Rroxscaffold_3G00245840

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000644
Physical Location & Seq
Forward (+)
38944303 .. 38949902
5600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245840.1

Sequence Viewer

Length: 2649 bp
ATGGCGCACGTTAGCCAACTGCCGTGCGACGGCGACGGCGTATGCATGCACTGCAAGGCGAAGCCGTCCGACGAGGAGAAGCTGACCTGCAAGACCTGCGTCACGCCGTGGCACGCAGCCTGCCTCGCTCCCTGCTCGCGCCCCGAAACCCTGGCCTCGACGGCGCAGTGGGAGTGCCCGGACTGTACCTCGATCTCCGGCGGGGCTCCGGCGGTGAAGTCCGGCGAGTCCGGGGACTTGATAGCCAAGATCCAGGCGATAATGGCGGACGAGTCGCTGACGGAGGAGGAGAAGGCGAAGAAGCGACAGGAGCTGATGAGCGGAAAGGCGGAGGTGGAGGAGGAGGAGGAGGAAAAGAAAGGAGCGAAGAAGGACGTGTTGGATGTTCTGGATGGGATTTTCAACTGCTCTTTCTGTATGCAGCTGCCGGAGAGACCTGTCACGACACCATGTGGCCACAATTTCTGCCTGAAGTGCTTCCAGAAATGGGTTGGGCAACGAAAGCATACTTGTGCAAAGTGCCGTGCTCCAATTCCTCAAAAGATGGCAAGTCAACCCCGAATCAACTCTTCCCTTGTAATTGCCATACGTATGGCTAGGCTTTCAAAATCACATGTTACTGGGGGGCCTGCCAAGGTATATCATTACATACAAAACCAAGACAGGCCTGACAAAGCATACACTACTGAGCGAGCACAAAAGACTGGAAAAGCTAATGCTGCAAGTGGCAAGATATTTGTTACAATTCCTTCAGATCATTTTGGACCTATACCTGCTGAAAATGATCCCGAGAGGAATCAAGGTGTTATGGTTGGGGAATGTTGGGAGGATAGGCTGGAATGTCGACAATGGGGTGCACACTTTCCCCATGTTTCTGGTATAGCTGGACAGTCTAACCATGGTGCACAGTCTGTTGTGCTTTCGGGAGGGTACCTTGATGATGAGGATCATGGAGAATGGTTCTTATACACAGGAAGCGGCGGAAGAGATTTAAGTGGGAACAAGCGAACTAATAAGGATCAGTCCTTTGACCAGACGTTTGAAAAGTATAACAAGGCGCTCCAAGTTAGTTGCCAAAAAGGCTATCCTGTAAGGGTGGTCAGATCGCACAAGGAGAAGCGCTCCTCATATGCTCCTGAGAAAGGGTTGCGGTATGATGGAGTTTATAGAATTGAGAAGTGTTGGAGAAAGGTGGGAGTCCAGGGGTTTAAGGTCTGCCGGTATCTTTTTGTCAGATGTGACAATGAACCTGCACCATGGACAAGTGATGAACATGGAGATCGGCCTAGACCACTACCTGCAATACCTGAGCTGGCAAAAGCCACAAACATAACAGAGAGGAATGCAACTCCATCATGGGACTTTGAGGAGAAAGATAGCTGCTGGAAATGGATAAAGGCACCACCTATCAGCAAAAAACCTGTTCAAACATTAGATCCTGCTGATCGAAAGAGGTCAAGGAAAGCAATGAGGAAAGCACAGAACATGACTGTACGAGAGAGGCTCTTGAAAGAATTTAGCTGCCTTATTTGTCGCCAGGTGATGACATCACCCGTTACCACATCATGTGGTCATAACTTCTGCAAGTCATGTTTTCAAGGTGCTTTTGTGGGTAAGAGTGTGGTGACAGAAAGAAACAGAGGTGGAAGATCCCTTCGAACTCAAAAGAATGTACTGACCTGCCCTTTCTGTCCCACTGATGTCACTGATTTTGTTCAAAATCCCCAGGTGAACACTGAGATAATGAAAGCAATCGAGTCACTGAAAGAAGAGGAAGCGAATGTGGATTCAGCTGATGAATCAAGTGAAGAAATGGACATTCCTGACAATGAAAATAACACAGAAATGCTTGAGGAAGCAGCTGTGATAGATGGTGAGCAGACTTCTCCAACGAAATGCAACCCCAAAGGCAGTCCCAAACAACTGAAAGTTGATACTGGTAATGACTCGCCAAAGAGTGATATTGTGGAGGTAAATGAAATATCTGCTGTTCAGACTAATGGTGTCAATGGAGATACTGAGATGGAAATTACCAAGGAAAGCAATGAGGTACAGGCTCCAGTTGCGAAACAAGGGAAAAAAACTGCTGGTCGAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAACAAAGAAGACTAAGGAAGCTGTGAGTGAAGAGAACGGCTCACCATCAAGTAATGAAGTTTCTGCTGTACACACTAATGATGTCAATGGAGATTCTGATTTAGATATAGCTAAGGAGACCAATTCTGAGATGGAAATTGCCAAGGAAAGTAATGAGGTACAGGTTCCAGTTGCGAAACGTGGGAAGAAAATTGCTGGGCAAGCTGGCCAAGGTGTTGGAGCAAAGACCAGGGGAAGGAAGAAGGTTAAGGAAGCTGTGAGTGAAGATAACGGCTCTCCATCAAGTAATGAAGTTTCTGCTATCCAGACTAATGGTGTTAATGGAGATTCTGATTTAGATATAGCCAAGGAGAGCAATGAGATACAGGCTCCAGTGGCAAAACGTGGGAAGAAAGCTGCTGCTGTTCTAGCTGTTGAAGGTGTTGGATCAAGGACCAGAGGAAGGAAGAAGGCTAAGGTAGCAGCGAGTGAAGAGAATGGTTCACCTTCTAGTCCTCTCCATGTGGATGTGGATTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

882

Amino Acids

96.38

Weight (kDa)

7.8

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 135 - 179 4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 135 - 175 5.3e-06 Ring finger domain
zf-C3HC4 PF00097 136 - 174 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 136 - 174 3.4e-06 zinc finger of C3HC4-type, RING
SAD_SRA PF02182 266 - 415 5.3e-50 SAD/SRA domain
zf-RING_UBOX PF13445 508 - 532 2.3e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 508 - 533 6.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 6 cut(s) 95, 104, 781, 1258, 1306, 1688
Acc65I GGTACC 1 cut(s) 930
AccB1I GGYRCC 2 cut(s) 930, 1399
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 844
AccII CGCG 1 cut(s) 139
AciI CCGC 8 cut(s) 201, 212, 266, 321, 329, 978, 981, 1150
AclWI GGATC 7 cut(s) 244, 779, 954, 1026, 1430, 1644, 2560
AcoI YGGCCR 3 cut(s) 454, 2098, 2332
AcsI RAATTY 1 cut(s) 1514
AcuI CTGAAG 2 cut(s) 491, 735
AdeI CACNNNGTG 2 cut(s) 108, 452
AfaI GTAC 7 cut(s) 187, 932, 1494, 1674, 2052, 2196, 2286
AfeI AGCGCT 1 cut(s) 1121
AfiI CCNNNNNNNGG 5 cut(s) 29, 487, 1142, 2361, 2568
AflIII ACRYGT 2 cut(s) 375, 613
AgsI TTSAA 8 cut(s) 403, 606, 1043, 1427, 1510, 1598, 1718, 2543
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 7 cut(s) 150, 252, 1200, 1536, 1725, 2120, 2354
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
Alw21I GWGCWC 4 cut(s) 529, 697, 859, 907
Alw26I GTCTC 2 cut(s) 427, 2237
Alw44I GTGCAC 2 cut(s) 855, 903
AlwI GGATC 7 cut(s) 244, 779, 954, 1026, 1430, 1644, 2560
AlwNI CAGNNNCTG 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 788
Aor51HI AGCGCT 1 cut(s) 1121
AoxI GGCC 7 cut(s) 153, 454, 626, 665, 1283, 2098, 2332
ApaLI GTGCAC 2 cut(s) 855, 903
ApoI RAATTY 1 cut(s) 1514
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
Asp700I GAANNNNTTC 1 cut(s) 476
Asp718I GGTACC 1 cut(s) 930
AspLEI GCGC 5 cut(s) 7, 141, 166, 1060, 1122
AspS9I GGNCC 3 cut(s) 626, 764, 2559
AsuC2I CCSGG 2 cut(s) 179, 232
AsuHPI GGTGA 8 cut(s) 226, 1542, 1552, 1636, 1741, 1886, 2160, 2601
AsuII TTCGAA 1 cut(s) 1657
AvaI CYCGRG 1 cut(s) 788
AvaII GGWCC 2 cut(s) 764, 2559
BaeGI GKGCMC 3 cut(s) 179, 859, 907
BalI TGGCCA 3 cut(s) 456, 2100, 2334
BanI GGYRCC 2 cut(s) 930, 1399
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 2141
Bbv12I GWGCWC 4 cut(s) 529, 697, 859, 907
BccI CCATC 9 cut(s) 386, 538, 1151, 1360, 1865, 2017, 2179, 2251, 2413
BceAI ACGGC 9 cut(s) 7, 46, 49, 52, 91, 177, 507, 2179, 2413
BciT130I CCWGG 7 cut(s) 152, 254, 1202, 1538, 1727, 2122, 2356
BcnI CCSGG 2 cut(s) 179, 232
BcoDI GTCTC 2 cut(s) 427, 2237
BfaI CTAG 4 cut(s) 597, 1287, 2534, 2616
BfoI RGCGCY 2 cut(s) 1061, 1123
BfuAI ACCTGC 6 cut(s) 95, 104, 781, 1258, 1306, 1688
BglI GCCNNNNNGGC 2 cut(s) 161, 1080
Bme1390I CCNGG 9 cut(s) 152, 179, 232, 254, 1202, 1538, 1727, 2122, 2356
Bme18I GGWCC 2 cut(s) 764, 2559
BmeT110I CYCGRG 1 cut(s) 788
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 3 cut(s) 626, 764, 2559
BmiI GGNNCC 7 cut(s) 207, 627, 932, 1401, 2058, 2292, 2496
BmrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1202, 1538, 1727, 2122, 2356
BmrI ACTGGG 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 630
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 2141
BplI GAGNNNNNCTC 6 cut(s) 1106, 1138, 1488, 1520, 2150, 2182
BpmI CTGGAG 2 cut(s) 2043, 2481
Bpu10I CCTNAGC 3 cut(s) 1308, 2238, 2580
Bpu14I TTCGAA 1 cut(s) 1657
BpuEI CTTGAG 1 cut(s) 1871
BpuMI CCSGG 2 cut(s) 179, 232
BsaAI YACGTR 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 945
BsaI GGTCTC 2 cut(s) 427, 2237
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 487, 1142, 2361, 2568
Bse118I RCCGGY 1 cut(s) 1218
Bse1I ACTGG 6 cut(s) 625, 709, 1942, 2060, 2294, 2498
Bse3DI GCAATG 3 cut(s) 1473, 2050, 2488
Bse8I GATNNNNATC 1 cut(s) 945
BseBI CCWGG 7 cut(s) 152, 254, 1202, 1538, 1727, 2122, 2356
BseGI GGATG 3 cut(s) 388, 397, 2638
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 5 cut(s) 29, 487, 1142, 2361, 2568
BseMI GCAATG 3 cut(s) 1473, 2050, 2488
BseMII CTCAG 6 cut(s) 678, 1128, 1299, 1728, 2010, 2244
BseNI ACTGG 6 cut(s) 625, 709, 1942, 2060, 2294, 2498
BseRI GAGGAG 9 cut(s) 89, 299, 302, 353, 356, 359, 362, 1114, 1382
BseSI GKGCMC 3 cut(s) 179, 859, 907
BseYI CCCAGC 1 cut(s) 2321
BsgI GTGCAG 1 cut(s) 1236
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 155, 456, 628, 667, 1285, 2100, 2334
BshNI GGYRCC 2 cut(s) 930, 1399
BsiHKAI GWGCWC 4 cut(s) 529, 697, 859, 907
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 7 cut(s) 179, 198, 209, 222, 231, 428, 1219
BslFI GGGAC 4 cut(s) 248, 1373, 1677, 1899
BslI CCNNNNNNNGG 5 cut(s) 29, 487, 1142, 2361, 2568
BsmAI GTCTC 2 cut(s) 427, 2237
BsmFI GGGAC 4 cut(s) 248, 1373, 1677, 1899
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 7 cut(s) 155, 456, 628, 667, 1285, 2100, 2334
Bso31I GGTCTC 2 cut(s) 427, 2237
BsoBI CYCGRG 1 cut(s) 788
Bsp119I TTCGAA 1 cut(s) 1657
Bsp1286I GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 907
Bsp1407I TGTACA 1 cut(s) 2194
Bsp19I CCATGG 2 cut(s) 898, 1256
BspACI CCGC 8 cut(s) 201, 212, 266, 321, 329, 978, 981, 1150
BspANI GGCC 7 cut(s) 155, 456, 628, 667, 1285, 2100, 2334
BspCNI CTCAG 6 cut(s) 679, 1129, 1300, 1729, 2011, 2245
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 7 cut(s) 207, 627, 932, 1401, 2058, 2292, 2496
BspMI ACCTGC 6 cut(s) 95, 104, 781, 1258, 1306, 1688
BspPI GGATC 7 cut(s) 244, 779, 954, 1026, 1430, 1644, 2560
BspT104I TTCGAA 1 cut(s) 1657
BspT107I GGYRCC 2 cut(s) 930, 1399
BspTNI GGTCTC 2 cut(s) 427, 2237
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 3 cut(s) 1473, 2050, 2488
BsrFI RCCGGY 1 cut(s) 1218
BsrGI TGTACA 1 cut(s) 2194
BsrI ACTGG 6 cut(s) 625, 709, 1942, 2060, 2294, 2498
BssAI RCCGGY 1 cut(s) 1218
BssT1I CCWWGG 8 cut(s) 633, 898, 1256, 2034, 2101, 2268, 2335, 2472
Bst2UI CCWGG 7 cut(s) 152, 254, 1202, 1538, 1727, 2122, 2356
Bst4CI ACNGT 4 cut(s) 185, 891, 909, 1492
Bst6I CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
BstAPI GCANNNNNTGC 2 cut(s) 51, 96
BstAUI TGTACA 1 cut(s) 2194
BstBAI YACGTR 1 cut(s) 590
BstBI TTCGAA 1 cut(s) 1657
BstDEI CTNAG 9 cut(s) 687, 1137, 1308, 1737, 2019, 2139, 2238, 2253, 2580
BstDSI CCRYGG 3 cut(s) 107, 898, 1256
BstENI CCTNNNNNAGG 1 cut(s) 1140
BstF5I GGATG 3 cut(s) 388, 397, 2638
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 2 cut(s) 1061, 1123
BstHHI GCGC 5 cut(s) 7, 141, 166, 1060, 1122
BstMAI GTCTC 2 cut(s) 427, 2237
BstNI CCWGG 7 cut(s) 152, 254, 1202, 1538, 1727, 2122, 2356
BstNSI RCATGY 2 cut(s) 49, 617
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 9 cut(s) 150, 177, 230, 252, 1200, 1536, 1725, 2120, 2354
BstSLI GKGCMC 3 cut(s) 179, 859, 907
BstSNI TACGTA 1 cut(s) 590
BstUI CGCG 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 2141
BstX2I RGATCY 3 cut(s) 249, 1435, 1649
BstXI CCANNNNNNTGG 5 cut(s) 592, 875, 2108, 2342, 2438
BstYI RGATCY 3 cut(s) 249, 1435, 1649
BsuRI GGCC 7 cut(s) 155, 456, 628, 667, 1285, 2100, 2334
BtgI CCRYGG 3 cut(s) 107, 898, 1256
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 3 cut(s) 388, 397, 2638
BtsI GCAGTG 2 cut(s) 49, 173
BtsIMutI CAGTG 7 cut(s) 49, 173, 1695, 1704, 1734, 1760, 2505
BveI ACCTGC 6 cut(s) 95, 104, 781, 1258, 1306, 1688
CaiI CAGNNNCTG 1 cut(s) 313
CfoI GCGC 5 cut(s) 7, 141, 166, 1060, 1122
Cfr10I RCCGGY 1 cut(s) 1218
Cfr13I GGNCC 3 cut(s) 626, 764, 2559
CseI GACGC 1 cut(s) 88
Csp6I GTAC 7 cut(s) 186, 931, 1493, 1673, 2051, 2195, 2285
CviQI GTAC 7 cut(s) 186, 931, 1493, 1673, 2051, 2195, 2285
DdeI CTNAG 9 cut(s) 687, 1137, 1308, 1737, 2019, 2139, 2238, 2253, 2580
DraI TTTAAA 1 cut(s) 2644
DraIII CACNNNGTG 2 cut(s) 108, 452
EaeI YGGCCR 3 cut(s) 454, 2098, 2332
Eam1104I CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
EarI CTCTTC 5 cut(s) 574, 979, 1764, 2151, 2592
EciI GGCGGA 3 cut(s) 281, 344, 996
Eco105I TACGTA 1 cut(s) 590
Eco130I CCWWGG 8 cut(s) 633, 898, 1256, 2034, 2101, 2268, 2335, 2472
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 427, 2237
Eco47I GGWCC 2 cut(s) 764, 2559
Eco47III AGCGCT 1 cut(s) 1121
Eco57I CTGAAG 2 cut(s) 491, 735
Eco88I CYCGRG 1 cut(s) 788
EcoNI CCTNNNNNAGG 1 cut(s) 1140
EcoO109I RGGNCCY 1 cut(s) 626
EcoRII CCWGG 7 cut(s) 150, 252, 1200, 1536, 1725, 2120, 2354
EcoT14I CCWWGG 8 cut(s) 633, 898, 1256, 2034, 2101, 2268, 2335, 2472
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 8 cut(s) 633, 898, 1256, 2034, 2101, 2268, 2335, 2472
FaqI GGGAC 4 cut(s) 248, 1373, 1677, 1899
FauI CCCGC 1 cut(s) 194
FauNDI CATATG 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 844
FokI GGATG 3 cut(s) 395, 404, 2645
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 4 cut(s) 597, 1287, 2534, 2616
GlaI GCGC 5 cut(s) 6, 140, 165, 1059, 1121
GsaI CCCAGC 1 cut(s) 2325
GsuI CTGGAG 2 cut(s) 2043, 2481
HaeII RGCGCY 2 cut(s) 1061, 1123
HaeIII GGCC 7 cut(s) 155, 456, 628, 667, 1285, 2100, 2334
HapII CCGG 7 cut(s) 179, 198, 209, 222, 231, 428, 1219
HgaI GACGC 1 cut(s) 88
HhaI GCGC 5 cut(s) 7, 141, 166, 1060, 1122
Hin6I GCGC 5 cut(s) 5, 139, 164, 1058, 1120
HinP1I GCGC 5 cut(s) 5, 139, 164, 1058, 1120
HincII GTYRAC 2 cut(s) 554, 845
HindII GTYRAC 2 cut(s) 554, 845
HpaII CCGG 7 cut(s) 179, 198, 209, 222, 231, 428, 1219
HphI GGTGA 8 cut(s) 226, 1542, 1552, 1636, 1741, 1886, 2160, 2601
Hpy166II GTNNAC 7 cut(s) 554, 845, 857, 905, 1732, 2197, 2609
Hpy188I TCNGA 8 cut(s) 70, 754, 1103, 1235, 1995, 2224, 2254, 2458
Hpy188III TCNNGA 9 cut(s) 389, 442, 481, 788, 924, 1136, 1507, 1823, 2431
Hpy8I GTNNAC 7 cut(s) 554, 845, 857, 905, 1732, 2197, 2609
Hpy99I CGWCG 4 cut(s) 32, 38, 74, 163
HpyCH4III ACNGT 4 cut(s) 185, 891, 909, 1492
HpyCH4IV ACGT 6 cut(s) 9, 375, 589, 1037, 2305, 2509
HpyF3I CTNAG 9 cut(s) 687, 1137, 1308, 1737, 2019, 2139, 2238, 2253, 2580
HpySE526I ACGT 6 cut(s) 9, 375, 589, 1037, 2305, 2509
HspAI GCGC 5 cut(s) 5, 139, 164, 1058, 1120
KpnI GGTACC 1 cut(s) 934
MaeI CTAG 4 cut(s) 597, 1287, 2534, 2616
MaeII ACGT 6 cut(s) 9, 375, 589, 1037, 2305, 2509
MaeIII GTNAC 9 cut(s) 100, 439, 616, 739, 1238, 1555, 1624, 1702, 1758
MbiI CCGCTC 1 cut(s) 321
MflI RGATCY 3 cut(s) 249, 1435, 1649
MhlI GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 907
MlsI TGGCCA 3 cut(s) 456, 2100, 2334
MluNI TGGCCA 3 cut(s) 456, 2100, 2334
MlyI GAGTC 5 cut(s) 236, 281, 1206, 1766, 1940
MmeI TCCRAC 7 cut(s) 93, 360, 1163, 1913, 2089, 2323, 2530
Mox20I TGGCCA 3 cut(s) 456, 2100, 2334
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 3 cut(s) 456, 2100, 2334
MseI TTAA 5 cut(s) 992, 1209, 2373, 2445, 2643
MslI CAYNNNNRTG 5 cut(s) 510, 590, 1844, 2202, 2631
Msp20I TGGCCA 3 cut(s) 456, 2100, 2334
MspA1I CMGCKG 3 cut(s) 424, 1793, 1862
MspI CCGG 7 cut(s) 179, 198, 209, 222, 231, 428, 1219
MspR9I CCNGG 9 cut(s) 152, 179, 232, 254, 1202, 1538, 1727, 2122, 2356
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 7 cut(s) 152, 254, 1202, 1538, 1727, 2122, 2356
MvnI CGCG 1 cut(s) 139
NciI CCSGG 2 cut(s) 179, 232
NcoI CCATGG 2 cut(s) 898, 1256
NdeI CATATG 1 cut(s) 1129
NlaIV GGNNCC 7 cut(s) 207, 627, 932, 1401, 2058, 2292, 2496
NmuCI GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 2 cut(s) 49, 617
NspV TTCGAA 1 cut(s) 1657
PaeI GCATGC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 6 cut(s) 561, 796, 1787, 1799, 2219, 2453
PleI GAGTC 5 cut(s) 235, 280, 1205, 1765, 1940
PpsI GAGTC 5 cut(s) 235, 280, 1205, 1765, 1940
Ppu21I YACGTR 1 cut(s) 590
PscI ACATGT 1 cut(s) 613
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 7 cut(s) 150, 252, 1200, 1536, 1725, 2120, 2354
PspFI CCCAGC 1 cut(s) 2321
PspGI CCWGG 7 cut(s) 150, 252, 1200, 1536, 1725, 2120, 2354
PspN4I GGNNCC 7 cut(s) 207, 627, 932, 1401, 2058, 2292, 2496
PspPI GGNCC 3 cut(s) 626, 764, 2559
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 3 cut(s) 249, 1435, 1649
PvuII CAGCTG 3 cut(s) 424, 1793, 1862
RsaI GTAC 7 cut(s) 187, 932, 1494, 1674, 2052, 2196, 2286
RsaNI GTAC 7 cut(s) 186, 931, 1493, 1673, 2051, 2195, 2285
RseI CAYNNNNRTG 5 cut(s) 510, 590, 1844, 2202, 2631
SalI GTCGAC 1 cut(s) 843
SaqAI TTAA 5 cut(s) 992, 1209, 2373, 2445, 2643
Sau96I GGNCC 3 cut(s) 626, 764, 2559
SchI GAGTC 5 cut(s) 236, 281, 1206, 1766, 1940
ScrFI CCNGG 9 cut(s) 152, 179, 232, 254, 1202, 1538, 1727, 2122, 2356
SduI GDGCHC 6 cut(s) 179, 208, 529, 697, 859, 907
SfuI TTCGAA 1 cut(s) 1657
SinI GGWCC 2 cut(s) 764, 2559
SmiMI CAYNNNNRTG 5 cut(s) 510, 590, 1844, 2202, 2631
SmlI CTYRAG 1 cut(s) 1850
SmoI CTYRAG 1 cut(s) 1850
SnaBI TACGTA 1 cut(s) 590
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 8 cut(s) 201, 212, 266, 321, 329, 978, 981, 1150
SspMI CTAG 4 cut(s) 597, 1287, 2534, 2616
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 9 cut(s) 150, 177, 230, 252, 1200, 1536, 1725, 2120, 2354
StyI CCWWGG 8 cut(s) 633, 898, 1256, 2034, 2101, 2268, 2335, 2472
TaaI ACNGT 4 cut(s) 185, 891, 909, 1492
TaiI ACGT 6 cut(s) 12, 378, 592, 1040, 2308, 2512
TaqI TCGA 7 cut(s) 158, 191, 844, 1447, 1657, 1755, 2092
TatI WGTACW 2 cut(s) 1672, 2194
TauI GCSGC 1 cut(s) 981
TfiI GAWTC 6 cut(s) 561, 796, 1787, 1799, 2219, 2453
Tru1I TTAA 5 cut(s) 992, 1209, 2373, 2445, 2643
Tru9I TTAA 5 cut(s) 992, 1209, 2373, 2445, 2643
TscAI CASTG 7 cut(s) 56, 173, 1702, 1711, 1741, 1767, 2505
TseFI GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
Tsp45I GTSAC 6 cut(s) 100, 439, 1238, 1624, 1702, 1758
TspDTI ATGAA 8 cut(s) 1260, 1284, 1760, 1812, 1845, 1992, 2196, 2430
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 7 cut(s) 56, 173, 1702, 1711, 1741, 1767, 2505
VneI GTGCAC 2 cut(s) 855, 903
VpaK11BI GGWCC 2 cut(s) 764, 2559
XagI CCTNNNNNAGG 1 cut(s) 1140
XapI RAATTY 1 cut(s) 1514
XceI RCATGY 2 cut(s) 49, 617
XcmI CCANNNNNNNNNTGG 2 cut(s) 488, 2254
XmiI GTMKAC 1 cut(s) 844
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 4 cut(s) 597, 1287, 2534, 2616
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.