Rh7BG098500

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000644
Physical Location & Seq
Forward (+)
7263564 .. 7268820
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG098500.1

Sequence Viewer

Length: 2220 bp
ATGGCGCACGTTAGCCAGCTTCCGTGCGACGGAGACGGCGTCTGCATGCTCTGCAAAACGACGCCGTCGCACGAGGACCAGCTCATCTGCAGGACCTGCGTCACGCCGTGGCACGTCAGCTGCCTCTCGTCCCGTCCCGAATCGCTGGCTTCCACTCTCCAGTGGGAGTGCCCGGACTGTTCCTCGCTCTCCGGCGACACGCCGGCCGTGAAATCGGCCGTCTCCGGCGAGCTGATCGCCGCGATCCGGGCGATCGAGGCCGATGCGGCGCTGACGGAGCAGCAGAAGGCGAGTAAGCGACAGGAGCTGATGAGCGGGAAGGCTCTGCCGGAGGAAGAAGAAGGCGCCGGCAGGAAGGGAGTAAACGACGGCGTTTTGGATGGGATTTTCAATTGCTCGTTCTGTATACAACTACCGGAGAGACCTGTGACGACACCATGTGGCCACAATTTCTGCTTGAAGTGCTTCCAGAAATGGGTTGGACAAGGGAAGCGTACTTGTGCAAAGTGCCGCCATGCCATTCCTTCCAAAATGGCAAGTCAACCCCGCATCAATTCTTCACTTGTAGTTGCCATACGTATGGCTAAGATGTCAAAATCATATGTTACTGAGGGGCCTGCTAAGGTATACCACTTTGTCCGAAACCAAGACAGACCTGACAAAGCTTTCACTACCGAGCGGGCGCAGAAAACTGGGAAATCAAATGCTGCAAGTGGCAAGATATTTGTCACAATTCCAGCTGATCATTTTGGACCTATACCTGCTGAATATGATCCTGAGAGAAACCAAGGTGTGCTGGTAGGGGAATGCTGGGAGGATAGGCTTGAATGTCGACAGTGGGGTGCACACTTTCCCCATGTTGCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCAGTTGTGCTTTCAGGAGGGTACCTTGATGATGAGGATCATGGAGAGTGGTTCTTATACACAGGAAGTGGTGGAAGGGATTTGAGTGGGAATAAGCGAACAAACAAGGATCAGTCATTTGACCAGACATTTGAGAAATATAACAAGGCTCTCCAAGTTAGTTGCCTGAAAGGGTATCCTGTACGGGTGGTAAGATCGCACAAGGAGAAGCGCTCCTCATATGCCCCTGAGAAAGGGTTGCGGTATGATGGAGTCTATAGAATTGAAAAATGTTGGACAAAGGTGGGAATCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTTAGATGTGACAATGAACCTGCACCATGGACGAGTGATGAACATGGGGACCGGCCTAGACCTTTACCTCCAATACCTGAGCTGAAGAAAGCTACAAGTATAATAGAGAGGAAGTCAAGTCCATCGTGGGACTTTGAGGAGAAAGATGGCTGCTGGAAGTGGAAAAAGTCACCACCTATCAGTAAAAAAACCGTTCAGACATTAGATTCTGCTGATCGAAAGAGGTCAAGAAAAGGAAAGAGGAAGGCAAAAAATATTACTGTGCGAGAGAGGCTCTTAAGAGAATTCAGCTGCCTTGTTTGCGGGGAGGTCATGACATTACCAATGACCACACCTTGTGCTCATAACTTTTGCAAATCCTGTTTGGAAGGCGCTTTTGCGGGAAAGAGCTTGGTGAGAGAAAGAAACAGAGGGGGAAGAACCCTTCGAACCCAAAAGAATATAATGAGCTGCCCTTCTTGTCCTACTGATATCTCTGATTTTCTTCAAGATCCTCAGGTGAATAGAGAGTTAATGGATGCGATTGAGTCACTTAAGTCACAGAATGAAGAGGAAGAGAATGAGGATTCAGCTGAAGAAATGAGTGAAGATGTGGATAATTCTGACAACGAAATTAGCACTGAAATGGTTGAAGAAGCAGGTGTTAAAGAATGTGCTGAGGGTTCTCCAACAAACTGCAATCCCGAAAGGAGTCCCAAACGACAGAAAGTTGATGCTGGTGAAGGCTCACCAAAAACCAATGATGAGGAGGTAAATTGCATTCCTGTCGCCCAGACTAATGGCAAAGGCTCACCGGAAACCGATACTGAAGAGGTAAATGAAACTCCTGTCGCCCATACTAATGGCAAGGGCTCACCGGCTCCAGTTGCAAAATGTGGCAAGCAAGATGCTGGTCCGGTTGGCGTTGCAGCAAATACTAGGAGGAGGAAGAAGGCTGAAGTAGTGGGTAAAGATAATGGTTCCCCATCAAGTCCTCTGCTTGTGCAATCTGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

739

Amino Acids

81.44

Weight (kDa)

7.39

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 131 - 171 4.4e-06 Ring finger domain
zf-C3HC4_3 PF13920 131 - 174 5.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 132 - 170 4.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 132 - 170 1e-05 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 262 - 411 1.4e-49 SAD/SRA domain
zf-RING_UBOX PF13445 504 - 535 4.8e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1847
Acc36I ACCTGC 4 cut(s) 104, 769, 1246, 1847
Acc65I GGTACC 1 cut(s) 918
AccB1I GGYRCC 2 cut(s) 344, 918
AccBSI CCGCTC 2 cut(s) 315, 679
AccI GTMKAC 3 cut(s) 406, 627, 832
AccII CGCG 1 cut(s) 242
AciI CCGC 9 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521, 1598
AclWI GGATC 5 cut(s) 238, 767, 942, 1014, 1703
AcoI YGGCCR 3 cut(s) 204, 216, 442
AcsI RAATTY 1 cut(s) 1502
AcuI CTGAAG 4 cut(s) 1322, 1812, 2046, 2175
AcyI GRCGYC 3 cut(s) 39, 62, 345
AdeI CACNNNGTG 3 cut(s) 108, 440, 1556
AfaI GTAC 3 cut(s) 496, 920, 1080
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
AflII CTTAAG 2 cut(s) 1495, 1751
AgsI TTSAA 7 cut(s) 391, 460, 827, 1163, 1706, 1850, 2216
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 1188
Alw21I GWGCWC 3 cut(s) 847, 895, 1561
Alw26I GTCTC 3 cut(s) 27, 226, 415
Alw44I GTGCAC 2 cut(s) 843, 891
AlwI GGATC 5 cut(s) 238, 767, 942, 1014, 1703
AlwNI CAGNNNCTG 2 cut(s) 96, 307
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 6 cut(s) 204, 216, 258, 442, 614, 1271
ApaLI GTGCAC 2 cut(s) 843, 891
ApeKI GCWGC 7 cut(s) 120, 280, 707, 1368, 1509, 1668, 2126
ApoI RAATTY 1 cut(s) 1502
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
Asp700I GAANNNNTTC 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 918
AspLEI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
AspS9I GGNCC 6 cut(s) 76, 93, 614, 752, 1267, 2111
AsuC2I CCSGG 2 cut(s) 173, 248
AsuHPI GGTGA 7 cut(s) 1380, 1624, 1729, 1938, 1949, 2001, 2064
AsuII TTCGAA 1 cut(s) 1645
AvaII GGWCC 5 cut(s) 76, 93, 752, 1267, 2111
AxyI CCTNAGG 1 cut(s) 1713
BaeGI GKGCMC 3 cut(s) 173, 847, 895
BalI TGGCCA 1 cut(s) 444
BanI GGYRCC 2 cut(s) 344, 918
BanII GRGCYC 1 cut(s) 2072
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 847, 895, 1561
BbvCI CCTCAGC 1 cut(s) 1875
BbvI GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BccI CCATC 5 cut(s) 374, 1139, 1348, 1358, 2191
BceAI ACGGC 6 cut(s) 49, 52, 91, 191, 203, 385
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1966, 2000
BciT130I CCWGG 1 cut(s) 1190
BciVI GTATCC 1 cut(s) 1083
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 2 cut(s) 173, 248
BcoDI GTCTC 3 cut(s) 27, 226, 415
BfaI CTAG 2 cut(s) 1275, 2136
BfmI CTRYAG 2 cut(s) 88, 1153
BfoI RGCGCY 4 cut(s) 272, 348, 1111, 1593
BfrI CTTAAG 2 cut(s) 1495, 1751
BfuAI ACCTGC 4 cut(s) 104, 769, 1246, 1847
BfuI GTATCC 1 cut(s) 1083
BglI GCCNNNNNGGC 1 cut(s) 266
Bme1390I CCNGG 3 cut(s) 173, 248, 1190
Bme18I GGWCC 5 cut(s) 76, 93, 752, 1267, 2111
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 6 cut(s) 76, 93, 614, 752, 1267, 2111
BmiI GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2179
BmrFI CCNGG 3 cut(s) 173, 248, 1190
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
BmuI ACTGGG 1 cut(s) 702
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 1476, 1508
BpmI CTGGAG 2 cut(s) 143, 2064
Bpu10I CCTNAGC 3 cut(s) 621, 1296, 1875
Bpu14I TTCGAA 1 cut(s) 1645
BpuMI CCSGG 2 cut(s) 173, 248
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 3 cut(s) 39, 62, 345
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BsaWI WCCGGW 3 cut(s) 415, 2011, 2113
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
Bse118I RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
Bse1I ACTGG 3 cut(s) 160, 697, 2081
Bse21I CCTNAGG 1 cut(s) 1713
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 1 cut(s) 1190
BseDI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BseGI GGATG 2 cut(s) 385, 1741
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BseMII CTCAG 6 cut(s) 600, 768, 1116, 1287, 1727, 1866
BseNI ACTGG 3 cut(s) 160, 697, 2081
BseRI GAGGAG 4 cut(s) 1102, 1370, 1979, 2155
BseSI GKGCMC 3 cut(s) 173, 847, 895
BseX3I CGGCCG 2 cut(s) 204, 216
BseXI GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BseYI CCCAGC 1 cut(s) 810
BsgI GTGCAG 1 cut(s) 1224
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 3 cut(s) 207, 219, 255
BshFI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BshNI GGYRCC 2 cut(s) 344, 918
BsiEI CGRYCG 3 cut(s) 207, 219, 255
BsiHKAI GWGCWC 3 cut(s) 847, 895, 1561
BslFI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
BslI CCNNNNNNNGG 4 cut(s) 29, 246, 475, 1130
BsmAI GTCTC 3 cut(s) 27, 226, 415
BsmBI CGTCTC 2 cut(s) 27, 226
BsmFI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
BsmI GAATGC 2 cut(s) 812, 1977
BsnI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
Bso31I GGTCTC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 1645
Bsp1286I GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
Bsp19I CCATGG 2 cut(s) 886, 1244
BspACI CCGC 9 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521, 1598
BspANI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BspCNI CTCAG 6 cut(s) 601, 769, 1117, 1288, 1726, 1867
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 1530
BspLI GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2179
BspMAI CTGCAG 1 cut(s) 92
BspMI ACCTGC 4 cut(s) 104, 769, 1246, 1847
BspPI GGATC 5 cut(s) 238, 767, 942, 1014, 1703
BspT104I TTCGAA 1 cut(s) 1645
BspT107I GGYRCC 2 cut(s) 344, 918
BspTI CTTAAG 2 cut(s) 1495, 1751
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 315, 679
BsrFI RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
BsrI ACTGG 3 cut(s) 160, 697, 2081
BssAI RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
BssECI CCNNGG 5 cut(s) 107, 787, 886, 1189, 1244
BssNAI GTATAC 2 cut(s) 407, 628
BssNI GRCGYC 3 cut(s) 39, 62, 345
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 787, 886, 1244
Bst1107I GTATAC 2 cut(s) 407, 628
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1190
Bst4CI ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
Bst6I CTCTTC 3 cut(s) 1761, 1767, 2022
BstACI GRCGYC 3 cut(s) 39, 62, 345
BstAFI CTTAAG 2 cut(s) 1495, 1751
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstBAI YACGTR 1 cut(s) 578
BstBI TTCGAA 1 cut(s) 1645
BstC8I GCNNGC 9 cut(s) 17, 47, 147, 204, 230, 349, 618, 681, 2099
BstDEI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
BstDSI CCRYGG 3 cut(s) 107, 886, 1244
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 2 cut(s) 385, 1741
BstFNI CGCG 1 cut(s) 242
BstH2I RGCGCY 4 cut(s) 272, 348, 1111, 1593
BstHHI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
BstMAI GTCTC 3 cut(s) 27, 226, 415
BstMCI CGRYCG 3 cut(s) 207, 219, 255
BstNI CCWGG 1 cut(s) 1190
BstNSI RCATGY 1 cut(s) 49
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 171, 246, 1188
BstSFI CTRYAG 2 cut(s) 88, 1153
BstSLI GKGCMC 3 cut(s) 173, 847, 895
BstSNI TACGTA 1 cut(s) 578
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
BstX2I RGATCY 1 cut(s) 1708
BstXI CCANNNNNNTGG 4 cut(s) 580, 863, 1997, 2060
BstYI RGATCY 1 cut(s) 1708
BstZ17I GTATAC 2 cut(s) 407, 628
BstZI CGGCCG 2 cut(s) 204, 216
Bsu36I CCTNAGG 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
BtgI CCRYGG 3 cut(s) 107, 886, 1244
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 385, 1741
BtsIMutI CAGTG 3 cut(s) 167, 842, 1836
BveI ACCTGC 4 cut(s) 104, 769, 1246, 1847
Cac8I GCNNGC 9 cut(s) 17, 47, 147, 204, 230, 349, 618, 681, 2099
CaiI CAGNNNCTG 2 cut(s) 96, 307
CciI TCATGA 1 cut(s) 1530
CfoI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
Cfr10I RCCGGY 5 cut(s) 202, 347, 1206, 1269, 2074
Cfr13I GGNCC 6 cut(s) 76, 93, 614, 752, 1267, 2111
CseI GACGC 3 cut(s) 28, 70, 88
Csp6I GTAC 3 cut(s) 495, 919, 1079
CviAII CATG 9 cut(s) 46, 438, 515, 857, 887, 938, 1245, 1262, 1531
CviQI GTAC 3 cut(s) 495, 919, 1079
DdeI CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
DinI GGCGCC 1 cut(s) 346
DraIII CACNNNGTG 3 cut(s) 108, 440, 1556
EaeI YGGCCR 3 cut(s) 204, 216, 442
EagI CGGCCG 2 cut(s) 204, 216
Eam1104I CTCTTC 3 cut(s) 1761, 1767, 2022
EarI CTCTTC 3 cut(s) 1761, 1767, 2022
EclXI CGGCCG 2 cut(s) 204, 216
Eco105I TACGTA 1 cut(s) 578
Eco130I CCWWGG 3 cut(s) 787, 886, 1244
Eco24I GRGCYC 1 cut(s) 2072
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 1690
Eco47I GGWCC 5 cut(s) 76, 93, 752, 1267, 2111
Eco47III AGCGCT 1 cut(s) 1109
Eco52I CGGCCG 2 cut(s) 204, 216
Eco57I CTGAAG 4 cut(s) 1322, 1812, 2046, 2175
Eco81I CCTNAGG 1 cut(s) 1713
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 93, 614
EcoRI GAATTC 1 cut(s) 1502
EcoRII CCWGG 1 cut(s) 1188
EcoRV GATATC 1 cut(s) 1690
EcoT14I CCWWGG 3 cut(s) 787, 886, 1244
EcoT38I GRGCYC 1 cut(s) 2072
EgeI GGCGCC 1 cut(s) 346
EheI GGCGCC 1 cut(s) 346
ErhI CCWWGG 3 cut(s) 787, 886, 1244
Esp3I CGTCTC 2 cut(s) 27, 226
FaeI CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
FaqI GGGAC 5 cut(s) 115, 120, 1280, 1361, 1896
FatI CATG 9 cut(s) 45, 437, 514, 856, 886, 937, 1244, 1261, 1530
FauI CCCGC 5 cut(s) 308, 554, 672, 1514, 1591
FauNDI CATATG 2 cut(s) 601, 1117
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 3 cut(s) 406, 627, 832
FokI GGATG 2 cut(s) 392, 1748
FriOI GRGCYC 1 cut(s) 2072
FspBI CTAG 2 cut(s) 1275, 2136
GlaI GCGC 6 cut(s) 6, 270, 346, 684, 1109, 1591
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 143, 2064
HaeII RGCGCY 4 cut(s) 272, 348, 1111, 1593
HaeIII GGCC 6 cut(s) 206, 218, 260, 444, 616, 1273
HgaI GACGC 3 cut(s) 28, 70, 88
HhaI GCGC 6 cut(s) 7, 271, 347, 685, 1110, 1592
Hin1I GRCGYC 3 cut(s) 39, 62, 345
Hin1II CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
Hin6I GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
HinP1I GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
HincII GTYRAC 2 cut(s) 542, 833
HindII GTYRAC 2 cut(s) 542, 833
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 7 cut(s) 140, 1149, 1185, 1424, 1745, 1784, 1909
HphI GGTGA 7 cut(s) 1380, 1624, 1729, 1938, 1949, 2001, 2064
Hpy166II GTNNAC 7 cut(s) 364, 407, 542, 628, 833, 845, 893
Hpy188I TCNGA 5 cut(s) 641, 1416, 1696, 1822, 2209
Hpy188III TCNNGA 8 cut(s) 137, 469, 776, 912, 1446, 1531, 1706, 1901
Hpy8I GTNNAC 7 cut(s) 364, 407, 542, 628, 833, 845, 893
Hpy99I CGWCG 4 cut(s) 32, 64, 70, 371
HpyCH4III ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
HpyCH4IV ACGT 3 cut(s) 9, 114, 577
HpyF3I CTNAG 8 cut(s) 585, 609, 621, 777, 1125, 1296, 1713, 1875
HpySE526I ACGT 3 cut(s) 9, 114, 577
Hsp92I GRCGYC 3 cut(s) 39, 62, 345
Hsp92II CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
HspAI GCGC 6 cut(s) 5, 269, 345, 683, 1108, 1590
KasI GGCGCC 1 cut(s) 344
KpnI GGTACC 1 cut(s) 922
KroI GCCGGC 2 cut(s) 202, 347
KroNI GCCGGC 2 cut(s) 204, 349
Ksp22I TGATCA 1 cut(s) 742
LmnI GCTCC 4 cut(s) 277, 304, 1115, 2083
Lsp1109I GCAGC 7 cut(s) 107, 292, 694, 1355, 1496, 1655, 2138
LweI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
MaeI CTAG 2 cut(s) 1275, 2136
MaeII ACGT 3 cut(s) 9, 114, 577
MaeIII GTNAC 8 cut(s) 100, 427, 604, 727, 1226, 1386, 1746, 1755
MbiI CCGCTC 2 cut(s) 315, 679
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 1708
MhlI GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
MluNI TGGCCA 1 cut(s) 444
Mly113I GGCGCC 1 cut(s) 345
MlyI GAGTC 3 cut(s) 1158, 1754, 1918
MmeI TCCRAC 3 cut(s) 460, 1151, 1910
Mox20I TGGCCA 1 cut(s) 444
MroNI GCCGGC 2 cut(s) 202, 347
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 444
MseI TTAA 5 cut(s) 1197, 1496, 1730, 1752, 1863
MslI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 4 cut(s) 120, 740, 1509, 1790
MspCI CTTAAG 2 cut(s) 1495, 1751
MspR9I CCNGG 3 cut(s) 173, 248, 1190
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 2 cut(s) 812, 1977
MvaI CCWGG 1 cut(s) 1190
MvnI CGCG 1 cut(s) 242
NaeI GCCGGC 2 cut(s) 204, 349
NarI GGCGCC 1 cut(s) 345
NciI CCSGG 2 cut(s) 173, 248
NcoI CCATGG 2 cut(s) 886, 1244
NdeI CATATG 2 cut(s) 601, 1117
NgoMIV GCCGGC 2 cut(s) 202, 347
NlaIII CATG 9 cut(s) 49, 441, 518, 860, 890, 941, 1248, 1265, 1534
NlaIV GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2179
NmuCI GTSAC 7 cut(s) 100, 427, 727, 1226, 1386, 1746, 1755
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 1645
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 1 cut(s) 1530
PaqCI CACCTGC 1 cut(s) 1847
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 2 cut(s) 812, 1977
PdiI GCCGGC 2 cut(s) 204, 349
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 4 cut(s) 140, 1185, 1424, 1784
PflFI GACNNNGTC 2 cut(s) 38, 64
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 3 cut(s) 1157, 1753, 1917
PluTI GGCGCC 1 cut(s) 348
PpsI GAGTC 3 cut(s) 1157, 1753, 1917
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 93
PshAI GACNNNNGTC 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 1188
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 1 cut(s) 1188
PspN4I GGNNCC 6 cut(s) 346, 615, 920, 1268, 2079, 2179
PspPI GGNCC 6 cut(s) 76, 93, 614, 752, 1267, 2111
PspPPI RGGWCCY 1 cut(s) 93
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 96, 307
PsuI RGATCY 1 cut(s) 1708
PsyI GACNNNGTC 2 cut(s) 38, 64
PvuI CGATCG 1 cut(s) 255
PvuII CAGCTG 4 cut(s) 120, 740, 1509, 1790
RsaI GTAC 3 cut(s) 496, 920, 1080
RsaNI GTAC 3 cut(s) 495, 919, 1079
RseI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
SalI GTCGAC 1 cut(s) 831
SaqAI TTAA 5 cut(s) 1197, 1496, 1730, 1752, 1863
Sau96I GGNCC 6 cut(s) 76, 93, 614, 752, 1267, 2111
SchI GAGTC 3 cut(s) 1158, 1754, 1918
ScrFI CCNGG 3 cut(s) 173, 248, 1190
SduI GDGCHC 5 cut(s) 173, 847, 895, 1561, 2072
SfaNI GCATC 5 cut(s) 253, 558, 1726, 1921, 2095
SfcI CTRYAG 2 cut(s) 88, 1153
SfoI GGCGCC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 1645
SinI GGWCC 5 cut(s) 76, 93, 752, 1267, 2111
SmiMI CAYNNNNRTG 3 cut(s) 578, 1841, 2058
SmlI CTYRAG 2 cut(s) 1495, 1751
SmoI CTYRAG 2 cut(s) 1495, 1751
SnaBI TACGTA 1 cut(s) 578
SphI GCATGC 1 cut(s) 49
Sse9I AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
SsiI CCGC 9 cut(s) 240, 266, 315, 511, 547, 679, 1138, 1521, 1598
SspDI GGCGCC 1 cut(s) 344
SspI AATATT 1 cut(s) 1474
SspMI CTAG 2 cut(s) 1275, 2136
StyD4I CCNGG 3 cut(s) 171, 246, 1188
StyI CCWWGG 3 cut(s) 787, 886, 1244
TaaI ACNGT 6 cut(s) 179, 837, 879, 897, 1411, 1480
TaiI ACGT 3 cut(s) 12, 117, 580
TaqI TCGA 4 cut(s) 255, 832, 1435, 1645
TasI AATT 9 cut(s) 391, 448, 553, 732, 1158, 1502, 1816, 1830, 1972
TauI GCSGC 3 cut(s) 242, 269, 513
TfiI GAWTC 4 cut(s) 140, 1185, 1424, 1784
Tru1I TTAA 5 cut(s) 1197, 1496, 1730, 1752, 1863
Tru9I TTAA 5 cut(s) 1197, 1496, 1730, 1752, 1863
TscAI CASTG 3 cut(s) 167, 842, 1843
TseFI GTSAC 7 cut(s) 100, 427, 727, 1226, 1386, 1746, 1755
TseI GCWGC 7 cut(s) 120, 280, 707, 1368, 1509, 1668, 2126
Tsp45I GTSAC 7 cut(s) 100, 427, 727, 1226, 1386, 1746, 1755
TspDTI ATGAA 4 cut(s) 1248, 1272, 1779, 2052
TspGWI ACGGA 3 cut(s) 12, 45, 290
TspRI CASTG 3 cut(s) 167, 842, 1843
Tth111I GACNNNGTC 2 cut(s) 38, 64
Vha464I CTTAAG 2 cut(s) 1495, 1751
VneI GTGCAC 2 cut(s) 843, 891
VpaK11BI GGWCC 5 cut(s) 76, 93, 752, 1267, 2111
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 1 cut(s) 1502
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 406, 627, 832
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 2 cut(s) 1275, 2136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.