1279, 2079, 2216, 2304
BshNI GGYRCC 2 cut(s) 924, 1393
BsiHKAI GWGCWC 4 cut(s) 523, 853, 901, 1474
BsiSI CCGG 8 cut(s) 198, 209, 222, 231, 422, 1213, 1276, 2076
BslFI GGGAC 4 cut(s) 121, 248, 1367, 1671
BslI CCNNNNNNNGG 5 cut(s) 29, 481, 1136, 2106, 2520
BsmAI GTCTC 3 cut(s) 421, 1266, 2216
BsmFI GGGAC 4 cut(s) 121, 248, 1367, 1671
BsmI GAATGC 1 cut(s) 1342
BsnI GGCC 7 cut(s) 164, 450, 661, 1279, 2079, 2216, 2304
Bso31I GGTCTC 2 cut(s) 421, 1266
Bsp119I TTCGAA 2 cut(s) 599, 1651
Bsp1286I GDGCHC 6 cut(s) 179, 523, 691, 853, 901, 1474
Bsp1720I GCTNAGC 2 cut(s) 591, 2118
Bsp19I CCATGG 3 cut(s) 107, 892, 1250
Bsp68I TCGCGA 1 cut(s) 245
BspANI GGCC 7 cut(s) 164, 450, 661, 1279, 2079, 2216, 2304
BspCNI CTCAG 9 cut(s) 322, 673, 913, 978, 1123, 1294, 1990, 2119, 2224
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 8 cut(s) 129, 236, 926, 956, 1395, 1782, 2037, 2454
BspMAI CTGCAG 1 cut(s) 1438
BspMI ACCTGC 5 cut(s) 104, 775, 1252, 1682, 1711
BspPI GGATC 7 cut(s) 244, 773, 1020, 1424, 1775, 1788, 2512
BspT104I TTCGAA 2 cut(s) 599, 1651
BspT107I GGYRCC 2 cut(s) 924, 1393
BspTNI GGTCTC 2 cut(s) 421, 1266
BsrBI CCGCTC 2 cut(s) 321, 685
BsrDI GCAATG 4 cut(s) 1286, 2029, 2263, 2410
BsrFI RCCGGY 3 cut(s) 1212, 1275, 2075
BsrI ACTGG 5 cut(s) 166, 619, 703, 1921, 2456
BssAI RCCGGY 3 cut(s) 1212, 1275, 2075
BssSI CACGAG 1 cut(s) 1558
Bst2BI CACGAG 1 cut(s) 1558
Bst2UI CCWGG 6 cut(s) 125, 254, 1196, 1532, 2101, 2326
Bst4CI ACNGT 4 cut(s) 885, 1486, 2275, 2498
Bst6I CTCTTC 8 cut(s) 69, 568, 973, 1604, 1758, 2130, 2355, 2544
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 584
BstBI TTCGAA 2 cut(s) 599, 1651
BstDSI CCRYGG 4 cut(s) 107, 159, 892, 1250
BstENI CCTNNNNNAGG 1 cut(s) 1134
BstF5I GGATG 5 cut(s) 373, 382, 391, 2590, 2596
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 1117
BstHHI GCGC 2 cut(s) 7, 1116
BstMAI GTCTC 3 cut(s) 421, 1266, 2216
BstNI CCWGG 6 cut(s) 125, 254, 1196, 1532, 2101, 2326
BstNSI RCATGY 2 cut(s) 49, 611
BstSCI CCNGG 7 cut(s) 123, 230, 252, 1194, 1530, 2099, 2324
BstSFI CTRYAG 2 cut(s) 1434, 2271
BstSLI GKGCMC 3 cut(s) 179, 691, 853
BstSNI TACGTA 1 cut(s) 584
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 9 cut(s) 6, 405, 427, 700, 1361, 1502, 1862, 2476, 2549
BstX2I RGATCY 4 cut(s) 249, 982, 1429, 1780
BstXI CCANNNNNNTGG 6 cut(s) 586, 869, 1979, 2087, 2183, 2312
BstYI RGATCY 4 cut(s) 249, 982, 1429, 1780
BsuRI GGCC 7 cut(s) 164, 450, 661, 1279, 2079, 2216, 2304
BtgI CCRYGG 4 cut(s) 107, 159, 892, 1250
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 5 cut(s) 373, 382, 391, 2590, 2596
BtsIMutI CAGTG 3 cut(s) 173, 1698, 2463
BtuMI TCGCGA 1 cut(s) 245
BveI ACCTGC 5 cut(s) 104, 775, 1252, 1682, 1711
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 2 cut(s) 7, 1116
Cfr10I RCCGGY 3 cut(s) 1212, 1275, 2075
Cfr13I GGNCC 4 cut(s) 163, 235, 758, 2511
CseI GACGC 1 cut(s) 212
Csp6I GTAC 4 cut(s) 621, 925, 1667, 2030
CviQI GTAC 4 cut(s) 621, 925, 1667, 2030
DraIII CACNNNGTG 1 cut(s) 1560
EaeI YGGCCR 4 cut(s) 448, 2077, 2214, 2302
Eam1104I CTCTTC 8 cut(s) 69, 568, 973, 1604, 1758, 2130, 2355, 2544
EarI CTCTTC 8 cut(s) 69, 568, 973, 1604, 1758, 2130, 2355, 2544
EciI GGCGGA 2 cut(s) 281, 990
Eco105I TACGTA 1 cut(s) 584
Eco147I AGGCCT 1 cut(s) 661
Eco31I GGTCTC 2 cut(s) 421, 1266
Eco47I GGWCC 3 cut(s) 235, 758, 2511
Eco47III AGCGCT 1 cut(s) 1115
Eco57I CTGAAG 2 cut(s) 485, 729
EcoNI CCTNNNNNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 1508
EcoRII CCWGG 6 cut(s) 123, 252, 1194, 1530, 2099, 2324
FaqI GGGAC 4 cut(s) 121, 248, 1367, 1671
FauI CCCGC 2 cut(s) 146, 678
FauNDI CATATG 1 cut(s) 1123
FbaI TGATCA 1 cut(s) 1867
FblI GTMKAC 1 cut(s) 838
FokI GGATG 5 cut(s) 380, 389, 398, 2597, 2603
FspBI CTAG 3 cut(s) 152, 1281, 2568
GlaI GCGC 2 cut(s) 6, 1115
GsaI CCCAGC 1 cut(s) 1608
GsuI CTGGAG 2 cut(s) 939, 2439
HaeII RGCGCY 1 cut(s) 1117
HaeIII GGCC 7 cut(s) 164, 450, 661, 1279, 2079, 2216, 2304
HapII CCGG 8 cut(s) 198, 209, 222, 231, 422, 1213, 1276, 2076
HgaI GACGC 1 cut(s) 212
HhaI GCGC 2 cut(s) 7, 1116
Hin6I GCGC 2 cut(s) 5, 1114
HinP1I GCGC 2 cut(s) 5, 1114
HincII GTYRAC 2 cut(s) 548, 839
HindII GTYRAC 2 cut(s) 548, 839
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 8 cut(s) 227, 272, 555, 1191, 1751, 1925, 2198, 2417
HpaII CCGG 8 cut(s) 198, 209, 222, 231, 422, 1213, 1276, 2076
HphI GGTGA 8 cut(s) 1536, 1546, 1630, 1735, 1877, 2139, 2364, 2553
Hpy166II GTNNAC 5 cut(s) 548, 839, 851, 1726, 2561
Hpy8I GTNNAC 5 cut(s) 548, 839, 851, 1726, 2561
Hpy99I CGWCG 3 cut(s) 32, 74, 206
HpyCH4III ACNGT 4 cut(s) 885, 1486, 2275, 2498
HpyCH4IV ACGT 6 cut(s) 9, 583, 1900, 2050, 2284, 2467
HpySE526I ACGT 6 cut(s) 9, 583, 1900, 2050, 2284, 2467
HspAI GCGC 2 cut(s) 5, 1114
KpnI GGTACC 1 cut(s) 928
KroI GCCGGC 1 cut(s) 2075
KroNI GCCGGC 1 cut(s) 2077
Ksp22I TGATCA 1 cut(s) 1867
Lsp1109I GCAGC 9 cut(s) 6, 405, 427, 700, 1361, 1502, 1862, 2476, 2549
LweI GCATC 1 cut(s) 108
MaeI CTAG 3 cut(s) 152, 1281, 2568
MaeII ACGT 6 cut(s) 9, 583, 1900, 2050, 2284, 2467
MaeIII GTNAC 7 cut(s) 433, 733, 1232, 1549, 1618, 1696, 1752
MbiI CCGCTC 2 cut(s) 321, 685
MfeI CAATTG 1 cut(s) 1746
MflI RGATCY 4 cut(s) 249, 982, 1429, 1780
MhlI GDGCHC 6 cut(s) 179, 523, 691, 853, 901, 1474
MlsI TGGCCA 2 cut(s) 2216, 2304
MluCI AATT 8 cut(s) 454, 525, 738, 1164, 1508, 1746, 1959, 2007
MluNI TGGCCA 2 cut(s) 2216, 2304
MlyI GAGTC 5 cut(s) 236, 281, 1200, 1760, 1919
MmeI TCCRAC 7 cut(s) 93, 354, 787, 1157, 2068, 2293, 2482
Mox20I TGGCCA 2 cut(s) 2216, 2304
MroNI GCCGGC 1 cut(s) 2075
MroXI GAANNNNTTC 2 cut(s) 470, 1508
MscI TGGCCA 2 cut(s) 2216, 2304
MseI TTAA 1 cut(s) 1203
MslI CAYNNNNRTG 5 cut(s) 504, 584, 1835, 2181, 2583
Msp20I TGGCCA 2 cut(s) 2216, 2304
MspA1I CMGCKG 4 cut(s) 19, 418, 1515, 1853
MspI CCGG 8 cut(s) 198, 209, 222, 231, 422, 1213, 1276, 2076
MspR9I CCNGG 7 cut(s) 125, 232, 254, 1196, 1532, 2101, 2326
MunI CAATTG 1 cut(s) 1746
Mva1269I GAATGC 1 cut(s) 1342
MvaI CCWGG 6 cut(s) 125, 254, 1196, 1532, 2101, 2326
MvnI CGCG 1 cut(s) 245
NaeI GCCGGC 1 cut(s) 2077
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 3 cut(s) 107, 892, 1250
NdeI CATATG 1 cut(s) 1123
NgoMIV GCCGGC 1 cut(s) 2075
NlaIV GGNNCC 8 cut(s) 129, 236, 926, 956, 1395, 1782, 2037, 2454
NmuCI GTSAC 5 cut(s) 433, 1232, 1618, 1696, 1752
NruI TCGCGA 1 cut(s) 245
NspI RCATGY 2 cut(s) 49, 611
NspV TTCGAA 2 cut(s) 599, 1651
PaeI GCATGC 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 1711
PceI AGGCCT 1 cut(s) 661
PctI GAATGC 1 cut(s) 1342
PdiI GCCGGC 1 cut(s) 2077
PdmI GAANNNNTTC 2 cut(s) 470, 1508
PfeI GAWTC 3 cut(s) 555, 2198, 2417
PleI GAGTC 5 cut(s) 235, 280, 1199, 1759, 1919
PpsI GAGTC 5 cut(s) 235, 280, 1199, 1759, 1919
Ppu21I YACGTR 1 cut(s) 584
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 6 cut(s) 123, 252, 1194, 1530, 2099, 2324
PspFI CCCAGC 1 cut(s) 1604
PspGI CCWGG 6 cut(s) 123, 252, 1194, 1530, 2099, 2324
PspN4I GGNNCC 8 cut(s) 129, 236, 926, 956, 1395, 1782, 2037, 2454
PspPI GGNCC 4 cut(s) 163, 235, 758, 2511
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 1 cut(s) 1438
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 4 cut(s) 249, 982, 1429, 1780
PvuII CAGCTG 4 cut(s) 19, 418, 1515, 1853
RruI TCGCGA 1 cut(s) 245
RsaI GTAC 4 cut(s) 622, 926, 1668, 2031
RsaNI GTAC 4 cut(s) 621, 925, 1667, 2030
RseI CAYNNNNRTG 5 cut(s) 504, 584, 1835, 2181, 2583
SalI GTCGAC 1 cut(s) 837
SaqAI TTAA 1 cut(s) 1203
Sau96I GGNCC 4 cut(s) 163, 235, 758, 2511
SchI GAGTC 5 cut(s) 236, 281, 1200, 1760, 1919
ScrFI CCNGG 7 cut(s) 125, 232, 254, 1196, 1532, 2101, 2326
SduI GDGCHC 6 cut(s) 179, 523, 691, 853, 901, 1474
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 2 cut(s) 1434, 2271
SfuI TTCGAA 2 cut(s) 599, 1651
SinI GGWCC 3 cut(s) 235, 758, 2511
SmiMI CAYNNNNRTG 5 cut(s) 504, 584, 1835, 2181, 2583
SnaBI TACGTA 1 cut(s) 584
SphI GCATGC 1 cut(s) 49
Sse9I AATT 8 cut(s) 454, 525, 738, 1164, 1508, 1746, 1959, 2007
SseBI AGGCCT 1 cut(s) 661
SspMI CTAG 3 cut(s) 152, 1281, 2568
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 7 cut(s) 123, 230, 252, 1194, 1530, 2099, 2324
TaaI ACNGT 4 cut(s) 885, 1486, 2275, 2498
TaiI ACGT 6 cut(s) 12, 586, 1903, 2053, 2287, 2470
TaqI TCGA 7 cut(s) 553, 599, 838, 1441, 1651, 1986, 2071
TasI AATT 8 cut(s) 454, 525, 738, 1164, 1508, 1746, 1959, 2007
TatI WGTACW 1 cut(s) 1666
TauI GCSGC 5 cut(s) 119, 307, 324, 450, 723
TfiI GAWTC 3 cut(s) 555, 2198, 2417
Tru1I TTAA 1 cut(s) 1203
Tru9I TTAA 1 cut(s) 1203
TscAI CASTG 3 cut(s) 173, 1705, 2463
TseFI GTSAC 5 cut(s) 433, 1232, 1618, 1696, 1752
TseI GCWGC 9 cut(s) 19, 415, 418, 713, 1374, 1515, 1850, 2489, 2537
Tsp45I GTSAC 5 cut(s) 433, 1232, 1618, 1696, 1752
TspGWI ACGGA 3 cut(s) 296, 1343, 2360
TspRI CASTG 3 cut(s) 173, 1705, 2463
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 3 cut(s) 235, 758, 2511
XagI CCTNNNNNAGG 1 cut(s) 1134
XapI RAATTY 2 cut(s) 1508, 1959
XceI RCATGY 2 cut(s) 49, 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 2 cut(s) 470, 1508
XspI CTAG 3 cut(s) 152, 1281, 2568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.