Rmu_co8479283.1_g000001

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8479283.1
OG0000644
Physical Location & Seq
Forward (+)
1 .. 1976
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8479283.1_g000001.1.cds

Sequence Viewer

Length: 987 bp
acaccatgtggccacaatttctgcttgaagtgcttccagaaatgggttggacaagggaagcgtacttgtgcaaagtgccgccacgccattccttccaaaatggcaagtcaaccccgcatcaattcttcacttgtagttgccatacgtatggctaagatgtcaaaatcatatgttactgaggggcctgctaaggtataccactttgtccgaaaccaagacagacctgacaaagctttcactaccgagcgggcgcagaaaactgggaaatctaatgctgcaagtggcaagatatttgtcacaattccagctgatcattttggacctatacctgctgaatatgatcctgagagaaaccaaggtgtgctggtaggggaatgctgggaggataggcttgaatgtcgacagtggggtgcacactttccccatgttgctggtatagctggacagtctaaccatggtgcacagtcagttgtgctttcaggagggtaccttgatgatgaggatcatggagagtggttcttatacacaggaagtggtggaagggatttgagtgggaataagcgaacaaacaaggatcagtcatttgaccagacatttgagaaatataacaaggctctccaagttagttgcctgaaagggtatcctgtacgggtggtaagatcgcacaaggagaagcgctcttcatatgcccctgagaaagggttgcggtatgatggagtctatagaattgaaaaatgttggacaaaggtgggaatccaggggtttaaggtctgccggtatctttttgttagatgtgacaatgaacctgcaccatggacgagtgatgaacatggggaccggcctagacctttacctccaatacctgagctgaagaaagctacaagtataatagagaggaagtcaagtccatcgtgggactttgaggtaagctattcctttattttactcattggggttcgctgcctagacctaacaaacagatgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

328

Amino Acids

37.03

Weight (kDa)

9.24

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 337, 814
Acc65I GGTACC 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 486
AccBSI CCGCTC 1 cut(s) 247
AccI GTMKAC 2 cut(s) 195, 400
AciI CCGC 4 cut(s) 79, 115, 247, 706
AclWI GGATC 3 cut(s) 335, 510, 582
AcoI YGGCCR 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 890
AdeI CACNNNGTG 1 cut(s) 8
AfaI GTAC 3 cut(s) 64, 488, 648
AfeI AGCGCT 1 cut(s) 677
AfiI CCNNNNNNNGG 2 cut(s) 43, 698
AgsI TTSAA 3 cut(s) 28, 395, 731
AjnI CCWGG 1 cut(s) 756
AluBI AGCT 6 cut(s) 233, 308, 440, 868, 878, 930
AluI AGCT 6 cut(s) 233, 308, 440, 868, 878, 930
Alw21I GWGCWC 2 cut(s) 415, 463
Alw44I GTGCAC 2 cut(s) 411, 459
AlwI GGATC 3 cut(s) 335, 510, 582
Aor51HI AGCGCT 1 cut(s) 677
AoxI GGCC 3 cut(s) 10, 182, 839
ApaLI GTGCAC 2 cut(s) 411, 459
ApeKI GCWGC 2 cut(s) 275, 960
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp700I GAANNNNTTC 1 cut(s) 32
Asp718I GGTACC 1 cut(s) 486
AspLEI GCGC 2 cut(s) 253, 678
AspS9I GGNCC 3 cut(s) 182, 320, 835
AvaII GGWCC 2 cut(s) 320, 835
BaeGI GKGCMC 2 cut(s) 415, 463
BalI TGGCCA 1 cut(s) 12
BanI GGYRCC 1 cut(s) 486
Bbv12I GWGCWC 2 cut(s) 415, 463
BbvI GCAGC 2 cut(s) 262, 947
BccI CCATC 2 cut(s) 707, 916
BciT130I CCWGG 1 cut(s) 758
BciVI GTATCC 1 cut(s) 651
BclI TGATCA 1 cut(s) 310
BfaI CTAG 2 cut(s) 843, 965
BfmI CTRYAG 1 cut(s) 721
BfoI RGCGCY 1 cut(s) 679
BfuAI ACCTGC 2 cut(s) 337, 814
BfuI GTATCC 1 cut(s) 651
BisI GCNGC 3 cut(s) 79, 276, 961
BlsI GCNGC 3 cut(s) 80, 277, 962
Bme1390I CCNGG 1 cut(s) 758
Bme18I GGWCC 2 cut(s) 320, 835
BmgT120I GGNCC 3 cut(s) 182, 320, 835
BmiI GGNNCC 3 cut(s) 183, 488, 836
BmrFI CCNGG 1 cut(s) 758
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 662, 694
Bpu10I CCTNAGC 2 cut(s) 189, 864
BsaAI YACGTR 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 501
BsaJI CCNNGG 4 cut(s) 355, 454, 757, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 698
Bse118I RCCGGY 2 cut(s) 774, 837
Bse1I ACTGG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 1 cut(s) 758
BseDI CCNNGG 4 cut(s) 355, 454, 757, 812
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 43, 698
BseMII CTCAG 4 cut(s) 168, 336, 684, 855
BseNI ACTGG 1 cut(s) 265
BseSI GKGCMC 2 cut(s) 415, 463
BseXI GCAGC 2 cut(s) 262, 947
BseYI CCCAGC 1 cut(s) 378
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 3 cut(s) 12, 184, 841
BshNI GGYRCC 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 415, 463
BsiSI CCGG 2 cut(s) 775, 838
BslFI GGGAC 2 cut(s) 848, 929
BslI CCNNNNNNNGG 2 cut(s) 43, 698
BsmFI GGGAC 2 cut(s) 848, 929
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 3 cut(s) 12, 184, 841
Bsp1286I GDGCHC 2 cut(s) 415, 463
Bsp143I GATC 5 cut(s) 310, 340, 502, 574, 659
Bsp19I CCATGG 2 cut(s) 454, 812
BspACI CCGC 4 cut(s) 79, 115, 247, 706
BspANI GGCC 3 cut(s) 12, 184, 841
BspCNI CTCAG 4 cut(s) 169, 337, 685, 856
BspLI GGNNCC 3 cut(s) 183, 488, 836
BspMI ACCTGC 2 cut(s) 337, 814
BspPI GGATC 3 cut(s) 335, 510, 582
BspQI GCTCTTC 1 cut(s) 685
BspT107I GGYRCC 1 cut(s) 486
BsrBI CCGCTC 1 cut(s) 247
BsrFI RCCGGY 2 cut(s) 774, 837
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 2 cut(s) 774, 837
BssECI CCNNGG 4 cut(s) 355, 454, 757, 812
BssMI GATC 5 cut(s) 310, 340, 502, 574, 659
BssNAI GTATAC 1 cut(s) 196
BssT1I CCWWGG 3 cut(s) 355, 454, 812
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 758
Bst4CI ACNGT 3 cut(s) 405, 447, 465
Bst6I CTCTTC 1 cut(s) 685
BstBAI YACGTR 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 186, 249
BstDEI CTNAG 6 cut(s) 153, 177, 189, 345, 693, 864
BstDSI CCRYGG 2 cut(s) 454, 812
BstENI CCTNNNNNAGG 1 cut(s) 696
BstH2I RGCGCY 1 cut(s) 679
BstHHI GCGC 2 cut(s) 253, 678
BstKTI GATC 5 cut(s) 313, 343, 505, 577, 662
BstMBI GATC 5 cut(s) 310, 340, 502, 574, 659
BstMWI GCNNNNNNNGC 2 cut(s) 30, 437
BstNI CCWGG 1 cut(s) 758
BstSCI CCNGG 1 cut(s) 756
BstSFI CTRYAG 1 cut(s) 721
BstSLI GKGCMC 2 cut(s) 415, 463
BstSNI TACGTA 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 262, 947
BstXI CCANNNNNNTGG 2 cut(s) 148, 431
BstZ17I GTATAC 1 cut(s) 196
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 3 cut(s) 12, 184, 841
BtgI CCRYGG 2 cut(s) 454, 812
BtsIMutI CAGTG 1 cut(s) 410
BveI ACCTGC 2 cut(s) 337, 814
Cac8I GCNNGC 2 cut(s) 186, 249
CfoI GCGC 2 cut(s) 253, 678
Cfr10I RCCGGY 2 cut(s) 774, 837
Cfr13I GGNCC 3 cut(s) 182, 320, 835
Csp6I GTAC 3 cut(s) 63, 487, 647
CviAII CATG 6 cut(s) 6, 425, 455, 506, 813, 830
CviQI GTAC 3 cut(s) 63, 487, 647
DdeI CTNAG 6 cut(s) 153, 177, 189, 345, 693, 864
DpnI GATC 5 cut(s) 312, 342, 504, 576, 661
DpnII GATC 5 cut(s) 310, 340, 502, 574, 659
DraIII CACNNNGTG 1 cut(s) 8
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 685
Eco105I TACGTA 1 cut(s) 146
Eco130I CCWWGG 3 cut(s) 355, 454, 812
Eco47I GGWCC 2 cut(s) 320, 835
Eco47III AGCGCT 1 cut(s) 677
Eco57I CTGAAG 1 cut(s) 890
EcoNI CCTNNNNNAGG 1 cut(s) 696
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 756
EcoT14I CCWWGG 3 cut(s) 355, 454, 812
ErhI CCWWGG 3 cut(s) 355, 454, 812
FaeI CATG 6 cut(s) 9, 428, 458, 509, 816, 833
FaqI GGGAC 2 cut(s) 848, 929
FatI CATG 6 cut(s) 5, 424, 454, 505, 812, 829
FauI CCCGC 2 cut(s) 122, 240
FauNDI CATATG 2 cut(s) 169, 685
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 2 cut(s) 195, 400
Fnu4HI GCNGC 3 cut(s) 79, 276, 961
Fsp4HI GCNGC 3 cut(s) 79, 276, 961
FspBI CTAG 2 cut(s) 843, 965
GlaI GCGC 2 cut(s) 252, 677
GluI GCNGC 3 cut(s) 79, 276, 961
GsaI CCCAGC 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 679
HaeIII GGCC 3 cut(s) 12, 184, 841
HapII CCGG 2 cut(s) 775, 838
HhaI GCGC 2 cut(s) 253, 678
Hin1II CATG 6 cut(s) 9, 428, 458, 509, 816, 833
Hin6I GCGC 2 cut(s) 251, 676
HinP1I GCGC 2 cut(s) 251, 676
HincII GTYRAC 2 cut(s) 110, 401
HindII GTYRAC 2 cut(s) 110, 401
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 2 cut(s) 717, 753
HpaII CCGG 2 cut(s) 775, 838
Hpy166II GTNNAC 5 cut(s) 110, 196, 401, 413, 461
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 3 cut(s) 37, 344, 480
Hpy8I GTNNAC 5 cut(s) 110, 196, 401, 413, 461
HpyAV CCTTC 2 cut(s) 102, 534
HpyCH4III ACNGT 3 cut(s) 405, 447, 465
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 71, 278, 413, 461, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 437
HpyF3I CTNAG 6 cut(s) 153, 177, 189, 345, 693, 864
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 6 cut(s) 9, 428, 458, 509, 816, 833
HspAI GCGC 2 cut(s) 251, 676
KpnI GGTACC 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 5 cut(s) 310, 340, 502, 574, 659
LguI GCTCTTC 1 cut(s) 685
Lsp1109I GCAGC 2 cut(s) 262, 947
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 843, 965
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 3 cut(s) 172, 295, 794
MalI GATC 5 cut(s) 312, 342, 504, 576, 661
MbiI CCGCTC 1 cut(s) 247
MboI GATC 5 cut(s) 310, 340, 502, 574, 659
MboII GAAGA 3 cut(s) 117, 672, 883
MhlI GDGCHC 2 cut(s) 415, 463
MlsI TGGCCA 1 cut(s) 12
MluCI AATT 4 cut(s) 16, 121, 300, 726
MluNI TGGCCA 1 cut(s) 12
MlyI GAGTC 1 cut(s) 726
MmeI TCCRAC 2 cut(s) 28, 719
MnlI CCTC 7 cut(s) 172, 376, 476, 493, 864, 888, 916
Mox20I TGGCCA 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 32
MscI TGGCCA 1 cut(s) 12
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 775, 838
MspR9I CCNGG 1 cut(s) 758
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 1 cut(s) 758
MwoI GCNNNNNNNGC 2 cut(s) 30, 437
NcoI CCATGG 2 cut(s) 454, 812
NdeI CATATG 2 cut(s) 169, 685
NdeII GATC 5 cut(s) 310, 340, 502, 574, 659
NlaIII CATG 6 cut(s) 9, 428, 458, 509, 816, 833
NlaIV GGNNCC 3 cut(s) 183, 488, 836
NmuCI GTSAC 2 cut(s) 295, 794
PciSI GCTCTTC 1 cut(s) 685
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 753
PkrI GCNGC 3 cut(s) 80, 277, 962
PleI GAGTC 1 cut(s) 725
PpsI GAGTC 1 cut(s) 725
Ppu21I YACGTR 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 756
PspFI CCCAGC 1 cut(s) 378
PspGI CCWGG 1 cut(s) 756
PspN4I GGNNCC 3 cut(s) 183, 488, 836
PspPI GGNCC 3 cut(s) 182, 320, 835
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 64, 488, 648
RsaNI GTAC 3 cut(s) 63, 487, 647
RseI CAYNNNNRTG 1 cut(s) 146
SalI GTCGAC 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 685
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 3 cut(s) 79, 276, 961
Sau3AI GATC 5 cut(s) 310, 340, 502, 574, 659
Sau96I GGNCC 3 cut(s) 182, 320, 835
SchI GAGTC 1 cut(s) 726
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 2 cut(s) 415, 463
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 721
SinI GGWCC 2 cut(s) 320, 835
SmiMI CAYNNNNRTG 1 cut(s) 146
SnaBI TACGTA 1 cut(s) 146
Sse9I AATT 4 cut(s) 16, 121, 300, 726
SsiI CCGC 4 cut(s) 79, 115, 247, 706
SspMI CTAG 2 cut(s) 843, 965
StyD4I CCNGG 1 cut(s) 756
StyI CCWWGG 3 cut(s) 355, 454, 812
TaaI ACNGT 3 cut(s) 405, 447, 465
TaiI ACGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 400
TasI AATT 4 cut(s) 16, 121, 300, 726
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 753
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 2 cut(s) 295, 794
TseI GCWGC 2 cut(s) 275, 960
Tsp45I GTSAC 2 cut(s) 295, 794
TspDTI ATGAA 3 cut(s) 672, 816, 840
TspRI CASTG 1 cut(s) 410
VneI GTGCAC 2 cut(s) 411, 459
VpaK11BI GGWCC 2 cut(s) 320, 835
XagI CCTNNNNNAGG 1 cut(s) 696
XcmI CCANNNNNNNNNTGG 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 195, 400
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 843, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.