RchiOBHm_Chr6g0248811

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000644
Physical Location & Seq
Reverse (-)
4716615 .. 4717826
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22303

Sequence Viewer

Length: 708 bp
ATGAATGAGTATCATTTACAGCATGCATTTAATGCACGCCGTAAATTTAGCGTCACACCTTGCGGGCACAACTTCTGCCTCAAATGCTTTGAGAAATGGGTGGCGCAGGGTAAACTTACATGTGCAAATTGCCGCCAATCGATCCCCTCCACTCTGAGAATCAACTCTAATCATGTGGGAAGCATTCGTATTGCCAAGGCATCACAACGCAGCGGCTCAGGGGAGTACCTACCTCAGTTGGCTCATCGCATTCGAAACCAGGACCGTCCAGACAAAGCATTTACAACCGAGCGAGCCAAGAAGCCTGGGAAAGCTAATGCCTCAAGTGGAAGGATCTTTGTGACATCAGCTCCGGATCATTTTGGGCCCATAACAGCCGAATATGATCCTGAGAAGAATAAGGGTGTGGCGGTGGGGGAGACTTGGGGGCATAGGATTGAATGTAGACAATGGGGAATTCATCGCCCTATGGTTTCCGGGATTTCTGGGCAGTGCAAGTATGGCGCCCAATCAGTGGTTCTCTCAGGAGGGTATATAGACGATGAAGATCACGGTGAATGGTTCATTTATACCGGGAGTGGTGGCAAGGATTTGAGTCGAAACAAGCGTACCAACAAGCAACACAGTAAAGATCAGACGTTCACAAAGCTTAATCAGTCACTCAGGCTAAGTTGCCTAAATGGTTATCCTGTTCGAGTAGTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

235

Amino Acids

26.34

Weight (kDa)

9.78

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 10 - 45 2.1e-06 Ring finger domain
zf-C3HC4_3 PF13920 12 - 50 4.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 16 - 44 1.6e-06 Zinc finger, C3HC4 type (RING finger)
SAD_SRA PF02182 133 - 235 7e-33 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 514
AccI GTMKAC 1 cut(s) 445
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 4 cut(s) 63, 133, 213, 410
AclWI GGATC 4 cut(s) 136, 341, 363, 380
AcsI RAATTY 2 cut(s) 44, 456
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 2 cut(s) 227, 610
AfiI CCNNNNNNNGG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 1 cut(s) 440
AjnI CCWGG 2 cut(s) 258, 304
AjuI GAANNNNNNNTTGG 2 cut(s) 501, 533
AluBI AGCT 3 cut(s) 314, 350, 649
AluI AGCT 3 cut(s) 314, 350, 649
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 4 cut(s) 136, 341, 363, 380
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 1 cut(s) 365
ApaI GGGCCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 2 cut(s) 44, 456
AspLEI GCGC 2 cut(s) 106, 506
AspS9I GGNCC 3 cut(s) 262, 365, 366
AsuC2I CCSGG 2 cut(s) 478, 574
AsuHPI GGTGA 1 cut(s) 566
AsuII TTCGAA 1 cut(s) 253
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 2 cut(s) 69, 369
BaeI ACNNNNGTAYC 2 cut(s) 592, 625
BanI GGYRCC 1 cut(s) 503
BanII GRGCYC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 222
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 260, 306
BcnI CCSGG 2 cut(s) 478, 574
BcoDI GTCTC 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 507
BisI GCNGC 3 cut(s) 133, 211, 214
BlsI GCNGC 3 cut(s) 134, 212, 215
Bme1390I CCNGG 4 cut(s) 260, 306, 478, 574
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 3 cut(s) 262, 365, 366
BmiI GGNNCC 2 cut(s) 367, 505
BmrFI CCNGG 4 cut(s) 260, 306, 478, 574
BmsI GCATC 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 307
BpuMI CCSGG 2 cut(s) 478, 574
Bsa29I ATCGAT 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 195, 305
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 546
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 2 cut(s) 260, 306
BseCI ATCGAT 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 195, 305
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMII CTCAG 6 cut(s) 146, 231, 248, 381, 537, 676
BseSI GKGCMC 2 cut(s) 69, 369
BseXI GCAGC 1 cut(s) 222
BshFI GGCC 1 cut(s) 367
BshNI GGYRCC 1 cut(s) 503
BshVI ATCGAT 1 cut(s) 140
BsiSI CCGG 3 cut(s) 353, 477, 573
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 413
BsmI GAATGC 2 cut(s) 183, 249
BsnI GGCC 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 253
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 69, 369
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 6 cut(s) 141, 333, 355, 385, 547, 631
BspACI CCGC 4 cut(s) 63, 133, 213, 410
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 6 cut(s) 147, 230, 247, 382, 536, 675
BspDI ATCGAT 1 cut(s) 140
BspEI TCCGGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 367, 505
BspPI GGATC 4 cut(s) 136, 341, 363, 380
BspT104I TTCGAA 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 503
BssECI CCNNGG 2 cut(s) 195, 305
BssMI GATC 6 cut(s) 141, 333, 355, 385, 547, 631
BssNI GRCGYC 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 260, 306
Bst4CI ACNGT 3 cut(s) 266, 554, 626
BstACI GRCGYC 1 cut(s) 504
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 253
BstC8I GCNNGC 4 cut(s) 24, 37, 65, 294
BstDEI CTNAG 7 cut(s) 155, 217, 234, 390, 523, 662, 668
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 2 cut(s) 106, 506
BstKTI GATC 6 cut(s) 144, 336, 358, 388, 550, 634
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 6 cut(s) 141, 333, 355, 385, 547, 631
BstMWI GCNNNNNNNGC 3 cut(s) 32, 84, 501
BstNI CCWGG 2 cut(s) 260, 306
BstNSI RCATGY 2 cut(s) 26, 123
BstSCI CCNGG 4 cut(s) 258, 304, 476, 572
BstSLI GKGCMC 2 cut(s) 69, 369
BstV1I GCAGC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 140
BsuRI GGCC 1 cut(s) 367
BsuTUI ATCGAT 1 cut(s) 140
BtgZI GCGATG 2 cut(s) 230, 446
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 2 cut(s) 497, 519
Cac8I GCNNGC 4 cut(s) 24, 37, 65, 294
CfoI GCGC 2 cut(s) 106, 506
Cfr13I GGNCC 3 cut(s) 262, 365, 366
ClaI ATCGAT 1 cut(s) 140
CseI GACGC 1 cut(s) 40
Csp6I GTAC 2 cut(s) 226, 609
CviAII CATG 3 cut(s) 23, 120, 173
CviQI GTAC 2 cut(s) 226, 609
DdeI CTNAG 7 cut(s) 155, 217, 234, 390, 523, 662, 668
DinI GGCGCC 1 cut(s) 505
DpnI GATC 6 cut(s) 143, 335, 357, 387, 549, 633
DpnII GATC 6 cut(s) 141, 333, 355, 385, 547, 631
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 2 cut(s) 258, 304
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 369
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 26, 123, 176
FatI CATG 3 cut(s) 22, 119, 172
FauI CCCGC 1 cut(s) 56
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 3 cut(s) 133, 211, 214
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 133, 211, 214
GlaI GCGC 2 cut(s) 105, 505
GluI GCNGC 3 cut(s) 133, 211, 214
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 1 cut(s) 367
HapII CCGG 3 cut(s) 353, 477, 573
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 106, 506
Hin1I GRCGYC 1 cut(s) 504
Hin1II CATG 3 cut(s) 26, 123, 176
Hin6I GCGC 2 cut(s) 104, 504
HinP1I GCGC 2 cut(s) 104, 504
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 2 cut(s) 159, 595
HpaII CCGG 3 cut(s) 353, 477, 573
HphI GGTGA 1 cut(s) 566
Hpy166II GTNNAC 3 cut(s) 113, 446, 642
Hpy188I TCNGA 2 cut(s) 156, 636
Hpy188III TCNNGA 4 cut(s) 269, 353, 389, 525
Hpy8I GTNNAC 3 cut(s) 113, 446, 642
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 3 cut(s) 266, 554, 626
HpyCH4IV ACGT 1 cut(s) 638
HpyCH4V TGCA 4 cut(s) 26, 35, 125, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 84, 501
HpyF3I CTNAG 7 cut(s) 155, 217, 234, 390, 523, 662, 668
HpySE526I ACGT 1 cut(s) 638
Hsp92I GRCGYC 1 cut(s) 504
Hsp92II CATG 3 cut(s) 26, 123, 176
HspAI GCGC 2 cut(s) 104, 504
KasI GGCGCC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 352
Kzo9I GATC 6 cut(s) 141, 333, 355, 385, 547, 631
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 1 cut(s) 209
MaeII ACGT 1 cut(s) 638
MaeIII GTNAC 3 cut(s) 52, 340, 657
MalI GATC 6 cut(s) 143, 335, 357, 387, 549, 633
MboI GATC 6 cut(s) 141, 333, 355, 385, 547, 631
MboII GAAGA 2 cut(s) 406, 557
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 2 cut(s) 69, 369
MluCI AATT 3 cut(s) 44, 127, 456
Mly113I GGCGCC 1 cut(s) 504
MlyI GAGTC 1 cut(s) 604
MnlI CCTC 6 cut(s) 89, 157, 243, 331, 521, 696
Mph1103I ATGCAT 1 cut(s) 28
MroI TCCGGA 1 cut(s) 352
MseI TTAA 2 cut(s) 30, 651
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 3 cut(s) 353, 477, 573
MspR9I CCNGG 4 cut(s) 260, 306, 478, 574
Mva1269I GAATGC 2 cut(s) 183, 249
MvaI CCWGG 2 cut(s) 260, 306
MwoI GCNNNNNNNGC 3 cut(s) 32, 84, 501
NarI GGCGCC 1 cut(s) 504
NciI CCSGG 2 cut(s) 478, 574
NdeII GATC 6 cut(s) 141, 333, 355, 385, 547, 631
NlaIII CATG 3 cut(s) 26, 123, 176
NlaIV GGNNCC 2 cut(s) 367, 505
NmuCI GTSAC 3 cut(s) 52, 340, 657
NsiI ATGCAT 1 cut(s) 28
NspI RCATGY 2 cut(s) 26, 123
NspV TTCGAA 1 cut(s) 253
PaeI GCATGC 1 cut(s) 26
PciI ACATGT 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 604
PctI GAATGC 2 cut(s) 183, 249
PfeI GAWTC 1 cut(s) 159
PflMI CCANNNNNTGG 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 476
PkrI GCNGC 3 cut(s) 134, 212, 215
PleI GAGTC 1 cut(s) 603
PluTI GGCGCC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 603
PscI ACATGT 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 258, 304
PspGI CCWGG 2 cut(s) 258, 304
PspN4I GGNNCC 2 cut(s) 367, 505
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 3 cut(s) 262, 365, 366
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 2 cut(s) 227, 610
RsaNI GTAC 2 cut(s) 226, 609
SaqAI TTAA 2 cut(s) 30, 651
SatI GCNGC 3 cut(s) 133, 211, 214
Sau3AI GATC 6 cut(s) 141, 333, 355, 385, 547, 631
Sau96I GGNCC 3 cut(s) 262, 365, 366
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 4 cut(s) 260, 306, 478, 574
SduI GDGCHC 2 cut(s) 69, 369
SetI ASST 8 cut(s) 61, 231, 235, 316, 352, 641, 651, 707
SfaNI GCATC 1 cut(s) 209
SfoI GGCGCC 1 cut(s) 505
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
SphI GCATGC 1 cut(s) 26
Sse9I AATT 3 cut(s) 44, 127, 456
SsiI CCGC 4 cut(s) 63, 133, 213, 410
SspDI GGCGCC 1 cut(s) 503
StyD4I CCNGG 4 cut(s) 258, 304, 476, 572
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 3 cut(s) 266, 554, 626
TaiI ACGT 1 cut(s) 641
TaqI TCGA 4 cut(s) 140, 253, 598, 694
TasI AATT 3 cut(s) 44, 127, 456
TauI GCSGC 2 cut(s) 135, 216
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 2 cut(s) 30, 651
Tru9I TTAA 2 cut(s) 30, 651
TscAI CASTG 2 cut(s) 497, 519
TseFI GTSAC 3 cut(s) 52, 340, 657
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 3 cut(s) 52, 340, 657
TspDTI ATGAA 4 cut(s) 17, 449, 553, 558
TspRI CASTG 2 cut(s) 497, 519
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 2 cut(s) 44, 456
XceI RCATGY 2 cut(s) 26, 123
XmiI GTMKAC 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.